Bioenergetics and ATP Production Quiz
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Questions and Answers

What does the energy difference (ΔG) refer to in bioenergetics?

  • The rate of energy production in a metabolic reaction.
  • The difference between the initial and final energy levels of a reaction. (correct)
  • The amount of energy released by a metabolic process.
  • The efficiency of energy transfer in a biological system.

What is a characteristic of a reaction with a negative ΔG?

  • It is a reversible reaction.
  • It occurs spontaneously. (correct)
  • It requires energy input to proceed.
  • It is an endothermic process.

Which of the following is NOT a way that ATP is produced in biological systems?

  • Deamination (correct)
  • Oxidative phosphorylation
  • Substrate-level phosphorylation
  • Photophosphorylation

What is the primary role of NADH and FADH2 in ATP production?

<p>They carry electrons for oxidative phosphorylation. (A)</p> Signup and view all the answers

What is the correct sequence of events in the process of glycolysis?

<p>Glucose, fructose 6-phosphate, glucose 6-phosphate, pyruvate. (A)</p> Signup and view all the answers

Which of the following statements is TRUE about the pentose phosphate pathway?

<p>It generates NADPH and ribose 5-phosphate. (B)</p> Signup and view all the answers

What is the role of gluconeogenesis in carbohydrate metabolism?

<p>Synthesis of glucose from non-carbohydrate sources. (C)</p> Signup and view all the answers

Which of the following is a key regulatory enzyme in glycolysis?

<p>All of the above (D)</p> Signup and view all the answers

Which of the following is NOT a product of the TCA cycle?

<p>Glucose (B)</p> Signup and view all the answers

What is the role of the electron transport chain in oxidative phosphorylation?

<p>To generate a proton gradient across the mitochondrial membrane. (C)</p> Signup and view all the answers

How does fermentation differ from aerobic respiration?

<p>Fermentation does not use oxygen as an electron acceptor. (D)</p> Signup and view all the answers

What is the main function of the TCA cycle in energy metabolism?

<p>To produce NADH and FADH2 for oxidative phosphorylation. (B)</p> Signup and view all the answers

What is the relationship between glycolysis and the TCA cycle?

<p>Glycolysis produces pyruvate, which is the starting molecule for the TCA cycle. (C)</p> Signup and view all the answers

Which of the following is a key difference between substrate-level phosphorylation and oxidative phosphorylation?

<p>Substrate-level phosphorylation produces ATP directly from a substrate, while oxidative phosphorylation uses an electron transport chain. (B)</p> Signup and view all the answers

Which of the following processes is primarily responsible for maintaining blood glucose levels during periods of fasting?

<p>Gluconeogenesis (D)</p> Signup and view all the answers

What is the main role of glycogen in carbohydrate metabolism?

<p>To provide a readily available source of glucose. (A)</p> Signup and view all the answers

What is the primary function of the pentose phosphate pathway?

<p>Production of NADPH and ribose 5-phosphate. (A)</p> Signup and view all the answers

What type of molecule is glycogen classified as?

<p>Homopolysaccharide (A)</p> Signup and view all the answers

In the structure of glycogen, what type of linkages are found in the branched part?

<p>α-(1,6) (B)</p> Signup and view all the answers

What is the primary function of glycogen stored in the liver?

<p>To regulate blood glucose levels for the entire body (C)</p> Signup and view all the answers

What substance is required to activate glucose 1-phosphate before it can be integrated into glycogen?

<p>UDP (D)</p> Signup and view all the answers

What is the role of glucose 6-phosphate in metabolism?

<p>It can form pyruvate via glycolysis (D)</p> Signup and view all the answers

Which of the following products is generated during the oxidative phase of the pentose phosphate pathway?

<p>Ribose 5-phosphate (B)</p> Signup and view all the answers

Which of the following statements about the non-oxidative reactions of the pentose phosphate pathway is correct?

<p>They generate nucleotide and nucleic acid precursors. (B)</p> Signup and view all the answers

What is the center protein associated with glycogen?

