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Questions and Answers
What is a significant characteristic that distinguishes eukaryotic cells from prokaryotic cells?
What is a significant characteristic that distinguishes eukaryotic cells from prokaryotic cells?
What process describes the conversion of genetic information from DNA to messenger RNA?
What process describes the conversion of genetic information from DNA to messenger RNA?
Which of the following terms refers to the observable characteristics of an organism?
Which of the following terms refers to the observable characteristics of an organism?
What are ribosomes primarily responsible for in a cell?
What are ribosomes primarily responsible for in a cell?
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Which statement regarding chitin is true?
Which statement regarding chitin is true?
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What describes the 'blastula' stage in animals?
What describes the 'blastula' stage in animals?
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What is the role of histones in relation to DNA?
What is the role of histones in relation to DNA?
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Which type of cells are genetically more similar to animals than to plants?
Which type of cells are genetically more similar to animals than to plants?
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What is the primary function of tRNA in protein synthesis?
What is the primary function of tRNA in protein synthesis?
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What distinguishes membrane-bound ribosomes from free ribosomes?
What distinguishes membrane-bound ribosomes from free ribosomes?
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What is the role of the Golgi apparatus in the cell?
What is the role of the Golgi apparatus in the cell?
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What occurs during constitutive secretion?
What occurs during constitutive secretion?
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What is a key feature of regulated secretion?
What is a key feature of regulated secretion?
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How does the smooth endoplasmic reticulum (SER) differ from the rough endoplasmic reticulum (RER)?
How does the smooth endoplasmic reticulum (SER) differ from the rough endoplasmic reticulum (RER)?
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What describes the function of cisternae in the Golgi apparatus?
What describes the function of cisternae in the Golgi apparatus?
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What molecules are typically released through exocytosis?
What molecules are typically released through exocytosis?
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What is the primary function of kinesin in the cell?
What is the primary function of kinesin in the cell?
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Which compartment is located inside the inner membrane of mitochondria?
Which compartment is located inside the inner membrane of mitochondria?
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What do cristae in mitochondria primarily enhance?
What do cristae in mitochondria primarily enhance?
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Which type of junction allows for chemical communication between neighboring cells?
Which type of junction allows for chemical communication between neighboring cells?
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In chloroplasts, what pigment is primarily responsible for synthesizing ATP?
In chloroplasts, what pigment is primarily responsible for synthesizing ATP?
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What is the role of tight junctions in epithelial cells?
What is the role of tight junctions in epithelial cells?
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During which process do mitochondria divide?
During which process do mitochondria divide?
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Which of the following is NOT a component of adherens junctions?
Which of the following is NOT a component of adherens junctions?
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What is the primary function of lysosomes in a cell?
What is the primary function of lysosomes in a cell?
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What are actin filaments primarily responsible for in eukaryotic cells?
What are actin filaments primarily responsible for in eukaryotic cells?
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What distinguishes a lysozyme from a lysosome?
What distinguishes a lysozyme from a lysosome?
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Which cytoskeletal element helps in moving organelles and vesicles within the cell?
Which cytoskeletal element helps in moving organelles and vesicles within the cell?
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What process is primarily facilitated by actin filaments during cell division?
What process is primarily facilitated by actin filaments during cell division?
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What role do motor proteins like kinesin and dynein play in relation to microtubules?
What role do motor proteins like kinesin and dynein play in relation to microtubules?
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What is the diameter of microtubules?
What is the diameter of microtubules?
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Which statement about pseudopodia is true?
Which statement about pseudopodia is true?
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Study Notes
Morphology
- External morphology refers to an organism's size, shape, color, pattern, and outward appearance.
- E. coli and S. cerevisiae are Latin names and should be italicized.
Prokaryotic cells
- Lack an enclosed nucleus or membrane-bound organelles.
- Examples include cocci, bacilli, and vibrio.
Eukaryotic cells
- Include plants, animals, and fungi.
- Possess a nucleus enclosed in an envelope and membrane-bound organelles.
Plant cells
- Most plants are multicellular organisms.
- Obtain energy through photosynthesis.
Fungi cells
- Include yeasts, molds, and mushrooms.
- Heterotrophs with chitin in their cell walls.
- Genetically more similar to animals than plants.
- Heterotrophic organisms consume other plants or animals for energy and nutrients.
- Chitin is a polysaccharide chain.
Animal cells
- Heterotrophic, motile or non-motile.
- Have a blastula stage of embryonic development.
- A blastula is a hollow sphere of cells.
- Eukaryotic cells can be rigid like calcified bone, fluid and flexible like neutrophils, or brick-shaped and fixed like columnar epithelial cells.
Organelles
- Eukaryotes have numerous membrane-bound organelles, while prokaryotes have none.
Nucleus
- The nucleus has entry and exit points called nuclear pores.
- Chromatin is a complex of DNA and protein, where DNA wraps around histones.
Genes to Proteins
- The central dogma describes how genetic information is stored in DNA and converted into a product (protein) within a biological system.
