Podcast
Questions and Answers
What is the primary function of the plasma membrane in a cell?
What is the primary function of the plasma membrane in a cell?
Which of the following statements BEST describes the relationship between cellular structure and function?
Which of the following statements BEST describes the relationship between cellular structure and function?
Which of the following BEST explains the role of the nucleus in a cell?
Which of the following BEST explains the role of the nucleus in a cell?
Which of the following cellular components is NOT considered an organelle?
Which of the following cellular components is NOT considered an organelle?
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What is the significance of the statement "Biochemical activities dictated by subcellular structure" as it relates to the cell?
What is the significance of the statement "Biochemical activities dictated by subcellular structure" as it relates to the cell?
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Based on the characteristics discussed, which of the following is the most likely reason for the diversity of cell types in the human body?
Based on the characteristics discussed, which of the following is the most likely reason for the diversity of cell types in the human body?
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What is the main reason why the cell is considered the fundamental unit of life?
What is the main reason why the cell is considered the fundamental unit of life?
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Which of the following correctly describes the relationship between the cell and the organismal level?
Which of the following correctly describes the relationship between the cell and the organismal level?
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During which phase of mitosis do chromosomes uncoil and revert to chromatin?
During which phase of mitosis do chromosomes uncoil and revert to chromatin?
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Which of the following is NOT a characteristic of a malignant tumor?
Which of the following is NOT a characteristic of a malignant tumor?
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What is the primary function of the mitotic spindle during mitosis?
What is the primary function of the mitotic spindle during mitosis?
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In what way does the protein cyclin contribute to cell division?
In what way does the protein cyclin contribute to cell division?
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What is the primary difference between apoptosis and necrosis?
What is the primary difference between apoptosis and necrosis?
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During which phase of mitosis do sister chromatids line up across the middle of the cell?
During which phase of mitosis do sister chromatids line up across the middle of the cell?
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Which type of microtubules within the mitotic spindle attach to the kinetochore protein on the centromere?
Which type of microtubules within the mitotic spindle attach to the kinetochore protein on the centromere?
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What is the role of actin microfilaments during cytokinesis?
What is the role of actin microfilaments during cytokinesis?
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What is the name of the structure that holds sister chromatids together during prophase?
What is the name of the structure that holds sister chromatids together during prophase?
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What is the primary function of the Golgi complex?
What is the primary function of the Golgi complex?
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Which disorder is associated with the improper secretion of a chloride ion pump protein?
Which disorder is associated with the improper secretion of a chloride ion pump protein?
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What substances do lysosomes primarily digest?
What substances do lysosomes primarily digest?
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How are peroxisomes formed within a cell?
How are peroxisomes formed within a cell?
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Which process follows transcription during protein synthesis?
Which process follows transcription during protein synthesis?
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What is the main function of mitochondria within a cell?
What is the main function of mitochondria within a cell?
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In what phase of the cell cycle does DNA replication occur?
In what phase of the cell cycle does DNA replication occur?
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Which of the following describes the nucleolus?
Which of the following describes the nucleolus?
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What characterizes interphase in somatic cells?
What characterizes interphase in somatic cells?
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What is the consequence of the absence of a specific lysosomal enzyme in Tay-Sachs disorder?
What is the consequence of the absence of a specific lysosomal enzyme in Tay-Sachs disorder?
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Which of the following processes describes the movement of a substance from a region of high concentration to a region of low concentration through a cell membrane, without the help of membrane proteins?
Which of the following processes describes the movement of a substance from a region of high concentration to a region of low concentration through a cell membrane, without the help of membrane proteins?
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What is the primary driving force behind simple diffusion?
What is the primary driving force behind simple diffusion?
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What is the role of transporter proteins in facilitated diffusion?
What is the role of transporter proteins in facilitated diffusion?
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Which of the following factors can influence the rate of simple diffusion?
Which of the following factors can influence the rate of simple diffusion?
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What is the difference between channel-mediated and carrier-mediated facilitated diffusion?
What is the difference between channel-mediated and carrier-mediated facilitated diffusion?
