Biology Lab Questions Flashcards
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Questions and Answers

Chambers A and B are placed next to each other, but are separated by a film similar to a cell membrane. Chamber A contains 80% carbon dioxide and Chamber B contains 20% carbon dioxide. Which of the following will occur? (Select all that apply)

  • All of the carbon dioxide will move into Chamber B.
  • Some carbon dioxide will move from Chamber B to Chamber A. (correct)
  • Some carbon dioxide will move from Chamber A to Chamber B. (correct)
  • All of the carbon dioxide will move into Chamber A.
  • There will be no net movement of carbon dioxide molecules.
  • In simple diffusion, molecules move from an area of ____ concentration to an area of ____ concentration.

  • high; low (correct)
  • low; equal
  • low; high
  • equal; low
  • equal; high
  • What is the boiling point of water on the Celsius temperature scale?

    100 C

    What is the freezing point of water on the Celsius temperature scale?

    <p>0 C</p> Signup and view all the answers

    The liter is the metric base unit of measurement for what?

    <p>Volume</p> Signup and view all the answers

    What is the freezing point of water on the Fahrenheit temperature scale?

    <p>32 F</p> Signup and view all the answers

    The lens on the microscope that is primarily responsible for the magnification of the specimen is the _____ lens.

    <p>objective</p> Signup and view all the answers

    Which of the image quality factors is usually expressed in a number such as 40x?

    <p>magnification</p> Signup and view all the answers

    You have successfully focused on an object with the 10x and 40x objective lenses. You have rotated the 100x objective lens into position but are unable to obtain a sharp image by rotating the fine focus adjustment knob. Which of the following is most likely to correct this problem?

    <p>Add a drop of immersion oil before rotating the 100x lens into position.</p> Signup and view all the answers

    A change in what component of microscopy influences the apparent size of the objects being viewed?

    <p>magnification</p> Signup and view all the answers

    The objective lenses of the compound light microscope are attached to the _____

    <p>rotating nosepiece</p> Signup and view all the answers

    Using oil immersion is useful because

    <p>oil immersion increases the resolving power of the specimen.</p> Signup and view all the answers

    The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.

    <p>multiplying</p> Signup and view all the answers

    The _______ lenses further magnify the image produced by the objective lens.

    <p>ocular</p> Signup and view all the answers

    Which part of the microscope will be used first to adjust the focus when starting with the lowest power lens?

    <p>coarse focus</p> Signup and view all the answers

    The fine adjustment knob on the microscope

    <p>All of the answer choices are correct.</p> Signup and view all the answers

    Which of the following molecules is produced in the oxidation of NADH during fermentation?

    <p>lactate</p> Signup and view all the answers

    Which of the following processes will occur in the presence or absence of oxygen?

    <p>glycolysis</p> Signup and view all the answers

    In the yeast fermentation experiment, what is being produced to cause the air bubble at the top of the tube to increase over time?

    <p>CO2</p> Signup and view all the answers

    Which of the following substrates is fermented most easily by yeast?

    <p>glucose</p> Signup and view all the answers

    A device that can be used to measure the rate of exchange of oxygen and carbon dioxide during energy-acquiring pathways is called a

    <p>respirometer</p> Signup and view all the answers

    During the experiment measuring energy production in plants, the highest rate of cellular respiration occurred in

    <p>the tube containing germinating seeds.</p> Signup and view all the answers

    During the soybean experiment, the nongerminating soybeans consumed around 0.02 mL/min of oxygen. What does this tell you about the cellular respiration of the nongerminating soybeans?

    <p>A little cellular respiration was taking place but not enough for growth.</p> Signup and view all the answers

    Study Notes

    Gas Diffusion and Concentration

    • Chambers A and B separated by a membrane with 80% CO2 in A and 20% CO2 in B.
    • Some carbon dioxide will move from chamber B to chamber A due to concentration gradient.

    Simple Diffusion

    • In simple diffusion, molecules move from high concentration to low concentration.

    Boiling and Freezing Points of Water

    • Boiling point of water is 100°C.
    • Freezing point of water is 0°C.

    Measurement Units

    • The liter is the metric base unit for measuring volume.

    Freezing Points in Fahrenheit

    • Freezing point of water is 32°F.

    Microscopy Fundamentals

    • The objective lens is responsible for magnification in microscopy.
    • Image quality is often expressed in magnification (e.g., 40x).

    Focusing Techniques

    • Inability to obtain a sharp image with the 100x objective lens may be corrected by applying immersion oil.
    • Total magnification is calculated by multiplying the magnification powers of ocular and objective lenses.
    • Coarse focus is used first when adjusting focus with the lowest power lens.

    Oil Immersion Benefits

    • Oil immersion increases the resolving power of the specimen.

    Additional Lens Functions

    • Ocular lenses further magnify the image produced by the objective lens.
    • Fine adjustment knob fine-tunes the focus of the microscope.

    Fermentation and Metabolism

    • Lactate is produced in the oxidation of NADH during fermentation.
    • Glycolysis occurs in the presence or absence of oxygen.

    Yeast Fermentation Experiment

    • Carbon dioxide (CO2) production causes air bubbles to increase in the fermentation tube.
    • Glucose is the substrate that is most easily fermented by yeast.

    Measuring Respiration

    • A respirometer measures the exchange rate of oxygen and carbon dioxide in energy-acquiring pathways.
    • Highest rate of cellular respiration observed in tubes with germinating seeds.

    Cellular Respiration Observations

    • Non-germinating soybeans consumed approximately 0.02 mL/min of oxygen, indicating a minimal level of cellular respiration.

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    Test your understanding of gas exchange principles in cellular environments with these flashcards. The quiz covers various scenarios involving carbon dioxide movement between chambers separated by a membrane. Perfect for biology students needing a quick review.

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