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Questions and Answers
During which stage of Prophase I does homologous recombination, or crossing-over, occur, leading to the exchange of DNA segments between non-sister chromatids?
During which stage of Prophase I does homologous recombination, or crossing-over, occur, leading to the exchange of DNA segments between non-sister chromatids?
What is the primary role of the synaptonemal complex during Zygotene?
What is the primary role of the synaptonemal complex during Zygotene?
In which stage of Prophase I do chiasmata become visible as homologous chromosomes begin to separate, but chromatid pairs remain connected?
In which stage of Prophase I do chiasmata become visible as homologous chromosomes begin to separate, but chromatid pairs remain connected?
If a cell in Pachytene has 3 bivalents, and each bivalent forms an average of 2 chiasmata, how many crossing-over events are likely to occur?
If a cell in Pachytene has 3 bivalents, and each bivalent forms an average of 2 chiasmata, how many crossing-over events are likely to occur?
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What is the fundamental difference between mitosis and meiosis in eukaryotic cells?
What is the fundamental difference between mitosis and meiosis in eukaryotic cells?
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Considering a human cell undergoing meiosis, what would be the outcome if a chromosome segregation error occurred during meiosis I, resulting in one daughter cell receiving both homologous chromosomes for chromosome 21?
Considering a human cell undergoing meiosis, what would be the outcome if a chromosome segregation error occurred during meiosis I, resulting in one daughter cell receiving both homologous chromosomes for chromosome 21?
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Which event is characteristic of the Leptotene stage of Prophase I?
Which event is characteristic of the Leptotene stage of Prophase I?
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Which is the correct pairing of cell type and number of chromosomes?
Which is the correct pairing of cell type and number of chromosomes?
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How does the chromosome number change during the transition from a diploid cell to a haploid cell?
How does the chromosome number change during the transition from a diploid cell to a haploid cell?
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If a diploid cell from an organism has 24 chromosomes, how many chromosomes would be present in a haploid cell from the same organism?
If a diploid cell from an organism has 24 chromosomes, how many chromosomes would be present in a haploid cell from the same organism?
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What is the primary role of the Cyclin A-Cdk2 complex during the S phase of the cell cycle?
What is the primary role of the Cyclin A-Cdk2 complex during the S phase of the cell cycle?
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How does the MPF complex contribute to the progression of a cell through the M phase?
How does the MPF complex contribute to the progression of a cell through the M phase?
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Which event is most critical in the early stages of the M phase and is directly influenced by the MPF complex?
Which event is most critical in the early stages of the M phase and is directly influenced by the MPF complex?
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What crucial mechanism ensures the activation of the MPF complex, facilitating a cell's entry into the M phase?
What crucial mechanism ensures the activation of the MPF complex, facilitating a cell's entry into the M phase?
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How does the MPF complex activation process amplify itself to ensure a robust transition into the M phase?
How does the MPF complex activation process amplify itself to ensure a robust transition into the M phase?
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What is the direct consequence of the APC/C complex ubiquitinating cyclin?
What is the direct consequence of the APC/C complex ubiquitinating cyclin?
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How does the activation of p53 protein contribute to cell cycle arrest in response to DNA damage?
How does the activation of p53 protein contribute to cell cycle arrest in response to DNA damage?
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What is the role of separase once it is released from the securin-separase complex?
What is the role of separase once it is released from the securin-separase complex?
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What is the immediate consequence of securin degradation by the APC/C complex?
What is the immediate consequence of securin degradation by the APC/C complex?
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Which of the following events marks the end of the M phase and allows new daughter cells to enter the G1 phase?
Which of the following events marks the end of the M phase and allows new daughter cells to enter the G1 phase?
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How do INK4 proteins inhibit the cell cycle progression?
How do INK4 proteins inhibit the cell cycle progression?
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What role does the protein p27 play in the cell cycle?
What role does the protein p27 play in the cell cycle?
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Under what circumstances would a cell typically undergo apoptosis?
Under what circumstances would a cell typically undergo apoptosis?
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How does necrosis differ fundamentally from apoptosis?
How does necrosis differ fundamentally from apoptosis?
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What is the immediate consequence of a cell undergoing necrosis?
What is the immediate consequence of a cell undergoing necrosis?
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During Diakinesis, which event is characteristically observed regarding the chiasmata?
During Diakinesis, which event is characteristically observed regarding the chiasmata?
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What is the crucial event that defines Anaphase I of meiosis?
What is the crucial event that defines Anaphase I of meiosis?
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What is the significance of chromosome decondensation during Telophase I?
What is the significance of chromosome decondensation during Telophase I?
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How does Meiosis II differ from Mitosis?
How does Meiosis II differ from Mitosis?
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During Metaphase II, what ensures proper chromosome segregation?
During Metaphase II, what ensures proper chromosome segregation?
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What is the immediate outcome of Anaphase II?
What is the immediate outcome of Anaphase II?
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What is the primary purpose of cell cycle checkpoints?
What is the primary purpose of cell cycle checkpoints?
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What cellular process is directly monitored at the S checkpoint?
What cellular process is directly monitored at the S checkpoint?
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How do cell cycle regulators typically exert their control?
How do cell cycle regulators typically exert their control?
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Consider a scenario where a cell has passed the G1 checkpoint but then encounters significant DNA damage during the S phase. What is the likely cellular response?
Consider a scenario where a cell has passed the G1 checkpoint but then encounters significant DNA damage during the S phase. What is the likely cellular response?
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Flashcards
Leptotene
Leptotene
Stage in Prophase I where chromatin condenses into chromosomes; each has two sister chromatids.
Zygotene
Zygotene
Stage in Prophase I where homologous chromosomes pair to form bivalents/tetrads and the synaptonemal complex forms.
