Podcast
Questions and Answers
What is the primary function of flagella in prokaryotic cells?
What is the primary function of flagella in prokaryotic cells?
- Cell division
- Protein synthesis
- Nutrient absorption
- Locomotion (correct)
What distinguishes Gram-positive bacteria from Gram-negative bacteria?
What distinguishes Gram-positive bacteria from Gram-negative bacteria?
- Gram-negative bacteria have no cell wall.
- Gram-negative bacteria always cause disease.
- Gram-positive bacteria have a thinner cell wall.
- Gram-positive bacteria stain purple due to a thicker peptidoglycan layer. (correct)
Which characteristic is exclusive to eukaryotic cells?
Which characteristic is exclusive to eukaryotic cells?
- Undergoing binary fission
- Containing peptidoglycan
- Having a membrane-bound nucleus (correct)
- Possessing a cytoskeleton
What component is not part of the general structure of a cell?
What component is not part of the general structure of a cell?
What is the content of the cytoplasm composed of?
What is the content of the cytoplasm composed of?
Which of the following cell types does not belong to the category of eukaryotic cells?
Which of the following cell types does not belong to the category of eukaryotic cells?
What is the function of the cytoskeleton in eukaryotic cells?
What is the function of the cytoskeleton in eukaryotic cells?
Gram-negative bacteria are characterized by which of the following?
Gram-negative bacteria are characterized by which of the following?
What is the primary function of cells according to the cell theory?
What is the primary function of cells according to the cell theory?
What is the estimated number of cells in the human body?
What is the estimated number of cells in the human body?
Which of the following statements regarding various cell types in the human body is accurate?
Which of the following statements regarding various cell types in the human body is accurate?
Which of the following is NOT a part of the cell theory?
Which of the following is NOT a part of the cell theory?
What type of cells are neurons classified as?
What type of cells are neurons classified as?
Which cell type is primarily responsible for carrying oxygen in the bloodstream?
Which cell type is primarily responsible for carrying oxygen in the bloodstream?
Which of the following cell types is classified as a connective tissue cell?
Which of the following cell types is classified as a connective tissue cell?
What is the role of the plasma membrane in a cell?
What is the role of the plasma membrane in a cell?
What is the structural composition of centrioles within the centrosome?
What is the structural composition of centrioles within the centrosome?
Which organelle is primarily responsible for protein synthesis?
Which organelle is primarily responsible for protein synthesis?
What is the main function of cilia in the respiratory system?
What is the main function of cilia in the respiratory system?
What distinguishes rough endoplasmic reticulum from smooth endoplasmic reticulum?
What distinguishes rough endoplasmic reticulum from smooth endoplasmic reticulum?
Which of the following is NOT a function of the smooth endoplasmic reticulum?
Which of the following is NOT a function of the smooth endoplasmic reticulum?
What role do the basilar bodies play in relation to cilia and flagella?
What role do the basilar bodies play in relation to cilia and flagella?
What is the primary function of the centrosome during cell division?
What is the primary function of the centrosome during cell division?
How are ribosomes produced within the cell?
How are ribosomes produced within the cell?
How does cholesterol affect membrane fluidity at warm temperatures?
How does cholesterol affect membrane fluidity at warm temperatures?
What is the role of glycolipids in the plasma membrane?
What is the role of glycolipids in the plasma membrane?
What distinguishes integral membrane proteins from peripheral membrane proteins?
What distinguishes integral membrane proteins from peripheral membrane proteins?
What is one function of transmembrane proteins?
What is one function of transmembrane proteins?
Where are peripheral membrane proteins found in relation to the membrane?
Where are peripheral membrane proteins found in relation to the membrane?
What is the glycoprotein's significance in the plasma membrane?
What is the glycoprotein's significance in the plasma membrane?
What is the glycocalyx composed of?
What is the glycocalyx composed of?
How does cholesterol maintain membrane fluidity at cold temperatures?
How does cholesterol maintain membrane fluidity at cold temperatures?
What distinguishes unipotent stem cells from non-stem cells?
What distinguishes unipotent stem cells from non-stem cells?
Which stem cell type can give rise to nearly all cell types derived from any of the three germ layers?
Which stem cell type can give rise to nearly all cell types derived from any of the three germ layers?
