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Questions and Answers
What is the fundamental principle behind energy release when relocating electrons from sugars to oxygen?
What is the fundamental principle behind energy release when relocating electrons from sugars to oxygen?
Which of the following is NOT a primary function of NADH in cellular metabolism?
Which of the following is NOT a primary function of NADH in cellular metabolism?
What role does electronegativity play in redox reactions?
What role does electronegativity play in redox reactions?
Why are intact membranes essential for chemiosmosis?
Why are intact membranes essential for chemiosmosis?
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In the energy harvest process, what is the immediate outcome of establishing a transmembrane proton gradient?
In the energy harvest process, what is the immediate outcome of establishing a transmembrane proton gradient?
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What is the role of redox potential in the context of energy storage?
What is the role of redox potential in the context of energy storage?
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During a redox reaction, if molecule 'Xe-' becomes 'X', what process has occurred?
During a redox reaction, if molecule 'Xe-' becomes 'X', what process has occurred?
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What cellular process is Peter Mitchell known for proposing around 1960?
What cellular process is Peter Mitchell known for proposing around 1960?
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Which of the following statements accurately describes the role of NAD+ in cellular respiration?
Which of the following statements accurately describes the role of NAD+ in cellular respiration?
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What is the primary role of the proton gradient established across the inner mitochondrial membrane?
What is the primary role of the proton gradient established across the inner mitochondrial membrane?
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Under anaerobic conditions, which process generates ATP and how many ATP molecules are produced per glucose molecule?
Under anaerobic conditions, which process generates ATP and how many ATP molecules are produced per glucose molecule?
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Which of the following correctly describes the relationship between exergonic and endergonic reactions?
Which of the following correctly describes the relationship between exergonic and endergonic reactions?
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What is the net ATP production from glycolysis alone, before it feeds into respiration or fermentation?
What is the net ATP production from glycolysis alone, before it feeds into respiration or fermentation?
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Which of the following statements regarding the overall reaction of cellular respiration ($C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O$) is most accurate?
Which of the following statements regarding the overall reaction of cellular respiration ($C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O$) is most accurate?
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How does oxidative phosphorylation contribute to ATP production within the cell?
How does oxidative phosphorylation contribute to ATP production within the cell?
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Which of the folllowing best summarizes metabolism?
Which of the folllowing best summarizes metabolism?
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What does the first law of thermodynamics state?
What does the first law of thermodynamics state?
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What is the significance of the second law of thermodynamics in energy transfer?
What is the significance of the second law of thermodynamics in energy transfer?
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Which of the following describes a reason why enzymes are important in metabolic pathways?
Which of the following describes a reason why enzymes are important in metabolic pathways?
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What is the role of ATP in cellular metabolism?
What is the role of ATP in cellular metabolism?
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Which process is primarily responsible for the irreversible fixation of energy in organisms?
Which process is primarily responsible for the irreversible fixation of energy in organisms?
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In what way do living organisms lose energy to their environment?
In what way do living organisms lose energy to their environment?
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Which of the following represents a key concept related to energy capture in living systems?
Which of the following represents a key concept related to energy capture in living systems?
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Which of the following pathways is directly involved in the generation of ATP under aerobic conditions?
Which of the following pathways is directly involved in the generation of ATP under aerobic conditions?
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What is the primary role of ATP in a cell?
What is the primary role of ATP in a cell?
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Which metabolic process is characterized by the use of energy to build complex molecules?
Which metabolic process is characterized by the use of energy to build complex molecules?
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What is the consequence of cellular respiration?
What is the consequence of cellular respiration?
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Which process is an example of catabolism?
Which process is an example of catabolism?
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What is the relationship between Gibbs free energy and the universe?
What is the relationship between Gibbs free energy and the universe?
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How do plants and animals differ in energy conversion?
How do plants and animals differ in energy conversion?
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What type of energy is used for cellular work?
What type of energy is used for cellular work?
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Which of the following best describes metabolism?
Which of the following best describes metabolism?
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What kind of energy loss is inevitable during metabolic processes?
What kind of energy loss is inevitable during metabolic processes?
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What is the purpose of metabolic pathways?
What is the purpose of metabolic pathways?
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What is the primary purpose of glycolysis in cellular respiration?
What is the primary purpose of glycolysis in cellular respiration?
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Which statement correctly describes Gibbs free energy and its change?
Which statement correctly describes Gibbs free energy and its change?
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Which enzyme type is involved in incorporating phosphate groups during glycolysis?
Which enzyme type is involved in incorporating phosphate groups during glycolysis?
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Which of the following correctly represents the relationship between enthalpy, entropy, and Gibbs free energy?
Which of the following correctly represents the relationship between enthalpy, entropy, and Gibbs free energy?
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During the citric acid cycle, how many NADH molecules are produced from one cycle with Acetyl CoA?
During the citric acid cycle, how many NADH molecules are produced from one cycle with Acetyl CoA?
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What happens during the preparation of pyruvate for the citric acid cycle if oxygen is not present?
