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Questions and Answers

What is the role of the independent variable in an experiment?

  • It remains constant throughout the experiment.
  • It is the factor that is manipulated by the researcher. (correct)
  • It is the same as the control group.
  • It is measured as a result of changes.

What distinguishes qualitative measurements from quantitative measurements?

  • Qualitative measurements cannot be tested, while quantitative can.
  • Qualitative measurements are numerical, while quantitative are descriptive.
  • Qualitative measurements involve colors and shapes, while quantitative involve counts and sizes. (correct)
  • Qualitative measurements are always subjective, whereas quantitative measurements are objective.

Which of the following statements is true regarding a null hypothesis?

  • It proposes a significant effect or relationship.
  • It assumes that no effect exists in the population. (correct)
  • It is always accepted if the research hypothesis is proven.
  • It usually represents a specific outcome predicted by observation.

In the context of cell theory, which statement is NOT a main component?

<p>Cells are the smallest particle that cannot be divided further. (A)</p> Signup and view all the answers

Which characteristic of the periodic table is indicated by its rows?

<p>Elements in a row share the same valence electrons. (A)</p> Signup and view all the answers

Which of the following describes a component that all viruses possess?

<p>A protein coat (capsid) (D)</p> Signup and view all the answers

Which of the following processes is categorized as exergonic?

<p>Cellular respiration (A)</p> Signup and view all the answers

How does the lytic cycle of bacteriophages primarily differ from the lysogenic cycle?

<p>The lytic cycle destroys the host cell immediately (C)</p> Signup and view all the answers

What is the primary role of intracellular receptors in cell signaling?

<p>To transduce signals received from outside the cell (D)</p> Signup and view all the answers

Which of the following statements about enzymes is accurate?

<p>Enzymes are biological catalysts that lower activation energy (C)</p> Signup and view all the answers

What is one reason why aerobic respiration is considered more efficient than anaerobic respiration?

<p>Aerobic respiration yields more ATP from glucose (A)</p> Signup and view all the answers

What is meant by denaturation of enzymes?

<p>Enzymes permanently losing their three-dimensional structure (D)</p> Signup and view all the answers

Which category of membrane proteins serve as receptors during cell signaling?

<p>Receptor proteins (A)</p> Signup and view all the answers

In which location does glycolysis occur within a eukaryotic cell?

<p>Cytoplasm (D)</p> Signup and view all the answers

Which of the following best describes the Light Independent Reactions in photosynthesis?

<p>They convert carbon dioxide into glucose (B)</p> Signup and view all the answers

What type of macromolecule do phospholipids belong to?

<p>Lipids (A)</p> Signup and view all the answers

What is a primary difference between DNA and RNA?

<p>RNA is usually single-stranded, while DNA is double-stranded. (B)</p> Signup and view all the answers

Which factor can affect the stability of protein folding?

<p>pH levels of the environment (C)</p> Signup and view all the answers

What type of transport requires energy and involves moving substances against their concentration gradient?

<p>Active transport (C)</p> Signup and view all the answers

Which of the following correctly describes a characteristic of eukaryotic cells?

<p>They contain membrane-bound organelles. (B)</p> Signup and view all the answers

What term is used to describe the process of a protein losing its structure and function due to environmental conditions?

<p>Denaturation (D)</p> Signup and view all the answers

Which type of junction connects plant cells and allows for intercellular communication?

<p>Plasmodesmata (A)</p> Signup and view all the answers

Which membrane protein is only temporarily attached to the membrane surface?

<p>Peripheral protein (C)</p> Signup and view all the answers

What process describes the movement of water across a semipermeable membrane?

<p>Osmosis (C)</p> Signup and view all the answers

What component of biological membranes plays a role in cell recognition?

<p>Glycoproteins (A)</p> Signup and view all the answers

What occurs if the centromeres do not attach to the spindle fibers during cell division?

<p>Chromosomes will not segregate correctly. (B)</p> Signup and view all the answers

Which of the following describes the Law of Segregation?

<p>Two alleles for a trait separate during the formation of gametes. (C)</p> Signup and view all the answers

What is the result of nondisjunction during meiosis?

<p>An organism will have an extra or missing chromosome. (A)</p> Signup and view all the answers

Which of the following best defines polyploidy?

