Biology Meiosis Chapter Quiz
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Questions and Answers

During which stage of Prophase I does homologous recombination, or crossing-over, occur, leading to the exchange of DNA segments between non-sister chromatids?

  • Zygotene
  • Diplotene
  • Pachytene (correct)
  • Leptotene
  • What is the primary role of the synaptonemal complex during Zygotene?

  • To initiate the condensation of chromatin into chromosomes.
  • To connect homologous chromosomes into bivalents, facilitating synapsis. (correct)
  • To promote the physical exchange of alleles via crossing-over.
  • To facilitate the movement of cell organelles to the periphery of the cell.
  • In which stage of Prophase I do chiasmata become visible as homologous chromosomes begin to separate, but chromatid pairs remain connected?

  • Zygotene
  • Diplotene (correct)
  • Leptotene
  • Pachytene
  • If a cell in Pachytene has 3 bivalents, and each bivalent forms an average of 2 chiasmata, how many crossing-over events are likely to occur?

    <p>6 (B)</p> Signup and view all the answers

    What is the fundamental difference between mitosis and meiosis in eukaryotic cells?

    <p>Mitosis results in daughter cells with the same number of chromosomes as the parent cell, while meiosis results in daughter cells with half the number of chromosomes. (C)</p> Signup and view all the answers

    Considering a human cell undergoing meiosis, what would be the outcome if a chromosome segregation error occurred during meiosis I, resulting in one daughter cell receiving both homologous chromosomes for chromosome 21?

    <p>One daughter cell would have an extra copy of chromosome 21 (trisomy 21), and the other would be missing a copy (monosomy 21). (D)</p> Signup and view all the answers

    Which event is characteristic of the Leptotene stage of Prophase I?

    <p>The condensation of chromatin to form visible chromosomes. (D)</p> Signup and view all the answers

    Which is the correct pairing of cell type and number of chromosomes?

    <p>Human somatic cell: 46 chromosomes (2n) (D)</p> Signup and view all the answers

    How does the chromosome number change during the transition from a diploid cell to a haploid cell?

    <p>The chromosome number is halved. (D)</p> Signup and view all the answers

    If a diploid cell from an organism has 24 chromosomes, how many chromosomes would be present in a haploid cell from the same organism?

    <p>12 (D)</p> Signup and view all the answers

    What is the primary role of the Cyclin A-Cdk2 complex during the S phase of the cell cycle?

    <p>Initiating the pre-replication complex to allow one round of DNA replication and preventing further rounds. (D)</p> Signup and view all the answers

    How does the MPF complex contribute to the progression of a cell through the M phase?

    <p>By activating proteins in the nuclear envelope, leading to its disintegration, and promoting chromosome condensation. (D)</p> Signup and view all the answers

    Which event is most critical in the early stages of the M phase and is directly influenced by the MPF complex?

    <p>The breakdown of the nuclear envelope. (D)</p> Signup and view all the answers

    What crucial mechanism ensures the activation of the MPF complex, facilitating a cell's entry into the M phase?

    <p>The removal of phosphate groups by Cdc25 phosphatase. (C)</p> Signup and view all the answers

    How does the MPF complex activation process amplify itself to ensure a robust transition into the M phase?

    <p>By indirectly activating more MPF complexes, creating a positive-feedback loop. (C)</p> Signup and view all the answers

    What is the direct consequence of the APC/C complex ubiquitinating cyclin?

    <p>Cyclin is targeted for degradation by the proteasome. (B)</p> Signup and view all the answers

    How does the activation of p53 protein contribute to cell cycle arrest in response to DNA damage?

    <p>It promotes transcription of the p21 gene, which inhibits G1/S-Cdk and S-Cdk complexes. (C)</p> Signup and view all the answers

    What is the role of separase once it is released from the securin-separase complex?

    <p>It cleaves cohesins, allowing sister chromatids to separate. (B)</p> Signup and view all the answers

    What is the immediate consequence of securin degradation by the APC/C complex?

    <p>Release of active separase. (A)</p> Signup and view all the answers

    Which of the following events marks the end of the M phase and allows new daughter cells to enter the G1 phase?

    <p>The degradation of the MPF complex by ubiquitination via the APC/C. (D)</p> Signup and view all the answers

    How do INK4 proteins inhibit the cell cycle progression?

    <p>By binding to and inhibiting the activity of Cdk4 and Cdk6 complexes. (D)</p> Signup and view all the answers

    What role does the protein p27 play in the cell cycle?

    <p>It determines whether a cell enters a new cycle or goes into a resting phase, by binding to E-Cdk2 and A-Cdk2 complexes. (C)</p> Signup and view all the answers

    Under what circumstances would a cell typically undergo apoptosis?

    <p>When it is damaged, infected, or laden with mutations, to maintain tissue homeostasis. (D)</p> Signup and view all the answers

    How does necrosis differ fundamentally from apoptosis?

    <p>Necrosis is a passive process induced by external factors, often leading to inflammation, while apoptosis is an active, programmed process. (A)</p> Signup and view all the answers

    What is the immediate consequence of a cell undergoing necrosis?