What happens during the preparation of pyruvate for the citric acid cycle if oxygen is not present?
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What is the primary function of enzymes in metabolic pathways?
What is the primary function of enzymes in metabolic pathways?
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Which process leads to the formation of ATP via substrate-level phosphorylation?
Which process leads to the formation of ATP via substrate-level phosphorylation?
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Which of the following statements correctly describes the role of the electron transport chain?
Which of the following statements correctly describes the role of the electron transport chain?
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Which of these statements about ATP's energy release is accurate?
Which of these statements about ATP's energy release is accurate?
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What is produced as a result of the final step of glycolysis?
What is produced as a result of the final step of glycolysis?
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The process of oxidation-reduction in respiration mainly involves which of the following?
The process of oxidation-reduction in respiration mainly involves which of the following?
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How are enzymes affected by product inhibition?
How are enzymes affected by product inhibition?
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In terms of energy metabolism, what is the catabolic route characterized by?
In terms of energy metabolism, what is the catabolic route characterized by?
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What process describes the movement of ions across a semipermeable membrane driven by electrochemical gradients?
What process describes the movement of ions across a semipermeable membrane driven by electrochemical gradients?
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During oxidative phosphorylation, what roles do the electron transport chain and chemiosmosis play?
During oxidative phosphorylation, what roles do the electron transport chain and chemiosmosis play?
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Which of the following is a product of the citric acid cycle?
Which of the following is a product of the citric acid cycle?
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What is the relationship between glycolysis and the citric acid cycle?
What is the relationship between glycolysis and the citric acid cycle?
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Flashcards
Free Energy
Free Energy
Energy available to do work in a system.
Entropy
Entropy
Measure of disorder or randomness in a system.
First Law of Thermodynamics
First Law of Thermodynamics
Energy cannot be created or destroyed, only transformed.
Second Law of Thermodynamics
Second Law of Thermodynamics
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Role of ATP
Role of ATP
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Glycolysis
Glycolysis
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Krebs Cycle
Krebs Cycle
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Electron Transport Chain
Electron Transport Chain
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Cellular respiration
Cellular respiration
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Metabolism
Metabolism
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ATP energy currency
ATP energy currency
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Anabolism
Anabolism
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Catabolism
Catabolism
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Gibbs free energy
Gibbs free energy
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Energy balance
Energy balance
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Kinetic energy
Kinetic energy
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Heat energy
Heat energy
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Energy loss
Energy loss
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Gibbs Free Energy (G)
Gibbs Free Energy (G)
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ΔG Equation
ΔG Equation
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Spontaneity of Reactions
Spontaneity of Reactions
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Exergonic Reaction
Exergonic Reaction
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Enzymes Function
Enzymes Function
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ATP
ATP
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Substrate-Level Phosphorylation
Substrate-Level Phosphorylation
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Chemiosmosis
Chemiosmosis
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Redox potential
Redox potential
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Electronegativity
Electronegativity
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Electron transport
Electron transport
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NADH
NADH
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Redox reaction
Redox reaction
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Proton gradient
Proton gradient
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ATP synthesis
ATP synthesis
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Fermentation
Fermentation
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Energy yield of fermentation
Energy yield of fermentation
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Overall reaction for cellular respiration
Overall reaction for cellular respiration
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Anabolic vs Catabolic
Anabolic vs Catabolic
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Role of NAD+
Role of NAD+
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ATP synthesis via chemiosmosis
ATP synthesis via chemiosmosis
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Energy Harvest
Energy Harvest
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Glycolysis Steps
Glycolysis Steps
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Kinase Enzyme
Kinase Enzyme
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Isomerase Enzyme
Isomerase Enzyme
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Dehydrogenase Enzyme
Dehydrogenase Enzyme
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Pyruvate
Pyruvate
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Citric Acid Cycle
Citric Acid Cycle
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NADH and FADH2
NADH and FADH2
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Study Notes
Cellular Energetics
- Cellular energetics is the study of how cells produce and use energy.
- It is based on the laws of thermodynamics.
- It relates to the capture, storage, and conversion of energy within cells.
Course Learning Objectives (ILOs)
- Explain free energy and entropy concepts.
- Use thermodynamics to explain enzyme effectiveness in metabolic pathways.
- Detail the function of ATP in coupling energy-releasing and energy-requiring processes.
- Explain the role of oxidation-reduction reactions in generating energy.
- Define glycolysis, the Krebs cycle, electron transport chain, chemiosmosis, oxidative phosphorylation and cellular respiration.
- Identify the location and name the main pathways for ATP production under aerobic and anaerobic conditions.
Bioenergetics
- How cells produce and use energy
- Based on the laws of thermodynamics
- Relates to energy capture (respiration, photosynthesis), storage (ATP, NADH, proton gradient) and conversion (light, chemical, kinetic).
Energy Conservation and Conversion
- First Law of Thermodynamics: Energy cannot be created or destroyed, only converted from one form to another.
