Biological Membrane Overview
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Questions and Answers

What is the primary location of glycoproteins and glycolipids within eukaryotic cells?

  • Nucleus
  • Cytoplasm
  • Plasma membrane (correct)
  • Golgi apparatus
  • Which of the following is NOT a function of glycoproteins in the cell membrane?

  • Protein synthesis (correct)
  • Receptor activity
  • Immune response
  • Cell-cell adhesion
  • What is the main difference between N-linked and O-linked glycosylation?

  • The type of sugar molecule attached to the protein
  • The location of the carbohydrate attachment on the protein (correct)
  • The length of the carbohydrate chain
  • The function of the glycoprotein
  • Which of the following is NOT a component of the extracellular matrix (ECM)?

    <p>Connexons (A)</p> Signup and view all the answers

    Which type of cell junction allows for direct communication between adjacent cells?

    <p>Gap junctions (C)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of lipid rafts?

    <p>They are composed of unsaturated fatty acids. (A)</p> Signup and view all the answers

    What is the main function of sterols in biological membranes?

    <p>To maintain membrane fluidity over a range of temperatures. (D)</p> Signup and view all the answers

    Which type of protein is NOT directly embedded within the membrane?

    <p>Peripheral proteins (B)</p> Signup and view all the answers

    Which scientist(s) proposed the "sandwich" model of the cell membrane, consisting of a lipid bilayer sandwiched between protein sheets?

    <p>Danielli and Davson (C)</p> Signup and view all the answers

    Which of the following is NOT a function of membrane proteins?

    <p>Synthesis of proteins within the cell. (C)</p> Signup and view all the answers

    What is the primary function of cholesterol in the cell membrane?

    <p>Providing structural support and stability (B)</p> Signup and view all the answers

    Which of these is NOT a function of the cell membrane?

    <p>Generating energy for the cell through ATP synthesis (D)</p> Signup and view all the answers

    What is the role of cholesterol in lipid rafts?

    <p>Cholesterol helps to stabilize the lipid rafts and maintain their structure. (D)</p> Signup and view all the answers

    What is the significance of the 'fluid mosaic' model proposed by Singer and Nicolson?

    <p>It highlights the dynamic and flexible nature of the membrane with moving components (C)</p> Signup and view all the answers

    What is the approximate thickness of a cell membrane?

    <p>7-10 nm (D)</p> Signup and view all the answers

    What is the primary characteristic of integral proteins that distinguishes them from peripheral proteins?

    <p>They are attached to the membrane by covalent bonds. (B)</p> Signup and view all the answers

    What type of membrane protein is characterized by its covalent bond to saturated fatty acids or an isoprenyl group?

    <p>Lipid-anchored membrane proteins (B)</p> Signup and view all the answers

    Which of the following types of bonds is the strongest when disrupting molecular forces in proteins?

    <p>Covalent bonds (C)</p> Signup and view all the answers

    Which property of membrane proteins is influenced by temperature changes?

    <p>Lateral movement of proteins (A)</p> Signup and view all the answers

    What structural feature do transmembrane segments of integral proteins often have?

    <p>Hydrophobic amino acid sequences (B)</p> Signup and view all the answers

    How do peripheral proteins primarily associate with membranes?

    <p>With the use of weak electrostatic forces and hydrogen bonds (D)</p> Signup and view all the answers

    In what way do membrane protein complexes contribute to cellular functions?

    <p>Via proton pumping and electron transport (A)</p> Signup and view all the answers

    Which of the following statements is true about protein movements within the membrane?

    <p>They are generally more restricted than lipid movements. (B)</p> Signup and view all the answers

    What is the effect of increasing the concentration of saturated fatty acids in a membrane?

    <p>Decreased membrane fluidity (C)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of phospholipids?

    <p>They are the primary component of all cellular membranes (D)</p> Signup and view all the answers

    How does cholesterol affect membrane fluidity?

    <p>Cholesterol increases fluidity at low temperatures and decreases fluidity at high temperatures (C)</p> Signup and view all the answers

    What is the role of lipid rafts in the cell membrane?