What is the primary characteristic of oligopotent stem cells?
What is the primary characteristic of oligopotent stem cells?
Which of the following statements about prokaryotic cells is correct?
Which of the following statements about prokaryotic cells is correct?
What is the primary function of cell walls in prokaryotic cells?
What is the primary function of cell walls in prokaryotic cells?
Which of these is NOT a characteristic of all cell types?
Which of these is NOT a characteristic of all cell types?
What is the relationship between totipotent and pluripotent stem cells?
What is the relationship between totipotent and pluripotent stem cells?
Which type of stem cell can differentiate into a number of cell types, but only those related to each other?
Which type of stem cell can differentiate into a number of cell types, but only those related to each other?
Which characteristic is unique to ribosomes present in prokaryotic cells?
Which characteristic is unique to ribosomes present in prokaryotic cells?
What is indicated by asymmetric stem cell division?
What is indicated by asymmetric stem cell division?
What is NOT a function of lysosomes?
What is NOT a function of lysosomes?
Which structure is primarily involved in detoxifying harmful substances in the liver?
Which structure is primarily involved in detoxifying harmful substances in the liver?
What characterizes the Golgi complex?
What characterizes the Golgi complex?
What is the main function of proteasomes?
What is the main function of proteasomes?
Which of the following conditions is linked to a missing lysosomal enzyme?
Which of the following conditions is linked to a missing lysosomal enzyme?
What do peroxisomes primarily utilize molecular oxygen for?
What do peroxisomes primarily utilize molecular oxygen for?
Which statement about lysosomes is accurate?
Which statement about lysosomes is accurate?
What is the structure of a proteasome?
What is the structure of a proteasome?
Which type of stem cell is isolated from the inner cell mass of blastocysts?
Which type of stem cell is isolated from the inner cell mass of blastocysts?
What is the primary role of totipotent stem cells in early embryonic development?
What is the primary role of totipotent stem cells in early embryonic development?
What defines the term 'potency' in the context of stem cells?
What defines the term 'potency' in the context of stem cells?
Which type of cells serve as a repair system in adults?
Which type of cells serve as a repair system in adults?
Which of the following cell types is primarily involved in gathering information and controlling body functions?
Which of the following cell types is primarily involved in gathering information and controlling body functions?
Which statement accurately describes the function of macrophages?
Which statement accurately describes the function of macrophages?
What are the three germ layers that stem cells can differentiate into during embryonic development?
What are the three germ layers that stem cells can differentiate into during embryonic development?
Which of the following is NOT a characteristic of stem cells?
Which of the following is NOT a characteristic of stem cells?
What effect does cholesterol have on membrane fluidity at warm temperatures?
What effect does cholesterol have on membrane fluidity at warm temperatures?
Which of the following best describes the structure of glycolipids in the plasma membrane?
Which of the following best describes the structure of glycolipids in the plasma membrane?
What is a key characteristic of integral membrane proteins?
What is a key characteristic of integral membrane proteins?
How do peripheral membrane proteins differ from integral membrane proteins?
How do peripheral membrane proteins differ from integral membrane proteins?
What forms the glycocalyx on the plasma membrane?
What forms the glycocalyx on the plasma membrane?
What role do transmembrane proteins play in the plasma membrane?
What role do transmembrane proteins play in the plasma membrane?
What effect does cold temperature have on membrane fluidity in relation to cholesterol?
What effect does cold temperature have on membrane fluidity in relation to cholesterol?
Which stem cell type is capable of producing only its own specific cell type?
Which stem cell type is capable of producing only its own specific cell type?
Which statement accurately describes the asymmetry of the plasma membrane?
Which statement accurately describes the asymmetry of the plasma membrane?
What is the defining characteristic of oligopotent stem cells?
What is the defining characteristic of oligopotent stem cells?
Which term best describes stem cells that can differentiate into nearly all cell types from the three germ layers?
Which term best describes stem cells that can differentiate into nearly all cell types from the three germ layers?
What is the primary function of mitochondria in human cells?
What is the primary function of mitochondria in human cells?
What structure within the nucleus is primarily involved in ribosome production?
What structure within the nucleus is primarily involved in ribosome production?
What key feature distinguishes unipotent cells from other stem cell types?