<p>Glycogenin (B)</p> Signup and view all the answers

How many protons does 1 NADH pump across the membrane during electron transport?

<p>3 protons (B)</p> Signup and view all the answers

Which shuttle converts NADH into FADH2 inside the mitochondria?

<p>Glycerol 3-phosphate shuttle (A)</p> Signup and view all the answers

What is the total potential ATP yield from 1 mole of glucose during oxidative metabolism?

<p>36 or 38 ATP (A)</p> Signup and view all the answers

Which of these is NOT a source of blood glucose?

<p>Protein metabolism (D)</p> Signup and view all the answers

During glycolysis, how many ATP molecules are produced from 1 mole of glucose?

<p>2 ATP (A)</p> Signup and view all the answers

Which enzyme is involved in converting pyruvate into Acetyl-CoA?

<p>Pyruvate dehydrogenase (PDH) (B)</p> Signup and view all the answers

What alternative pathway can utilize glucose to produce ribose 5-phosphate?

<p>Pentose phosphate pathway (B)</p> Signup and view all the answers

What is the function of phosphofructokinase-1 (PFK-1) in carbohydrate metabolism?

<p>Catalyzing the first step of glycolysis (C)</p> Signup and view all the answers

Which of the following molecules appears as a result of gluconeogenesis?

<p>Glucose 6-phosphate (A)</p> Signup and view all the answers

Flashcards

What is glycogen?

A branched-chain polysaccharide made of alpha-D-glucose units. The linear part of the chain is linked by alpha-1,4 glycosidic bonds, while branches are formed by alpha-1,6 glycosidic bonds. A central protein called glycogenin serves as a core for glycogen synthesis.

What is the primary function of glycogen in skeletal muscle?

Glycogen stored in skeletal muscle is primarily used to provide energy for muscle contraction. It's a local and fast source of glucose 1-phosphate, which can be quickly converted to glucose for energy production.

What is the primary function of glycogen in the liver?

The liver stores glycogen as a reservoir for the entire body. It's essential for maintaining blood glucose levels, especially during fasting or between meals.

How is glycogen synthesized?

Glycogen synthesis involves converting glucose to glucose 1-phosphate, which is then activated by UDP to form UDP-glucose. UDP-glucose is then added to the growing glycogen chain.

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How is glycogen degraded?

Glycogen degradation breaks down glycogen into glucose 1-phosphate units. This process involves removing glucose units from the end of the chains and from the branching sites.

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What is the main function of the pentose phosphate pathway?

The pentose phosphate pathway (PPP) is a metabolic pathway that produces two important products: ribose 5-phosphate, used in the biosynthesis of nucleotides and nucleic acids, and NADPH, a reducing agent important in anabolic reactions and protection from oxidative stress.

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Describe the oxidative reactions of the pentose phosphate pathway.

The oxidative reactions of the PPP are irreversible and produce NADPH and CO2. They involve the conversion of glucose 6-phosphate to ribulose 5-phosphate.

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Describe the non-oxidative reactions of the pentose phosphate pathway.

The non-oxidative reactions of the PPP are reversible and involve interconversions of sugar phosphates. This allows the pathway to produce intermediates that can be used in other metabolic pathways, such as glycolysis, and to provide the necessary amount of ribose 5-phosphate for nucleotide synthesis.

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Electron Transport Chain (ETC)

A series of protein complexes in the inner mitochondrial membrane that facilitates the transfer of electrons from NADH and FADH2 to molecular oxygen, generating a proton gradient used for ATP synthesis.

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ATP

A molecule that stores and releases energy in cells. It's composed of adenine, ribose, and three phosphate groups.

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Oxidative Phosphorylation

The process of generating ATP from the energy of the proton gradient across the inner mitochondrial membrane, driven by the electron transport chain.

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Pyruvate Dehydrogenase (PDH) Complex

The process of converting pyruvate to acetyl-CoA, connecting glycolysis to the citric acid cycle. It occurs in the mitochondrial matrix.

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Citric Acid Cycle (Krebs Cycle)

A series of reactions that takes place in the mitochondrial matrix, breaking down acetyl-CoA to carbon dioxide, generating ATP, NADH, and FADH2.

