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Questions and Answers
What is the primary factor contributing to the rapid evolution of prokaryotes?
What is the primary factor contributing to the rapid evolution of prokaryotes?
Which process involves the transfer of genetic material between prokaryotic cells in direct contact?
Which process involves the transfer of genetic material between prokaryotic cells in direct contact?
What is the term for the combination of DNA from two sources?
What is the term for the combination of DNA from two sources?
Which type of prokaryote obtains energy from light?
Which type of prokaryote obtains energy from light?
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What is the term for the movement of genes among individuals from different species?
What is the term for the movement of genes among individuals from different species?
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Which process involves the uptake and incorporation of foreign DNA by prokaryotic cells from their surroundings?
Which process involves the uptake and incorporation of foreign DNA by prokaryotic cells from their surroundings?
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What is the term for the exchange of DNA between bacteria mediated by bacteriophages?
What is the term for the exchange of DNA between bacteria mediated by bacteriophages?
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Which type of prokaryote requires an organic nutrient as a carbon source?
Which type of prokaryote requires an organic nutrient as a carbon source?
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What is the main component of Gram-positive bacteria cell walls?
What is the main component of Gram-positive bacteria cell walls?
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What is the result of Gram staining in Gram-negative bacteria?
What is the result of Gram staining in Gram-negative bacteria?
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What is the function of the peptidoglycan layer in Gram-positive bacteria?
What is the function of the peptidoglycan layer in Gram-positive bacteria?
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Which of the following bacteria is an example of Gram-positive bacteria?
Which of the following bacteria is an example of Gram-positive bacteria?
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What is the composition of the outer membrane in Gram-negative bacteria?
What is the composition of the outer membrane in Gram-negative bacteria?
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What is the difference between Gram-positive and Gram-negative bacteria in terms of resistance to lysozyme?
What is the difference between Gram-positive and Gram-negative bacteria in terms of resistance to lysozyme?
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What is the space between the inner and outer membrane in Gram-negative bacteria called?
What is the space between the inner and outer membrane in Gram-negative bacteria called?
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What is the result of Gram staining in Gram-positive bacteria?
What is the result of Gram staining in Gram-positive bacteria?
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What is the alternative structure used by Flexibacter polymorphus instead of flagella?
What is the alternative structure used by Flexibacter polymorphus instead of flagella?
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Which of the following phospholipids is NOT present in the plasma membrane of prokaryotic cells?
Which of the following phospholipids is NOT present in the plasma membrane of prokaryotic cells?
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What is the function of mesosomes in some prokaryotes?
What is the function of mesosomes in some prokaryotes?
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Which of the following is a characteristic of the plasma membrane of prokaryotic cells?
Which of the following is a characteristic of the plasma membrane of prokaryotic cells?
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What is the main difference between prokaryotic and eukaryotic cells in terms of internal organization?
What is the main difference between prokaryotic and eukaryotic cells in terms of internal organization?
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What is the movement of genes among individuals from different species called?
What is the movement of genes among individuals from different species called?
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Which of the following lipids is NOT present in the plasma membrane of hepatocytes?
Which of the following lipids is NOT present in the plasma membrane of hepatocytes?
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What is the primary function of the plasma membrane in cells?
What is the primary function of the plasma membrane in cells?
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Which bacterium is a potential weapon of bioterrorism?
Which bacterium is a potential weapon of bioterrorism?
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Which of the following is a type of lipid found in the plasma membrane of myelin?
Which of the following is a type of lipid found in the plasma membrane of myelin?
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What is the causative agent of pneumococcal meningitis?
What is the causative agent of pneumococcal meningitis?
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What is the name of the methicillin-resistant strain of Staphylococcus aureus?
What is the name of the methicillin-resistant strain of Staphylococcus aureus?
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Which bacterium causes gonococcal urethritis?
Which bacterium causes gonococcal urethritis?
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What is the characteristic of Streptococcal colonies?
What is the characteristic of Streptococcal colonies?
