Biochemistry ETC and Hormones Quiz
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Questions and Answers

Which of the following is a 6-carbon molecule?

  • Succinate
  • Citrate (correct)
  • Acetyl CoA
  • Oxaloacetate
  • Which of the following is a metabolic pathway that involves the breakdown of complex molecules into simpler ones, releasing energy?

  • Anabolism
  • Catabolism (correct)
  • Amphibolism
  • Ketogenesis
  • During electron transport, protons are not pumped out of the mitochondrion at which complex?

  • Complex III
  • Complex I
  • Complex IV
  • Complex II (correct)
  • Glycogenolysis can be stimulated non-hormonally by the accumulation of which molecule?

    <p>AMP</p> Signup and view all the answers

    Which metabolic pathway is responsible for the rapid rise in lactate concentration during a 400-m sprint?

    <p>Anaerobic glycolysis</p> Signup and view all the answers

    Which metabolic pathway is responsible for the decline in lactate level after the 400-m sprint?

    <p>Cori cycle</p> Signup and view all the answers

    Which hormone(s) can stimulate glycogenolysis?

    <p>Glucagon and Epinephrine</p> Signup and view all the answers

    Which of the following mammalian cells is unable to aerobically metabolize glucose to carbon dioxide?

    <p>Muscle cells</p> Signup and view all the answers

    What is/are the fate(s) of pyruvate?

    <p>All of the above</p> Signup and view all the answers

    Which of the following is a product of the citric acid cycle?

    <p>All of the above</p> Signup and view all the answers

    Which of the following is not a substrate for gluconeogenesis?

    <p>Fatty acids</p> Signup and view all the answers

    Which of the following is a correct statement regarding the process of lipolysis?

    <p>Lipolysis is the breakdown of triacylglycerol into free fatty acids and glycerol</p> Signup and view all the answers

    In metabolic pathways, which process involves the synthesis of ketone bodies from acetyl CoA?

    <p>Ketogenesis</p> Signup and view all the answers

    The term 'glycogenesis' is correctly defined as:

    <p>The synthesis of glycogen from glucose</p> Signup and view all the answers

    Which of the following correctly describes glycolysis?

    <p>Glycolysis occurs in the presence and absence of oxygen</p> Signup and view all the answers

    In the chemiosmotic hypothesis, which component does NOT play a role?

    <p>The movement of water across cell membranes</p> Signup and view all the answers

    Which scenario would disrupt the chemiosmotic gradient according to the text?

    <p>Increase in ADP and Pi acceptors</p> Signup and view all the answers

    Which method can be used to identify bacteria based on enzyme activity and substrate utilization?

    <p>Metabolic / biochemical</p> Signup and view all the answers

    What is the primary purpose of FISH in bacterial classification?

    <p>Identifying genetic sequences</p> Signup and view all the answers

    Which bacterial genus is associated with tetanus and botulism?

    <p>Clostridia</p> Signup and view all the answers

    Which bacterial species is known for causing pneumonia and is Gram-positive?

    <p>Streptococcus pneumoniae</p> Signup and view all the answers

    Which technique can be utilized to differentiate between different strains of bacteria by analyzing their protein profiles?

    <p>Mass Spectrometry</p> Signup and view all the answers

    Which bacterial species is commonly associated with foodborne illnesses like gastroenteritis?

    <p>Escherichia coli</p> Signup and view all the answers

    What is the significance of core microbiota, as mentioned in the text?

    <p>They carry out essential homeostatic functions</p> Signup and view all the answers

    How does the microbiota composition of monozygotic twins compare to dizygotic twins, based on the text?

    <p>Dizygotic twins have similar variability in microbiota</p> Signup and view all the answers

    What is suggested by the finding that the microbiota of family members is more similar than unrelated individuals?

    <p>Environmental factors play a role in shaping microbiota</p> Signup and view all the answers

    Why is it mentioned that approximately 75 species are common to over 50% of individuals?

    <p>To indicate the presence of core microbiota functions</p> Signup and view all the answers

    What does the text imply about the relationship between microbial ecosystems and human biology?

    <p>Microbial ecosystems and human biology co-evolve together</p> Signup and view all the answers

    In what way do monozygotic twins provide insights into the interplay between genetics and environmental factors in shaping microbiota composition?

    <p>Their microbiota variability resembles that of dizygotic twins</p> Signup and view all the answers

    According to the phylogenetic tree of life shown, which of the following groups is most closely related to animals?

    <p>Fungi</p> Signup and view all the answers

    Based on the image depicting the composition of the human microbiome, which of the following body sites has the most diverse microbial community?

