Biology Chapter 1: Understanding Life
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Questions and Answers

What is the primary type of bond involved in forming the peptide backbone of a protein?

  • Hydrogen bonds
  • Van der Waals interactions
  • Covalent bonds (correct)
  • Ionic bonds
  • Which level of protein structure is primarily determined by the sequence of amino acids?

  • Quaternary structure
  • Primary structure (correct)
  • Tertiary structure
  • Secondary structure
  • What is the main difference between the α-helix and β-strand secondary structures?

  • The α-helix is more flexible than the β-strand.
  • The α-helix is formed by hydrogen bonds between amino acid side chains, while the β-strand is formed by hydrogen bonds between the backbone.
  • The α-helix contains only hydrophobic amino acids, while the β-strand contains both hydrophobic and hydrophilic amino acids.
  • The α-helix is a coiled structure, while the β-strand is a pleated or folded structure. (correct)
  • Which type of bond is responsible for the stability of the tertiary structure of a protein?

    <p>All of the above (D)</p> Signup and view all the answers

    What is the significance of a protein domain?

    <p>All of the above (D)</p> Signup and view all the answers

    How do disulfide bonds contribute to protein stability?

    <p>All of the above (D)</p> Signup and view all the answers

    Why is it important to memorize all 20 amino acids, their groups, and their three-letter codes?

    <p>All of the above (D)</p> Signup and view all the answers

    Which of the following is NOT a type of non-covalent interaction involved in protein structure?

    <p>Disulfide bonds (C)</p> Signup and view all the answers

    What is the likely location within a cell for a protein with predominantly hydrophobic side chains?

    <p>The cell membrane (B)</p> Signup and view all the answers

    Which of the following is NOT a property of catalysts?

    <p>They change the equilibrium constant of a reaction (C)</p> Signup and view all the answers

    Which of the following is an example of an endergonic reaction?

    <p>The synthesis of ATP from ADP and inorganic phosphate (B)</p> Signup and view all the answers

    Which of the following is TRUE about the Gibbs Free Energy equation (ΔG = ΔH - TΔS)?

    <p>A positive TΔS indicates a reaction that requires energy input (C)</p> Signup and view all the answers

    How does ATP facilitate an endergonic reaction?

    <p>By coupling the endergonic reaction with an exergonic reaction (B)</p> Signup and view all the answers

    What is the difference between competitive and non-competitive inhibitors?

    <p>Competitive inhibitors bind to the active site of the enzyme, while non-competitive inhibitors bind to a different site. (A)</p> Signup and view all the answers

    Which of the following is a TRUE statement about the 1st Law of Thermodynamics?

    <p>Energy can be transferred from one form to another, but it cannot be created or destroyed. (D)</p> Signup and view all the answers

    Which of the following scenarios is an example of a spontaneous process?

    <p>Ice melting at room temperature (A), A hot cup of coffee getting colder (B)</p> Signup and view all the answers

    What structure in prokaryotes contains their genetic information?

    <p>Nucleoid region (A)</p> Signup and view all the answers

    Which type of prokaryote has a thick cell wall rich in peptidoglycan?

    <p>Gram-positive (B)</p> Signup and view all the answers

    What is the function of the nucleolus within the nucleus of a eukaryotic cell?

    <p>To synthesise RNA molecules (B)</p> Signup and view all the answers

    Which of the following is true about plasmids in prokaryotes?

    <p>They are independent of chromosomal DNA. (C)</p> Signup and view all the answers

    Which advantage is associated with the compartmentalization of organelles in eukaryotic cells?

    <p>Separation of incompatible chemical reactions (D)</p> Signup and view all the answers

    What happens to the crystal violet stain in Gram-negative bacteria during staining?

    <p>It is removed by the alcohol rinse. (D)</p> Signup and view all the answers

    How do Archaea and Eukaryotes relate in the context of evolutionary biology?

    <p>Archaea are more closely related to Eukaryotes than to Bacteria. (A)</p> Signup and view all the answers

    What do both Archaea and Bacteria share as their main characteristic?

    <p>Lack of membrane-bound organelles (D)</p> Signup and view all the answers

    What was the primary conclusion of Anfinsen’s renaturation experiment?