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Gluconeogenesis

The process of synthesizing glucose from non-carbohydrate sources like pyruvate, lactate, or amino acids, primarily in the liver.

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Glycogenolysis

The breakdown of glycogen, a stored form of glucose, into glucose molecules. It occurs in the liver and muscle cells.

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Glycogenesis

The process of storing glucose as glycogen, primarily in the liver and muscle cells. It occurs when blood glucose levels are high.

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Pentose Phosphate Pathway

A metabolic pathway that converts glucose into ribose 5-phosphate, a precursor for nucleotide synthesis, and NADPH, a reducing agent used in biosynthesis.

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Glycerol 3-Phosphate Shuttle

A type of shuttle system that transfers reducing equivalents (electrons) from cytoplasmic NADH into the mitochondria, ultimately generating FADH2 within the mitochondria.

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What is bioenergetics?

Bioenergetics is the study of energy transfer and utilization within living organisms. It helps understand whether a process is energetically favorable or not.

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What does ΔG represent in bioenergetics?

The change in free energy (ΔG) represents the energy difference between the initial and final states of a reaction. A negative ΔG indicates an exergonic (spontaneous) reaction, while a positive ΔG indicates an endergonic (non-spontaneous) reaction.

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What is the primary energy currency in cells?

ATP (adenosine triphosphate) is the primary energy currency in cells. It releases energy when a phosphate bond is broken, forming ADP and inorganic phosphate.

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What is substrate-level phosphorylation?

Substrate-level phosphorylation refers to the direct transfer of a phosphate group from a high-energy substrate molecule to ADP to form ATP. This occurs during glycolysis.

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What is oxidative phosphorylation?

Oxidative phosphorylation is the main way ATP is generated in cells. It takes place in the mitochondria and involves electron transport chain and chemiosmotic coupling.

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What is glycolysis?

Glycolysis is the breakdown of glucose into pyruvate in the cytoplasm. It yields a small amount of ATP and NADH.

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What is gluconeogenesis?

Gluconeogenesis is the synthesis of glucose from non-carbohydrate sources, like pyruvate, lactate, or glycerol. This process occurs primarily in the liver and is important for maintaining blood glucose levels.

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What is the pentose phosphate pathway?

The pentose phosphate pathway is an alternative glucose metabolism pathway that produces NADPH and ribose 5-phosphate, crucial for nucleotide biosynthesis and reducing power in the cell.

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What is glycogenolysis?

Glycogenolysis is the breakdown of glycogen, a stored form of glucose, into glucose to supply energy. This process is essential during fasting or exercise.

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What is glycogenesis?

Glycogenesis is the synthesis of glycogen from glucose, primarily in the liver and muscle. This process allows excess glucose to be stored for later use.

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What is the citric acid cycle?

The citric acid cycle, also known as the Krebs cycle or TCA cycle, is a series of biochemical reactions in the mitochondria that oxidizes acetyl-CoA to produce electron carriers (NADH and FADH2) and carbon dioxide. The cycle also provides intermediates for various biosynthetic pathways.

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What is oxidative phosphorylation?

Oxidative phosphorylation is the process by which ATP is produced using the energy released from electron transport chain. It is a major source of ATP in aerobic organisms.

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What is the electron transport chain?

The electron transport chain consists of a series of protein complexes located in the inner mitochondrial membrane. Electrons are passed along the chain, releasing energy used to pump protons across the membrane, ultimately leading to ATP production.

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What are NADH and FADH2?

NADH and FADH2 are electron carriers that transfer electrons from glycolysis and the citric acid cycle to the electron transport chain. They carry high-energy electrons and donate them to the chain, contributing to ATP production.

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What is pyruvate?

Pyruvate is a three-carbon molecule produced during glycolysis. It can be further metabolized by the citric acid cycle to produce energy or used for other biosynthetic pathways.

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What is Acetyl-CoA?

