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Questions and Answers
What is the primary type of bond involved in forming the peptide backbone of a protein?
What is the primary type of bond involved in forming the peptide backbone of a protein?
Which level of protein structure is primarily determined by the sequence of amino acids?
Which level of protein structure is primarily determined by the sequence of amino acids?
What is the main difference between the α-helix and β-strand secondary structures?
What is the main difference between the α-helix and β-strand secondary structures?
Which type of bond is responsible for the stability of the tertiary structure of a protein?
Which type of bond is responsible for the stability of the tertiary structure of a protein?
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What is the significance of a protein domain?
What is the significance of a protein domain?
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How do disulfide bonds contribute to protein stability?
How do disulfide bonds contribute to protein stability?
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Why is it important to memorize all 20 amino acids, their groups, and their three-letter codes?
Why is it important to memorize all 20 amino acids, their groups, and their three-letter codes?
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Which of the following is NOT a type of non-covalent interaction involved in protein structure?
Which of the following is NOT a type of non-covalent interaction involved in protein structure?
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What is the likely location within a cell for a protein with predominantly hydrophobic side chains?
What is the likely location within a cell for a protein with predominantly hydrophobic side chains?
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Which of the following is NOT a property of catalysts?
Which of the following is NOT a property of catalysts?
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Which of the following is an example of an endergonic reaction?
Which of the following is an example of an endergonic reaction?
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Which of the following is TRUE about the Gibbs Free Energy equation (ΔG = ΔH - TΔS)?
Which of the following is TRUE about the Gibbs Free Energy equation (ΔG = ΔH - TΔS)?
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How does ATP facilitate an endergonic reaction?
How does ATP facilitate an endergonic reaction?
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What is the difference between competitive and non-competitive inhibitors?
What is the difference between competitive and non-competitive inhibitors?
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Which of the following is a TRUE statement about the 1st Law of Thermodynamics?
Which of the following is a TRUE statement about the 1st Law of Thermodynamics?
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Which of the following scenarios is an example of a spontaneous process?
Which of the following scenarios is an example of a spontaneous process?
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What structure in prokaryotes contains their genetic information?
What structure in prokaryotes contains their genetic information?
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Which type of prokaryote has a thick cell wall rich in peptidoglycan?
Which type of prokaryote has a thick cell wall rich in peptidoglycan?
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What is the function of the nucleolus within the nucleus of a eukaryotic cell?
What is the function of the nucleolus within the nucleus of a eukaryotic cell?
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Which of the following is true about plasmids in prokaryotes?
Which of the following is true about plasmids in prokaryotes?
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Which advantage is associated with the compartmentalization of organelles in eukaryotic cells?
Which advantage is associated with the compartmentalization of organelles in eukaryotic cells?
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What happens to the crystal violet stain in Gram-negative bacteria during staining?
What happens to the crystal violet stain in Gram-negative bacteria during staining?
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How do Archaea and Eukaryotes relate in the context of evolutionary biology?
How do Archaea and Eukaryotes relate in the context of evolutionary biology?
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What do both Archaea and Bacteria share as their main characteristic?
What do both Archaea and Bacteria share as their main characteristic?
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What was the primary conclusion of Anfinsen’s renaturation experiment?
What was the primary conclusion of Anfinsen’s renaturation experiment?
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Which type of bonds did Anfinsen's experiment specifically target for disruption?
Which type of bonds did Anfinsen's experiment specifically target for disruption?
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What type of protein was used in Anfinsen’s experiment?
What type of protein was used in Anfinsen’s experiment?
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What was the effect of treating Ribonuclease A with urea and β-mercapto ethanol?
What was the effect of treating Ribonuclease A with urea and β-mercapto ethanol?
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Which Nobel Prize did WH Bragg and WL Bragg win for their contributions to understanding protein structure?
Which Nobel Prize did WH Bragg and WL Bragg win for their contributions to understanding protein structure?
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What does the presence of hydrophilic and hydrophobic relationships provide to proteins?
What does the presence of hydrophilic and hydrophobic relationships provide to proteins?
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How many disulfide bonds can form due to the presence of eight cysteines in Ribonuclease A?
How many disulfide bonds can form due to the presence of eight cysteines in Ribonuclease A?
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What structural feature does the protein growth hormone represent?
What structural feature does the protein growth hormone represent?
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What significant biological discovery was revealed in 1953 regarding the formation of biological molecules?