    <p>The primary structure of a protein dictates its shape and function. (C)</p> Signup and view all the answers

    Which type of bonds did Anfinsen's experiment specifically target for disruption?

    <p>Disulfide bonds and hydrogen bonds. (B)</p> Signup and view all the answers

    What type of protein was used in Anfinsen’s experiment?

    <p>Ribonuclease A. (C)</p> Signup and view all the answers

    What was the effect of treating Ribonuclease A with urea and β-mercapto ethanol?

    <p>The protein entered a denatured state. (B)</p> Signup and view all the answers

    Which Nobel Prize did WH Bragg and WL Bragg win for their contributions to understanding protein structure?

    <p>Nobel Prize in Physics. (D)</p> Signup and view all the answers

    What does the presence of hydrophilic and hydrophobic relationships provide to proteins?

    <p>They drive the folding process of proteins. (B)</p> Signup and view all the answers

    How many disulfide bonds can form due to the presence of eight cysteines in Ribonuclease A?

    <p>4 disulfide bonds. (A)</p> Signup and view all the answers

    What structural feature does the protein growth hormone represent?

    <p>All alpha-helix structure. (B)</p> Signup and view all the answers

    What significant biological discovery was revealed in 1953 regarding the formation of biological molecules?

    <p>Simple biological molecules can be created from inorganic materials. (D)</p> Signup and view all the answers

    Which of the following correctly orders the taxonomical ranks in the classification of life?

    <p>Kingdom, Phylum, Class, Order, Family, Genus, Species (A)</p> Signup and view all the answers

    What distinguishes prokaryotic cells from eukaryotic cells?

    <p>Eukaryotic cells have a defined nucleus, while prokaryotic cells do not. (C)</p> Signup and view all the answers

    Which statement accurately characterizes Gram-positive and Gram-negative bacteria?

    <p>Gram-positive bacteria retain the crystal violet stain used in gram staining. (B)</p> Signup and view all the answers

    What role does the chloroplast play in plant cells?

    <p>It aids in photosynthesis by converting light energy into chemical energy. (C)</p> Signup and view all the answers

    What is the primary function of mitochondria in both plant and animal cells?

    <p>They produce ATP through cellular respiration. (A)</p> Signup and view all the answers

    What are the domains of life based on the classification system?

    <p>Bacteria, Archaea, Eukarya (C)</p> Signup and view all the answers

    Which of the following represents the correct naming convention for biological classification?

    <p>Genus first, then Species, with the Genus capitalized and both italicized. (B)</p> Signup and view all the answers

    What was the outcome of disrupting the phage attachments to the bacteria in the experiment?

    <p>Bacteria were separated from the phages. (D)</p> Signup and view all the answers

    Which of the following correctly describes the zwitter-ionic structure of amino acids at pH 7?

    <p>They possess both positive and negative charges. (D)</p> Signup and view all the answers

    What principle was demonstrated by Christian Anfinsen in his experiments?

    <p>The primary sequence of amino acids determines the 3D structure and function of a protein. (C)</p> Signup and view all the answers

    How do polar and non-polar side chains interact with water in protein structures?

    <p>Polar side chains interact with water while non-polar side chains avoid it. (D)</p> Signup and view all the answers

    In water-soluble proteins, where do you typically find non-polar amino acids?

    <p>Forming the hydrophobic core of the protein. (D)</p> Signup and view all the answers

    What is the significance of the peptide bond in proteins?

    <p>It connects amino acids and maintains a planar structure. (B)</p> Signup and view all the answers

    Which levels of protein structure are described as hierarchical?

    <p>All four levels: primary, secondary, tertiary, and quaternary. (A)</p> Signup and view all the answers

    What differentiates a folded (native) protein from an unfolded (non-native) protein?

    <p>Only native proteins have biological activity. (A)</p> Signup and view all the answers

    Flashcards

    Miller-Urey Experiment

    An experiment simulating early Earth conditions to form amino acids.

    Phylogeny

    The study of the evolutionary relationships among organisms.

    Linnaean Classification

    A hierarchical system for categorizing living organisms.

    Three Domains of Life

    All life is classified into Bacteria, Archaea, and Eukarya.