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Which disease is characterized by chronic cough with blood-containing sputum?
Which disease is characterized by chronic cough with blood-containing sputum?
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What is the bacterium that causes leprosy?
What is the bacterium that causes leprosy?
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Which type of bacteria lacks a cell wall?
Which type of bacteria lacks a cell wall?
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What is the function of botulinum toxin?
What is the function of botulinum toxin?
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Which bacterium is the source of many antibiotics?
Which bacterium is the source of many antibiotics?
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What is the cause of tetanus?
What is the cause of tetanus?
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What is the result of respiratory muscle failure due to botulism?
What is the result of respiratory muscle failure due to botulism?
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Which type of bacteria is Gram-Positive?
Which type of bacteria is Gram-Positive?
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What is the name of the disease caused by Bacillus anthracis?
What is the name of the disease caused by Bacillus anthracis?
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What is the result of improper canning of food contaminated with Clostridium botulinum?
What is the result of improper canning of food contaminated with Clostridium botulinum?
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What is the primary function of prokaryotic ribosomes?
What is the primary function of prokaryotic ribosomes?
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What is the characteristic of prokaryotic chromosomes?
What is the characteristic of prokaryotic chromosomes?
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What is the function of plasmids in prokaryotes?
What is the function of plasmids in prokaryotes?
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What is the name of the region that contains the prokaryotic chromosome?
What is the name of the region that contains the prokaryotic chromosome?
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What is the term for the ability of some prokaryotes to form endospores?
What is the term for the ability of some prokaryotes to form endospores?
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What is the difference between prokaryotic and eukaryotic ribosomes?
What is the difference between prokaryotic and eukaryotic ribosomes?
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What is the function of mesosomes in some prokaryotes?
What is the function of mesosomes in some prokaryotes?
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What is the shape of prokaryotic chromosomes?
What is the shape of prokaryotic chromosomes?
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What is the primary mechanism of transmission of tetanus?
What is the primary mechanism of transmission of tetanus?
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What is the characteristic feature of Corynebacterium diptheriae?
What is the characteristic feature of Corynebacterium diptheriae?
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What is the unique feature of the cell wall of Mycobacteria?
What is the unique feature of the cell wall of Mycobacteria?
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What is the primary function of diphtheria toxin?
What is the primary function of diphtheria toxin?
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What is the characteristic symptom of tetanus?
What is the characteristic symptom of tetanus?
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What is the distinguishing feature of Mycoplasmas?
What is the distinguishing feature of Mycoplasmas?
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Which of the following bacteria is associated with non-gonococcal urethritis?
Which of the following bacteria is associated with non-gonococcal urethritis?
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Why are Mycobacteria classified as acid-fast Gram-positive bacteria?
Why are Mycobacteria classified as acid-fast Gram-positive bacteria?
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What is the common characteristic among the organisms classified under Domain Bacteria and Domain Archaea?
What is the common characteristic among the organisms classified under Domain Bacteria and Domain Archaea?
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What is the characteristic that defines the members of Kingdom Fungi?
What is the characteristic that defines the members of Kingdom Fungi?
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Which of the following kingdoms consists of multicellular eukaryotes that carry out photosynthesis?
Which of the following kingdoms consists of multicellular eukaryotes that carry out photosynthesis?
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What is the characteristic of the organisms classified under Domain Eukarya?
What is the characteristic of the organisms classified under Domain Eukarya?
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In which type of environment do Domain Archaea organisms typically live?
In which type of environment do Domain Archaea organisms typically live?
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What is the number of kingdoms that Domain Eukarya is divided into?
What is the number of kingdoms that Domain Eukarya is divided into?
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What is the characteristic of Protists?
What is the characteristic of Protists?
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Which of the following is NOT a characteristic of Domain Bacteria?
Which of the following is NOT a characteristic of Domain Bacteria?
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What is the characteristic of endospores that allows them to survive in harsh environments?
What is the characteristic of endospores that allows them to survive in harsh environments?
