BIOL1X07 Revision Quiz 2021
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Questions and Answers

What direction does RNA polymerase synthesize RNA?

  • N to C
  • C to N
  • 5' to 3' (correct)
  • 3' to 5'

    • What is the function of DNA helicase?

  • Unwind part of the DNA (correct)
  • Set a piece for replication to occur in the lagging strand
  • Cut DNA at certain areas
  • Perform the replication of DNA

    • Which one of the following statements is true?

  • mRNA contains a template for protein synthesis (correct)
  • RNA forms B-type structures
  • New strands of DNA are copied 3' to 5'
  • tRNA combines with proteins to form the machinery for protein synthesis (correct)

    • What is the role of DNA topoisomerase in DNA replication?

    <p>Prevents supercoiling of DNA</p> Signup and view all the answers

    Where is the site of transcription?

    <p>Nucleus</p> Signup and view all the answers

    Which of the following are characteristic of a bacterial genome?

    <p>Circular chromosomes</p> Signup and view all the answers

    According to the Central Dogma of molecular biology, which of the following forms of synthesis is NOT possible:

    <p>Protein to RNA</p> Signup and view all the answers

    Which of the following best describes epigenetics?

    <p>Chemical modifications that alter the way a DNA sequence is read</p> Signup and view all the answers

    Which proteins are involved in RNA transcription?

    <p>RNA polymerase</p> Signup and view all the answers

    Which enzyme is responsible for replacing RNA primers with DNA on the lagging strand during DNA replication?

    <p>DNA Polymerase I</p> Signup and view all the answers

    Based on the central dogma of molecular biology, genetic material cannot undergo:

    <p>Reverse translation</p> Signup and view all the answers

    Which one is a nucleobase that can only be found in RNA?

    <p>Uracil</p> Signup and view all the answers

    When comparing the melting temperatures of two pieces of DNA, why would one DNA have a higher melting temperature than the other?

    <p>The DNA has more G/C base pairing that can form 3 hydrogen bonds between nucleobases</p> Signup and view all the answers

    What type of single point mutation would never impact protein structure?

    <p>Silent</p> Signup and view all the answers

    What wavelength do proteins usually absorb most strongly?

    <p>280nm</p> Signup and view all the answers

    During DNA replication, which enzyme is responsible for preventing DNA supercoiling after the double stranded helix is unwound?

    <p>Topoisomerase</p> Signup and view all the answers

    Study Notes

    BIOL1X07 Revision Quiz Questions 2021

    • Course: From Molecules to Ecosystems (University of Sydney)
    • Format: Multiple choice questions from BIOL1X07 students.
    • Use: Revision resource for BIOL1X07 students.

    Module 1: Information Transfer

    • Question 1: What direction does RNA polymerase synthesise RNA?

      • 5' to 3'

    • Question 2: What is the function of DNA helicase?

      • Unwind part of the DNA to allow replication.
      • Sets a piece for replication on the lagging strand.

    • Question 3: Which statements are true?

      • RNA forms B-type structures.
      • mRNA contains the template for protein synthesis.
      • tRNA combines with proteins to form machinery for protein synthesis.

    • Question 4: What is the role of DNA topoisomerase in DNA replication?

      • Prevents supercoiling of DNA.

    • Question 5: Where is the site of transcription?

      • Nucleus

    • Question 6: If 2 genetic bases were used instead of 4 how long would codons need to be to code for all 20 amino acids?

      • 5

    • Question 7: Which characteristics describe a bacterial genome?

      • Large genomes (~10Mb - 150GB).
      • Circular chromosomes.

    • Question 8: According to the Central Dogma of molecular biology, which synthesis is not possible?

      • RNA to DNA

    Module 2: Energy Systems & Cells

    • Question 22: Which statement is true for CAM photosynthesis?

      • Temporal separation occurs; stomata open at night and closed during the day.

    • Question 23: In Krebs cycle, a six carbon compound from acetyl CoA is…

      • Citric acid.

    • Question 24: Where does glycolysis occur?

      • Cytosol

    • Question 25: Which is true regarding photosynthesis?

      • C4 photosynthesis is more water-efficient than CAM photosynthesis.

    • Question 26: Which type of photosynthesis is the most water-efficient?

      • CAM photosynthesis.

    • Question 27: Which of the following does NOT demonstrate plant respiration flexibility?

      • Using CAM photosynthesis in dry habitats to limit water loss.

    • Question 28: What is the primary purpose of Alternative Oxidase and the uncoupling protein?

      • To prevent possible damage to the electron transport chain.

    • Question 29: How many photosystems are involved in non-cyclic phosphorylation?

      • 2

    • Question 30: Why is Rubisco's oxygenase activity problematic?

      • Oxygenation produces a waste product which cannot enter the Calvin cycle and is energetically expensive to deal with.

    • Question 31: What is the difference between C3 and C4 photosynthesis?

      • C3 produce a three carbon compound via Calvin Cycle, while C4 make intermediate four-carbon compound that splits into three carbon compound for Calvin cycle.

    • Question 32: What metabolic pathways do autotrophs and heterotrophs both share?

      • Glycolysis and oxidative phosphorylation

    • Question 33: What is one difference between the light dependent and light independent reactions in photosynthesis?

      • Light dependent produces oxygen, Light independent produces glucose.

    • Question 34: What is the major evolutionary event that maximized light capture by plants?

      • Using sunlight to make both ATP and NADPH.

    Module 3: Global Health

    • Question 74: Why use E.coli as a host instead of yeast?

      • Fastest growth.
      • Easy to extract.
      • Better for expressing eukaryotic genes.
      • Recognized as safe.

    • Question 75: Which statements is correct regarding agar plates?

      • Mannitol Salt Agar is Differential and Enriched.

    • Question 76: Which bacteria can grow well in cold temperatures?

      • Listeria.

    • Question 77: Which group of organisms accounts for the greatest proportion of global diversity? - Bacteria.

    • Question 78: Soil sample colony count calculation.

      • 1.14 x 108 CFU.

    • Question 79: What were the contributions of Howard Florey and Ernst Chain regarding penicillin?

      • Purified penicillin and found it could cure mice of bacterial infection.

    • Question 80: Which of the following is not a microbial heterotroph?

      • Methanotrophs.

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    BIOL1X07 Revision Quiz Questions 2021 PDF

    Description

    Prepare for your BIOL1X07 exam with this revision quiz focused on Information Transfer. This multiple-choice quiz covers essential concepts such as RNA synthesis, DNA replication, and the roles of various enzymes. Ideal for University of Sydney students looking to solidify their understanding.

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