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Questions and Answers
What process do fatty acids undergo to be transformed into acetyl CoA for entry into the Kreb's cycle?
What process do fatty acids undergo to be transformed into acetyl CoA for entry into the Kreb's cycle?
- Beta oxidation (correct)
- Lipogenesis
- Glycolysis
- Fermentation
What is the primary role of catabolic processes within a cell?
What is the primary role of catabolic processes within a cell?
- To synthesize lipids
- To provide energy for building reactions (correct)
- To transport nutrients
- To build complex molecules
Which type of microorganism must obtain its carbon in an organic form?
Which type of microorganism must obtain its carbon in an organic form?
- Heterotroph (correct)
- Phototroph
- Autotroph
- Chemotroph
Which of the following nutrients is essential for stabilizing cell walls and endospores in bacteria?
Which of the following nutrients is essential for stabilizing cell walls and endospores in bacteria?
Which nutrient serves as a crucial component of chlorophyll in organisms?
Which nutrient serves as a crucial component of chlorophyll in organisms?
What is the outcome for a cell placed in a hypotonic solution?
What is the outcome for a cell placed in a hypotonic solution?
Which type of microbe gets its energy from chemical reactions?
Which type of microbe gets its energy from chemical reactions?
What type of nutrients do macronutrients represent?
What type of nutrients do macronutrients represent?
What is the primary goal of sterilization in microbial control?
What is the primary goal of sterilization in microbial control?
Which method can distinguish between live and dead cells?
Which method can distinguish between live and dead cells?
What does microbial stasis refer to?
What does microbial stasis refer to?
Which antimicrobial agent is commonly used to destroy vegetative pathogens?
Which antimicrobial agent is commonly used to destroy vegetative pathogens?
What is the effect of surfactants in microbial control?
What is the effect of surfactants in microbial control?
What is the primary function of enzymes in metabolism?
What is the primary function of enzymes in metabolism?
How does heat function as an antimicrobial agent?
How does heat function as an antimicrobial agent?
Which statement about microbial death is accurate?
Which statement about microbial death is accurate?
Which of the following best describes catabolism?
Which of the following best describes catabolism?
What happens during enzyme denaturation?
What happens during enzyme denaturation?
Microbes can be categorized based on resistance. Which group has the highest resistance?
Microbes can be categorized based on resistance. Which group has the highest resistance?
Which group of microbes is characterized by an optimum temperature below 15°C?
Which group of microbes is characterized by an optimum temperature below 15°C?
Which of the following is true about constitutive enzymes?
Which of the following is true about constitutive enzymes?
What type of respiration utilizes $CO3^{2-}$ as a final electron acceptor?
What type of respiration utilizes $CO3^{2-}$ as a final electron acceptor?
What is the primary characteristic of facultative anaerobes?
What is the primary characteristic of facultative anaerobes?
What is the initial process all three metabolic strategies begin with?
What is the initial process all three metabolic strategies begin with?
What defines the optimum temperature for a microorganism?
What defines the optimum temperature for a microorganism?
During which phase of the growth curve do newly inoculated cells require adjustment before division?
During which phase of the growth curve do newly inoculated cells require adjustment before division?
How does the regulation of enzyme synthesis occur?
How does the regulation of enzyme synthesis occur?
What is primarily stored as energy in cells?
What is primarily stored as energy in cells?
What is a common characteristic of halophiles?
What is a common characteristic of halophiles?
What is the purpose of binary fission in microbial growth?
What is the purpose of binary fission in microbial growth?
Which type of microbe is harmed by normal atmospheric concentrations of oxygen but requires a small amount for growth?
Which type of microbe is harmed by normal atmospheric concentrations of oxygen but requires a small amount for growth?
What does the term generation time refer to in microbiology?
What does the term generation time refer to in microbiology?
What is the function of the reverse transcriptase enzyme in HIV?
What is the function of the reverse transcriptase enzyme in HIV?
What is superinfection?
What is superinfection?
Which test provides data on antimicrobial susceptibility?
Which test provides data on antimicrobial susceptibility?
Which of the following best describes multi-drug resistant (MDR) pumps?
Which of the following best describes multi-drug resistant (MDR) pumps?
What does the minimum inhibitory concentration (MIC) represent?
What does the minimum inhibitory concentration (MIC) represent?
What can cause antimicrobial treatment to fail?
What can cause antimicrobial treatment to fail?
What is a significant side effect of drug therapy?
What is a significant side effect of drug therapy?
What role does interferon play in the immune response?
What role does interferon play in the immune response?
What is the thermal death point defined as?
What is the thermal death point defined as?
What is the main goal of pasteurization?
What is the main goal of pasteurization?
Which type of radiation is specifically termed ionizing radiation?
Which type of radiation is specifically termed ionizing radiation?
What is a characteristic of quaternary ammonium compounds?