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What is the main advantage of prokaryotes' short generation times?
What is the main advantage of prokaryotes' short generation times?
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What is the result of the process of binary fission in prokaryotes?
What is the result of the process of binary fission in prokaryotes?
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Which bacterium is an example of a sporogenic bacterium?
Which bacterium is an example of a sporogenic bacterium?
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What is the function of the cortex in endospore structure?
What is the function of the cortex in endospore structure?
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What is the characteristic of prokaryotic reproduction that allows for rapid evolution?
What is the characteristic of prokaryotic reproduction that allows for rapid evolution?
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What is the result of cell plate formation in prokaryotic cell division?
What is the result of cell plate formation in prokaryotic cell division?
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What is the term for the process of prokaryotic cell division that results in two identical daughter cells?
What is the term for the process of prokaryotic cell division that results in two identical daughter cells?
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What is the primary function of the capsule in some prokaryotes?
What is the primary function of the capsule in some prokaryotes?
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Which bacterium is used in gene cloning to produce transgenic plants?
Which bacterium is used in gene cloning to produce transgenic plants?
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What is the disease caused by Vibrio cholerae?
What is the disease caused by Vibrio cholerae?
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What is the bacterium that causes Syphilis?
What is the bacterium that causes Syphilis?
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Which bacterium can produce vitamins, antibiotics, and hormones?
Which bacterium can produce vitamins, antibiotics, and hormones?
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What is the disease caused by Neisseria meningitidis?
What is the disease caused by Neisseria meningitidis?
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What is the function of flagella in some prokaryotes?
What is the function of flagella in some prokaryotes?
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Which bacterium causes Gastritis, stomach ulcers, and gastric cancer?
Which bacterium causes Gastritis, stomach ulcers, and gastric cancer?
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What is the characteristic of prokaryotic ribosomes that allows some antibiotics to target them without affecting human ribosomes?
What is the characteristic of prokaryotic ribosomes that allows some antibiotics to target them without affecting human ribosomes?
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What is the primary function of thylakoid membranes in photosynthetic prokaryotes?
What is the primary function of thylakoid membranes in photosynthetic prokaryotes?
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What is the term for the viruses that infect bacteria and have a DNA genome?
What is the term for the viruses that infect bacteria and have a DNA genome?
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What is the characteristic of prokaryotic chromosomes that allows them to fit within the nucleoid region?
What is the characteristic of prokaryotic chromosomes that allows them to fit within the nucleoid region?
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What is the function of polysomes in prokaryotes?
What is the function of polysomes in prokaryotes?
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What is the characteristic of endospores that allows them to survive in harsh environments?
What is the characteristic of endospores that allows them to survive in harsh environments?
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What is the primary function of mesosomes in some prokaryotes?
What is the primary function of mesosomes in some prokaryotes?
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What is the characteristic of the plasma membrane in prokaryotic cells?
What is the characteristic of the plasma membrane in prokaryotic cells?
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What is the alternative structure used by Flexibacter polymorphus instead of flagella?
What is the alternative structure used by Flexibacter polymorphus instead of flagella?
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Which of the following lipids is present in the plasma membrane of prokaryotic cells?
Which of the following lipids is present in the plasma membrane of prokaryotic cells?
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What is the characteristic of prokaryotic cells in terms of compartmentalization?
What is the characteristic of prokaryotic cells in terms of compartmentalization?
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What is the function of the plasma membrane in cells?
What is the function of the plasma membrane in cells?
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Which of the following bacteria causes syphilis?
Which of the following bacteria causes syphilis?
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What is unique about cyanobacteria?
What is unique about cyanobacteria?
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What is the result of scarring of the fallopian tubes due to Chlamydia trachomatis infection?
What is the result of scarring of the fallopian tubes due to Chlamydia trachomatis infection?
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Which of the following Gram-positive bacteria causes diphtheria?
Which of the following Gram-positive bacteria causes diphtheria?
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What is the mode of transmission of Leptospira?