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What is osmosis?
What is osmosis?
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What is the role of aquaporins in osmosis?
What is the role of aquaporins in osmosis?
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What is the difference between a hypotonic solution and a hypertonic solution?
What is the difference between a hypotonic solution and a hypertonic solution?
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What is the difference between primary active transport and secondary active transport?
What is the difference between primary active transport and secondary active transport?
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What is the role of the Na+/K+ ATPase pump in maintaining cell homeostasis?
What is the role of the Na+/K+ ATPase pump in maintaining cell homeostasis?
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What initiates the process of Na+ ions being transported out of the cell?
What initiates the process of Na+ ions being transported out of the cell?
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Which of the following correctly describes the action of Na+ symporter proteins?
Which of the following correctly describes the action of Na+ symporter proteins?
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What is the primary function of receptor-mediated endocytosis?
What is the primary function of receptor-mediated endocytosis?
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What is one consequence of Digitalis on cardiac cells?
What is one consequence of Digitalis on cardiac cells?
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How does endocytosis differ from exocytosis?
How does endocytosis differ from exocytosis?
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Which component of the cytoskeleton is primarily responsible for cell movement?
Which component of the cytoskeleton is primarily responsible for cell movement?
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What is the role of rough ER in a cell?
What is the role of rough ER in a cell?
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Which of the following statements about vesicles is true?
Which of the following statements about vesicles is true?
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Which type of endocytosis allows cells to take in small droplets of extracellular fluid?
Which type of endocytosis allows cells to take in small droplets of extracellular fluid?
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What are centrosomes primarily involved in?
What are centrosomes primarily involved in?
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What defines the difference between antiporters and symporters in transport mechanisms?
What defines the difference between antiporters and symporters in transport mechanisms?
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Which component of the cytoplasm is primarily responsible for important chemical reactions?
Which component of the cytoplasm is primarily responsible for important chemical reactions?
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What characterizes lipids within the cytosol?
What characterizes lipids within the cytosol?
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What is a primary role of membrane-bound ribosomes?
What is a primary role of membrane-bound ribosomes?
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Which of the following is NOT a function of membrane proteins?
Which of the following is NOT a function of membrane proteins?
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Which component of the cell membrane contributes to its fluidity?
Which component of the cell membrane contributes to its fluidity?
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Which type of membrane protein spans the entire cell membrane?
Which type of membrane protein spans the entire cell membrane?
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What is the role of the glycocalyx in the cell membrane?
What is the role of the glycocalyx in the cell membrane?
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Which of the following molecules can easily pass through the lipid bilayer of the cell membrane?
Which of the following molecules can easily pass through the lipid bilayer of the cell membrane?
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Which of these describes the movement of a substance from an area of high concentration to an area of low concentration?
Which of these describes the movement of a substance from an area of high concentration to an area of low concentration?
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Which type of transport process utilizes transporter proteins to move substances across the membrane?
Which type of transport process utilizes transporter proteins to move substances across the membrane?
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What is the role of cholesterol in the cell membrane?
What is the role of cholesterol in the cell membrane?
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Which of the following is NOT a characteristic of passive transport?
Which of the following is NOT a characteristic of passive transport?
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What is the difference between simple diffusion and facilitated diffusion?
What is the difference between simple diffusion and facilitated diffusion?
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What is meant by the term 'selective permeability' in relation to the cell membrane?
What is meant by the term 'selective permeability' in relation to the cell membrane?
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Which of the following is an example of active transport?
Which of the following is an example of active transport?
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Which of the following is NOT a component of the phospholipid bilayer?
Which of the following is NOT a component of the phospholipid bilayer?
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Which type of membrane protein is responsible for recognizing and binding to specific substances?
Which type of membrane protein is responsible for recognizing and binding to specific substances?
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What is the role of 'linker' proteins in the cell membrane?
What is the role of 'linker' proteins in the cell membrane?
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Which of the following processes involves the movement of large particles into the cell by the formation of vesicles?
Which of the following processes involves the movement of large particles into the cell by the formation of vesicles?