Acetyl-CoA is a two-carbon molecule that enters the citric acid cycle. It is produced from pyruvate and is a crucial link between glycolysis and the citric acid cycle.

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Study Notes

Bioenergetics

  • Bioenergetics is the transfer and utilization of energy in biological systems
  • Bioenergetics predicts if a process is possible, while kinetics measures the reaction rate
  • Enzymes cannot create a reaction by themselves

Free Energy

  • Free energy (G) measures the energy available to do work in a system.
  • A negative change in free energy (ΔG) indicates a spontaneous reaction (exergonic)
  • A positive change in free energy (ΔG) indicates a non-spontaneous reaction (endergonic)
  • Energy difference (ΔG): (Final E) - (Initial E)

ATP Production

  • ATP is the primary energy currency of the cell
  • ATP can undergo hydrolysis (breaking down with water), releasing energy
  • Substrate-level phosphorylation is a metabolic reaction that involves the direct transfer of a phosphate group to ADP from another phosphorylated substrate.
  • Oxidative phosphorylation occurs in the electron transport chain, generating a large number of ATP molecules.

Learning Objectives (Bioenergetics and Carbohydrate Metabolism)

  • Describe bioenergetics
  • Discuss energetics in metabolic reactions
  • List ATP production ways
  • List pathways of glucose inside the cell
  • Describe glycolysis and gluconeogenesis
  • Explain the pentose phosphate pathway
  • Describe glycogenolysis and glycogenesis
  • Explain the citric acid cycle and electron transport chain

Carbohydrate Metabolism

  • Glucose is an important energy source for the body.
  • Glycogen is a storage form of glucose in animals.
  • Glycolysis is the metabolic pathway that converts glucose into pyruvate.
  • Gluconeogenesis is the metabolic pathway that converts non-carbohydrate precursors into glucose.
  • Glycogenolysis is the breakdown of glycogen
  • Glycogenesis is the formation of glycogen
  • Pentose phosphate pathway produces NADPH and Ribose 5-phosphate

Glycolysis

  • Glycolysis is the breakdown of glucose into pyruvate, generating ATP and NADH
  • Glycolysis occurs in the cytoplasm
  • Glycolysis has two stages: investment stage and energy payoff stage.

TCA Cycle (Citric Acid Cycle)

  • The TCA cycle is a central metabolic pathway in aerobic respiration
  • The TCA cycle is a series of oxidation reactions in the mitochondria.
  • Acetyl-CoA enters the TCA cycle and is oxidized.
  • The cycle produces carbon dioxide, ATP, NADH, and FADH2.

Oxidative Phosphorylation and ETC

  • Oxidative phosphorylation is the process that harnesses energy from NADH and FADH2 to create ATP
  • NADH and FADH2 pass electrons to the ETC (electron transport chain).
  • The ETC creates a proton gradient that is used to produce ATP.

Fermentation

  • Fermentation is an anaerobic process that regenerates NAD+ from NADH, with the production of lactate or ethanol
  • Fermentation is used in the absence of oxygen to provide ATP to skeletal muscles.

Pentose Phosphate Pathway

  • Pentose phosphate pathway is an alternative pathway for glucose metabolism
  • NADPH is created from this pathway
  • The pathway gives Ribose 5-phosphate which is used for nucleotide and nucleic acid synthesis.

Hormonal Regulation of Glycolysis

  • Insulin supports glycolysis
  • Glucagon doesn't support glycolysis.

Glycogen

  • Glycogen is a branched polysaccharide storage form of glucose.
  • Glycogen is stored in the liver and skeletal muscles.
  • Glycogen can be broken down to release glucose rapidly.

Transfer of Cytoplasmic NADH to Mitochondria

  • Malate-aspartate shuttle
  • Glycerol 3-phosphate shuttle
  • NADH produced in the cytosol cannot directly enter the mitochondria. These shuttle mechanisms transport the reducing equivalents for ATP production.

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Test your knowledge on bioenergetics, free energy, and ATP production. This quiz covers essential concepts such as reaction spontaneity and the mechanisms of ATP synthesis. Review the key processes and their significance in biological systems.

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