What is the mode of transmission of Leptospira?
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Which of the following bacteria is associated with non-gonococcal urethritis?
Which of the following bacteria is associated with non-gonococcal urethritis?
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What is the main characteristic of micro-organisms?
What is the main characteristic of micro-organisms?
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Which of the following is NOT a category of micro-organisms?
Which of the following is NOT a category of micro-organisms?
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What was the discovery of Alexander Fleming in 1929?
What was the discovery of Alexander Fleming in 1929?
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What is the primary characteristic of pathogenic bacteria species?
What is the primary characteristic of pathogenic bacteria species?
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What is the name of the disease caused by the Yersinia pestis bacterium?
What is the name of the disease caused by the Yersinia pestis bacterium?
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What is the primary function of Staphylococcus aureus?
What is the primary function of Staphylococcus aureus?
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What is the term for the 40-day duration that Venetian ships coming from countries with active plague epidemics remained under quarantine in the sea?
What is the term for the 40-day duration that Venetian ships coming from countries with active plague epidemics remained under quarantine in the sea?
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What is the primary characteristic of Mycobacterium tuberculosis?
What is the primary characteristic of Mycobacterium tuberculosis?
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What is the primary function of Neisseria gonorrhoeae?
What is the primary function of Neisseria gonorrhoeae?
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What is the characteristic of prokaryotes that allows them to thrive almost everywhere?
What is the characteristic of prokaryotes that allows them to thrive almost everywhere?
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What is the name of the domain that includes non-pathogenic bacteria that perform photosynthesis?
What is the name of the domain that includes non-pathogenic bacteria that perform photosynthesis?
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What is the primary characteristic of Horizontal gene transfer?
What is the primary characteristic of Horizontal gene transfer?
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What is the primary function of Bacillus cereus?
What is the primary function of Bacillus cereus?
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What is the system of naming living organisms invented by Carolus Linnaeus?
What is the system of naming living organisms invented by Carolus Linnaeus?
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What is the shape of prokaryotic cells?
What is the shape of prokaryotic cells?
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What is the name of the disease caused by the HIV virus?
What is the name of the disease caused by the HIV virus?
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What is a characteristic of Domain Eukarya?
What is a characteristic of Domain Eukarya?
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In which type of environment are Archaea typically found?
In which type of environment are Archaea typically found?
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What is the basis for defining Kingdom Fungi?
What is the basis for defining Kingdom Fungi?
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What is a characteristic of protists?
What is a characteristic of protists?
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How many kingdoms are included in Domain Eukarya?
How many kingdoms are included in Domain Eukarya?
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What is the characteristic of bacteria that allows them to be divided into multiple kingdoms?
What is the characteristic of bacteria that allows them to be divided into multiple kingdoms?
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What is the main component of the cell wall in Gram-positive bacteria?
What is the main component of the cell wall in Gram-positive bacteria?
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What is the function of the peptidoglycan layer in Gram-positive bacteria?
What is the function of the peptidoglycan layer in Gram-positive bacteria?
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Which of the following is an example of Gram-negative bacteria?
Which of the following is an example of Gram-negative bacteria?
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What is the function of the outer lipopolysaccharide layer in Gram-negative bacteria?
What is the function of the outer lipopolysaccharide layer in Gram-negative bacteria?
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What is the result of Gram-staining in Gram-positive bacteria?
What is the result of Gram-staining in Gram-positive bacteria?
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What is the difference between the cell wall structure of Gram-positive and Gram-negative bacteria?
What is the difference between the cell wall structure of Gram-positive and Gram-negative bacteria?
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What is the characteristic of archaea that distinguishes them from bacteria?
What is the characteristic of archaea that distinguishes them from bacteria?
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Which type of prokaryotes are poisoned by oxygen and use fermentation or anaerobic respiration?
Which type of prokaryotes are poisoned by oxygen and use fermentation or anaerobic respiration?
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What is the term for the process of converting atmospheric nitrogen (N2) to ammonia (NH3) by some prokaryotes?
