Biochemistry Enzymes, Lipids, and Nucleic Acids

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Questions and Answers

What is the primary function of an enzyme?

  • To decrease activation energy (correct)
  • To increase activation energy
  • To act as a substrate
  • To supply energy to the reaction

Which part of the enzyme binds to the substrate?

  • Inhibitor site
  • Receptor site
  • Allosteric site
  • Active site (correct)

Enzymes are composed mainly of which type of macromolecule?

  • Lipids
  • Proteins (correct)
  • Nucleic acids
  • Carbohydrates

The specificity of an enzyme is due to its: a) Shape

<p>Shape (B)</p> Signup and view all the answers

Which of the following is a cofactor that assists enzyme function?

<p>Magnesium ion (D)</p> Signup and view all the answers

Which type of enzyme catalyzes the removal of electrons or hydrogen atoms?

<p>Oxidoreductase (D)</p> Signup and view all the answers

Competitive inhibitors bind to the enzyme at the: a) Allosteric site

<p>Active site (B)</p> Signup and view all the answers

Which of the following factors does NOT directly affect enzyme activity?

<p>Size of the enzyme (A)</p> Signup and view all the answers

Which of the following describes an enzyme's saturation point?

<p>When all enzyme active sites are occupied (D)</p> Signup and view all the answers

What is the effect of increasing substrate concentration on enzyme activity when the enzyme is saturated?

<p>No effect (B)</p> Signup and view all the answers

Lipids are primarily composed of which elements?

<p>Carbon, hydrogen, and oxygen (D)</p> Signup and view all the answers

Which of the following is a function of lipids in the body?

<p>Energy storage (B)</p> Signup and view all the answers

Which of the following is a characteristic of unsaturated fats?

<p>They contain one or more double bonds between carbon atoms (D)</p> Signup and view all the answers

Which lipid is a major component of cell membranes?

<p>Phospho lipids (D)</p> Signup and view all the answers

Which of the following lipids is involved in signaling within the body?

<p>Steroids (D)</p> Signup and view all the answers

A triglyceride consists of: a) Three fatty acids and a glycerol

<p>Three fatty acids and a glycerol (C)</p> Signup and view all the answers

The hydrophobic nature of lipids is due to: a) The presence of polar groups

<p>The large number of C-H bonds (B)</p> Signup and view all the answers

Which of the following is a saturated fat?

<p>Butter (D)</p> Signup and view all the answers

Lipids can be classified into three main groups: triglycerides, phospholipids, and: a) Glycolipids

<p>Steroids (A)</p> Signup and view all the answers

Which of the following statements about cholesterol is true?

<p>Cholesterol is essential for cell membrane structure (B)</p> Signup and view all the answers

Nucleic acids are polymers made up of: a) Amino acids

<p>Nucleotides (A)</p> Signup and view all the answers

The backbone of a DNA molecule consists of: a) Amino acids

<p>Sugar and phosphate groups (A)</p> Signup and view all the answers

Which nitrogenous base is found in RNA but not in DNA?

<p>Uracil (D)</p> Signup and view all the answers

The bond between nucleotides in a nucleic acid is called: a) Glycosidic bond

<p>Phosphodiester bond (B)</p> Signup and view all the answers

Which nucleic acid is responsible for carrying genetic information?

<p>DNA (A)</p> Signup and view all the answers

The process of copying DNA into RNA is called: a) Replication

<p>Transcription (A)</p> Signup and view all the answers

What is the role of tRNA in protein synthesis?

<p>To bind to amino acids and transfer them to the ribosome (D)</p> Signup and view all the answers

Which of the following is a pyrimidine found in DNA?

<p>Cytosine (D)</p> Signup and view all the answers

Which enzyme is responsible for adding nucleotides to a growing DNA strand during replication?

<p>DNA polymerase (A)</p> Signup and view all the answers

Which type of RNA carries the genetic code from DNA to the ribosome?

<p>mRNA (C)</p> Signup and view all the answers

The two strands of DNA are held together by: a) Phosphodiester bonds

<p>Hydrogen bonds (D)</p> Signup and view all the answers

In RNA, adenine pairs with: a) Thymine

<p>Uracil (B)</p> Signup and view all the answers

The sugar found in DNA is: a) Ribose

<p>Deoxyribose (C)</p> Signup and view all the answers

Which of the following nucleotides is NOT found in RNA?