Pachytene
Pachytene
Stage in Prophase I where chromosomes condense further and crossing-over occurs at chiasmata between non-sister chromatids.
Crossing-over
Crossing-over
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Diplotene
Diplotene
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Diploid cells
Diploid cells
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Haploid cells
Haploid cells
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Mitosis
Mitosis
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Meiosis
Meiosis
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Cell Cycle
Cell Cycle
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Cyclin A and Cdk2
Cyclin A and Cdk2
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S phase
S phase
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G2 phase
G2 phase
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M-phase Promoting Factor (MPF)
M-phase Promoting Factor (MPF)
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Positive-feedback loop in MPF activation
Positive-feedback loop in MPF activation
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APC/C
APC/C
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Separase
Separase
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Cohesins
Cohesins
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p53 protein
p53 protein
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p21 protein
p21 protein
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Cyclin-dependent kinase inhibitors
Cyclin-dependent kinase inhibitors
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CIP/KIP proteins
CIP/KIP proteins
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INK4 proteins
INK4 proteins
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Apoptosis
Apoptosis
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Necrosis
Necrosis
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Diakinesis
Diakinesis
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Metaphase I
Metaphase I
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Anaphase I
Anaphase I
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Telophase I
Telophase I
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Meiosis II
Meiosis II
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Prophase II
Prophase II
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Anaphase II
Anaphase II
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Telophase II
Telophase II
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Cell Cycle Checkpoints
Cell Cycle Checkpoints
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S Checkpoint
S Checkpoint
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Study Notes
Cell Cycle and Regulation
- The cell cycle is a series of events that lead to a eukaryotic cell's division.
- The cycle aims to accurately duplicate DNA and then segregate the duplicated DNA into genetically identical daughter cells, ensuring each cell receives a complete copy of the genome.
Types of Cells
- Diploid cells (2n) have a double set of chromosomes.
- Somatic cells (all cells in the body) are diploid.
- A human somatic cell contains 46 chromosomes (2n=46).
- Haploid cells (1n) contain a single set of chromosomes.
- Gametes (eggs and sperm) are haploid.
- A human gamete contains 23 chromosomes (n=23).
Types of Cell Division
- Mitosis: Daughter cells receive the same number of chromosomes as the parent cell.
- Meiosis: Daughter cells receive half the number of chromosomes as the parent cell.
The Cell Cycle
- The cell cycle is characterized by biochemical, physical, and structural changes.
- Interphase: This phase makes up 95% of the cell cycle and includes:
- G₁ phase (First gap): Cell growth phase, synthesis of structural and enzymatic proteins, increase in mitochondria and lysosomes, and increase in cell mass and volume.
- S phase (Synthesis): DNA synthesis; each chromosome consists of two identical sister chromatids linked by cohesins.
- G₂ phase (Second gap): More cell growth and organization for division, synthesis of proteins and lipids for cell membrane reconstruction.
- Mitotic phase:
- Mitosis (or meiosis), division of the cell's nucleus and DNA.
- Cytokinesis: Division of the cytoplasm.
- G₀ phase (resting phase): Cells do not divide and may stay in this phase indefinitely (e.g., nerve cells).
Cell Cycle Duration
- The duration of the cell cycle varies depending on the cell type.
- Examples include fly embryos (8 minutes), frog embryos (30 minutes), mammalian intestinal epithelial cells (~12 hours), and mammalian fibroblasts in culture (~20 hours).
Checkpoints
- Checkpoints are stages in the cell cycle where both internal and external conditions are assessed.
- Decisions are made about whether to proceed with cell division.
- Specific checkpoints occur between G₁ and S, during S, between G₂ and M or during mitosis/meiosis.
Cell Cycle Regulators
- External regulators like mitogens (proteins or peptides) signal the cell for division via protein kinases.
- Internal regulators like cyclins regulate and bind to protein kinases, and cyclin-dependent kinases (CDKs).
- Cyclin-CDK complexes vary in activity, dependent on the cell cycle phase.
- There are four main types of cyclins (D, E, A, and B).
- The activities and concentrations of cyclins and CDKs regulate the cell progression through the different stages of the cell cycle.
- APC/C (anaphase-promoting complex/cyclosome) degrades cyclins after a specific step in the cell cycle triggering the separation of sister chromatids and the end of the M phase, enabling the cell to enter the next phase.
Apoptosis
- Apoptosis is programmed cell death, an active process characteristically involving many genes and requiring an energy input (ATP).
- Apoptosis is responsible for controlling the number and type of cells and removing damaged cells.
- Apoptosis also ensures tissue homeostasis.
Necrosis
- Necrosis is the death of entire groups of cells, a random, passive process not needing energy.
- Necrosis occurs due to physical or chemical external factors and leads to an inflammatory response.
- Necrosis is characterized by cell swelling, loss of membrane contiguity, and the leakage of cell contents.
Meiosis
- Meiosis occurs in sex cells (germ cells).
- It consists of two successive divisions of the diploid cell nucleus, producing four haploid cells.
- The first division (meiosis I) is a reduction division and the second division (meiosis II) is compensatory.
- Prophase I has 5 stages (leptotene, zygotene, pachytene, diplotene, diakinesis).
- Chiasmata are formed between non-sister chromatids in homologous chromosomes.
- Homologous recombination (crossing-over) occurs.
- Metaphase I: Bivalents line up along the equatorial plane.
- Anaphase I: Homologous chromosomes separate and migrate to opposite poles.
- Telophase I: Two daughter cells are formed with half the number of chromosomes.
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Description
Test your knowledge on the stages of meiosis, particularly Prophase I. This quiz covers key events such as homologous recombination, the role of the synaptonemal complex, and differences between mitosis and meiosis. Delve into chromosome behavior and segregation errors during meiosis with this engaging quiz.