What key feature distinguishes unipotent cells from other stem cell types?
What is the primary product of symmetric stem cell division?
What is the primary product of symmetric stem cell division?
Which of the following statements about mitochondria is incorrect?
Which of the following statements about mitochondria is incorrect?
What is contained within the liquid matrix of the mitochondria?
What is contained within the liquid matrix of the mitochondria?
What type of stem cell can only differentiate into a limited number of cell types?
What type of stem cell can only differentiate into a limited number of cell types?
How many chromosomes does the human genome contain?
How many chromosomes does the human genome contain?
Which cell type is at the apex of the hierarchy of hematopoietic differentiation?
Which cell type is at the apex of the hierarchy of hematopoietic differentiation?
What limits the differentiation capacity of pluripotent stem cells compared to totipotent stem cells?
What limits the differentiation capacity of pluripotent stem cells compared to totipotent stem cells?
The primary function of the Kreb’s cycle within mitochondria is to?
The primary function of the Kreb’s cycle within mitochondria is to?
Which cell types would you expect to have a higher density of mitochondria?
Which cell types would you expect to have a higher density of mitochondria?
How are totipotent stem cells produced?
How are totipotent stem cells produced?
What are the primary components forming chromatin in the nucleus?
What are the primary components forming chromatin in the nucleus?
What key feature distinguishes Gram-positive bacteria from Gram-negative bacteria in terms of cell wall composition?
What key feature distinguishes Gram-positive bacteria from Gram-negative bacteria in terms of cell wall composition?
Which statement accurately describes the structural differences between prokaryotic and eukaryotic cells?
Which statement accurately describes the structural differences between prokaryotic and eukaryotic cells?
What is the role of flagella in prokaryotic cells?
What is the role of flagella in prokaryotic cells?
In which way do Gram-negative bacteria respond differently to Gram’s Stain in comparison to Gram-positive bacteria?
In which way do Gram-negative bacteria respond differently to Gram’s Stain in comparison to Gram-positive bacteria?
Which characteristic is typically associated with the structure of eukaryotic cells?
Which characteristic is typically associated with the structure of eukaryotic cells?
What type of cellular structures are common to both prokaryotic and eukaryotic cells?
What type of cellular structures are common to both prokaryotic and eukaryotic cells?
Which component is NOT a primary part of the general structure of a cell?
Which component is NOT a primary part of the general structure of a cell?
What structural role does the cytoskeleton serve in eukaryotic cells?
What structural role does the cytoskeleton serve in eukaryotic cells?
What is the primary structural organization of phospholipids within the plasma membrane?
What is the primary structural organization of phospholipids within the plasma membrane?
How does cholesterol influence the plasma membrane structure?
How does cholesterol influence the plasma membrane structure?
Which statement accurately describes the role of integral proteins in the plasma membrane?
Which statement accurately describes the role of integral proteins in the plasma membrane?
What interaction occurs between the polar region of cholesterol and other membrane components?
What interaction occurs between the polar region of cholesterol and other membrane components?
What is one of the primary functions of carbohydrates attached to the plasma membrane?
What is one of the primary functions of carbohydrates attached to the plasma membrane?
What characteristic does the phospholipid bilayer exhibit due to its amphipathic nature?
What characteristic does the phospholipid bilayer exhibit due to its amphipathic nature?
What is the primary barrier function of the plasma membrane?
What is the primary barrier function of the plasma membrane?
In what manner do membrane proteins influence cellular signaling?
In what manner do membrane proteins influence cellular signaling?
Flashcards
Cell Theory
Cell Theory
The concept that cells are the fundamental building blocks of all living organisms. It states that all living things are made up of cells, that all cells arise from pre-existing cells, and that cells are the smallest unit of life that can carry out all the functions necessary for life.
Stem Cells
Stem Cells
The ability of cells to differentiate into specialized cell types with specific functions, while maintaining the ability to self-renew. Stem cells play a crucial role in development, growth, and tissue repair.
Prokaryotic Cells
Prokaryotic Cells
Simple cells lacking a nucleus and other membrane-bound organelles. Their DNA is located in a region called the nucleoid. They are typically smaller than eukaryotic cells and are found in single-celled organisms like bacteria and archaea.