- Transcription: DNA → messenger RNA (mRNA)
- Translation: mRNA → protein
- There are only 4 nucleotides but 20 amino acids, representing a transition from the language of DNA to the language of protein.
- Genotype: the genes an organism possesses.
- Phenotype: the observable characteristics of an organism.
Translation
- Requires 4 nucleotides to translate from mRNA to protein, but there are 20 amino acids.
- More complicated than going from DNA to RNA.
- No equivalent of Watson-Crick base pairing.
Ribosomes
- Molecular machines that help cells make proteins.
- Composed of two parts:
- Small subunit: Reads mRNA instructions for adding amino acids to the growing protein.
- Large subunit: Links amino acids together, forming peptide bonds.
- Amino acids are brought to the ribosome by tRNA.
Types of Ribosomes
- Free ribosomes float freely in the cytosol and can work together in groups called polysomes to make proteins. All ribosomes in bacteria (prokaryotes) are free-floating.
- Membrane-bound ribosomes are attached to the surface of the endoplasmic reticulum (ER) in eukaryotic cells and help make proteins that are sent to specific places.
Endoplasmic Reticulum (ER)
- Rough ER (RER): Has ribosomes attached and helps make proteins that are sent to specific places.
- Smooth ER (SER): Has fewer ribosomes, smaller size, and is involved in lipid, phospholipid, and steroid synthesis.
Golgi Body
- Also known as Golgi apparatus, Golgi complex, or Golgi.
- Cisternae are flat stacks found in certain cell structures like ER or Golgi.
- Involved in processing and transport of proteins and other molecules.
- Proteins arrive from the ER at the cis face and are modified within the cisternae by enzymes.
- Proteins are sorted in the trans Golgi network and sent to the following locations:
- Cell membrane for secretion
- Lysosomes for digestion
Protein Sorting
- The trans Golgi network (TGN) packages proteins into small membrane-bound vesicles for transport.
- Constitutive/exocytotic secretion: Vesicles continuously transport proteins to the plasma membrane, releasing materials like lipids, proteins, and secretory proteins outside the cell.
- Regulated Secretion: Proteins are stored in vesicles until a specific signal triggers their release.
Lysosomes
- Digestive enzymes (lysozymes) are synthesized in the rough endoplasmic reticulum (RER).
- Lysosomes are vesicles containing lysozymes.
- Lysosomes are involved in digesting proteins, waste materials, and harmful substances like bacteria.
- Lysosomal exocytosis can either send proteins to the lysosome for destruction or send lysozymes to digest something outside the cell.
How things move inside the cell
- nm: nanometer (1 millimeter = 1,000 micrometers = 1,000,000 nanometers).
- Pseudopodia: temporary protrusions of the cell's surface for movement and/or feeding.
Cytoskeletal elements
- Protein-based structures within cells that provide support, shape, and organization.
- Involved in movement, division, and maintaining cell integrity.
- Three main types:
- Microtubules
- Intermediate filaments
- Microfilaments
Microtubules
- Made from tubulin, hollow tubes with a diameter of 25 nm.
- Help move organelles and vesicles inside the cell.
- Motor proteins (kinesin and dynein) attach to the outside of the microtubule and transport cargo.
- Kinesin moves towards the cell's edge (positive end), while dynein moves towards the nucleus (negative end).
- Cargo refers to materials like organelles, vesicles, proteins, and other cellular components.
Mitochondria
- Bacilli-shaped, divide by binary fission.
- Produce ATP (energy).
- Have two membranes with an intermembrane space.
- The inner membrane forms cristae, dividing the mitochondrion into two compartments:
- Intermembrane space
- Mitochondrial matrix
- Cristae increase the surface area of the inner membrane, improving the mitochondrion's ability to produce ATP.
- Mitochondria contain their own DNA, separate from the cell's main DNA.
Chloroplast
- Bacilli-shaped, divide by binary fission.
- Synthesize ATP through a photosynthetic pigment called chlorophyll.
- Have two membranes with an intermembrane space.
- Contain their own DNA, separate from the rest of the cell's DNA.
Cell Junctions
- Help hold cells together and allow communication between neighboring cells.
- Permit movement of water and solutes between cells (osmotic regulation).
Gap Junctions
- Facilitate chemical communication between cells.
- Formed by a connexon cylinder consisting of six connexin proteins.
- Allow the movement of smaller structures within cells due to a very small gap between the cells.
Adherens and Desmosomes
- Anchor cells to one another.
- Adherens junctions connect cells via actin-to-actin links through cadherin or integrins.
- Desmosomes connect cells via keratin-to-keratin links through cadherin.
- Hemidesmosomes connect the cytoskeleton of cells to the extracellular matrix via integrins.
Tight Junctions
- Control water and solute movement between epithelial cells.
- Line organs and blood vessels.
- Tighter junctions create tighter barriers, while fewer tight junctions result in leakier barriers.
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Description
This quiz explores the external and internal morphology of prokaryotic and eukaryotic cells. You'll learn about different cell types, including plant, fungi, and animal cells, as well as their unique characteristics. Test your knowledge on the structure and function of these essential biological units.