What is the term for the process of converting atmospheric nitrogen (N2) to ammonia (NH3) by some prokaryotes?
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Which method has been used to investigate the phylogeny of prokaryotes?
Which method has been used to investigate the phylogeny of prokaryotes?
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What is the environment in which extreme thermophiles thrive?
What is the environment in which extreme thermophiles thrive?
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What is the term for the study of evolutionary relationships among groups of organisms using molecular sequencing data and morphological data?
What is the term for the study of evolutionary relationships among groups of organisms using molecular sequencing data and morphological data?
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What is the role of prokaryotes in the biosphere with regard to chemical elements?
What is the role of prokaryotes in the biosphere with regard to chemical elements?
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What is the type of symbiotic relationship where one organism benefits while the other is not affected?
What is the type of symbiotic relationship where one organism benefits while the other is not affected?
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What is the primary mechanism by which pathogenic prokaryotes cause disease?
What is the primary mechanism by which pathogenic prokaryotes cause disease?
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What is the term for the ecological relationship where two species live in close contact?
What is the term for the ecological relationship where two species live in close contact?
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What is the role of beneficial prokaryotes in the human body?
What is the role of beneficial prokaryotes in the human body?
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What is the process by which genes associated with virulence can be spread among bacteria?
What is the process by which genes associated with virulence can be spread among bacteria?
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What is the term for the 40-day duration that Venetian ships coming from countries with active plague epidemics remained under quarantine in the sea before they approached Venice port?
What is the term for the 40-day duration that Venetian ships coming from countries with active plague epidemics remained under quarantine in the sea before they approached Venice port?
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Which of the following micro-organisms are eukaryotic?
Which of the following micro-organisms are eukaryotic?
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What is the main advantage of micro-organisms' ability to replicate quickly?
What is the main advantage of micro-organisms' ability to replicate quickly?
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Which of the following pandemics was caused by the Yersinia pestis bacterium?
Which of the following pandemics was caused by the Yersinia pestis bacterium?
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What is the term for the classification of living organisms?
What is the term for the classification of living organisms?
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Which of the following is NOT a characteristic of prokaryotes?
Which of the following is NOT a characteristic of prokaryotes?
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What is the term for the system of naming living organisms invented by Carolus Linnaeus?
What is the term for the system of naming living organisms invented by Carolus Linnaeus?
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Which of the following is a type of micro-organism that is not cellular?
Which of the following is a type of micro-organism that is not cellular?
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What is the term for the ability of some prokaryotes to form endospores?
What is the term for the ability of some prokaryotes to form endospores?
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Which of the following is a characteristic of Domain Archaea?
Which of the following is a characteristic of Domain Archaea?
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Study Notes
Prokaryotic Cell Structure
- Prokaryotic cells have a cell wall that provides protection and maintenance of cell shape
- The cell wall is composed of peptidoglycan (Gram-positive) or peptidoglycan and lipopolysaccharides (Gram-negative)
Gram-Positive and Gram-Negative Bacteria