    <p>Swelling of the cell and leakage of its contents into the extracellular space, triggering an inflammatory response. (A)</p> Signup and view all the answers

    During Diakinesis, which event is characteristically observed regarding the chiasmata?

    <p>Chiasmata shift toward the ends of the chromosomes. (D)</p> Signup and view all the answers

    What is the crucial event that defines Anaphase I of meiosis?

    <p>The separation of homologous chromosomes. (B)</p> Signup and view all the answers

    What is the significance of chromosome decondensation during Telophase I?

    <p>It facilitates gene expression and cellular activity. (D)</p> Signup and view all the answers

    How does Meiosis II differ from Mitosis?

    <p>Meiosis II starts with haploid cells, whereas mitosis starts with diploid or haploid cells. (C)</p> Signup and view all the answers

    During Metaphase II, what ensures proper chromosome segregation?

    <p>Each sister chromatid is attached to spindle fibers from opposite poles. (A)</p> Signup and view all the answers

    What is the immediate outcome of Anaphase II?

    <p>Sister chromatids are separated and become daughter chromosomes. (B)</p> Signup and view all the answers

    What is the primary purpose of cell cycle checkpoints?

    <p>To ensure the correct sequence of cell cycle events and prevent errors. (C)</p> Signup and view all the answers

    What cellular process is directly monitored at the S checkpoint?

    <p>Integrity of replicated DNA and presence of replication forks. (D)</p> Signup and view all the answers

    How do cell cycle regulators typically exert their control?

    <p>By cyclically activating and inhibiting proteins and protein complexes. (A)</p> Signup and view all the answers

    Consider a scenario where a cell has passed the G1 checkpoint but then encounters significant DNA damage during the S phase. What is the likely cellular response?

    <p>The S checkpoint will halt the cell cycle to allow for DNA repair. (C)</p> Signup and view all the answers

    Flashcards

    Leptotene

    Stage in Prophase I where chromatin condenses into chromosomes; each has two sister chromatids.

    Zygotene

    Stage in Prophase I where homologous chromosomes pair to form bivalents/tetrads and the synaptonemal complex forms.

    Pachytene

    Stage in Prophase I where chromosomes condense further and crossing-over occurs at chiasmata between non-sister chromatids.

    Crossing-over

    Process where homologous recombination occurs, exchanging DNA segments between non-sister chromatids during Pachytene.

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    Diplotene

    Stage in Prophase I where crossing-over finishes and homologous chromosomes begin to separate, connected by chiasma.

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    Diploid cells

    Cells containing a double set of chromosomes, denoted as 2n.

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    Haploid cells

    Cells containing a single set of chromosomes, denoted as 1n.

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    Mitosis

    A type of cell division where daughter cells receive the same number of chromosomes as the parent.

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    Meiosis

    A type of cell division where daughter cells receive half the number of chromosomes.

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    Cell Cycle

    A series of processes in eukaryotic cells that lead to cell division.

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    Cyclin A and Cdk2

    A complex that activates DNA replication at the end of G1 phase and transitions to S phase.

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    S phase

    The phase where DNA is replicated once, allowing transition to G2 phase.

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    G2 phase

    The phase where cyclin A binds Cdk1 to prepare the cell for M phase, and cyclin B is inactive.

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    M-phase Promoting Factor (MPF)

    The cyclin B-Cdk1 complex that controls mitosis and activates key mitotic processes.

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    Positive-feedback loop in MPF activation

    Activated MPF stimulates more MPF activation, enhancing the process.

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    APC/C

    Anaphase-promoting complex/cyclosome triggers sister chromatid separation and MPF degradation.

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    Separase

    A proteolytic enzyme that destroys cohesins, allowing sister chromatids to separate during anaphase.

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    Cohesins

    Proteins that hold sister chromatids together until separated by active separase.

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    p53 protein

    A protein that regulates the cell cycle and can induce arrest or apoptosis in response to DNA damage.

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    p21 protein

    A cyclin-dependent kinase inhibitor produced by activated p53 to halt the cell cycle in G1 phase.

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    Cyclin-dependent kinase inhibitors

    Proteins that regulate cell cycle progression by inhibiting cyclin-CDK complexes.

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    CIP/KIP proteins

    A subclass of CDKI that inhibit Cdk1, Cdk2, Cdk4, and Cdk6; includes p21, p27, and p57.

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    INK4 proteins

    A subclass of CDKI that specifically inhibits Cdk4 and Cdk6; examples include p15, p16, p18, and p19.

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    Apoptosis

    Process of programmed cell death that is energy-dependent and gene-activated, removing damaged cells.

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    Necrosis

    A passive form of cell death affecting groups of cells, often due to external damage, resulting in inflammation.

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    Diakinesis

    Final stage of prophase I in meiosis where chromosomes condense and become visible.

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    Metaphase I

    Bivalents align at the equatorial plane, with spindle fibers attaching to one chromatid.

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    Anaphase I

    Homologous chromosomes separate and move toward cell poles.

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    Telophase I

    Two daughter cells are formed with a haploid set of chromosomes; cell division occurs.