<p>Having more than two complete sets of chromosomes. (C)</p> Signup and view all the answers

What are polygenic traits characterized by?

<p>Exhibit continuous variation. (B)</p> Signup and view all the answers

Which genetic disorder is an example of aneuploidy?

<p>Down syndrome (A)</p> Signup and view all the answers

What does incomplete dominance result in?

<p>A blending of traits is seen in the offspring. (C)</p> Signup and view all the answers

What is a characteristic of sex-linked traits?

<p>They often have different expression patterns in males and females. (D)</p> Signup and view all the answers

Given the genotype ccWwtt, what is a possible phenotype?

<p>Recessive traits expressed for c and t, dominant for W (C)</p> Signup and view all the answers

What is a recombinant phenotype?

<p>A phenotype that arises from the combination of alleles from different parents (C)</p> Signup and view all the answers

What is the function of telomerase in linear chromosomes?

<p>To protect chromosome ends from deterioration (B)</p> Signup and view all the answers

How does crossing over relate to genetic recombination?

<p>It leads to the exchange of genetic material between homologous chromosomes (B)</p> Signup and view all the answers

What is the role of DNA ligase during replication?

<p>To join Okazaki fragments together on the lagging strand (D)</p> Signup and view all the answers

Which of these scientists contributed to determining the structure of DNA?

<p>Francis Crick (A), James Watson (B), Rosalind Franklin (D)</p> Signup and view all the answers

In which direction does DNA replication occur?

<p>5' to 3' direction (C)</p> Signup and view all the answers

What is a Barr Body?

<p>A condensed inactive X chromosome found in female mammals (B)</p> Signup and view all the answers

What is the primary purpose of a linkage map in genetics?

<p>To show the arrangement of genes on a chromosome (B)</p> Signup and view all the answers

Which type of mutation is most likely to result in a malfunctioning protein?

<p>Nonsense mutation (B)</p> Signup and view all the answers

What is a characteristic feature of germ line mutations compared to somatic mutations?

<p>Germ line mutations can be inherited (A)</p> Signup and view all the answers

Which of the following is an example of a chromosomal mutation?

<p>Duplication (D)</p> Signup and view all the answers

What process describes the reversion of a mutated gene back to its original state?

<p>Back mutation (B)</p> Signup and view all the answers

In eukaryotes, what additional level of regulation exists compared to prokaryotes?

<p>Enhancer elements (D)</p> Signup and view all the answers

What effect does DNA methylation typically have on gene expression?

<p>Silences gene expression (B)</p> Signup and view all the answers

Which scenario describes the condition when both glucose and lactose are high in the regulation of the Lac operon?

<p>Lac operon is fully repressed (A)</p> Signup and view all the answers

What role do enhancers serve in gene regulation?

<p>Increase the likelihood of transcription (A)</p> Signup and view all the answers

Flashcards

Scientific Method: Parts

Steps of investigating a natural phenomenon; includes observation, hypothesis formation, prediction, experimentation, analysis, and conclusion.

Hypothesis

A testable explanation for an observation or phenomenon.

Independent Variable

The variable that is changed or manipulated by the researcher in an experiment.

Dependent Variable

The variable that is measured or observed in response to changes in the independent variable.

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Replicates

Multiple trials of an experiment.

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What are the four levels of protein structure?

Proteins have four levels of structure: primary, secondary, tertiary, and quaternary. The primary structure is the linear sequence of amino acids. The secondary structure refers to local folding patterns, such as alpha helices and beta sheets. The tertiary structure describes the overall three-dimensional shape of a single polypeptide chain. The quaternary structure arises when multiple polypeptide chains assemble into a functional protein complex.

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What factors affect protein folding?

Factors affecting protein folding include: interactions between amino acid side chains (hydrophobic, electrostatic, hydrogen bonding), chaperone proteins that assist in proper folding, and environmental conditions such as temperature and pH.

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What does denaturation mean?

Denaturation is the process of disrupting the three-dimensional structure of a protein, often leading to loss of function. This can be caused by factors like heat, extreme pH, or chemicals.

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What are the differences between DNA and RNA?

DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are both nucleic acids, but they differ in their structure and function. DNA is double-stranded and contains deoxyribose sugar, while RNA is single-stranded and contains ribose sugar. DNA stores genetic information, while RNA plays a role in protein synthesis.