    <p>Gut</p> Signup and view all the answers

    According to the image depicting the evolutionary relationships of wild hominids, which of the following species is most closely related to humans (Homo sapiens)?

    <p><em>Pan troglodytes</em> (chimpanzee)</p> Signup and view all the answers

    Which of the following statements best describes the concept of co-evolution between hosts and their microbial communities?

    <p>Host and microbial genomes influence each other's evolution over time.</p> Signup and view all the answers

    Based on the phylogenetic tree of life shown, which of the following groups is most closely related to bacteria?

    <p>Archaea</p> Signup and view all the answers

    According to the image depicting the composition of the human microbiome, which of the following body sites has the least diverse microbial community?

    <p>Vagina</p> Signup and view all the answers

    Study Notes

    ATP Synthesis and Electron Transport

    • Proton flow into mitochondria depends on the presence of ADP and Pi
    • ATPase activity is reversible
    • Only proton transport is strictly regulated, while other positively charged ions can diffuse freely across the mitochondrial membrane

    Citric Acid Cycle

    • The citric acid cycle produces 6 NADH, 2 FADH2, and 2 ATP

    Glycogenolysis

    • Glycogenolysis can be stimulated by the hormones Glucagon and Epinephrine
    • Non-hormonally, the accumulation of AMP, or low energy level, which indicates low levels of ATP, can stimulate glycogenolysis
    • Glycogenolysis is regulated by:
      • Hormonal inhibition of glycogen synthase by Glucagon and Epinephrine (in muscle)
      • Non-hormonal stimulation by low energy levels (accumulation of AMP)

    Fates of Pyruvate

    • Pyruvate can be involved in:
      • Catabolism
      • Anabolism
      • Amphibolism
      • Ketogenesis
      • Degradation

    Lactate and Anaerobic Glycolysis

    • Anaerobic glycolysis converts pyruvate to lactate
    • During a 400-m sprint, the rapid rise in lactate concentration is caused by anaerobic glycolysis
    • After the run, the decline in lactate level is caused by the Cori cycle and gluconeogenesis
    • The Cori cycle: lactate is converted back to pyruvate, and gluconeogenesis utilizes pyruvate to produce glucose and replace glycogen

    Mammalian Cells and Glucose Metabolism

    • Some mammalian cells are unable to aerobically metabolize glucose to carbon dioxide, such as:
      • RBC (red blood cells)
      • WBC (white blood cells)
      • Reticulocytes

    Gluconeogenesis

    • Gluconeogenesis is the production of glucose from non-carbohydrate molecules
    • Substrates for gluconeogenesis include:
      • Lactate
      • Alanine
      • Glycerol
      • Propionyl CoA
    • Acetyl CoA is not a substrate for gluconeogenesis

    Electron Transport Chain

    • The ETC requires:
      • ADP and Pi as acceptors
      • O2 as the terminal electron acceptor
      • NAD and FAD as electron carriers
    • Lack of ADP and Pi, O2, or NAD and FAD will stop the ETC
    • Uncoupling of the ETC will disrupt the chemiosmotic gradient

    Hormones and Blood Glucose

    • Insulin decreases blood glucose and increases the uptake of glucose in various tissues, such as:
      • Skeletal muscle
      • Adipose tissue

    Metabolic Pathways

    • Gluconeogenesis: the synthesis of glucose from lactate, glycerol, or amino acids
    • Glycogenolysis: the breakdown of glycogen to glucose
    • Lipolysis: the breakdown of triacylglycerol into free fatty acids and glycerol
    • Ketogenesis: the synthesis of ketone bodies from acetyl CoA

    Bacterial Morphology and Classification

    • Bacterial classification involves:
      • Morphological staining (shape, cell wall composition)
      • Metabolic/biochemical (enzyme activity, substrate utilization)
      • Genetic (DNA/RNA, PCR, sequencing, FISH)
      • Immunologic (ELISA, agglutination test)
      • Mass spectrometry (MALDI-TOF)

    Microbiome and Host Interactions

    • The human microbiome is composed of:
      • 25 phyla
      • 2,000 genera
      • 5,000 species
      • 316 M genes
    • Each person has approximately 500-1000 gut species
    • The core microbiota is composed of approximately 75 species common to >50% of individuals
    • Co-maturation of the microbiota and immune system occurs during initial microbial colonization
    • Co-evolution of humans and their microbiota has led to the development of specific microbial communities at different body sites

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    Description

    Test your knowledge on Electron Transport Chain (ETC) and hormones related to blood glucose regulation. Identify true statements about ETC and determine the hormone that decreases blood glucose levels and increases glucose uptake in tissues.

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