- Gram-positive bacteria:
- Cell wall composed of peptidoglycan
- Absorb crystal violet and appear purple
- Examples: Staphylococcus, Streptococcus, Micrococcus
- Gram-negative bacteria:
- Cell wall composed of peptidoglycan and lipopolysaccharides
- Do not absorb crystal violet and appear pink
- Examples: Escherichia coli, Shigella, Salmonella
Plasma Membrane
- Composed of phospholipids and proteins
- Selectively permeable (semi-permeable)
- Lipid composition varies between different membranes
Internal Organization and DNA
- Prokaryotic cells lack complex compartmentalization
- No membrane-bound nucleus or membrane-bound organelles
- Mesosomes: infoldings of the plasma membrane that perform metabolic functions
- Prokaryotic chromosome: a double-stranded circular DNA molecule
- Smaller than eukaryotic genome
- Absence of histones
- Supercoiled to fit the nucleoid region
Reproduction and Adaptation
- Prokaryotes reproduce quickly by binary fission
- Short generation times allow for rapid evolution
- Adaptation: development of antibiotic resistance
Pathogenic Bacteria
- Examples of pathogenic bacteria: Neisseria meningitidis, Neisseria gonorrhoeae, Escherichia coli, Salmonella, Legionella, Vibrio cholerae
- Causes of diseases: meningococcal meningitis, gonococcal urethritis, opportunistic infections, legionellosis, cholera
Prokaryotic Ribosomes
- Function: protein synthesis
- Consist of different subunits than eukaryotes
- Some antibiotics inhibit bacterial ribosomes without inhibiting human ribosomes
Nucleoid
- Region that contains the prokaryotic chromosome
- Not surrounded by a nuclear membrane
Endospores
- Formed under harsh conditions
- Metabolically inactive
- Remain viable in harsh conditions for years
- Resistant to temperature, dryness, UV light, enzymes, chemicals, and drugs
Sporogenic Bacteria
- Examples: Bacillus, Clostridium
- Ability to form endospores
Prokaryotic Cell Division
- Binary fission
- Short generation times
- Adaptation: development of antibiotic resistance### Horizontal Gene Transfer and Pathogenic Bacteria
- Horizontal gene transfer: movement of genes among individuals from different species
- Pathogenic bacteria:
- Examples: Bacillus anthracis, Staphylococcus aureus, Streptococcus pneumoniae, Bacillus cereus, Neisseria meningitidis, Neisseria gonorrhoeae, Escherichia coli, Mycobacterium tuberculosis, Mycobacterium leprae
- Can cause diseases such as toxic shock syndrome, food poisoning, pneumonia, meningitis, and tuberculosis
Prokaryotes and Microorganisms
- Prokaryotes:
- Unicellular microorganisms
- Consist of 1 prokaryotic cell
- Replicate faster than eukaryotes
- Smaller in size than eukaryotic cells
- Divided into 2 domains: Bacteria and Archaea
- Can thrive in extreme conditions
- Microorganisms:
- Microscopic organisms consisting of 1 cell (unicellular) or a group of cells (multicellular)
- Categories: Bacteria, Archaea, Protists, Fungi, and Viruses
Historical Evidence and Pandemics
- Historical evidence for the existence of microorganisms:
- Pathogenic microorganisms cause infectious diseases
- Examples: plague, cholera, and smallpox
- Pandemics:
- Viral and bacterial outbreaks throughout history
- Examples: Antonine plague, Justinian plague, Black Death, and COVID-19
Prokaryote Characteristics and Taxonomy
- Prokaryote characteristics:
- Smaller in size than eukaryotic cells
- Variable morphology
- Can thrive in extreme conditions
- Taxonomy:
- Classification of living organisms
- Species, Genus, Family, Order, Class, Phylum, Kingdom, and Domain
- Nomenclature of microorganisms: binomial system (e.g., Staphylococcus aureus)
Bacteria and Archaea
- Bacteria:
- 2 major categories: Eubacteria and Cyanobacteria
- Eubacteria: includes pathogenic bacteria
- Cyanobacteria: non-pathogenic, photosynthetic, and produce oxygen
- Archaea:
- Live in extreme conditions
- Examples: Halophiles, Methanogens, and Thermoacidophiles
- Initially named Archaebacteria due to morphological similarities with Bacteria
Structural and Functional Properties
- Prokaryotic cell size: 1-10 µm
- Variable morphology: spherical, rod-shaped, and spiral shapes
- Oxygen metabolism: obligate aerobes, obligate anaerobes, and facultative anaerobes
- Nitrogen metabolism: nitrogen fixation, conversion of nitrogen into ammonia
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Description
Test your knowledge of Gram-positive and Gram-negative bacteria, their cell walls, and the Gram staining process. Learn about the differences in their peptidoglycan and lipopolysaccharide composition. Identify the characteristics of each type of bacteria and how they react to crystal violet.