Eukaryotic Cells
Eukaryotic Cells
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Plant Cells
Plant Cells
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Animal Cells
Animal Cells
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Bacteria
Bacteria
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Plasma Membrane
Plasma Membrane
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What are flagella?
What are flagella?
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What is peptidoglycan?
What is peptidoglycan?
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What is a gram-positive bacteria?
What is a gram-positive bacteria?
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What is a gram-negative bacteria?
What is a gram-negative bacteria?
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What is a eukaryotic cell?
What is a eukaryotic cell?
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What is cytosol?
What is cytosol?
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What is the cytoskeleton?
What is the cytoskeleton?
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What is the plasma membrane?
What is the plasma membrane?
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Totipotent stem cells
Totipotent stem cells
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Pluripotent stem cells
Pluripotent stem cells
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Multipotent stem cells
Multipotent stem cells
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Oligopotent stem cells
Oligopotent stem cells
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Unipotent stem cells
Unipotent stem cells
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Symmetric stem cell division
Symmetric stem cell division
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Asymmetric stem cell division
Asymmetric stem cell division
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Progenitor cell
Progenitor cell
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Differentiated cell
Differentiated cell
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Cell differentiation
Cell differentiation
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Cholesterol's Role in Membrane Fluidity
Cholesterol's Role in Membrane Fluidity
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What are Glycolipids?
What are Glycolipids?
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What are Integral Membrane Proteins?
What are Integral Membrane Proteins?
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What are Transmembrane Proteins?
What are Transmembrane Proteins?
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What are Peripheral Membrane Proteins?
What are Peripheral Membrane Proteins?
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What are Glycoproteins?
What are Glycoproteins?
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What is the Glycocalyx?
What is the Glycocalyx?
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What is the Plasma Membrane's Role?
What is the Plasma Membrane's Role?
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Endoplasmic Reticulum (ER)
Endoplasmic Reticulum (ER)
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Golgi Complex
Golgi Complex
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Lysosomes
Lysosomes
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Peroxisomes
Peroxisomes
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Proteasomes
Proteasomes
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Cytoskeleton
Cytoskeleton
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Cytosol
Cytosol
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What is a centrosome?
What is a centrosome?
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What are cilia and flagella?
What are cilia and flagella?
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What are ribosomes?
What are ribosomes?
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What is the endoplasmic reticulum (ER)?
What is the endoplasmic reticulum (ER)?
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What is rough ER?
What is rough ER?
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What is smooth ER?
What is smooth ER?
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What is a flagellum?
What is a flagellum?
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What are cilia?
What are cilia?
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Extra-embryonic tissues
Extra-embryonic tissues
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Stem cell potency
Stem cell potency
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Zygote
Zygote
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Germ layers
Germ layers
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What are gram-positive bacteria?
What are gram-positive bacteria?
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What are gram-negative bacteria?
What are gram-negative bacteria?
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What are eukaryotic cells?
What are eukaryotic cells?
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What are prokaryotic cells?
What are prokaryotic cells?
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What is the Golgi complex?
What is the Golgi complex?
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How does cholesterol affect membrane fluidity?
How does cholesterol affect membrane fluidity?
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What is the function of the plasma membrane?
What is the function of the plasma membrane?
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What are phospholipids and how are they arranged in the plasma membrane?
What are phospholipids and how are they arranged in the plasma membrane?
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What is the role of cholesterol in the plasma membrane?
What is the role of cholesterol in the plasma membrane?
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What are integral membrane proteins and what are their functions?
What are integral membrane proteins and what are their functions?
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What are peripheral membrane proteins and where are they located?
What are peripheral membrane proteins and where are they located?
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What is the role of carbohydrate groups attached to the plasma membrane?
What is the role of carbohydrate groups attached to the plasma membrane?
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Mitochondria
Mitochondria
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Mitochondrial function
Mitochondrial function
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Nucleus
Nucleus
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Nucleolus
Nucleolus
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Chromatin
Chromatin
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What is the nucleus?
What is the nucleus?
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Protein synthesis directed by DNA
Protein synthesis directed by DNA
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What is the nucleolus?
What is the nucleolus?