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Prokaryotic vs. Eukaryotic?

Prokaryotic cells lack a nucleus and other membrane-bound organelles, while eukaryotic cells have a nucleus and other organelles. Prokaryotes are generally smaller and simpler than eukaryotes.

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Plant vs. Animal Cells?

Plant cells have cell walls, chloroplasts for photosynthesis, and large central vacuoles, which are absent in animal cells. Animal cells contain lysosomes for cellular digestion.

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What are the main components of biological membranes?

Biological membranes are composed of phospholipids, forming a bilayer, with embedded proteins. Glycoproteins and glycolipids are also important components, playing roles in cell recognition and signaling.

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What are the types of membrane proteins?

Membrane proteins can be transmembrane (span the membrane), lipid-anchored (covalently attached), peripheral (noncovalently attached), or bound to the extracellular matrix or cytoskeleton.

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What is passive transport?

Passive transport is the movement of molecules across a membrane without requiring energy. It can be simple diffusion (movement down the concentration gradient) or facilitated diffusion (movement down the gradient with the help of a protein).

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What is active transport?

Active transport is the movement of molecules across a membrane against their concentration gradient, requiring energy. It uses specialized proteins and energy sources like ATP.

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Nondisjunction

The failure of homologous chromosomes or sister chromatids to separate properly during cell division (meiosis or mitosis), resulting in an abnormal number of chromosomes in the daughter cells.

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Aneuploidy

A condition where a cell or organism has an abnormal number of chromosomes, either fewer or more than the typical diploid number.

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Polyploidy

A condition where a cell or organism has more than two sets of chromosomes (more than the typical diploid number).

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What are the benefits of exchanging genetic material between homologous chromosomes?

Exchanging genetic material between homologous chromosomes during meiosis (crossing over) increases genetic diversity by creating new combinations of alleles. This genetic diversity is essential for populations to adapt to changing environments and resist diseases.

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True Breeding

Organisms that consistently produce offspring with the same trait when self-fertilized, meaning they have two identical alleles for that trait.

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Hybrid

An offspring resulting from the cross-breeding of two parents with different traits.

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Homozygote

An individual with two identical alleles for a given trait.

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Heterozygote

An individual with two different alleles for a given trait.

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Complete Dominance

One allele completely masks the expression of the other in a heterozygote, resulting in only the dominant phenotype being observed.

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Incomplete Dominance

The phenotype of a heterozygote is an intermediate blend between the two homozygous phenotypes.

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What is a recombinant phenotype?

A phenotype that results from the combination of alleles from two different parents, through processes like crossing over or independent assortment.

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What is a linkage map?

A genetic map that shows the relative positions of genes on a chromosome, based on the frequency of recombination between them.

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Are all genes located in the Nucleus for Eukaryotes?

No. While most genes are located in the nucleus, some genes are found in organelles like mitochondria and chloroplasts.

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What is the relationship of crossing over to genetic recombination?

Crossing over is a process that occurs during meiosis, where homologous chromosomes exchange genetic material. This exchange leads to genetic recombination, which creates new combinations of alleles and increases genetic diversity.

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What are Barr Bodies?

Inactivated X chromosomes in female mammals, forming a condensed, inactive structure within the nucleus.

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What is the method of Replication for DNA?

DNA replication is semi-conservative, meaning each new DNA molecule contains one original strand and one newly synthesized strand.

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Function of Telomerase?

Telomerase is an enzyme that adds repetitive DNA sequences (telomeres) to the ends of chromosomes, preventing degradation and maintaining chromosome integrity.

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Why is Telomerase important in linear chromosomes?

Telomerase ensures that the ends of linear chromosomes are fully replicated during DNA replication, preventing shortening and protecting the genetic information.

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Point Mutation

A change in a single nucleotide within a gene sequence.

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Frameshift Mutation

A genetic mutation caused by the insertion or deletion of nucleotides, leading to a shift in the reading frame, altering the amino acid sequence.

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Spontaneous Mutation

A mutation that occurs naturally without any known external cause.

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Germline Mutation

A mutation that occurs in a germ cell (sperm or egg), which can be passed on to offspring.

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Loss-of-function mutation

A genetic mutation that reduces or eliminates the activity of a gene.