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    Central Dogma of Molecular Biology

    The flow of genetic information from DNA to RNA to protein.

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    Prokaryotic vs Eukaryotic Cells

    Prokaryotes lack a nucleus; Eukaryotes have a nucleus.

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    Gram-positive vs Gram-negative Bacteria

    Differentiated by cell wall structure and staining properties.

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    Chloroplast vs Mitochondria

    Chloroplasts are for photosynthesis; mitochondria for respiration.

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    Prokaryotes

    Simple organisms without a defined nucleus, including Archaea and Bacteria.

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    Coccus

    A shape of prokaryotes that is spherical in form.

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    Bacillus

    A rod-shaped form of prokaryotes.

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    Plasmids

    Small circular DNA molecules that replicate independently from the chromosome.

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    Conjugation

    A process where genetic information is transferred between bacteria.

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    Gram-positive

    Bacteria with a thick peptidoglycan cell wall that retains crystal violet dye.

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    Eukaryotes

    Organisms with a defined nucleus and larger, more complex cells than prokaryotes.

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    Nucleus

    The membrane-bound structure containing eukaryotic chromosomes and RNA synthesis.

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    Amino Acid Structure

    Amino acids consist of a backbone and a side chain that defines their properties.

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    20 Amino Acids

    There are 20 amino acids, each with a unique 3-letter code and chemical class.

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    Chemical Classes of Amino Acids

    Amino acids are classified into four chemical classes based on their side chain properties.

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    Zwitter-ionic Structure

    At pH 7, amino acids can exist in a zwitter-ionic form with both positive and negative charges.

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    Peptide Bond

    A peptide bond is the link between amino acids in a protein polymer.

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    Levels of Protein Structure

    Proteins have four structural levels: primary, secondary, tertiary, and quaternary.

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    Native vs Non-Native Protein

    Folded (native) proteins have a specific shape, while unfolded (non-native) proteins do not.

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    Protein Folding Interactions

    Proteins fold based on interactions between polar and non-polar side chains.

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    Covalent Disulphide Bonds

    Strong bonds that stabilize protein structure by connecting cysteine residues.

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    Non-Covalent Bonds

    Weaker interactions that help maintain protein shape (e.g., hydrogen bonds, ionic interactions).

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    Protein Domains

    Sections of a protein that can fold independently and function separately.

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    Primary Structure of Proteins

    The unique sequence of amino acids in a protein chain.

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    Secondary Structure of Proteins

    Regular structures like alpha-helix or beta-strand formed by hydrogen bonding.

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    Tertiary Structure of Proteins

    The 3D shape formed by interactions between R-groups in a polypeptide chain.

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    Protein Folding

    The process where a protein assumes its functional three-dimensional shape.

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    Anfinsen’s Experiment

    An experiment demonstrating that primary amino acid sequence determines protein structure.

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    Ribonuclease A

    A protein used in Anfinsen's experiment to show how primary structure determines function.

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    Denaturation

    The process in which a protein loses its native structure due to external stress.

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    Hydrophobic Interactions

    Interactions that drive non-polar side chains to avoid water, promoting protein folding.

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    X-ray Diffraction

    A technique used to visualize the three-dimensional structure of proteins.

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    Secondary Structure

    Regions of proteins that fold into alpha-helices or beta-strands.

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    Disulfide Bonds

    Covalent bonds that stabilize protein structure, formed between cysteine residues.

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    Hydrophilic Sidechains

    Sidechains of proteins that attract water, found on the outside of soluble proteins like growth hormone.

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    Hydrophobic Sidechains

    Sidechains of proteins that repel water, found on the outside of non-soluble proteins like porin.

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    1st Law of Thermodynamics

    Energy cannot be created or destroyed, only transformed.

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    Gibbs Free Energy Equation

    ΔG = ΔH - TΔS; measures energy available to do work in a reaction.

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    Kinetic Energy

    Energy of an object due to its motion.

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    Potential Energy

    Stored energy based on position or state of matter.

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    Endergonic Reaction

    Reactions that require energy input; ΔG is positive.