    <p>They provide a platform for specific proteins to interact and function (C)</p> Signup and view all the answers

    What is the primary location of cholesterol in a eukaryotic cell?

    <p>Plasma membrane (A)</p> Signup and view all the answers

    Which of the following statements about the physical characteristics of lipid bilayers is TRUE?

    <p>They can be easily deformed by small forces (B)</p> Signup and view all the answers

    What is the effect of increasing temperature on the membrane fluidity?

    <p>Increased membrane fluidity (A)</p> Signup and view all the answers

    Which of the following is NOT a feature of lipid bilayers?

    <p>High permeability to large molecules (C)</p> Signup and view all the answers

    Which of the following factors influences the fluidity of a lipid bilayer?

    <p>All of the above (D)</p> Signup and view all the answers

    Why is it important for biological membranes to be fluid?

    <p>To allow for membrane transport and cell signaling (D)</p> Signup and view all the answers

    Flashcards

    Tm (Transition Temperature)

    The temperature at which membrane fluidity changes significantly.

    Lipid Rafts

    Dynamic microdomains in membranes, thicker and less fluid, rich in cholesterol and saturated lipids.

    Function of Lipid Rafts

    Involved in cholesterol transport, endocytosis, signal transduction, and sequestering proteins.

    Integral Membrane Proteins

    Proteins that are permanently attached and span across the membrane, involved in transport and signaling.

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    Peripheral Membrane Proteins

    Proteins that attach loosely to the membrane's surface, often by electrostatic interactions.

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    Glycoproteins

    Proteins with carbohydrate side chains added through glycosylation.

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    N-linked glycosylation

    Attachment of carbohydrates to proteins via nitrogen (amino group).

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    O-linked glycosylation

    Attachment of carbohydrates to proteins via oxygen (hydroxyl group).

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    Glycolipids

    Lipids with carbohydrate chains mainly on the cell's exterior.

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    Cell adhesion

    Processes that allow cells to interact and communicate, involving ECM and cell junctions.

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    Phospholipids

    Basic components of all biological membranes, amphipathic in nature.

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    Amphipathic molecules

    Molecules with both hydrophilic and hydrophobic parts.

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    Phospholipid bilayer

    Forms a stable barrier between two aqueous environments due to phospholipid arrangement.

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    Fluidity of membranes

    Refers to how easily lipids and proteins move within the bilayer.

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    Saturation of fatty acids

    The degree to which fatty acid chains contain double bonds that affect membrane fluidity.

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    Cholesterol

    A lipid that stabilizes cell membranes and alters their fluidity.

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    Temperature effect on fluidity

    Changes in temperature affect the fluidity of the lipid bilayer.

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    Self-healing of membranes

    Ability of lipid bilayers to spontaneously reseal after damage.

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    Cell Membrane

    A biological barrier that separates the interior of the cell from the external environment, facilitating selective transport and communication.

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    Fluid Mosaic Theory

    A model describing the cell membrane as a dynamic structure with a mix of lipids and proteins, allowing movement and fluidity.

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    Membrane Lipids

    Fat molecules, mainly phospholipids, that form a bilayer in the cell membrane providing structure and stability.

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    Integral Proteins

    Proteins that span the entire membrane, playing key roles in transport and signaling.

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    Transduction of signals

    The process of converting external signals into cellular responses through energy input.

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    Covalent bond disruption

    Energy required to break covalent bonds in membrane proteins is 70-150 kcal, making them strong.

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    Peripheral proteins

    Proteins loosely attached to the membrane via electrostatic and hydrogen bonds, lacking hydrophobic sequences.

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    Lipid-anchored proteins

    Peripheral proteins bound by lipid molecules that are found on one bilayer surface.

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    Protein movement in membranes

    Movement of proteins is lateral and rotational, restricted compared to phospholipids, and can be temperature-dependent.

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    Protein complexes

    Large complexes of membrane proteins that participate in processes like proton pumping and signal transduction.