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Transcriptional Regulation

The control of gene expression at the level of RNA transcription.

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Inducible Operon (Lac Operon)

A type of operon that is activated by the presence of a specific inducer molecule. In the Lac operon, lactose acts as the inducer.

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TATA Box

A DNA sequence located in the promoter region of eukaryotic genes that helps position RNA polymerase for transcription initiation.

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Direct Communication in Cells

Three methods of direct communication in animal and plant cells are: 1) Plasmodesmata (plant cells): cytoplasmic connections that allow for direct exchange of molecules. 2) Gap junctions (animal cells): channels that connect the cytoplasm of adjacent cells, facilitating direct exchange. 3) Cell-cell recognition: interactions between molecules on the surfaces of adjacent cells, allowing for communication and coordination.

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Cell Signaling Phases

Cell signaling can be divided into three phases: 1) Reception: The cell detects a signaling molecule (ligand) by binding it to a specific receptor protein. 2) Transduction: The binding of the ligand triggers a series of events inside the cell, converting the signal into a form that can elicit a cellular response. 3) Response: The signal is finally relayed to a specific target molecule or pathway, leading to a change in cell behavior.

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Membrane Receptor Types

Three categories of membrane proteins act as receptors in cell signaling: 1) G protein-coupled receptors (GPCRs): Utilize a G protein to relay the signal. 2) Enzyme-linked receptors: When activated, they can directly act as enzymes or activate other enzymes. 3) Ion channel receptors: Open or close to allow ions to flow across the membrane, changing the cell's electrical potential.

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Intracellular vs Membrane Receptors

Intracellular receptors are located inside the cell and bind to signaling molecules that can cross the cell membrane. Membrane receptors, on the other hand, are embedded within the cell membrane and bind to signaling molecules that are too large or hydrophilic to cross the membrane. Intracellular receptors often influence gene expression, while membrane receptors activate signaling pathways within the cell.

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Cell Signaling Responses

Cellular responses to signaling molecules can be diverse: 1) Changes in gene expression: The signal may stimulate the production or repression of specific proteins. 2) Activation of enzymes: The signal can activate or inhibit specific enzymes within the cell. 3) Altered cell behavior: The signal can lead to changes in cell growth, movement, or other aspects of cellular function.

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Viral Components

All viruses have two components: 1) Genetic material: either DNA or RNA. 2) Protein coat (capsid): surrounds and protects the genetic material. Some viruses also have an envelope: a membrane derived from the host cell that helps the virus enter cells.

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Viruses and Life

Most scientists consider viruses to be non-living entities because: 1) They cannot reproduce independently and rely on host cells. 2) They lack the machinery and metabolic processes of living organisms. 3) They do not exhibit growth or development.

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Lytic Cycle

The lytic cycle is a viral replication process that destroys the host cell. 1) Attachment: The virus attaches to the host cell. 2) Entry: The viral genetic material enters the host cell. 3) Replication: The viral genome takes over the host's cellular machinery to produce new viral components. 4) Assembly: New viral particles are assembled. 5) Release: Newly formed viruses are released from the host cell, often lysing (bursting) it and releasing more viruses to infect other cells.

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Lysogenic Cycle

The lysogenic cycle is a viral replication process that allows the virus to remain dormant within the host cell. 1) Integration: The viral genome integrates into the host cell's DNA. 2) Replication: The viral genome is replicated along with the host cell's DNA. 3) Dormant: The virus may remain dormant for a period. 4) Induction: The virus can reactivate and enter the lytic cycle.

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Retrovirus Cycle

Retroviruses, like HIV, have a unique replication cycle: 1) Entry: The virus enters the host cell. 2) Reverse transcription: The viral RNA is converted into DNA using reverse transcriptase. 3) Integration: The viral DNA integrates into the host cell's genome. 4) Replication: The integrated viral DNA is replicated along with the host cell's DNA. 5) Transcription and translation: The integrated viral DNA is transcribed into viral RNA and translated into viral proteins. 6) Assembly and release: New viral particles are assembled and released from the host cell.