<p>Thymine (D)</p> Signup and view all the answers

The function of rRNA is to: a) Carry amino acids to the ribosome

<p>Form part of the structure of ribosomes (B)</p> Signup and view all the answers

The double helix model of DNA was proposed by: a) Darwin and Mendel

<p>Watson and Crick (B)</p> Signup and view all the answers

During DNA replication, the leading strand is synthesized: a) Discontinuously

<p>In the 5' to 3' direction continuously (A)</p> Signup and view all the answers

Which of the following is a nucleic acid monomer?

<p>Nucleotide (A)</p> Signup and view all the answers

Which type of bond forms between the base pairs in DNA?

<p>Hydrogen bonds (C)</p> Signup and view all the answers

Which of the following best describes the shape of a DNA molecule?

<p>Double helix (D)</p> Signup and view all the answers

Enzymes that remove phosphate groups are known as: a) Kinases

<p>Phosphatases (A)</p> Signup and view all the answers

Which type of lipid forms the bilayer in cell membranes?

<p>Phospholipids (D)</p> Signup and view all the answers

What is the correct pairing of nucleotides in DNA?

<p>A-T, G-C (B)</p> Signup and view all the answers

Which factor does NOT affect the rate of enzyme activity?

<p>Light intensity (D)</p> Signup and view all the answers

In RNA, which nucleotide replaces thymine?

<p>Uracil (D)</p> Signup and view all the answers

Which component of nucleic acids contains nitrogen?

<p>Nitrogenous base (D)</p> Signup and view all the answers

Which lipid is essential for hormone synthesis?

<p>Cholesterol (A)</p> Signup and view all the answers

Enzymes that digest lipids are called: a) Lipases

<p>Lipases (C)</p> Signup and view all the answers

In which part of the cell does DNA replication occur?

<p>Nucleus (A)</p> Signup and view all the answers

Which enzyme is responsible for unwinding the DNA helix during replication?

<p>Helicase (C)</p> Signup and view all the answers

Flashcards

What are enzymes?

Enzymes are biological catalysts that speed up chemical reactions in living organisms without being consumed in the process.

How do enzymes affect activation energy?

Enzymes lower the activation energy needed for a reaction to occur, thus accelerating the reaction rate.

Where does the substrate bind on an enzyme?

The active site is a specific region on an enzyme's surface where the substrate binds.

What are enzymes made of primarily?

Enzymes are primarily composed of proteins, though some may require additional components.

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What determines an enzyme's specificity?

The shape of an enzyme's active site determines its specificity for a particular substrate.

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What are cofactors in relation to enzymes?

Cofactors are non-protein molecules that assist enzymes in their catalytic function.

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What is an oxidoreductase?

Oxidoreductases catalyze oxidation-reduction reactions, involving the transfer of electrons or hydrogen atoms.

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Where do competitive inhibitors bind?

Competitive inhibitors bind to the active site of an enzyme, blocking the substrate from binding.

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What factors affect enzyme activity?

Factors like temperature, pH, and substrate concentration influence enzyme activity.

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What is the saturation point for an enzyme?

Enzyme saturation occurs when all active sites are occupied by substrate molecules, preventing further increases in activity.

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What are lipids?

Lipids are a diverse group of organic compounds characterized by their insolubility in water, but solubility in organic solvents.

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What are some functions of lipids?

Lipids play crucial roles in energy storage, cell membrane structure, hormone production, and insulation.

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What are unsaturated fats?

Unsaturated fats contain one or more double bonds between carbon atoms in their fatty acid chains.

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What are phospholipids?

Phospholipids are a type of lipid that forms the bilayer structure of cell membranes.

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What are steroids?

Steroids are lipid-based molecules involved in signaling pathways, regulating many body functions.

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What makes up a triglyceride?

A triglyceride consists of three fatty acids bonded to a glycerol molecule.

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Why are lipids hydrophobic?

The hydrophobic nature of lipids is due to their long carbon chains with primarily nonpolar C-H bonds.

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What are saturated fats?

Saturated fats have only single bonds between carbon atoms in their fatty acid chains.

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What are the main groups of lipids?

Steroids are a major class of lipids, along with triglycerides and phospholipids.

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What is cholesterol?

Cholesterol is an essential lipid for cell membrane structure and hormone synthesis.

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What are nucleic acids?

Nucleic acids are polymers composed of nucleotide monomers.

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What forms the backbone of DNA?

The backbone of a DNA molecule consists of alternating sugar (deoxyribose) and phosphate groups.

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Which base is unique to RNA?

Uracil is a nitrogenous base found in RNA but not in DNA.

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What is the bond between nucleotides?

The bond linking nucleotides in nucleic acids is called a phosphodiester bond.

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What is the role of DNA?

DNA carries the genetic information that directs the synthesis of proteins.