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    Study Notes

    Ideas and Understanding: Week One

    • Living organisms exhibit homeostasis, organization, metabolism, reproduction, and evolution.
    • Life on Earth evolved from chemical processes over vast time scales.
    • Early Earth conditions included high temperatures, CO2, N2, H2O vapor, H2, H2S, NH3, and CH4.
    • Key characteristics of life include homeostasis, organization, metabolism, and reproduction.

    What is Life?

    • There's no strict definition of life, but all living things share certain characteristics:
      • Homeostasis: maintaining internal stability (e.g., temperature, pH)
      • Organization: well-structured, often symmetrical cellular organization
      • Metabolism: energy transformation within the organism
      • Evolution (not an absolute necessity but observed in nature)
      • Reproduction: creating offspring

    How Did Life Begin?

    • The Earth is approximately 4.5 billion years old.
    • Early Earth conditions were different from present day (high temperatures, no free oxygen, etc)
    • Possible energy sources included geothermal heat, UV radiation, volcanism, lightning, and meteorites.
    • Stanley Miller's experiment simulated early Earth conditions and produced amino acids, proving simple biological molecules could form from inorganic material.

    The Prokaryotic Cell

    • Prokaryotes are single-celled organisms, lacking a nucleus and membrane-bound organelles.
    • They come in various shapes (coccus, bacillus, spiral).
    • Genetic material is a single circular chromosome in a nucleoid region.
    • Plasmids are small, extrachromosomal DNA molecules, that can be transferred between bacteria.
    • Cell walls, often containing peptidoglycan, define the bacterial structure.
    • Can be classified as Gram positive or Gram negative based on cell wall characteristics and staining.

    The Eukaryotic Cell

    • Eukaryotes are organisms with membrane-bound organelles and a nucleus.
    • Organelles like nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, mitochondria, and chloroplasts are key features.
    • These organelles carry out specific tasks, leading to improved efficiency within the cell.
    • Endosymbiotic theory proposes that mitochondria and chloroplasts evolved from free-living prokaryotes.

    Ideas and Understanding: Week Two

    • The central dogma of biology describes the flow of information from DNA to RNA to protein.
    • Differentiate prokaryotic and eukaryotic cells.
    • Identify Gram-positive and Gram-negative bacteria through Gram staining.
    • Distinguish between animal and plant cells.
    • Highlight the endomembrane system's structure and function (nucleus, endoplasmic reticulum, Golgi, lysosomes, vacuoles).
    • Contrast mitochondria and chloroplasts in function (respiration and photosynthesis, respectively).
    • Outline the endosymbiotic theory.

    Ideas and Understanding: Week Three

    • Identify the functional groups in molecules and their influence on properties.
    • Define chiral centers and stereoisomers.
    • Understand the chemical structures of the four major biological molecules (carbohydrates, fats, proteins, and nucleic acids).
    • Explain how to synthesize large polymers from monomers (dehydration reactions).
    • Differentiate between starch and cellulose and their evolutionary significance.
    • Explain the functions of carbohydrates.
    • Understand fats and lipids and their types.
    • Describe the importance of lipids in biological systems.

    Ideas and Understanding: Week Four

    • Identify the structure of nucleotides and nucleic acids.
    • Understand the base pairing rules in DNA (A-T, G-C).
    • Understand the double helix structure of DNA.
    • Describe the Hershey-Chase experiment and its significance.
    • Understand gel electrophoresis and the visualization of DNA.

    Ideas and Understanding: Week Five

    • Define and describe amino acid structure.
    • Identify the 20 amino acids and their properties.
    • Describe the hierarchical levels of protein structure (primary, secondary, tertiary, and quaternary).
    • Explain protein folding and Anfinsen's experiment.
    • Explain that the primary structure of a protein determines its three-dimensional structure and thus its function.

    Ideas and Understanding: Week Six

    • Describe the energy exchange in chemical reactions (endergonic, exergonic, Gibbs free energy).
    • Explain how ATP is used in coupled reactions.
    • Explain the role of enzymes as catalysts and factors affecting enzyme activity.
    • Describe factors impacting enzyme activity.

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    Explore the fundamental characteristics of life in this quiz based on the first chapter of your biology curriculum. Gain insights into homeostasis, organization, metabolism, reproduction, and the evolutionary processes that have shaped life on Earth. Test your understanding of how life originated and the conditions of early Earth.

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