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Study Notes

Exam I Material

  • Studying Life (Sections 1.1-1.2): Understand the scientific method, know the parts of the method, understand and define hypothesis, null hypothesis, independent and dependent variables, and the importance of replicates. Be able to explain how to evaluate data to support or reject a hypothesis. Define inductive and deductive reasoning, give examples of each. Define qualitative and quantitative measurements. Given a scenario, identify the following: observation, hypothesis, null hypothesis, independent variable, dependent variable, experimental group, control group, constants, data, and conclusions. Explain the difference between the everyday understanding of “theory” and a theory in science. List the three domains of life and classify organisms into one of those domains. Define the following in terms of evolution: positive/negative feedback, feedback system and give examples. Define Biology, Natural Selection, independent variable, and dependent variable.

Chapter 2 - Small Molecules and the Chemistry of Life

  • Define matter, atoms, and atomic number, atomic mass, isotopes. Define octet rule, valence number, and electronegativity. Differentiate between elements and compounds. Define the parts of an atom (protons, neutrons, and electrons).
  • Define valence electrons. Understand the periodic table (periods/groups). Define ionic, covalent, non-polar, polar, and hydrogen bonds. Define polar molecules and describe bonds within a molecule of water.
  • Articulate the important properties of water (adhesion, cohesion, why ice floats, and why salt dissolves and oil doesn't). Explain chromatography. Explain the pH scale (acidic, neutral, basic/alkaline).

Chapter 3 – Proteins Carbohydrates and Lipids & Chapter 4- Nucleic Acids (Section 4.1 ONLY)

  • Define and recognize by sight four ways in which carbon atoms have enormous variability in organic compounds. Be able to describe the binding capacities (valences) of C, H, O, N. Be prepared to explain each functional group. Be able to describe how a functional group will react with water, describe the functional properties, and give an example of each functional group in a biological molecule.
  • List and define the three types of isomers. List the four major types of organic molecules and macromolecules, for the images provided. Define monomers and polymers.

Chapter 5– The Cell (Sections 5.1 – 5.4)

  • Differentiate between prokaryotic and eukaryotic cells. Differentiate between plant and animal cells. Describe the arguments for the bacterial origin of mitochondria and chloroplasts. Identify as prokaryotic or eukaryotic, as plant or animal, and describe the structure and function of eukaryotic organelles. (e.g. nucleus, ribosomes, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles). Identify plasmodesmata and types of intercellular junctions in animal cells.

Chapter 6 – Membranes (Sections 6.1, 6.3 – 6.5)

  • Explain the components of biological membranes. Explain the function of phospholipids, glycoproteins, and glycolipids. Explain the difference between transmembrane, lipid anchor, and peripheral membrane proteins and why these are important. Define how things move through membranes without and with proteins, with and without energy.
  • Explain passive transport (simple diffusion, facilitated diffusion), active transport (endocytosis, receptor-mediated endocytosis, exocytosis), pinocytosis, phagocytosis, and the fluid-mosaic model. List factors affecting fluidity.
  • Explain diffusion, osmosis, hypertonic/hypotonic/isotonic solutions and plasmolysis..

Chapter 7 – Cell Signalling (Sections 7.1 & 7.2 only)

  • List three methods of direct communication in animal and plant cells. Describe the three phases of cell signaling.
  • List three categories of membrane proteins that function as receptors in the process of cell signaling. Describe how intracellular receptors differ from membrane receptors in cell signaling.
  • List three responses that may result from cell signaling.

Chapter 16 Viruses (Section 16.3 ONLY)

  • Viruses: List the two components all viruses have and another component some viruses have. Explain why most scientists do not consider viruses to be alive. Explain the lytic cycle of bacteriophages.

Chapter 8 – Energy, Enzymes, Metabolism

  • List the first two laws of thermodynamics and explain how living things obey these laws. Categorize reactions (dehydration synthesis, hydrolysis, photosynthesis, and cellular respiration) as exergonic or endergonic. Explain how exergonic and endergonic reactions are coupled, energy intermediates, and the structure of ATP/ADP molecules.
  • Explain how the cycling of ATP/ADP provides energy for chemical, transport, and mechanical work in a cell. Explain how enzymes work to speed up a reaction and how structure relates to function. Explain how protein enzymes can be denatured. Interpret data from enzyme reaction experiments and explain the effect of different factors on rates of reactions (pH, temperature, substrate concentration, enzyme concentration). Define denaturation. Define inhibition (competitive and non-competitive).

