Biochemistry Cellular Respiration Quiz
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Questions and Answers

What is the fundamental principle behind energy release when relocating electrons from sugars to oxygen?

  • Sugars are strongly electronegative, attracting electrons from oxygen.
  • The difference in electronegativity between sugars and oxygen. (correct)
  • The similar electronegativity between water and oxygen, causing an imbalance.
  • Oxygen's weak electronegativity forces electrons to be released by sugars.
  • Which of the following is NOT a primary function of NADH in cellular metabolism?

  • Directly synthesizing ATP without any intermediate steps. (correct)
  • Acting as an electron currency to transfer electrons.
  • Facilitating redox reactions by becoming oxidized.
  • Serving as a crucial component in energy harvest processes.
  • What role does electronegativity play in redox reactions?

  • It determines the rate at which molecules degrade.
  • It dictates whether a molecule will act as an enzyme.
  • It establishes the physical size of molecules.
  • It gauges an atom's affinity for electrons. (correct)
  • Why are intact membranes essential for chemiosmosis?

    <p>To create a barrier that allows for the establishment of a proton gradient. (C)</p> Signup and view all the answers

    In the energy harvest process, what is the immediate outcome of establishing a transmembrane proton gradient?

    <p>It stores potential energy that can be used to synthesize ATP. (A)</p> Signup and view all the answers

    What is the role of redox potential in the context of energy storage?

    <p>It represents the potential energy stored in electron transfer reactions. (B)</p> Signup and view all the answers

    During a redox reaction, if molecule 'Xe-' becomes 'X', what process has occurred?

    <p>Xe- has been oxidized. (B)</p> Signup and view all the answers

    What cellular process is Peter Mitchell known for proposing around 1960?

    <p>Chemiosmosis (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of NAD+ in cellular respiration?

    <p>NAD+ captures electrons during redox reactions and delivers them to the electron transport chain. (A)</p> Signup and view all the answers

    What is the primary role of the proton gradient established across the inner mitochondrial membrane?

    <p>To store energy that can be used for the synthesis of ATP by ATP synthase. (B)</p> Signup and view all the answers

    Under anaerobic conditions, which process generates ATP and how many ATP molecules are produced per glucose molecule?

    <p>Fermentation, producing 2 ATP. (A)</p> Signup and view all the answers

    Which of the following correctly describes the relationship between exergonic and endergonic reactions?

    <p>Exergonic reactions release energy that can be used to drive endergonic reactions. (D)</p> Signup and view all the answers

    What is the net ATP production from glycolysis alone, before it feeds into respiration or fermentation?

    <p>2 ATP (A)</p> Signup and view all the answers

    Which of the following statements regarding the overall reaction of cellular respiration ($C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O$) is most accurate?

    <p>The reaction is exergonic, releasing energy in the form of ATP. (D)</p> Signup and view all the answers

    How does oxidative phosphorylation contribute to ATP production within the cell?

    <p>By utilizing the energy from the electron transport chain to establish a proton gradient that drives ATP synthase. (A)</p> Signup and view all the answers

    Which of the folllowing best summarizes metabolism?

    <p>The totality of an organism’s chemical reactions, consisting of catabolic and anabolic pathways. (A)</p> Signup and view all the answers

    What does the first law of thermodynamics state?

    <p>Energy cannot be created or destroyed. (A)</p> Signup and view all the answers

    What is the significance of the second law of thermodynamics in energy transfer?

    <p>It states that some energy will always increase disorder. (A)</p> Signup and view all the answers

    Which of the following describes a reason why enzymes are important in metabolic pathways?

    <p>They reduce the activation energy needed for reactions. (C)</p> Signup and view all the answers

    What is the role of ATP in cellular metabolism?

    <p>To serve as an energy carrier that couples energy-releasing and energy-requiring processes. (A)</p> Signup and view all the answers

    Which process is primarily responsible for the irreversible fixation of energy in organisms?

    <p>Photosynthesis. (B)</p> Signup and view all the answers

    In what way do living organisms lose energy to their environment?

    <p>As heat, during energy transformations. (A)</p> Signup and view all the answers

    Which of the following represents a key concept related to energy capture in living systems?