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Flashcards
Cell
Cell
The basic living unit of structure and function in all organisms.
Plasma Membrane
Plasma Membrane
A flexible barrier that surrounds the cytoplasm of a cell, regulating what enters and exits.
Cytoplasm
Cytoplasm
The jelly-like substance within the cell membrane that contains organelles and cytosol.
Nucleus
Nucleus
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Cell Growth
Cell Growth
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Mitosis
Mitosis
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Cell Organelles
Cell Organelles
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Cellular Diversity
Cellular Diversity
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Simple Diffusion
Simple Diffusion
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Factors Affecting Diffusion
Factors Affecting Diffusion
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Facilitated Diffusion
Facilitated Diffusion
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Transporter Proteins
Transporter Proteins
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Osmosis
Osmosis
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Isotonic Solution
Isotonic Solution
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Hypotonic Solution
Hypotonic Solution
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Hypertonic Solution
Hypertonic Solution
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Active Transport
Active Transport
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Na+/K+ ATPase Pump
Na+/K+ ATPase Pump
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Na+/K+ Pump
Na+/K+ Pump
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Secondary Active Transport
Secondary Active Transport
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Symporter
Symporter
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Antiporter
Antiporter
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Digitalis
Digitalis
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Exocytosis
Exocytosis
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Endocytosis
Endocytosis
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Phagocytosis
Phagocytosis
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Pinocytosis
Pinocytosis
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Receptor-Mediated Endocytosis
Receptor-Mediated Endocytosis
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Cytoskeleton
Cytoskeleton
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Microfilaments
Microfilaments
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Rough Endoplasmic Reticulum
Rough Endoplasmic Reticulum
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Smooth Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
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Fluid Mosaic Model
Fluid Mosaic Model
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Phospholipid Bilayer
Phospholipid Bilayer
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Glycolipids
Glycolipids
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Cholesterol in Membrane
Cholesterol in Membrane
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Integral Proteins
Integral Proteins
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Peripheral Proteins
Peripheral Proteins
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Passive Transport
Passive Transport
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Selective Permeability
Selective Permeability
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Concentration Gradient
Concentration Gradient
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Electrochemical Gradient
Electrochemical Gradient
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Channel Proteins
Channel Proteins
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Vesicular Transport
Vesicular Transport
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S Phase
S Phase
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Prophase
Prophase
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Metaphase
Metaphase
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Anaphase
Anaphase
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Telophase
Telophase
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Cytokinesis
Cytokinesis
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Cyclin
Cyclin
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Apoptosis
Apoptosis
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Hyperplasia
Hyperplasia
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Carcinogenesis
Carcinogenesis
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Golgi Complex
Golgi Complex
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Cystic Fibrosis
Cystic Fibrosis
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Lysosomes
Lysosomes
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Tay-Sachs Disorder
Tay-Sachs Disorder
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Peroxisomes
Peroxisomes
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Mitochondria
Mitochondria
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Nucleus Function
Nucleus Function
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Transcription
Transcription
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Translation
Translation
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Cell Cycle
Cell Cycle
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Study Notes
Course Information
- Course: PHARM. 181: INTRODUCTION TO HUMAN ANATOMY & PHYSIOLOGY I
- Instructor: Charles Ansah, PhD; FPCPharm; FGHCPharm
- Professor of Pharmacology & Toxicology/Former Dean & Pro Vice-Chancellor
- Location: Room C118 Tackie Building, Department of Pharmacology, Faculty of Pharmacy & Pharmaceutical Sciences, College of Health Sciences, KNUST
Cellular Level of Organization
- Cells are basic, living, structural and functional units of life
- Organismal activity depends on individual and collective activity of cells
- Biochemical activities are dictated by subcellular structure
- General functions of the cell include compartmentalization of biochemical reactions within specialized structures, regulation of inflow & outflow of materials, use of genetic material to direct cell activities, and continuity of life originates from the cell.
Cellular Diversity
- 100 trillion cells in the body, with 200 different types
- Cells vary in size and shape, with different shapes correlating to their specific functions.