What significant biological discovery was revealed in 1953 regarding the formation of biological molecules?
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Which of the following correctly orders the taxonomical ranks in the classification of life?
Which of the following correctly orders the taxonomical ranks in the classification of life?
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What distinguishes prokaryotic cells from eukaryotic cells?
What distinguishes prokaryotic cells from eukaryotic cells?
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Which statement accurately characterizes Gram-positive and Gram-negative bacteria?
Which statement accurately characterizes Gram-positive and Gram-negative bacteria?
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What role does the chloroplast play in plant cells?
What role does the chloroplast play in plant cells?
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What is the primary function of mitochondria in both plant and animal cells?
What is the primary function of mitochondria in both plant and animal cells?
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What are the domains of life based on the classification system?
What are the domains of life based on the classification system?
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Which of the following represents the correct naming convention for biological classification?
Which of the following represents the correct naming convention for biological classification?
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What was the outcome of disrupting the phage attachments to the bacteria in the experiment?
What was the outcome of disrupting the phage attachments to the bacteria in the experiment?
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Which of the following correctly describes the zwitter-ionic structure of amino acids at pH 7?
Which of the following correctly describes the zwitter-ionic structure of amino acids at pH 7?
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What principle was demonstrated by Christian Anfinsen in his experiments?
What principle was demonstrated by Christian Anfinsen in his experiments?
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How do polar and non-polar side chains interact with water in protein structures?
How do polar and non-polar side chains interact with water in protein structures?
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In water-soluble proteins, where do you typically find non-polar amino acids?
In water-soluble proteins, where do you typically find non-polar amino acids?
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What is the significance of the peptide bond in proteins?
What is the significance of the peptide bond in proteins?
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Which levels of protein structure are described as hierarchical?
Which levels of protein structure are described as hierarchical?
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What differentiates a folded (native) protein from an unfolded (non-native) protein?
What differentiates a folded (native) protein from an unfolded (non-native) protein?
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Flashcards
Miller-Urey Experiment
Miller-Urey Experiment
An experiment simulating early Earth conditions to form amino acids.
Phylogeny
Phylogeny
The study of the evolutionary relationships among organisms.
Linnaean Classification
Linnaean Classification
A hierarchical system for categorizing living organisms.
Three Domains of Life
Three Domains of Life
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Central Dogma of Molecular Biology
Central Dogma of Molecular Biology
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Prokaryotic vs Eukaryotic Cells
Prokaryotic vs Eukaryotic Cells
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Gram-positive vs Gram-negative Bacteria
Gram-positive vs Gram-negative Bacteria
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Chloroplast vs Mitochondria
Chloroplast vs Mitochondria
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Prokaryotes
Prokaryotes
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Coccus
Coccus
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Bacillus
Bacillus
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Plasmids
Plasmids
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Conjugation
Conjugation
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Gram-positive
Gram-positive
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Eukaryotes
Eukaryotes
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Nucleus
Nucleus
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Amino Acid Structure
Amino Acid Structure
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20 Amino Acids
20 Amino Acids
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Chemical Classes of Amino Acids
Chemical Classes of Amino Acids
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Zwitter-ionic Structure
Zwitter-ionic Structure
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Peptide Bond
Peptide Bond
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Levels of Protein Structure
Levels of Protein Structure
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Native vs Non-Native Protein
Native vs Non-Native Protein
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Protein Folding Interactions
Protein Folding Interactions
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Covalent Disulphide Bonds
Covalent Disulphide Bonds
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Non-Covalent Bonds
Non-Covalent Bonds
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Protein Domains
Protein Domains
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Primary Structure of Proteins
Primary Structure of Proteins
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Secondary Structure of Proteins
Secondary Structure of Proteins
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Tertiary Structure of Proteins
Tertiary Structure of Proteins
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Protein Folding
Protein Folding
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Anfinsen’s Experiment
Anfinsen’s Experiment
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Ribonuclease A
Ribonuclease A
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Denaturation
Denaturation
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Hydrophobic Interactions
Hydrophobic Interactions
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X-ray Diffraction
X-ray Diffraction
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Secondary Structure
Secondary Structure
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Disulfide Bonds
Disulfide Bonds
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Hydrophilic Sidechains
Hydrophilic Sidechains
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Hydrophobic Sidechains
Hydrophobic Sidechains
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1st Law of Thermodynamics
1st Law of Thermodynamics
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Gibbs Free Energy Equation
Gibbs Free Energy Equation
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Kinetic Energy
Kinetic Energy
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Potential Energy
Potential Energy
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Endergonic Reaction
Endergonic Reaction
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Study Notes
Ideas and Understanding: Week One
- Living organisms exhibit homeostasis, organization, metabolism, reproduction, and evolution.