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What is transcription?

Transcription is the process of copying genetic information from DNA into RNA.

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What is the role of tRNA?

tRNA molecules transport specific amino acids to the ribosome during protein synthesis.

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Which base is a pyrimidine?

Cytosine is a pyrimidine base found in both DNA and RNA.

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What enzyme is responsible for DNA synthesis?

DNA polymerase is the enzyme responsible for adding nucleotides to a growing DNA strand during replication.

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What is the role of mRNA?

mRNA carries the genetic code from DNA to the ribosome, where protein synthesis occurs.

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What holds the DNA strands together?

Hydrogen bonds form between complementary base pairs in DNA, holding the two strands together.

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What are the base pairings in RNA?

In RNA, adenine pairs with uracil, while guanine pairs with cytosine.

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What is the sugar in DNA?

Deoxyribose is the sugar found in DNA.

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Which base is unique to DNA?

Thymine is a pyrimidine base found only in DNA.

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What is the role of rRNA?

rRNA is a component of ribosomes, essential for protein synthesis.

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Who proposed the double helix model of DNA?

Watson and Crick proposed the double helix model of DNA based on experimental data.

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How is the leading strand synthesized?

The leading strand of DNA is synthesized continuously in the 5' to 3' direction during replication.

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What is a nucleotide?

A nucleotide is a monomer for nucleic acids, consisting of a sugar, a phosphate group, and a nitrogenous base.

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What type of bonds hold base pairs together?

Hydrogen bonds form between complementary base pairs in DNA, stabilizing the double helix structure.

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What is the shape of a DNA molecule?

The DNA molecule has a double helix shape, resembling a twisted ladder.

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What are phosphatases?

Phosphatases are enzymes that remove phosphate groups from molecules.

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What is the key function of phospholipids?

Phospholipids form the bilayer structure of cell membranes, creating a barrier between the cell and its environment.

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What are the base pairings in DNA?

In DNA, adenine (A) pairs with thymine (T), and guanine (G) pairs with cytosine (C).

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Does light intensity affect enzyme activity?

Light intensity does not directly influence enzyme activity.

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Which base replaces thymine in RNA?

Uracil replaces thymine in RNA.

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Which component of a nucleotide contains nitrogen?

The nitrogenous base in nucleotides contains nitrogen.

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What is a key function of cholesterol?

Cholesterol is a key lipid for hormone synthesis, including steroid hormones like testosterone and estrogen.

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What do lipases do?

Lipases are enzymes that digest lipids.

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Where does DNA replication take place?

DNA replication occurs in the nucleus of eukaryotic cells.

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What enzyme unwinds the DNA helix?

Helicase is the enzyme responsible for unwinding the DNA helix during replication, separating the two strands.

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Study Notes

Enzymes

  • Enzymes lower activation energy for reactions.
  • Enzymes act as catalysts.
  • Enzymes bind to substrates at the active site.
  • Enzyme specificity is determined by shape.
  • Cofactors, like magnesium ions, assist enzyme function.
  • Oxidoreductases remove electrons or hydrogen atoms.
  • Competitive inhibitors bind to the active site.
  • Enzyme activity is not directly affected by enzyme size.
  • Enzyme saturation occurs when all active sites are occupied, further substrate increase does not increase enzyme activity.
  • Substrate concentration affects enzyme activity until saturation.

Lipids

  • Lipids primarily consist of carbon, hydrogen, and oxygen.
  • Lipids function include energy storage.
  • Unsaturated fats have double bonds between carbons.
  • Phospholipids are a major component of cell membranes.
  • Steroids are involved in signaling within the body.
  • Triglycerides consist of three fatty acids and a glycerol.

Nucleic Acids

  • Nucleic acids are polymers made of nucleotides.
  • DNA's backbone consists of sugar and phosphate groups.
  • DNA contains the nitrogenous bases adenine, thymine, guanine, and cytosine.
  • RNA contains the nitrogenous bases adenine, uracil, guanine, and cytosine.
  • DNA replication occurs in the nucleus.
  • Helicase unwinds DNA during replication.
  • Phosphodiester bonds hold nucleotides together.
  • Hydrogen bonds hold base pairs together.
  • DNA carries genetic information.
  • Transcription copies DNA into RNA.
  • tRNA carries amino acids to ribosomes during protein synthesis.
  • mRNA carries the genetic code to the ribosome.
  • Enzyme phosphatases remove phosphate groups.
  • Lipids like phospholipids make cell membranes.
  • Cholesterol is crucial for hormone synthesis .
  • Lipases digest lipids.

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