Parts of a Cell
- Plasma (cell) membrane
- Cytoplasm (cytosol and organelles)
- Nucleus (chromosomes and genes)
A Typical Cell (Diagram)
- Various organelles are labeled on the diagram.
Plasma Membrane (PM)
- Flexible but sturdy barrier surrounding cytoplasm
- Fluid Mosaic Model:
- Double (bilayer) lipid layer with embedded and dispersed proteins
- Lipids are barriers to polar substances
- Proteins regulate traffic (act as gatekeepers)
- Bilayer consists of phospholipids, cholesterol, and glycolipids
- Glycolipids are lipids with carbohydrates attached
- Phospholipids have both hydrophobic and hydrophilic poles. (hydro phobic tails, hydrophilic heads).
Lipid Bilayer
- Two back-to-back layers of lipid molecules
- Cholesterol and glycolipids scattered among phospholipid molecules
Phospholipids
- Comprise 75% of lipids
- Phospholipid bilayer = 2 parallel layers of molecules
- Each molecule is amphipathic (polar and non-polar regions)
- Polar parts (heads) are hydrophilic and face watery environments on both surfaces
- Nonpolar parts (tails) are hydrophobic and line up in the interior.
Glycolipids
- Comprise 5% of lipids
- Carbohydrate groups form a polar head on the side of the membrane facing the extracellular fluid.
Cholesterol
- Comprises 20% of cell membrane lipids
- Stiff steroid rings and hydrocarbon tail are non-polar and hide in the middle of the cell membrane
Types of Membrane Proteins
- Integral proteins
- Extend into or completely across the cell membrane.
- Transmembrane proteins if they extend completely across the membrane
- Glycoproteins have a sugar portion facing the extracellular fluid to form a glycocalyx; this gives the cell "uniqueness" and creates stickiness to hold it to other cells (or creates a slippery surface).
- Peripheral proteins
- Attached to either inner or outer surface
- Easily removed
Membrane Protein Functions (I)
- Channel Proteins
- Allow specific substances to move through the water-filled pore
- Transporter Proteins
- Change shape to transport specific substances (e.g., amino acids) across the membrane
- Receptor Proteins
- Recognize ligands and change the cell's function (e.g., in the kidney's altering water permeability)
Summary of Membrane Protein Functions (II)
- Cell Identity Marker
- Linker
- Act as an Enzyme
Membrane Fluidity
- Membranes are fluid structures (like an oil layer)
- Self-sealing if punctured
- Membrane molecules can rotate, and move freely
- Difficult for hydrophilic parts to pass through the hydrophobic core of the lipid bilayer
Selective Permeability of the Membrane
- Lipid bilayer is permeable to nonpolar, uncharged molecules (e.g., oxygen, CO2, steroids) and water. Water can flow through gaps in the hydrophobic lipid core.
- Transmembrane proteins act as specific channels for smaller and medium polar, and charged particles.
- Macromolecules cannot directly pass through the membrane, requiring vesicular transport.
Gradients Across the Plasma Membrane
- Membrane maintains differences in concentration of substances (concentration gradient)
- Membrane maintains differences in charged ions (electrical gradient or membrane potential).
- These gradients together create an electrochemical gradient
- Substances move down their concentration gradient and toward the oppositely charged area.
Transport Across the Plasma Membrane
- Passive Processes
- Simple diffusion
- Facilitated diffusion
- Osmosis
- Active Processes
- Active transport
- Vesicular transport
Transport Across Membranes (Summary)
- Mediated transport requires transporter protein
- Non-mediated transport does not use a transporter protein
- Active transport uses ATP to move against the concentration gradient
- Passive transport moves down the concentration gradient using kinetic energy only
- Vesicular transport occurs in small vesicles (exocytosis and endocytosis).
Diffusion Through the Lipid Bilayer
- Important for nutrient absorption and waste excretion of non-polar, hydrophobic molecules like oxygen, carbon dioxide, nitrogen, fatty acids, steroids, small alcohols, ammonia, and fat-soluble vitamins (A, E, D, and K).