What is a characteristic of quaternary ammonium compounds?
What action do intermediate-level germicides primarily target?
What action do intermediate-level germicides primarily target?
What is the mechanism by which alcohol inactivates enveloped viruses?
What is the mechanism by which alcohol inactivates enveloped viruses?
What is the primary active component in hydrogen peroxide that contributes to its germicidal effects?
What is the primary active component in hydrogen peroxide that contributes to its germicidal effects?
What characteristic is associated with ethylene oxide (ETO)?
What characteristic is associated with ethylene oxide (ETO)?
Flashcards
Metabolism
Metabolism
The sum of all chemical reactions in a living organism that are necessary to maintain life.
Anabolism
Anabolism
The process of building up complex molecules from simpler ones, requiring energy.
Catabolism
Catabolism
The process of breaking down complex molecules into simpler ones, releasing energy.
Enzymes
Enzymes
Biological catalysts that speed up chemical reactions without being consumed in the process.
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Cofactors
Cofactors
Non-protein molecules that assist enzymes in their function.
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Coenzymes
Coenzymes
Organic cofactors that work in conjunction with enzymes, often derived from vitamins.
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Denaturation
Denaturation
The process where an enzyme loses its shape and function due to changes in its environment.
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Constitutive Enzymes
Constitutive Enzymes
Enzymes present in a cell in constant amounts, their levels don't change with substrate availability.
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Beta Oxidation
Beta Oxidation
The process of breaking down fatty acids into acetyl CoA molecules, which can then enter the Krebs cycle for energy production.
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Deamination
Deamination
The removal of an amino group from an amino acid molecule, typically converting it to ammonia and a keto acid.
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Bioelements
Bioelements
Essential elements that all living organisms require for survival and growth.
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Essential Nutrient
Essential Nutrient
A substance an organism cannot produce on its own and must obtain from its environment.
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Macronutrients
Macronutrients
Nutrients required in large quantities, essential for cell structure and metabolism.
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Micronutrients
Micronutrients
Nutrients required in small quantities, involved in enzyme function and maintaining protein structure.
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Heterotroph
Heterotroph
An organism that obtains its carbon from organic sources, like consuming other organisms.
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Optimum Temperature
Optimum Temperature
The temperature at which a microbe grows and metabolizes at the fastest rate.
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Psychrophiles
Psychrophiles
Microbes that thrive in cold environments, with an optimal temperature below 15°C.
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Mesophiles
Mesophiles
Microbes that prefer moderate temperatures, with an optimal temperature between 20°C and 40°C.
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Obligate Aerobe
Obligate Aerobe
A microbe that absolutely requires oxygen for growth and metabolism.
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Facultative Anaerobe
Facultative Anaerobe
A microbe that can grow in the presence or absence of oxygen, but prefers oxygen for metabolism.
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Binary Fission
Binary Fission
The process of asexual reproduction where one bacterial cell divides into two identical daughter cells.
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Generation Time
Generation Time
The time it takes for a bacterial population to double in size.
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Lag Phase
Lag Phase
The initial phase of bacterial growth in a new environment, where cells adjust and prepare for rapid growth.
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Stationary Phase
Stationary Phase
A stage in microbial growth where the number of new cells produced equals the number of cells dying, resulting in a stable population size.
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Death Phase
Death Phase
The final stage of microbial growth where the number of dying cells exceeds the number of new cells, leading to a decline in population.
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Direct Cell Count
Direct Cell Count
A method of counting microbes directly under a microscope, using a special counting chamber.
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Coulter Counter
Coulter Counter
An electronic device that counts microbes by detecting changes in electrical resistance as cells pass through a tiny opening.
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Flow Cytometer
Flow Cytometer
A sophisticated instrument like a Coulter counter, but it can differentiate between live and dead cells and measure them.
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Sterilization
Sterilization
The process of completely eliminating all viable microorganisms, including viruses and bacterial spores.
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Microbistasis
Microbistasis
A state where microbial growth is temporarily halted but the microbes are not killed.
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Disinfection
Disinfection
The process of killing or inactivating most disease-causing microorganisms, but not bacterial endospores.
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Thermal Death Time
Thermal Death Time
The shortest time required to kill all microbes in a sample at a specific temperature.
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Thermal Death Point
Thermal Death Point
The lowest temperature required to kill all microbes in a sample within 10 minutes.
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Pasteurization
Pasteurization
A heat treatment that kills pathogens and spoilage microbes in liquids without significantly altering their flavor or nutritional content.
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Irradiation
Irradiation
Using radiant energy to disinfect or sterilize materials.
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Ionizing Radiation
Ionizing Radiation
Radiation that ejects electrons from atoms, creating ions.
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Nonionizing Radiation
Nonionizing Radiation
Radiation that excites atoms without ionizing them, like UV light.
