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Questions and Answers
What is the most common type of microscope?
What is the most common type of microscope?
- Electron microscope
- Phase contrast microscope
- Light microscope (correct)
- Fluorescence microscope
What is the main advantage of phase contrast microscope?
What is the main advantage of phase contrast microscope?
- It allows for viewing of living cells and cell division (correct)
- It produces high-quality images
- It is extremely simple to use
- It is inexpensive to set up
What type of microscope is used to examine blood cells?
What type of microscope is used to examine blood cells?
- Electron microscope
- Fluorescence microscope
- Stereo microscope
- Dark field microscope (correct)
What is the name of the microscope that uses a special condenser and objective with a diaphragm to scatter light?
What is the name of the microscope that uses a special condenser and objective with a diaphragm to scatter light?
What type of microscope is used to study living cells without killing them by staining?
What type of microscope is used to study living cells without killing them by staining?
What is the main difference between a simple microscope and a compound microscope?
What is the main difference between a simple microscope and a compound microscope?
What type of microscope is used to view the structure of cells clearly?
What type of microscope is used to view the structure of cells clearly?
What is the purpose of a microscope in a medical laboratory?
What is the purpose of a microscope in a medical laboratory?
What is the purpose of fixation in sample preparation?
What is the purpose of fixation in sample preparation?
What is the main difference between TEM and SEM?
What is the main difference between TEM and SEM?
What is the purpose of sputter coating in sample preparation?
What is the purpose of sputter coating in sample preparation?
What is a disadvantage of using an electron microscope?
What is a disadvantage of using an electron microscope?
What is the primary function of a stereo microscope?
What is the primary function of a stereo microscope?
What is the purpose of cryofixation in sample preparation?
What is the purpose of cryofixation in sample preparation?
What is a digital microscope?
What is a digital microscope?
What is the most frequently used chemical in chemical fixation?
What is the most frequently used chemical in chemical fixation?
What is a disadvantage of the microscope type discussed by Hayder Ahmed?
What is a disadvantage of the microscope type discussed by Hayder Ahmed?
What type of light is used in fluorescence microscopy?
What type of light is used in fluorescence microscopy?
What is the purpose of the special optical filters in fluorescence microscopy?
What is the purpose of the special optical filters in fluorescence microscopy?
What is the advantage of electron microscopes over light microscopes?
What is the advantage of electron microscopes over light microscopes?
What lenses are used in electron microscopes?
What lenses are used in electron microscopes?
Why do electron microscopes have a greater resolving power than light microscopes?
Why do electron microscopes have a greater resolving power than light microscopes?
What is the maximum magnification possible with modern electron microscopes?
What is the maximum magnification possible with modern electron microscopes?
What is the function of the fluorescent dye in fluorescence microscopy?
What is the function of the fluorescent dye in fluorescence microscopy?
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Study Notes
Types of Microscopes
- Light microscope: uses light to pass through the sample to produce an image
- Simple microscope: oldest design, magnifies small samples
- Compound microscope: commonly used in medical laboratories
Phase Contrast Microscope
- Enhances contrast of transparent and colorless objects by influencing light's optical path
- Allows for examination of living cells and cell division without staining
Dark Field Microscope
- Uses a special condenser and objective with a diaphragm to scatter light from cells
- Cells appear bright on a dark background
- Applications: viewing blood cells, bacteria, and cell structure
- Advantages: simple to use, inexpensive
- Disadvantages: limited to black and white colors
Fluorescence Microscope
- Uses fluorescence and phosphorescence to study properties of substances
- Uses ultra-violet light to activate specific parts of the sample
- Fluorochrome dye is used to make the sample glow
- Special optical filters absorb all wavelengths except the dye used
Electron Microscope
- Uses a beam of electrons to create an image of the specimen
- Capable of higher magnifications and greater resolving power than light microscopes
- Types:
- Transmission Electron Microscope (TEM): electron beam passes through the sample
- Scanning Electron Microscope (SEM): electron beam scans the surface of the sample
- Disadvantages: expensive, difficult to use
Sample Preparation for Electron Microscope
- Fixation: stabilizes the cell using chemical or cryofixation methods
- Dehydration
- Embedding
- Sectioning
- Staining
- Freezing
- Sputter Coating
Stereo Microscope (Dissecting Microscope)
- Designed for low magnification to observe the sample using light
- Used for examining insects and anatomical dissection
Digital Microscope
- Traditional microscope that uses a digital camera to output an image to a monitor
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