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Study Notes
MPharm Programme - Cell Science - Introduction to Cellular Structure 1 & 2
- The course is PHA115, Cellular Structure 1 & 2, taught by Dr. Praveen Bhugra.
- The learning objectives include understanding and explaining in detail cell theory, stem cell theory, differences between prokaryotic and eukaryotic cells, differentiation between plant, animal cells and bacteria, and structure and function of plasma membrane, cytoplasm (cytosol and organelles), and the nucleus.
Cells Theory
- Cells are the building blocks of all plants and animals.
- All cells come from the division of pre-existing cells.
- Cells are the smallest units that perform all vital physiological functions.
- Each cell maintains homeostasis at the cellular level.
Cells in the human body
- The human body is composed of cells.
- An estimated 3.72 x 10¹³ cells exist in the human body.
- A variety of cell types exist within the human body (approximately 200 types).
- Cells contain organelles, which have diverse functions.
Diversity of Human Cells
- Adult humans contain more than 200 different kinds of cells.
- These include nerve, muscle, skin, blood, bone, and cartilage cells.
- Extra-embryonic tissues like the placenta and umbilical cord are essential for embryonic development.
- Specific types of cells have specialized structures and functions.
Stem cells
- Stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (mitosis) to create more stem cells.
- Two primary types of stem cells in mammals are embryonic stem cells and adult stem cells.
- Embryonic stem cells are derived from the inner cell mass of blastocysts.
- Adult stem cells are found in various tissues and function in tissue repair.
- In a developing embryo, stem cells can differentiate into three germ layers (ectoderm, mesoderm, and endoderm).
Stem cells potency
- Totipotent stem cells can differentiate into all types of cell types (including embryonic and extra-embryonic).
- Pluripotent stem cells can differentiate into nearly all cells derived from the three germ layers.
- Multipotent stem cells differentiate into a number of closely related cell types.
- Oligopotent stem cells differentiate into just a few closely related cell types.
- Unipotent stem cells differentiate into just one cell type and have self-renewal abilities.
Stem cell division and differentiation
- Stem cells divide and differentiate to produce progenitor cells and differentiated cells.
- Stem cell division and differentiation can be symmetric or asymmetric and lead to progenitor cell division, followed by terminal differentiation.
Potential uses of stem cells
- Stem cells have potential applications in treating various diseases and conditions (stroke, traumatic brain injury, learning defects, and others), though their therapeutic uses are complex and developing.
Stem cells potency & source developmental
- Totipotent embryonic stem cells are the most versatile.
- Pluripotent embryonic stem cells and induced pluripotent stem cells can generate multiple cell types.
- Multipotent stem cells can generate a range of cells within a family of related cells.
Hierarchy of haemopoietin differentiation
- Stem cells give rise to progenitor cells and mature cells (blood cells).
- Blood cells are categorized into different lineages, with specific progenitor cells leading to distinct blood cell types.
- This process is complex, involving multiple steps and lineage-committed precursors.
Characteristics of all cell types
- All cell types have a surrounding membrane, protoplasm, organelles, and a control center with DNA.
- This leads to prokaryotic and eukaryotic cell categorization.
Characteristics of prokaryotic cells (Bacteria)
- Lack a nucleus and membrane-bound organelles.
- Simplest cell type with a single, circular chromosome.
- The nucleoid region contains DNA.
- Surrounded by a cell membrane and cell wall (peptidoglycan).
- Contain ribosomes for protein synthesis.
- Can be Gram-positive or Gram-negative based on cell wall structure.
Characteristics of eukaryotic cells
- Possess a membrane-bound nucleus.
- More complex than prokaryotic cells, with compartmentalization of functions in organelles.
- Possess a cytoskeleton for support and to maintain cellular structure.
- Include fungi, protozoa, plants, and animals.
An animal cell and A plant cell.
- Both animal and plant cells have a nucleus, cytoplasm, and cell membrane.
- Plant cells have a cell wall, vacuoles, and chloroplasts.
- Animal cells may contain different specialized organelles.
Plant cell
- Plant cells have specific organelles, such as chloroplasts, central vacuole, and a cell wall, that are absent in animal cells.
Animal Cell
- Animal cells are characterized by diverse organelles (e.g., lysosomes, centrioles) that may not be present in plant cells.