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Filtration
Filtration
A method of removing microbes from air or liquids by passing them through a fine filter.
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Alcohol's Action on Viruses
Alcohol's Action on Viruses
Alcohol inactivates enveloped viruses more effectively due to its surfactant effect on the envelope.
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Reverse Transcriptase
Reverse Transcriptase
An enzyme used by retroviruses, like HIV, to convert their RNA genome into DNA, allowing them to integrate into the host cell's genome.
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Interferon
Interferon
A glycoprotein produced by cells in response to viral infection or other immune stimuli, helping to defend against pathogens.
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Drug Resistance
Drug Resistance
The ability of microorganisms to tolerate previously effective antimicrobial drugs, often due to mutations or acquisition of resistance genes.
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MDR Pumps
MDR Pumps
Proteins found in some bacteria that actively pump out antibiotics and other harmful substances, contributing to drug resistance.
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Biofilms
Biofilms
Complex communities of microorganisms embedded in a protective matrix, often found on surfaces and contributing to antibiotic resistance.
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Superinfection
Superinfection
A new infection that emerges during antimicrobial therapy, often caused by drug-resistant microbes overgrowing after the original infection is suppressed.
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Kirby-Bauer Technique
Kirby-Bauer Technique
An agar diffusion test used to assess the susceptibility of bacteria to antimicrobial drugs, by measuring the zone of inhibition around antibiotic discs.
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Minimum Inhibitory Concentration (MIC)
Minimum Inhibitory Concentration (MIC)
The lowest concentration of an antimicrobial drug that inhibits the visible growth of a microorganism.
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Chapter 8 Notes
- Metabolism is the sum of all chemical reactions in the body needed to maintain homeostasis. It includes two types:
- Anabolism: building up macromolecules for structure and function
- Catabolism: breaking down macromolecules to produce energy
- Enzymes are biological catalysts that speed up chemical reactions by lowering activation energy. They are not part of the products and remain unchanged during the process. Cofactors, like coenzymes, support enzyme function.
- Denaturation occurs when weak bonds maintaining an enzyme's shape are broken. This alters the enzyme's shape, preventing substrate attachment to the active site.
- Constitutive enzymes are present in consistent amounts, regardless of substrate levels. Regulated enzymes adjust in concentration based on substrate levels.
- Enzyme activity can be controlled through induction or repression of synthesis.
- Energy is primarily stored as ATP. Oxidation-reduction (redox) reactions transfer energy.
Chapter 7 Notes
- Bioelements are essential elements for all living organisms. Macronutrients are needed in large quantities for structural roles. Micronutrients (trace elements) are involved in maintaining enzyme function.
- Inorganic nutrients include atoms/molecules other than carbon and hydrogen. Organic nutrients contain both carbon and hydrogen. Examples of essential elements include potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn).
- Temperature affects microbial growth. Minimum temperature is the lowest for growth, maximum for highest growth, and optimum for fastest rates. Psychrophiles thrive in cold temperatures, psychotrophs can grow in refrigeration, and thermophiles thrive in high temperatures.
- Carbon and energy sources classify microbes. Heterotrophs use organic carbon, and autotrophs use inorganic carbon. Phototrophs obtain energy from light, and chemotrophs from chemical reactions.
- Microbial growth patterns include lag phase (initial adjustment), exponential growth (log phase/rapid growth), stationary phase (growth rate = death rate), and death phase (significant cell death).
- Different microbes thrive in various environments, with some needing oxygen (aerobes) and others not (anaerobes) and even requiring specific oxygen levels (microaerophiles).
- Microbes can grow in acidic (acidophiles) or basic (alkaliphiles) environments. Different organisms have optimal temperature ranges.
Chapter 11 Notes
- Contaminants are unwanted microbes.
- Decontamination employs physical and chemical agents targeting various groups of microorganisms, with varying degrees of resistance to these agents (highest, moderate, and least).
- Sterilization eliminates all types of microbes, including spores. Disinfection reduces the number of undesirable microbes on non-living surfaces.
- Methods include heat (moist and dry), radiation (ionizing and non-ionizing), and filtration. Chemical agents like alcohols (disinfectant/antiseptic), halogens (chlorine and iodine), hydrogen peroxide, and quaternary ammonium compounds are also used.
Chapter 12 Notes
- Antimicrobial treatment aims to eliminate pathogens without harming the host.
- Antibiotics are produced by microbes that kill or inhibit other microbes.
- Many mechanisms of action exist, targeting cell walls (e.g., penicillin), nucleic acids (e.g., rifampin, sulfonamides), , protein synthesis (e.g., tetracyclines), and diverse metabolic pathways.
- Drug resistance poses challenges. Resistance can occur through various mechanisms in many species of microbes.
- Other outcomes of antimicrobial therapy include treatment failure due to drug inability to penetrate a body compartment or the presence of drug-resistant strains.
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