Biology Chapter on Osmosis and Organisms
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Questions and Answers

What term describes organisms that thrive in high salt concentrations?

  • Osmophile
  • Isotonic
  • Halophile (correct)
  • Hypotonic
  • What occurs when a cell is placed in a hypertonic solution?

  • Cell remains unchanged
  • Cell swells due to excess water
  • Cell gains solute from the environment
  • Cell loses water and shrinks (correct)
  • Which of the following describes a solution that has the same osmotic pressure as another solution?

  • Plasmolysis
  • Hypertonic solution
  • Hypotonic solution
  • Isotonic solution (correct)
  • What is the process called when a cell loses water and becomes flaccid?

    <p>Plasmolysis (B)</p> Signup and view all the answers

    Which term refers to organisms that thrive in environments with high concentrations of solutes other than salt?

    <p>Osmophile (D)</p> Signup and view all the answers

    What occurs to a cell when it is placed in a hypotonic solution?

    <p>The cell gains water and may burst. (D)</p> Signup and view all the answers

    What is the effect on a cell placed in a hypertonic solution?

    <p>The cell loses water and shrinks. (B)</p> Signup and view all the answers

    What characterizes an isotonic solution in relation to a cell?

    <p>Water moves in and out of the cell at equal rates. (D)</p> Signup and view all the answers

    How does a plant cell respond to being placed in a hypertonic solution?

    <p>It undergoes plasmolysis and may wilt. (D)</p> Signup and view all the answers

    In what situation does a cell remain unchanged when placed in a solution?

    <p>When in an isotonic solution. (A)</p> Signup and view all the answers

    What is an antiseptic?

    <p>A substance that kills or inhibits the growth of microorganisms on living tissue (A)</p> Signup and view all the answers

    What is the purpose of the filter paper disk method in evaluating antiseptics?

    <p>To determine the effectiveness of the antiseptic against bacteria (B)</p> Signup and view all the answers

    What is the filter paper disk method used for evaluating antiseptics?

    <p>Soaking filter paper disks in antiseptic and placing them on agar plates to observe zone of inhibition. (A)</p> Signup and view all the answers

    What is the primary function of antiseptics?

    <p>To reduce or eliminate microbial load on living tissues (B)</p> Signup and view all the answers

    Why must antiseptics be used at lower concentrations than disinfectants?

    <p>Higher concentrations can cause tissue damage (D)</p> Signup and view all the answers

    How do disinfectants manage higher concentrations than antiseptics?

    <p>They are applied only to non-living surfaces (B)</p> Signup and view all the answers

    Which of the following factors does NOT affect the efficacy of antiseptics and disinfectants?

    <p>Color of the solution (D)</p> Signup and view all the answers

    What type of chemicals are commonly used as antiseptics?

    <p>Ethyl alcohol and halogens (B)</p> Signup and view all the answers

    Which of the following is a characteristic of disinfectants?

    <p>They are aimed at eliminating contamination on non-living surfaces (C)</p> Signup and view all the answers

    What directly impacts the stability and effectiveness of antiseptics and disinfectants?

    <p>Their chemical properties and structure (D)</p> Signup and view all the answers

    Which of the following is NOT a mechanism through which antiseptics and disinfectants act?

    <p>Producing a toxic gas (A)</p> Signup and view all the answers

    What are antibiotics primarily used for?

    <p>To treat bacterial infections (B)</p> Signup and view all the answers

    Which method is commonly used for antibiotic sensitivity testing?

    <p>Kirby-Bauer disk diffusion method (C)</p> Signup and view all the answers

    What is a crucial factor affecting the efficacy of antibiotics?

    <p>Concentration of the antibiotic (B)</p> Signup and view all the answers

    How do antibiotics exert their action on bacterial cells?

    <p>By disrupting cell wall synthesis (B)</p> Signup and view all the answers

    Which of the following statements about antibiotic sensitivity testing is false?

    <p>Sensitivity testing is only necessary for viral infections. (B)</p> Signup and view all the answers

    What is the primary difference between antibiotics and antimicrobics?

    <p>Antibiotics are derived from natural sources, whereas antimicrobics can be synthetic or semi-synthetic. (A)</p> Signup and view all the answers

    Which of the following describes a broad spectrum antibiotic?

    <p>An antibiotic that can kill both gram-positive and gram-negative bacteria. (A)</p> Signup and view all the answers

    What characterizes a narrow spectrum antibiotic?

    <p>It specifically targets only a few types of bacteria. (A)</p> Signup and view all the answers

    Which of the following statements is true regarding the effective concentration of antibiotics?

    <p>Effective concentration can vary depending on the type of pathogen treated. (C)</p> Signup and view all the answers

    What is a characteristic property of broad spectrum antibiotics?

    <p>They can disrupt normal flora in the body. (D)</p> Signup and view all the answers

    What is the primary role of enzymes in metabolism?

    <p>To catalyze biochemical reactions (D)</p> Signup and view all the answers

    Which of the following best describes exoenzymes?

    <p>Enzymes secreted outside the cell to break down substrates (B)</p> Signup and view all the answers

    What distinguishes oxidation from fermentation?

    <p>Fermentation produces energy without oxygen, while oxidation requires it (B)</p> Signup and view all the answers

    What is the defining characteristic of endoenzymes?

    <p>They catalyze reactions inside the cell (D)</p> Signup and view all the answers

    Which statement accurately defines fermentation?

    <p>A way to generate energy in the absence of oxygen (A)</p> Signup and view all the answers

    Which of the following best defines metabolism?

    <p>The sum of all chemical reactions that occur within a living organism. (A)</p> Signup and view all the answers

    What role does phenol red play in sugar fermentation tests?

