Podcast
Questions and Answers
Who first observed cells and named them in 1665?
Who first observed cells and named them in 1665?
- Theodor Schwann
- Rudolf Virchow
- Robert Hooke (correct)
- Matthias Schleiden
What principle did Rudolf Virchow introduce to the Cell Theory in 1855?
What principle did Rudolf Virchow introduce to the Cell Theory in 1855?
- Every cell arises from a pre-existing one. (correct)
- The cell is the basic unit of life.
- Cells are the smallest units of matter.
- All living organisms are made up of cells.
What percentage of a cell's composition is water?
What percentage of a cell's composition is water?
- 70% (correct)
- 80%
- 50%
- 60%
Which of the following is NOT a key element that cells are composed of?
Which of the following is NOT a key element that cells are composed of?
What is the primary function of the plasma membrane in a cell?
What is the primary function of the plasma membrane in a cell?
Which of the following statements is part of the Cell Theory?
Which of the following statements is part of the Cell Theory?
What component of the cell provides a medium for metabolic reactions?
What component of the cell provides a medium for metabolic reactions?
Which of the following is NOT a characteristic shared by all cells?
Which of the following is NOT a characteristic shared by all cells?
What is the primary role of ATP in the cell?
What is the primary role of ATP in the cell?
Which of the following defines the function of NAD and NADP?
Which of the following defines the function of NAD and NADP?
What is Acetyl-CoA mainly involved in?
What is Acetyl-CoA mainly involved in?
What triggers the release of energy from ATP?
What triggers the release of energy from ATP?
What is vesicular trafficking primarily responsible for?
What is vesicular trafficking primarily responsible for?
Which molecule is involved in the citric acid cycle to extract energy?
Which molecule is involved in the citric acid cycle to extract energy?
Which component is essential for membrane trafficking?
Which component is essential for membrane trafficking?
How do NAD and NADP molecules alternate during metabolic reactions?
How do NAD and NADP molecules alternate during metabolic reactions?
What do sugars combine to form, which provides energy and structural support?
What do sugars combine to form, which provides energy and structural support?
Which process involves the removal of water to form covalent bonds between monomers?
Which process involves the removal of water to form covalent bonds between monomers?
What is the typical size range for most eukaryotic cells?
What is the typical size range for most eukaryotic cells?
What factor is crucial for the efficiency of nutrient exchange in cells?
What factor is crucial for the efficiency of nutrient exchange in cells?
What are activated carrier molecules responsible for in a cell?
What are activated carrier molecules responsible for in a cell?
Which of the following is NOT an example of an activated carrier molecule?
Which of the following is NOT an example of an activated carrier molecule?
How does cell size affect metabolism when a cell grows larger?
How does cell size affect metabolism when a cell grows larger?
What role do cell-cell junctions play in multicellular structures?
What role do cell-cell junctions play in multicellular structures?
What is primarily responsible for the selective barrier function of the plasma membrane?
What is primarily responsible for the selective barrier function of the plasma membrane?
Which component of the plasma membrane is crucial for maintaining its fluidity?
Which component of the plasma membrane is crucial for maintaining its fluidity?
What does membrane asymmetry allow for in a cell?
What does membrane asymmetry allow for in a cell?
How do most ions and larger molecules cross the plasma membrane?
How do most ions and larger molecules cross the plasma membrane?
What establishes the directional organization of the plasma membrane during vesicle fusion?
What establishes the directional organization of the plasma membrane during vesicle fusion?
Which lipids are more abundant in the extracellular leaflet of the plasma membrane?
Which lipids are more abundant in the extracellular leaflet of the plasma membrane?
What is a critical role of cholesterol in the plasma membrane?
What is a critical role of cholesterol in the plasma membrane?
What is one of the essential functions of the plasma membrane?
What is one of the essential functions of the plasma membrane?
What role do vesicles play in hormone secretion?
What role do vesicles play in hormone secretion?
Which type of proteins assist vesicles in moving along the cytoskeleton?
Which type of proteins assist vesicles in moving along the cytoskeleton?
How does compartmentalization of organelles benefit cellular processes?
How does compartmentalization of organelles benefit cellular processes?
What is the significance of contact points in cellular trafficking?
What is the significance of contact points in cellular trafficking?
What does the dynamic nature of cellular processes reveal about traditional textbook representations?
What does the dynamic nature of cellular processes reveal about traditional textbook representations?
In neurons, what process is facilitated by vesicles containing neurotransmitters?