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    Meiosis II

    Follows meiosis I without an S phase, resulting in four haploid gametes from two daughter cells.

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    Prophase II

    Chromatin condenses, nuclear envelope disappears and spindle re-forms in two daughter cells.

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    Anaphase II

    Sister chromatids separate and move to opposite poles, becoming daughter chromosomes.

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    Telophase II

    Four haploid daughter nuclei form; cytokinesis follows.

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    Cell Cycle Checkpoints

    Stages that ensure conditions are right for cell division; decision points for continuing the cycle.

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    S Checkpoint

    Checks DNA for damage and verifies replication forks during S phase.

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    Study Notes

    Cell Cycle and Regulation

    • The cell cycle is a series of events that lead to a eukaryotic cell's division.
    • The cycle aims to accurately duplicate DNA and then segregate the duplicated DNA into genetically identical daughter cells, ensuring each cell receives a complete copy of the genome.

    Types of Cells

    • Diploid cells (2n) have a double set of chromosomes.
      • Somatic cells (all cells in the body) are diploid.
      • A human somatic cell contains 46 chromosomes (2n=46).
    • Haploid cells (1n) contain a single set of chromosomes.
      • Gametes (eggs and sperm) are haploid.
      • A human gamete contains 23 chromosomes (n=23).

    Types of Cell Division

    • Mitosis: Daughter cells receive the same number of chromosomes as the parent cell.
    • Meiosis: Daughter cells receive half the number of chromosomes as the parent cell.

    The Cell Cycle

    • The cell cycle is characterized by biochemical, physical, and structural changes.
    • Interphase: This phase makes up 95% of the cell cycle and includes:
      • G₁ phase (First gap): Cell growth phase, synthesis of structural and enzymatic proteins, increase in mitochondria and lysosomes, and increase in cell mass and volume.
      • S phase (Synthesis): DNA synthesis; each chromosome consists of two identical sister chromatids linked by cohesins.
      • G₂ phase (Second gap): More cell growth and organization for division, synthesis of proteins and lipids for cell membrane reconstruction.
    • Mitotic phase:
      • Mitosis (or meiosis), division of the cell's nucleus and DNA.
      • Cytokinesis: Division of the cytoplasm.
    • G₀ phase (resting phase): Cells do not divide and may stay in this phase indefinitely (e.g., nerve cells).

    Cell Cycle Duration

    • The duration of the cell cycle varies depending on the cell type.
      • Examples include fly embryos (8 minutes), frog embryos (30 minutes), mammalian intestinal epithelial cells (~12 hours), and mammalian fibroblasts in culture (~20 hours).

    Checkpoints

    • Checkpoints are stages in the cell cycle where both internal and external conditions are assessed.
    • Decisions are made about whether to proceed with cell division.
    • Specific checkpoints occur between G₁ and S, during S, between G₂ and M or during mitosis/meiosis.

    Cell Cycle Regulators

    • External regulators like mitogens (proteins or peptides) signal the cell for division via protein kinases.
    • Internal regulators like cyclins regulate and bind to protein kinases, and cyclin-dependent kinases (CDKs).
      • Cyclin-CDK complexes vary in activity, dependent on the cell cycle phase.
    • There are four main types of cyclins (D, E, A, and B).
    • The activities and concentrations of cyclins and CDKs regulate the cell progression through the different stages of the cell cycle.
    • APC/C (anaphase-promoting complex/cyclosome) degrades cyclins after a specific step in the cell cycle triggering the separation of sister chromatids and the end of the M phase, enabling the cell to enter the next phase.

    Apoptosis

    • Apoptosis is programmed cell death, an active process characteristically involving many genes and requiring an energy input (ATP).
    • Apoptosis is responsible for controlling the number and type of cells and removing damaged cells.
    • Apoptosis also ensures tissue homeostasis.

    Necrosis

    • Necrosis is the death of entire groups of cells, a random, passive process not needing energy.
    • Necrosis occurs due to physical or chemical external factors and leads to an inflammatory response.
    • Necrosis is characterized by cell swelling, loss of membrane contiguity, and the leakage of cell contents.

    Meiosis

    • Meiosis occurs in sex cells (germ cells).
    • It consists of two successive divisions of the diploid cell nucleus, producing four haploid cells.
    • The first division (meiosis I) is a reduction division and the second division (meiosis II) is compensatory.
    • Prophase I has 5 stages (leptotene, zygotene, pachytene, diplotene, diakinesis).
      • Chiasmata are formed between non-sister chromatids in homologous chromosomes.
      • Homologous recombination (crossing-over) occurs.
    • Metaphase I: Bivalents line up along the equatorial plane.
    • Anaphase I: Homologous chromosomes separate and migrate to opposite poles.
    • Telophase I: Two daughter cells are formed with half the number of chromosomes.

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    Description

    Test your knowledge on the stages of meiosis, particularly Prophase I. This quiz covers key events such as homologous recombination, the role of the synaptonemal complex, and differences between mitosis and meiosis. Delve into chromosome behavior and segregation errors during meiosis with this engaging quiz.

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