    <p>It acts as a color indicator for pH changes. (C)</p> Signup and view all the answers

    Which media are used in the mixed acid fermentation test and butanediol fermentation test?

    <p>MR-VP broth and phenol red broth. (B)</p> Signup and view all the answers

    What does a positive result indicate in the catalase test?

    <p>The release of gas bubbles. (D)</p> Signup and view all the answers

    Which of the following bacterial genera is known to be oxidase positive?

    <p>Pseudomonas. (B)</p> Signup and view all the answers

    What is the significance of identifying a bacterium as catalase positive?

    <p>It confirms the bacterium can detoxify harmful hydrogen peroxide. (A)</p> Signup and view all the answers

    Flashcards

    Atomic Concept

    The smallest unit of a concept that can be understood and memorized

    Testing Effect

    Active recall enhances memory better than rereading.

    Progressive Learning

    Building new knowledge upon previous concepts.

    Memory Tip

    Technique to aid in information recall.

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    Flashcard

    Learning tool with a question on one side; answer on the other.

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    Concise Explanation

    Brief, clear explanation of a concept.

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    Retrieval Practice

    Actively trying to remember information

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    Core Concept

    The most essential ideas in a topic.

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    Atomic Explanations

    Detailed, individual core concepts

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    Mnemonics

    Memory aiding techniques.

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    Halophile

    Organism that thrives in high salt concentrations.

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    Osmophile

    Organism that thrives in high sugar concentrations.

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    Hypertonic solution

    Solution with higher solute concentration than a cell.

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    Hypotonic solution

    Solution with lower solute concentration than a cell.

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    Plasmolysis

    Cell membrane pulling away from the cell wall due to water loss.

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    Hypertonic solution effect on a cell

    Water moves out of the cell, causing it to shrink.

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    Hypotonic solution effect on a cell

    Water moves into the cell, causing it to swell.

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    Isotonic solution effect on a cell

    Water moves in and out of the cell at equal rates, no change in size.

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    Cell in hypertonic solution

    A cell loses water and shrinks.

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    Cell in hypotonic solution

    A cell gains water and swells.

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    Antiseptic function

    Antiseptics prevent infection on living tissues by inhibiting or killing microorganisms without necessarily sterilizing, reducing microbial load.

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    Disinfectant function

    Disinfectants prevent or control infections on non-living surfaces by killing or inhibiting microorganisms, reducing contamination.

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    Effective concentration (antiseptics/disinfectants)

    The specific amount needed for an antiseptic or disinfectant to be effective, varying by the agent, microbe target, and organic matter, expressed as percentages or dilutions.

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    Antiseptic concentration limit

    Antiseptics have lower effective concentrations than disinfectants due to potential tissue damage at higher concentrations.

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    Disinfectant concentration

    Disinfectants can tolerate higher concentrations due to their application on inanimate objects, can be effective over a range of concentrations.

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    Antiseptic types

    Common antiseptic types include alcohols (ethyl, isopropyl), halogens (chlorine, iodine), and phenols.

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    Disinfectant types

    Common disinfectant types include bleach (sodium hypochlorite), quaternary ammonium compounds, and aldehydes (glutaraldehyde).

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    Factors affecting antiseptic/disinfectant efficacy

    Contact time, temperature, and the material being treated affect the effectiveness of antiseptics and disinfectants, concentration must be adjusted accordingly.

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    Antibiotics

    Drugs that kill or inhibit the growth of bacteria.

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    Antibiotic Sensitivity Testing

    A lab technique to determine which antibiotics are effective against a specific bacteria.

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    Method for antibiotic sensitivity testing

    The Kirby-Bauer disk diffusion method measures bacterial sensitivity to different antibiotics.

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    Kirby-Bauer method

    A lab technique used to find best antibiotic to kill or stop growth of bacteria.

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    Bacterial growth inhibition

    The prevention of bacteria multiplication caused by resistance to antibiotics.

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    Antibiotic vs. Antimicrobic

    Antibiotics target bacteria specifically; antimicrobics target a broader range of microorganisms, including bacteria, fungi, and viruses.

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    Narrow Spectrum Antibiotic

    An antibiotic that is effective against a limited range of bacteria.

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    Broad Spectrum Antibiotic

    An antibiotic effective against a wide variety of bacteria.

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    Antibiotic targets

    Specific processes in bacterial cells, like their cell wall construction or protein synthesis.

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    Antibiotic action

    Interfering with bacterial growth, often by inhibiting the synthesis of vital compounds for bacteria.

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    Metabolism

    The sum of all chemical reactions in a living organism, including building (anabolism) and breaking down (catabolism) molecules.

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    Enzymes

    Biological catalysts that speed up chemical reactions in living organisms.

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    Endoenzyme

    Enzymes that function inside the cell that produces them.

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    Exoenzyme

    Enzymes that function outside the cell that produces them.

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    Oxidation

    A chemical reaction where a substance loses electrons.

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    Sugar Fermentation Tests

    Tests used to identify bacteria based on their ability to ferment sugars, producing acid and gas.

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    Mixed Acid Fermentation Test

    A test that identifies bacteria based on the production of various acids (like lactic, acetic, formic acid) during sugar fermentation.

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    Catalase Test

    A test determining if a bacteria produces the enzyme catalase, which breaks down hydrogen peroxide.

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    Oxidase Test

    A test to detect bacteria that contain the enzyme cytochrome c oxidase, involved in electron transport

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    Oxidase-positive genera

    Groups of bacteria that possess the enzyme cytochrome c oxidase, which is involved in electron transport.

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