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    Membrane carbohydrates

    Sugar-containing molecules like glycoproteins and glycolipids that play roles in cell recognition and signaling.

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    Study Notes

    Biological Membrane

    • Composed of cell structures (eukaryotic): cell membrane, nucleus, organelles, cytoplasm, mitochondria, and cytoskeleton.
    • Cell membrane functions: separating the inside from the outside, maintaining concentration gradients, maintaining membrane potential, binding cells together, mediating and regulating cell transport, and detecting and transmitting electrical and chemical signals.
    • Cell membrane theory evolution: Overton (1890s): lipid nature of biological membranes; Langmuir (1917): lipid monolayer (hydrophilic/hydrophobic surfaces); Gorter and Grendel (1925): lipid bilayer; Danielli and Davson (1935): lipid bilayer + protein sheets ("sandwich" model); Singer and Nicolson (1972): fluid-mosaic theory; Unwin and Henderson (1975): membrane proteins contain transmembrane segments; Recent Findings (2000): Lipid rafts.
    • Cell membrane (7-10nm): fluid mosaic theory (Singer and Nicolson, 1972); membrane lipids (40%): bilayer, phospholipids (dominate the structure), cholesterol (provides stability); membrane proteins (52%): peripheral proteins (confined to the surface), integral proteins (span the membrane); and membrane carbohydrates (8%): glycoproteins, glycolipids.

    Membrane Structure

    • Phospholipids: amphipathic molecules (one water-soluble portion and one lipid-soluble portion) with hydrophilic head groups and hydrophobic hydrocarbon tails. Spontaneously aggregate into a bilayer in water, with phosphate head groups facing outward toward the aqueous environment inside and outside the cell.
    • Cholesterol: stabilizes the membrane and changes its fluidity. Lipid rafts are aggregations of lipids and cholesterol.

    Membrane Fluidity

    • Decreased cholesterol decreases fluidity
    • Decreased saturation (FA) increases fluidity
    • Increased cholesterol increases saturation (FA) increases fluidity
    • Decreased temperature decreases fluidity
    • Increased temperature increases fluidity
    • Length of fatty acids (FA) affects fluidity. Short-chain FA increases fluidity, and long-chain FA decreases fluidity.
    • Degree of saturation of FA affects fluidity. Unsaturated FA increases fluidity, and saturated FA decreases fluidity.

    Lipid Composition

    • Phospholipids are the most abundant lipid component in cell membranes.
    • Phosphoglycerides (glycerol-based) and sphingolipids (sphingosine-based) are two major classes.
    • Sterols (e.g., cholesterol in animals, ergosterol in fungi) are present in some membranes and influence fluidity.
    • Lipids are distributed unequally in two monolayers. Glycolipids are mostly on the outer layer.

    Protein Composition

    • Integral proteins: covalently attached to fatty acid chains in the hydrophobic interior and typically have hydrophobic transmembrane segments.
    • Peripheral proteins: attached to exposed polar heads of membrane lipids or integral proteins by weak electrostatic forces and hydrogen bonds.

    Lipid-Anchored Proteins

    • GPI-anchored proteins: covalently attached to glycosylphosphatidylinositol (GPI).

    Protein Movements

    • Protein movement within the membrane may be lateral and rotational (more restricted than phospholipids).

    Membrane Carbohydrates

    • Carbohydrates are attached to proteins (glycoproteins) or lipids (glycolipids) by glycosylation.

    Cell-Cell Adhesion

    • Cells are connected through extracellular matrix (ECM) components (like collagen, elastin, and fibronectin).
    • Specialized cell junctions include tight junctions, desmosomes, and gap junctions.

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    Related Documents

    Biological Membrane PDF

    Description

    Explore the complex structure and functions of biological membranes in eukaryotic cells. This quiz covers essential topics including the evolution of membrane theory, the fluid mosaic model, and key cellular components like the mitochondria and cytoplasm. Test your understanding of how cell membranes regulate transport and signal transduction.

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