Biology Chapter on Protein Structure
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Questions and Answers

What is the basic unit of proteins?

  • Amino acid (correct)
  • Nucleotide
  • Fatty acid
  • Carbohydrate

Which of the following is NOT a level of protein structure?

  • Primary
  • Secondary
  • Hexagonal (correct)
  • Tertiary

What type of bond links amino acids together in a protein?

  • Peptide bond (correct)
  • Ionic bond
  • Disulfide bridge
  • Hydrogen bond

Which group of amino acids is typically found in the R group?

<p>Hydrophobic group (D)</p> Signup and view all the answers

What describes the secondary structure of proteins?

<p>Folding of alpha helices (C)</p> Signup and view all the answers

What is the role of the initiation codon?

<p>It starts the process of protein synthesis. (B)</p> Signup and view all the answers

Why is the genetic code described as degenerate?

<p>Multiple codons can specify the same amino acid. (B)</p> Signup and view all the answers

What feature of the reading frame affects how codons are interpreted?

<p>It dictates that codons are read in groups of three nucleotides. (D)</p> Signup and view all the answers

What is the role of aminoacyl-tRNA synthetases?

<p>They charge tRNA with the correct amino acid. (B)</p> Signup and view all the answers

Which of the following describes nonsense codons?

<p>They specify no amino acid. (D)</p> Signup and view all the answers

What is required for the initiation of translation in eukaryotes?

<p>5' cap and 3' cap (D)</p> Signup and view all the answers

Which site in the ribosome is responsible for tRNA losing its amino acid?

<p>Exit site (E) (B)</p> Signup and view all the answers

What are the codons that signal termination in translation?

<p>UAA, UAG, UGA (B)</p> Signup and view all the answers

What begins the amino acid sequence during translation?

<p>Met (Methionine) (C)</p> Signup and view all the answers

Which of the following is NOT a post-translational modification of proteins?

<p>Peptide bond formation (D)</p> Signup and view all the answers

What is the primary role of tRNA during translation?

<p>To bring amino acids to the ribosome (A)</p> Signup and view all the answers

How many subunits compose eukaryotic ribosomes?

<p>Two subunits of 60S and 40S (C)</p> Signup and view all the answers

Which component is NOT required for the process of translation?

<p>Nucleus (B)</p> Signup and view all the answers

What is the function of the Shine-Dalgarno sequence in prokaryotic initiation?

<p>It promotes ribosome binding to mRNA (D)</p> Signup and view all the answers

What distinguishes prokaryotic ribosomes from eukaryotic ribosomes?

<p>Prokaryotic ribosomes consist of fewer subunits (C)</p> Signup and view all the answers

Flashcards

Amino acid structure

An organic molecule with an amino group, carboxyl group, and a variable R-group bonded to a central carbon atom.

Peptide bond

The chemical bond connecting amino acids in a polypeptide chain.

Protein Translation

The process of converting mRNA into a sequence of amino acids forming a protein.

Primary Protein Structure

The linear sequence of amino acids in a protein.

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Secondary Protein Structure

Local folding of the polypeptide chain into structures like alpha-helices.

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Codon

A sequence of three nucleotides in mRNA that codes for a specific amino acid.

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Start Codon

The mRNA codon (AUG) that signals the beginning of protein synthesis.

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Reading Frame

The way a sequence of nucleotides is read in groups of three to determine the order of amino acids in a protein.

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tRNA

Transfer RNA; molecule that carries amino acids to the ribosome during protein synthesis.

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Aminoacyl-tRNA synthetases

Enzymes that attach the correct amino acid to its corresponding tRNA.

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tRNA Wobble

The ability of some tRNA's to recognize multiple codons due to flexibility in the third base pairing position. This allows for fewer tRNA's to decode the genetic code.

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Eukaryotic Ribosomes

Larger ribosomes found in eukaryotes, composed of a 60S and a 40S subunit. They are responsible for protein synthesis in eukaryotic cells.

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Prokaryotic Ribosomes

Smaller ribosomes found in prokaryotes, composed of a 50S and a 30S subunit. They are essential for protein synthesis in bacteria.

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Shine-Dalgarno Sequence

A sequence of nucleotides near the start codon in bacterial mRNA that helps the ribosome identify the correct start site for translation.

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Initiation Factors

Proteins that help initiate the translation process in prokaryotes. They bind to the mRNA, ribosome, and initiator tRNA to set up the complex for translation.

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Eukaryotic Translation Initiation

The process of starting protein synthesis in eukaryotes, requiring a 5' cap and 3' poly(A) tail on the mRNA.

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Ribosome Sites

The ribosome has three sites: A (aminoacyl) for tRNA carrying amino acids, P (peptidyl) for growing polypeptide chain, and E (exit) for tRNA release.

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Elongation Factors

Proteins that assist in the elongation phase of translation in eukaryotes, such as $T_a$, $T_s$, and $G_a$.

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Translation Termination

The process of ending protein synthesis, triggered by stop codons (UAA, UAG, UGA) which signal release factors to bind to the A site.

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Post-Translational Modifications

Changes made to a protein after translation, such as adding carbohydrates or lipids.

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