Diffusion Through Membrane Channels
- Each channel protein is specific for particular ions (K+, Cl−, Na+, or Ca2+)
- Slower than simple diffusion, but still very rapid.
- Channels may be open all the time or gated (controlled processes).
Passive Processes
- Simple Diffusion
- Facilitated Diffusion
- Osmosis
Simple Diffusion
- Crystal of dye in water, dye diffuses from higher to lower concentration.
- Influenced by: concentration gradient, temperature, mass of the diffusing substance, surface area, diffusion distance.
Principles of Diffusion
- Random mixing of particles due to kinetic energy
- Greater difference in concentration = faster rate of diffusion
- Higher temperature = faster rate of diffusion
- Larger substance = slower rate of diffusion.
- Increase in surface area = faster rate of diffusion
- Longer distance = slower rate of diffusion
- Equilibrium reached when molecules are evenly distributed.
Facilitated Diffusion
- Transmembrane proteins help polar or highly charged solutes pass through the lipid bilayer.
- Two types: channel-mediated and carrier-mediated
Facilitated Diffusion of Glucose
- Glucose binds to a transporter protein.
- Transporter protein changes shape to move glucose into cells.
- Glucose moves only down a concentration gradient.
- Kinase enzymes convert glucose into glucose-6-phosphate, reducing concentration of glucose inside the cell.
- Transporter proteins always bring glucose into the cell.
Channel Mediated Facilitated Diffusion
- Illustrates specifics of how a channel works to move K+ across a membrane (e.g., opening/closing).
Carrier Mediated Facilitated Diffusion
- Illustrates specifics of how a carrier protein works to move glucose across a membrane.
Diffusion: A Comparison
- Compares simple diffusion, channel-mediated, and carrier-mediated facilitated diffusion
Osmosis
- Net movement of water through a selectively permeable membrane from an area of higher water concentration to an area of lower water concentration.
- Occurs if the membrane is permeable to water but not to certain solutes.
- Water diffuses through the lipid bilayer or through aquaporins (transmembrane proteins that act as water channels).
Osmosis of Water Through a Membrane
- Net movement of water from one side of a selectively permeable membrane to the other until hydrostatic pressure (equal or more balanced osmotic pressure) stops net water movement.
Tonicity Effects on RBCs in the Lab
- Isotonic solution: osmotic pressure of cell equals fluid outside the cell; cell volume constant
- Water enters the cell faster than it leaves;
- Water enters and leaves the cell in equal amounts.
Effects of Tonicity on Cell Membranes
- Isotonic solutions (same water concentration inside and outside the cell) result in no net movement of water across the cell membrane.
- Hypotonic solutions (greater water concentration outside the cell) result in hemolysis.
- Hypertonic solutions (lower water concentration outside the cell) result in crenation.
Active Transport
- Movement of polar or charged substances against their concentration gradient.
- Requires energy (e.g., from ATP hydrolysis). Two types:
- Primary active transport (uses ATP directly)
- Secondary active transport (uses an electrochemical gradient).
Primary Active Transport
- ATP energy changes a transporter protein's shape, resulting in a substance being pumped across a plasma membrane against its concentration gradient.
- Na+/K+ ATPase is the most common example (actively pumps Na+ out of the cell and K+ into the cell). Critical to maintaining osmotic pressure across the membrane.
Secondary Active Transport
- Uses energy stored in an ionic concentration gradient to move other substances against their own concentration gradient.
- Na+/K+ pump maintains a low concentration of Na+ inside cells
- The gradient (the Na+ leaking back inside) provides energy for other substances to be transported.
- symporters move substances in the same direction , antiporters move substances in opposite directions
Antiporters and Symporters (Diagram)
Digitalis
- Slows the sodium pump, letting more Na+ accumulate in heart muscle cells, which increases the force of contraction.
Vesicular Transport of Particles
- Exocytosis: vesicles inside the cell fuse with the plasma membrane, releasing their contents (e.g., digestive enzymes, hormones, neurotransmitters, or waste).