Cell Structure
- The three main parts of a cell are the plasma membrane, cytoplasm (cytosol and organelles), and the nucleus.
Generalized View of Cell Structure
- The cell has components that are part of its structure and their functions, including various organelles, and the cytoskeleton.
Plasma membrane
- Main structural element in cells, made of a phospholipid bilayer, cholesterol, and proteins.
- Proteins associated with carbohydrates are glycolipids and glycoproteins.
- The functions of membranes include a barrier between inside and outside of the cell, regulation of transport of materials, and transmitting signals between different areas within and outside the cell.
- Membranes include types of junctions (desmosomes, tight junctions, and gap junctions).
Plasma membrane (Phospholipids)
- Phospholipids are amphipathic molecules that form a bilayer in cell membranes.
Plasma membrane (Cholesterol)
- Cholesterol plays a key role in regulating membrane fluidity at different temperatures.
Plasma membrane (Glycolipids)
- Glycolipids are membrane lipids with carbohydrate groups that face the extracellular fluid.
Plasma Membrane (Protein)
- Integral membrane proteins are tightly associated with the membrane and span the lipid bilayer.
- Peripheral membrane proteins are loosely associated with the membrane.
Plasma Membrane (Junctions)
- Various junction types connect cells (e.g., desmosomes, tight junctions, and gap junctions).
- Integrins are transmembrane proteins that link the membrane to the extracellular matrix.
- Specific proteins associated with junctions help in cell-to-cell interactions.
Desmosomes
- Structures that anchor cells together in tissues that undergo stretching.
Tight junctions
- Link cells tightly, creating an impermeable seal that prevents the leakage of materials between cells.
Gap junctions
- Protein channels that connect the cytosols of adjacent cells, allowing small molecules, ions, and signaling molecules to pass between cells.
Functions of Cell Membranes
- Regulate passage of substances in and out of cells.
- Detect chemical messengers.
- Link adjacent cells.
- Anchor cells to the extracellular matrix.
Cytoplasm (Cytosol)
- The fluid component of the cytoplasm.
- Consists mostly of water plus dissolved substances, including ions, nutrients, and waste products.
Cytoplasm (Cell Organelles)
- Contains various organelles such as cytoskeleton, flagella, cilia, centrioles, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, nucleolus, and nuclear envelope, vesicles, lysosomes, peroxisomes, proteasomes.
Cell organelles (Cytoskeleton)
- Maintains the shape of the cell, positions organelles, and changes cell shape.
- Includes microfilaments, intermediate filaments, and microtubules, which are involved in various cellular processes.
Cell organelles (Centrosome)
- An organizing center for microtubules, playing a key role in cell division.
Cell organelles (Cilia and Flagella)
- Specialized for motion.
- Flagella provide movement for sperm cells; cilia allow for movement of fluids and mucus.
Cell organelles (Ribosomes)
- Sites of protein synthesis.
- Found free in the cytosol or attached to the endoplasmic reticulum.
Cell organelles (Endoplasmic Reticulum (E.R.))
- Network of folded membranes involved in protein synthesis, lipid synthesis, and intracellular transport.
- The ER has rough and smooth regions, each with specific functions.
Cell organelles (Golgi Complex)
- Modifies proteins and lipids and packages them for secretion or transport to other organelles.
Cell organelles (Lysosomes)
- Contain digestive enzymes for breaking down cellular waste.
Cell organelles (Peroxisomes)
- Contain enzymes for detoxification reactions.
Cell organelles (Proteasomes)
- Breakdown of unneeded or damaged proteins.
Cell organelles (Mitochondria)
- The major site of ATP production in cells.
- Contain DNA and ribosomes.
- High concentration in cells with high energy demands (muscle, liver, kidney).
- Contain specialized structures like cristae.
- Involved in cellular respiration.
Nucleus
- The control center of the cell, containing DNA and nucleolus, responsible for regulating protein synthesis, and cell division.
- Contains chromatin, a complex of DNA and associated proteins.
- Involved in gene regulation and transcription.
Comparison of Bacterial, Animal and Plant cells
- A table summarizing the main structural differences across bacterial, animal, and plant cells, indicating the presence or absence of various structures (cell wall, plasma membrane, flagella, DNA, etc.).
Further Reading
- List of textbooks for further study.
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