Biofilm Dispersal and Formation

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What type of methods can be used for biofilm clearance?

Only enzymatic degradation
Both mechanical and chemical methods (correct)
Only mechanical methods such as scraping or brushing
Neither mechanical nor chemical methods

What is the primary purpose of covering samples with crystal violet/safranine for 10 minutes?

To remove existing biofilms
To identify the type of microorganisms
To stain the samples for clear observation (correct)
To quantify biofilms

What is the purpose of using a 96-well plate in the experiment?

To grow microorganisms
To store chemicals
To observe biofilm clearance
To establish biofilm formation (correct)

What is the ideal temperature for incubation in the experiment?

37°C (C) Signup and view all the answers

What is the duration for which the plate is left in the incubator?

3 days (A) Signup and view all the answers

What is the role of nutrient broth in the experiment?

To provide nutrients for microorganisms (A) Signup and view all the answers

What is the primary objective of assessing biofilm formation?

To understand the formation, structure, and behavior of biofilms in various environments (C) Signup and view all the answers

Which of the following methods is used for indirect quantification of biofilm formation?

Performing a crystal violet/safranine assay (A) Signup and view all the answers

What is the purpose of incubating the setup at room temperature in static conditions for 5-7 days?

To facilitate the formation of a biofilm (B) Signup and view all the answers

What is the purpose of discarding the planktonic cells after incubation?

To remove non-attached cells from the biofilm (B) Signup and view all the answers

Which of the following materials is not used in the practical session for assessing biofilm formation?

Agar plates (A) Signup and view all the answers

What is the purpose of using an inoculating loop to select single colonies?

To select a single colony from a monoculture (A) Signup and view all the answers

What is the purpose of measuring the optical density at 600 nm?

To determine the concentration of biofilm (A) Signup and view all the answers

What is the purpose of adding crystal violet/safranine to the wells?

To solubilize the biofilm (A) Signup and view all the answers

What is indicated by the presence of a white well?

Effective anti-biofilm agent (A) Signup and view all the answers

What is the purpose of incubating the plate at 37 °C for 24 hours?

To induce the formation of biofilm (A) Signup and view all the answers

What is the purpose of biofilm inhibition?

To prevent the formation of biofilms (D) Signup and view all the answers

What is an example of a mechanical method for preventing biofilm formation?

Scraping the surface (A) Signup and view all the answers

What does a high density of violet color in the wells indicate in the biofilm clearance experiment?

Presence of established biofilm (B) Signup and view all the answers

What can be inferred from a low density of violet color in the wells after treatment with anti-biofilm agents?

The biofilm formation is inhibited (D) Signup and view all the answers

What does it indicate when the optical density (O.D.) after treatment with Ciprofloxacin is similar to the control well?

Ciprofloxacin has no effect on biofilm formation (B) Signup and view all the answers

What is the purpose of adding glacial acetic acid or methanol to the wells?

To solubilize the biofilm (A) Signup and view all the answers

What is the significance of the 600 nm wavelength in the biofilm clearance experiment?

It is the wavelength used to measure the optical density (O.D.) (C) Signup and view all the answers

What is the purpose of the Lab Report in the biofilm clearance experiment?

All of the above (D) Signup and view all the answers

What happens to individual bacteria during the dispersal phase?

They detach from the biofilm and return to a planktonic state (C) Signup and view all the answers

What is the role of extracellular enzymes in biofilm dispersal?

They degrade the EPS and disperse bacterial biofilms (C) Signup and view all the answers