In neurons, what process is facilitated by vesicles containing neurotransmitters?
Which organelle is specifically mentioned as having an acidic environment for breaking down macromolecules?
Which organelle is specifically mentioned as having an acidic environment for breaking down macromolecules?
What is the overall purpose of vesicular trafficking in multicellular organisms?
What is the overall purpose of vesicular trafficking in multicellular organisms?
What is the primary function of the nucleus in a cell?
What is the primary function of the nucleus in a cell?
What structure encloses the nucleus?
What structure encloses the nucleus?
Why are nuclear pores important?
Why are nuclear pores important?
What role does the nucleolus play within the nucleus?
What role does the nucleolus play within the nucleus?
What happens to chromatin during DNA transcription?
What happens to chromatin during DNA transcription?
Which of the following best describes the dynamic nature of the nucleus?
Which of the following best describes the dynamic nature of the nucleus?
What typically influences the number of nucleoli within a cell?
What typically influences the number of nucleoli within a cell?
What is the diameter of the nucleus, on average?
What is the diameter of the nucleus, on average?
Flashcards
Cell
Cell
The smallest and most fundamental unit of life, responsible for all biological processes.
Cell Theory
Cell Theory
The concept that all living organisms are composed of cells, cells are the basic unit of life, and new cells arise from pre-existing cells.
Robert Hooke
Robert Hooke
A scientist who observed plant tissue under a microscope and named the small, chamber-like structures "cells", laying the groundwork for the Cell Theory.
Matthias Schleiden and Theodor Schwann
Matthias Schleiden and Theodor Schwann
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"Omnis cellula e cellula"
"Omnis cellula e cellula"
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Plasma membrane
Plasma membrane
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Cytoplasm
Cytoplasm
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Nucleus
Nucleus
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Macromolecules
Macromolecules
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Monosaccharides
Monosaccharides
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Fatty acids
Fatty acids
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Amino acids
Amino acids
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Nucleotides
Nucleotides
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Condensation reaction
Condensation reaction
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Hydrolysis
Hydrolysis
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Activated carrier molecules
Activated carrier molecules
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What is ATP?
What is ATP?
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What are NAD/NADP?
What are NAD/NADP?
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What is FAD?
What is FAD?
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What is Acetyl-CoA?
What is Acetyl-CoA?
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What is membrane trafficking?
What is membrane trafficking?
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What are vesicles?
What are vesicles?
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What is vesicular trafficking?
What is vesicular trafficking?
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What are organelle contact points?
What are organelle contact points?
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Vesicular Trafficking
Vesicular Trafficking
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Organelles
Organelles
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Organelle Compartmentalization
Organelle Compartmentalization
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Cytoskeleton
Cytoskeleton
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Motor Proteins (e.g., Kinesins and Dyneins)
Motor Proteins (e.g., Kinesins and Dyneins)
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Contact Points
Contact Points
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Real-Time Cellular Imaging
Real-Time Cellular Imaging
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Limitations of Fixed Cells
Limitations of Fixed Cells
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What is the nucleus?
What is the nucleus?
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What is the nuclear envelope?
What is the nuclear envelope?
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What are nuclear pores?
What are nuclear pores?
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What is the nucleolus?
What is the nucleolus?
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What molecules move through nuclear pores?
What molecules move through nuclear pores?
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Is the nucleus static or dynamic?
Is the nucleus static or dynamic?
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How does chromatin change during transcription?
How does chromatin change during transcription?
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Why is the nucleus important?
Why is the nucleus important?
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Phospholipid Bilayer
Phospholipid Bilayer
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Membrane Asymmetry
Membrane Asymmetry
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Membrane Polarity
Membrane Polarity
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Passive Transport
Passive Transport
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Active Transport
Active Transport
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Endocytosis
Endocytosis
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Exocytosis
Exocytosis
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Study Notes
The Cell: The Fundamental Unit of Life
- Cells are the basic units of all living organisms
- Responsible for all life processes including growth, reproduction, and function
- First recognized by Robert Hooke in 1665
- Cell Theory, proposed by Matthias Schleiden and Theodor Schwann (1838-39), states all living organisms are composed of cells, the cell is the basic unit of life, and new cells arise only from pre-existing cells
- Cell Theory expanded in 1855 by Rudolf Virchow with the principle “omnis cellula e cellula” – every cell arises from a pre-existing cell
Universal Features of Cells
- All cells share common characteristics
- Plasma membrane: boundary between interior and exterior
- Cytoplasm: medium for metabolic reactions
- Nucleus (eukaryotic cells): Houses genetic material
- Cells are primarily composed of water (~70%) and six key elements (H, C, O, N, S, P)
Macromolecule Composition
- Sugars: form polysaccharides, provide energy and structure
- Fatty acids: form lipids, for cell membranes and energy storage
- Amino acids: form proteins, with diverse functions (catalysing reactions, transporting molecules)
- Nucleotides: form nucleic acids (DNA, RNA), store and transmit genetic information
Understanding Cell Size and Limitations
- Eukaryotic cells: 10-100 microns
- Prokaryotic cells (bacteria): 1-50 microns
- Small size is crucial for efficient exchange of nutrients, gases, and waste with the environment
- Surface-to-volume ratio impacts a cell's ability to sustain itself as it grows.