- Second Law of Thermodynamics: Every energy transfer increases the entropy (disorder) of the universe. Energy transfer involves loss to the environment via heat.
Energy Conservation & Conversion in Biological Systems
- Living organisms undergo constant energy input to perform work (e.g., movement, growth).
- Organisms lose some energy to the environment as heat
- Some energy becomes irreversibly fixed, like in secondary plant metabolites
Energy Balance (Ecosystem & Individual)
- Energy is continually converted between forms in all individuals, and ecosystems.
- Energy is converted into various forms like heat energy, loss and storage (e.g., ATP).
- The process of energy's metabolic processes are shown in the food chain diagram
Metabolism & ATP
- Metabolism: the sum of all chemical reactions in an organism.
- Anabolism (building up): Energy used to build complex molecules (e.g., protein synthesis from amino acids).
- Catabolism (breaking down): Energy released via the breakdown of complex molecules (e.g., glucose breakdown in glycolysis).
- ATP acts as an energy reservoir in cells, driving cellular work (chemical, transport, movement).
Gibbs Free Energy (G)
- Gibbs free energy (G) is the portion of a system's energy that can perform work.
- ΔG = ΔH - TΔS (where ΔH is enthalpy, T is temperature, and ΔS is entropy).
- A negative ΔG indicates a spontaneous process.
Chemical Reactions and Energy
- Exergonic reactions: Release energy (ΔG < 0).
- Endergonic reactions: Require energy input (ΔG > 0).
- Energy released from exergonic reactions can be utilized to drive endergonic reactions.
- Enzymes accelerate chemical reactions by lowering activation energy.
Enzymes
- Enzymes, often pH-dependent, regulate metabolic pathways by lowering activation energies, and are regulated by gene expression and modification.
- The end-product often inhibits further reaction.
ATP as Energy Currency
- ATP (adenosine triphosphate) releases energy when an inorganic phosphate (Pi) is cleaved off, yielding ADP (adenosine diphosphate).
- The stored chemical energy comes from the negative charges repelling each other.
How ATP is Formed
- Substrate-level phosphorylation: Transfer of phosphate group directly from a substrate molecule to ADP (e.g., glycolysis).
- Oxidative phosphorylation (chemiosmosis): ATP synthase utilizes the H+ gradient across a membrane.
Redox Potential & Electron Transport Chain
- Redox potential reflects the relative tendency of substances to gain or lose electrons.
- Relocating electrons from a substance with low electronegativity (e.g., a sugar) to a substance with high electronegativity (e.g., oxygen) releases energy.
NADH
- NADH (nicotinamide adenine dinucleotide) acts as an electron carrier.
- Many NADH molecules are produced during food breakdown.
Energy Harvest (Chemiosmosis)
- Energy is stored in a transmembrane proton (H+) gradient.
- This gradient is used to produce ATP.
- Movement of H+ ions across a membrane is associated with electron transport.
Energy Harvest: From Electrons to ATP
- NADH is used in redox reactions to transfer protons with electrons during the electron transport chain.
- The proton gradient powers ATP production.
Cellular Respiration
- Cellular respiration is a metabolic process to obtain energy by oxidizing nutrients and releasing waste.
- It involves pathways concerned with energy metabolism and proceeds across cellular compartments.
- It involves three main stages: glycolysis, the citric acid cycle, and oxidative phosphorylation.
Glycolysis
- Glycolysis is the breakdown of glucose into pyruvate.
- It occurs in the cytoplasm and yields two ATP.
- It produces NADH, an electron carrier.
Preparing Pyruvate for Citric Acid Cycle
- Occurs in the mitochondrion.
- It produces Acetyl CoA, releasing CO2 and generating NADH.
Citric Acid Cycle
- Also called the TCA (tricarboxylic acid) cycle, or the Krebs cycle.
- It is a cyclical set of reactions in the mitochondrion.
- It uses Acetyl CoA and produces NADH, FADH2, and ATP (or GTP).
Oxidative Phosphorylation
- Occurs in the inner mitochondrial membrane and generates a substantial number of ATP using an electron transport chain.
- Chemiosmosis occurs to establish the H+ proton gradient
Electron Transport Chain & ATP Synthase
- The electron transport chain utilizes electrons carried by NADH and FADH2 to generate an electrochemical proton (H+) gradient across a membrane.
- ATP synthase is the enzyme that produces ATP using the potential energy stored from the H+ gradient.
Fermentation
- A metabolic process to produce ATP in the absence of oxygen.
- Types include alcohol and lactic acid fermentation and yield only two ATP per glucose molecule.
Overall Cellular Respiration
- Energy balance involves oxidative phosphorylation producing substantially more ATP per glucose molecule than the overall energy gained from fermentation in the lack of oxygen.
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Description
Test your understanding of the key concepts of cellular respiration, including the roles of NADH and proton gradients in energy production. This quiz covers fundamental principles, redox reactions, and the significance of membrane integrity in chemiosmosis. Perfect for students studying biochemistry or related fields.