- Endocytosis
- Phagocytosis: cell eating (e.g., macrophages engulfing bacteria).
- Pinocytosis: cell drinking (no pseudopods involved; nonselective input of extracellular fluid)
- Receptor-mediated endocytosis: selective input (e.g., receptor for LDL or a virus)
Active Transport in Vesicles: Receptor-mediated Endocytosis (diagram)
- Mechanism for uptake of specific substances
Active Transport in Vesicles: Phagocytosis (diagram)
- Mechanism for cell eating
Active Transport in Vesicles: Bulk-Phase Endocytosis (Pinocytosis) (diagram)
Pinocytosis and Phagocytosis (diagram)
Active Transport in Vesicles: Exocytosis and Transcytosis
- Exocytosis: membrane-enclosed secretory vesicles fuse with the plasma membrane, releasing contents into the extracellular fluid.
- Transcytosis: combined endocytosis and exocytosis to move substances from one side of a cell across and out the other side.
Comparison of Transport Types
- Table comparing passive (diffusion, simple diffusion, facilitated diffusion, osmosis) and active transport (primary, secondary, vesicular) processes
Cell Organelles
- Nonmembranous organelles do not have membranes and are in direct contact with the cytoplasm. Examples include microfilaments, intermediate filaments, microtubules.
- Membranous organelles have one or two lipid bilayer membranes. Examples include ER, Golgi apparatus, lysosomes, peroxisomes, and mitochondria.
Cytosol (Intracellular Fluid)
- 55% of cell volume
- 75-90% water
- Large organic molecules (proteins, carbohydrates, and lipids) suspended by electrical charges
- Small organic molecules (simple sugars) and ions
- Dissolved substances like inclusions (aggregates of a single material), lipid droplets, and glycogen granules.
Cytoskeleton
- Network of protein filaments throughout the cytosol
- Gives cell support, shape, organizes chemical reactions, and allows movement.
Microfilaments, Intermediate Filaments, Microtubules
- Structural components of the cytoskeleton
- Different roles (support, motility, etc)
Centrosome
- Formation site for mitotic spindle and microtubules needed for cell division
- Contains two centrioles arranged at 90 degrees to each other
Ribosomes
- Synthesize proteins
- Free ribosomes synthesize proteins for use inside the cell.
- Membrane-bound ribosomes synthesize proteins needed for the plasma membrane or export.
- Multiple ribosomes can work together as a polyribosome.
Endoplasmic Reticulum (ER)
- Network of membranes forming flattened sacs (cisternae)
- Rough ER: continuous with nuclear envelope, covered in ribosomes; synthesizes, processes, and packages proteins for export; synthesizes proteins locally for use.
- Smooth ER: no attached ribosomes; synthesizes phospholipids, steroid, fats, detoxifies harmful substances (e.g., alcohol).
Golgi Complex
- 3–20 flattened, curved membranous sacs (cisternae)
- Convex side faces ER and concave side faces the cell membrane
- Processes and packages proteins (produced by rough ER).
Packaging By Golgi Complex (diagram)
- Shows process of how proteins are packaged and transferred.
Cystic Fibrosis
- Deadly inherited disorder due to a defect in the chloride ion pump protein secreted by the Golgi or rough ER
- Imbalance in fluid and ion transport across the plasma membrane results in a buildup of thick mucus outside certain cells.
Lysosomes
- Membranous vesicles formed in the Golgi complex
- Contain digestive enzymes
- Digest foreign substances and recycle organelles.
Tay-Sachs Disorder
- Genetic disorder in children of Eastern European descent
- Absence of a single lysosomal enzyme, which normally breaks down a glycolipid.
- Glycolipid accumulates, nerve cells lose functionality, and result in seizures, muscle rigidity, blindness, dementia, death before age 5.
Peroxisomes
- Membranous vesicles smaller than lysosomes (-formed by preexisting ones)
- Contain enzymes to oxidize organic material (eg amino acids and fatty acids, toxic substances like alcohol & formaldehyde)
- Contain catalase that breaks down H2O2
Mitochondria
- Double membrane organelle (outer and inner mitochondrial membrane)
- Inner membrane folds are called cristae
- Central cavity is the matrix; increased surface area for chemical reactions;
- Generate ATP (energy); mitochondria replicate and increase number according to need.