Energy and Cellular Processes
- Cells need energy for essential functions (movement, synthesis, maintaining stability)
- Stored in activated carrier molecules (ATP, NAD, NADP, FAD, Acetyl-CoA)
- Easily exchangeable and transportable, powering a wide range of cellular processes.
Activated Carrier Molecules
- ATP: the primary energy currency, releases energy when bonds are broken. Fuels active transport, cell motility and synthesis of macromolecules like DNA and proteins.
- NAD/NADP: Electron carriers, alternate between oxidized (NAD+/NADP+) and reduced (NADH/NADPH) states, critical for redox reactions, transferring electrons for energy production.
- FAD: Similar electron carrier function to NAD and NADP. Key in the citric acid cycle, extracting energy from organic molecules.
- Acetyl-CoA: Central metabolic intermediate, links carbohydrate, fat, and protein metabolism.
Membrane Trafficking
- Movement of materials within cells (between organelles and plasma membrane)
- Vesicular Trafficking: Small membrane-bound carriers transport molecules between compartments
- Organelle Trafficking and Compartmentalization: Specific organelles (like lysosomes) isolate reactions to maintain proper cellular functions
The Plasma Membrane: Structure and Function
- Defines the cell boundary, regulates molecule transport, and facilitates communication
- Primarily composed of a phospholipid bilayer with embedded proteins, cholesterol, and other lipids.
- Amphipathic nature (hydrophilic heads, hydrophobic tails) creates a selective barrier.
- Regulates movement of molecules in and out of the cell
- Plays roles in cell signaling and communication, intercellular adhesion, and maintaining intracellular/extracellular environments.
The Plasma Membrane: Asymmetry and Polarity
- Asymmetry: uneven distribution of phospholipids across the inner and outer layers. Essential to biological processes
- Polarity: Membrane layers display distinct directional organization, vital for processes like secretion and endocytosis.
Membrane Proteins and Lipid Modifications
- Integral membrane proteins: span the bilayer; function as transporters, receptors, enzymes
- Peripheral membrane proteins: located on membrane surface; often interact with integral proteins
- Lipid modifications (like GPI anchoring) play roles in anchoring and localization of specific membrane proteins in precise locations.
Cytoskeleton: Structure and Function
- Dynamic network of protein filaments that provides structural support and facilitates intracellular transport and cell movement.
- Intermediate Filaments: Strong, rope-like components providing structural strength
- Microtubules: Hollow tubes involved in cell shape, movement, intracellular transport; grow and shrink regularly; originate from the centrosome
- Actin filaments: Thinnest; involved in processes like cell shape, movement, and intracellular transport
The Endoplasmic Reticulum (ER)
- Extensive network of membranes and sacs called cisternae; continuous with the nuclear envelope.
- Rough ER: Studded with ribosomes; site of protein synthesis and modification (e.g., glycosylation)
- Smooth ER: Lacks ribosomes; involved in lipid synthesis, detoxification of harmful substances, and calcium storage.
Mitochondria
- "Powerhouses of the cell"; responsible for ATP generation via oxidative phosphorylation.
- Double membrane system (outer and inner); inner membrane highly folded into cristae to maximize surface area for protein complexes.
- Site of the Krebs (citric acid) cycle, generating electron carriers (NADH, FADH2) that drive ATP production in the electron transport chain.
Nucleus: The Control Center
- The cell's control center, containing genetic material (DNA)
- Separates genetic material from cytoplasm
- Encloses DNA; site of transcription (DNA to RNA)
- Nuclear envelope: double membrane with nuclear pores allowing specific molecules to pass through
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