Exam 3 Material (Chapter 9 – Cellular Respiration)

  • Know where glycolysis, the Krebs cycle, and the electron transport chain occur in the cell. Know the inputs and outputs of each process. Calculate the number of ATP molecules produced in glycolysis for each glucose molecule. Explain complete aerobic cellular respiration (theoretical yield, why these aren't reached, why the cell requires oxygen and releases CO2, why aerobic is more effective than anaerobic). Explain what happens when a molecule accepts an electron (reduction/oxidation).

Chapter 10 –Photosynthesis

  • Determine which types of cells are capable of photosynthesis. Explain where in eukaryotic cells does photosynthesis occur. Define the reactants and products of the light-dependent and light-independent reactions (Calvin Cycle). If given a diagram, be able to label the parts of a chloroplast and locate where photosynthesis takes place. Explain what would happen if a plant was exposed to extreme heat or dryness. Explain why plants have a variety of pigments. Explain how the color of light affects the rate of photosynthesis. List and define the differences between C3, C4, and CAM plants.

Chapters 11 - Mitosis / Meiosis Cell Division

  • Explain how binary fission differs from eukaryotic mitosis and identify similarities.
  • Identify stages of the eukaryotic cell cycle and interphase. Describe the stages of mitosis.
  • Explain the purpose of mitosis and the outcome of mitosis.
  • Explain the stages of meiosis.
  • Explain the relationship between homologous chromosomes and chromatids. Explain the purpose and outcome of meiosis.
  • Identify the genetic significance of crossing-over.
  • Define and give examples of nondisjunction, aneuploidy, and polyploidy in humans.

Chapter 12 – Mendel and the gene

  • Define genes, alleles, phenotype, genotype, true breeding, hybrid, homozygote, and heterozygote, and explain how they relate.
  • Work out simple monohybrid and dihybrid crosses to predict possible genotypes, phenotypes, and ratios. Explain the law of segregation and the law of independent assortment. Analyze pedigree charts to interpret information about genotypes and phenotypes. Explain how exceptions to Mendelian genetics (co-dominance, incomplete dominance, multiple alleles, polygenic inheritance, and environmental effects) affect predictions about inheritance.

Chapter 13 – Molecular Basis of Inheritance

  • Describe the fundamental structure of a nucleotide. Describe how DNA replicates, and the role of the different enzymes in replication. Define and understand processes relating to DNA replication in prokaryotes and eukaryotes. Define telomeres and telomerase. Understand DNA repair (nucleotide excision repair). Know and understand Chargaff's rule. Understand the relationship between crossing-over, genetic recombination. Define purines and pyrimidines.

Chapter 14 - Gene Expression

  • Define and describe the Central Dogma. Describe the three stages of transcription (initiation, elongation, and termination). Explain how RNA processing occurs in eukaryotes. Describe the three stages of translation (initiation, elongation, and termination). Identify the components required for translation and their roles.

Chapter 15 - Mutations

  • Explain the definition and types of mutations (point mutations, silent mutations, missense mutations, nonsense mutations, substitutions, additions, deletions, frameshifts). Explain which mutations cause the most damage. Identify the difference between spontaneous and induced mutations, as well as germ-line and somatic mutations.

Last Chapter on Final Exam (Chapter 16 - Gene Regulation)

  • Explain the benefits of gene regulation in prokaryotes and eukaryotes, which includes the extra level of regulation in eukaryotes. Explain the difference between a repressible operon (e.g., tryptophan operon) and an inducible operon (e.g., lactose operon). Explain the situations (high glucose/high lactose, high glucose/low lactose, low glucose/low lactose, and low glucose/high lactose). Explain how the repressors/activators operate. Describe the parts of the core promoter region in eukaryotic genes (e.g., TATA box, initiation site).
  • Explain what activators and enhancers are. Describe the function of DNA methylation and acetylation. Describe the effect of chromatin remodeling on gene expression.

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Prepare for your Biology Exam I by studying key concepts from Sections 1.1-1.2, focusing on the scientific method, hypothesis formulation, and the classification of organisms. You'll explore independent and dependent variables, data evaluation, and the nuances of inductive vs. deductive reasoning. This quiz aims to reinforce your understanding of fundamental biology principles and their applications in scientific research.

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