- Life on Earth evolved from chemical processes over vast time scales.
- Early Earth conditions included high temperatures, CO2, N2, H2O vapor, H2, H2S, NH3, and CH4.
- Key characteristics of life include homeostasis, organization, metabolism, and reproduction.
What is Life?
- There's no strict definition of life, but all living things share certain characteristics:
- Homeostasis: maintaining internal stability (e.g., temperature, pH)
- Organization: well-structured, often symmetrical cellular organization
- Metabolism: energy transformation within the organism
- Evolution (not an absolute necessity but observed in nature)
- Reproduction: creating offspring
How Did Life Begin?
- The Earth is approximately 4.5 billion years old.
- Early Earth conditions were different from present day (high temperatures, no free oxygen, etc)
- Possible energy sources included geothermal heat, UV radiation, volcanism, lightning, and meteorites.
- Stanley Miller's experiment simulated early Earth conditions and produced amino acids, proving simple biological molecules could form from inorganic material.
The Prokaryotic Cell
- Prokaryotes are single-celled organisms, lacking a nucleus and membrane-bound organelles.
- They come in various shapes (coccus, bacillus, spiral).
- Genetic material is a single circular chromosome in a nucleoid region.
- Plasmids are small, extrachromosomal DNA molecules, that can be transferred between bacteria.
- Cell walls, often containing peptidoglycan, define the bacterial structure.
- Can be classified as Gram positive or Gram negative based on cell wall characteristics and staining.
The Eukaryotic Cell
- Eukaryotes are organisms with membrane-bound organelles and a nucleus.
- Organelles like nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, mitochondria, and chloroplasts are key features.
- These organelles carry out specific tasks, leading to improved efficiency within the cell.
- Endosymbiotic theory proposes that mitochondria and chloroplasts evolved from free-living prokaryotes.
Ideas and Understanding: Week Two
- The central dogma of biology describes the flow of information from DNA to RNA to protein.
- Differentiate prokaryotic and eukaryotic cells.
- Identify Gram-positive and Gram-negative bacteria through Gram staining.
- Distinguish between animal and plant cells.
- Highlight the endomembrane system's structure and function (nucleus, endoplasmic reticulum, Golgi, lysosomes, vacuoles).
- Contrast mitochondria and chloroplasts in function (respiration and photosynthesis, respectively).
- Outline the endosymbiotic theory.
Ideas and Understanding: Week Three
- Identify the functional groups in molecules and their influence on properties.
- Define chiral centers and stereoisomers.
- Understand the chemical structures of the four major biological molecules (carbohydrates, fats, proteins, and nucleic acids).
- Explain how to synthesize large polymers from monomers (dehydration reactions).
- Differentiate between starch and cellulose and their evolutionary significance.
- Explain the functions of carbohydrates.
- Understand fats and lipids and their types.
- Describe the importance of lipids in biological systems.
Ideas and Understanding: Week Four
- Identify the structure of nucleotides and nucleic acids.
- Understand the base pairing rules in DNA (A-T, G-C).
- Understand the double helix structure of DNA.
- Describe the Hershey-Chase experiment and its significance.
- Understand gel electrophoresis and the visualization of DNA.
Ideas and Understanding: Week Five
- Define and describe amino acid structure.
- Identify the 20 amino acids and their properties.
- Describe the hierarchical levels of protein structure (primary, secondary, tertiary, and quaternary).
- Explain protein folding and Anfinsen's experiment.
- Explain that the primary structure of a protein determines its three-dimensional structure and thus its function.
Ideas and Understanding: Week Six
- Describe the energy exchange in chemical reactions (endergonic, exergonic, Gibbs free energy).
- Explain how ATP is used in coupled reactions.
- Explain the role of enzymes as catalysts and factors affecting enzyme activity.
- Describe factors impacting enzyme activity.
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Description
Explore the fundamental characteristics of life in this quiz based on the first chapter of your biology curriculum. Gain insights into homeostasis, organization, metabolism, reproduction, and the evolutionary processes that have shaped life on Earth. Test your understanding of how life originated and the conditions of early Earth.