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Questions and Answers

What is the main advantage of using BLOSUM for comparing sequences?

It is better suited for comparing distantly related sequences (correct)

It is faster than other algorithms

It is more sensitive to sequence divergence

It has a higher accuracy rate

Which type of BLAST algorithm is specifically designed for searching short similar sequences?

blastx

tblastn

blastn (correct)

megablast

What feature of a gene does an exon represent?

The part that is protein coding (correct)

The overall length of the gene

The untranslated regulatory region at the gene ends

The initial seed for cross-species comparisons

What does a heuristic algorithm do in the context of solving a problem?

Limits the search space and time without finding the optimal solution Signup and view all the answers

Which BLAST program is best suited for closely related sequences with a target percent identity of 95% or more?

megablast Signup and view all the answers

What is the main difference between blastn and tblastx algorithms?

The direction of sequence translation Signup and view all the answers

What does the term Megablast refer to in the context of the provided text?

Megablast is a program for analyzing short similar sequences. Signup and view all the answers

What is the main difference between BLOSUM and PAM matrices?

BLOSUM matrices are used for closely related sequences, while PAM matrices are for distantly related species. Signup and view all the answers

What is the significance of having a lower N50 value when analyzing sequences?

Lower N50 values imply fewer gaps and higher confidence in the sequence. Signup and view all the answers

What is a Contig in the context of genetic sequencing?

A Contig represents a larger portion of a read with some gaps. Signup and view all the answers

In what way does the Blossom algorithm differ from the Pam matrix?

Blossom focuses on observed substitutions, while Pam focuses on mutation rates. Signup and view all the answers

When choosing between Blastn, Blastp, and Blastx programs, which would be most suitable for short dissimilar sequences?

Blastp Signup and view all the answers