Nucleus
- Large organelle with a double membrane nuclear envelope; continuous with rough ER.
- Perforated by water-filled nuclear pores
- Contains the nucleolus, which is a spherical, dark body within the nucleus (no membrane), site of ribosome assembly. The nucleus contains chromatin—loosely packed DNA in non-dividing cells and chromosomes (tightly packed DNA) in dividing cells.
Function of Nucleus
- DNA is organized into 46 chromosomes.
- Instructions on each chromosome direct the synthesis of specific proteins.
- Non-dividing cells contain nuclear chromatin (loosely packed DNA); dividing cells contain chromosomes (tightly packed DNA before condensing); DNA is copied before condensing.
Protein Synthesis (diagram)
- Transcription and translation steps that show where they are happening within the cell.
Transcription
- DNA sense strand is used as a template to create a messenger RNA (mRNA) strand.
Translation
- mRNA, rRNA, and tRNA work together to make a specific protein.
Normal Cell Division
- Mitosis (somatic cell division) -one parent cell gives rise to 2 identical daughter cells; mitosis= nuclear division; cytokinesis = cytoplasmic division occurring billions of times a day for tissue repair and growth.
- Meiosis (reproductive cell division) -egg and sperm cell production, only in testes and ovaries
The Cell Cycle in Somatic Cells
- Process of cell duplication and division
- Chromosomes must be duplicated to pass genes correctly; genes are passed on correctly via mitosis (4 stages: prophase, metaphase, anaphase, and telophase)
- Cytoplasmic division (cytokinesis) also occurs.
Interphase Stage of Cell Cycle
- Phases of interphase (G1, S, G2)
- G1 = cytoplasm increase (resting stage for non-dividing cells);
- S = DNA replication
- G2 = cytoplasmic growth
Replication of Chromosomes
- Doubling of genetic material (DNA replication) during interphase (S phase)
- DNA unzips; mirror copy is formed (complementary bases) from each old strand (two complete identical DNA molecules are formed).
Stages of Nuclear Division: Mitosis (diagram)
- Shows individual stages of cell division.
Prophase
- Chromatin condenses to form chromosomes
- Nucleolus and nuclear envelope disappear
- Centrosomes move to opposite ends
- Mitotic spindle forms (microtubules)
Metaphase
- Chromatids align at the metaphase plate (middle of cell).
Anaphase
- Sister chromatids separate and move towards opposite poles of the cell
Telophase
- Chromosomes arrive at poles, uncoil to become chromatin
- Nucleoli and nuclear envelopes reform
- Mitotic spindle breaks down
Cytokinesis
- Division of the cytoplasm (and organelles)
- Cleavage furrow forms by actin microfilaments, which indent the plasma membrane
- This division is perpendicular to the mitotic spindle to separate chromosomes correctly
- Results in two daughter cells returning to interphase.
Control of Cell Destiny
- Cell destinies include remaining active, growing/dividing, or dying
- Homeostasis maintains cell multiplication and cell death balance
- The protein cyclin builds up during interphase (and triggers mitosis)
- Programmed cell death (apoptosis)
- Necrosis (cell death from injury or infection)
Aging
- Body's adaptive capacity decreases with age.
- Theories for aging include limited cell divisions, glucose binding to proteins, free radicals, and autoimmune responses
- Aging evidence includes damaged skin, hardened arteries, and stiff joints.
Cancer
- Out of control cell division
- Hyperplasia, benign to malignant tumours
- Causes include carcinogens, x-rays, and viruses
- Mutations in genes that regulate growth and development lead to uncontrolled growth factors
- Carcinogenesis is a multistep process taking years and requiring multiple mutations.
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Test your knowledge on cellular biology with this quiz focusing on the plasma membrane, nucleus, and the relationship between cellular structure and function. It covers fundamental concepts essential for understanding the significance of cells in living organisms.