    <p>Energy is captured mainly through respiration and photosynthesis. (C)</p> Signup and view all the answers

    Which of the following pathways is directly involved in the generation of ATP under aerobic conditions?

    <p>Electron transport chain. (B)</p> Signup and view all the answers

    What is the primary role of ATP in a cell?

    <p>To act as an energy reservoir (C)</p> Signup and view all the answers

    Which metabolic process is characterized by the use of energy to build complex molecules?

    <p>Anabolism (D)</p> Signup and view all the answers

    What is the consequence of cellular respiration?

    <p>Release of waste products (A)</p> Signup and view all the answers

    Which process is an example of catabolism?

    <p>Breakdown of glucose in glycolysis (D)</p> Signup and view all the answers

    What is the relationship between Gibbs free energy and the universe?

    <p>The universe equals the sum of system and surroundings (C)</p> Signup and view all the answers

    How do plants and animals differ in energy conversion?

    <p>Plants convert solar energy through photosynthesis (B)</p> Signup and view all the answers

    What type of energy is used for cellular work?

    <p>Chemical energy (B)</p> Signup and view all the answers

    Which of the following best describes metabolism?

    <p>The totality of an organism's chemical reactions (D)</p> Signup and view all the answers

    What kind of energy loss is inevitable during metabolic processes?

    <p>Thermal energy (D)</p> Signup and view all the answers

    What is the purpose of metabolic pathways?

    <p>To facilitate enzyme reactions and energy transformations (B)</p> Signup and view all the answers

    What is the primary purpose of glycolysis in cellular respiration?

    <p>To convert glucose to pyruvate (B)</p> Signup and view all the answers

    Which statement correctly describes Gibbs free energy and its change?

    <p>A reaction occurs spontaneously if the change in Gibbs free energy is negative. (D)</p> Signup and view all the answers

    Which enzyme type is involved in incorporating phosphate groups during glycolysis?

    <p>Kinase (A)</p> Signup and view all the answers

    Which of the following correctly represents the relationship between enthalpy, entropy, and Gibbs free energy?

    <p>$ ext{ΔG} = ext{ΔH} - T ext{ΔS}$ (B)</p> Signup and view all the answers

    During the citric acid cycle, how many NADH molecules are produced from one cycle with Acetyl CoA?

    <p>3 NADH (A)</p> Signup and view all the answers

    What happens during the preparation of pyruvate for the citric acid cycle if oxygen is not present?

    <p>The reaction does not proceed (D)</p> Signup and view all the answers

    What is the primary function of enzymes in metabolic pathways?

    <p>Enzymes lower the activation energy required for reactions. (A)</p> Signup and view all the answers

    Which process leads to the formation of ATP via substrate-level phosphorylation?

    <p>Glycolysis with phosphoglycerokinase (A)</p> Signup and view all the answers

    Which of the following statements correctly describes the role of the electron transport chain?

    <p>It generates H+ gradients for ATP synthesis (D)</p> Signup and view all the answers

    Which of these statements about ATP's energy release is accurate?

    <p>When ATP is cleaved, energy is released due to repulsion between phosphate groups. (C)</p> Signup and view all the answers

    What is produced as a result of the final step of glycolysis?

    <p>Pyruvate (A)</p> Signup and view all the answers

    The process of oxidation-reduction in respiration mainly involves which of the following?

    <p>Transfer of electrons and protons (B)</p> Signup and view all the answers

    How are enzymes affected by product inhibition?

    <p>End products can inhibit enzyme function by negative feedback. (C)</p> Signup and view all the answers

    In terms of energy metabolism, what is the catabolic route characterized by?

    <p>Involves energy release (B)</p> Signup and view all the answers

    What process describes the movement of ions across a semipermeable membrane driven by electrochemical gradients?

    <p>Chemiosmosis (A)</p> Signup and view all the answers

    During oxidative phosphorylation, what roles do the electron transport chain and chemiosmosis play?

    <p>Chemiosmosis synthesizes ATP by utilizing energy stored in the electron transport chain. (B)</p> Signup and view all the answers

    Which of the following is a product of the citric acid cycle?

    <p>NADH (C)</p> Signup and view all the answers

    What is the relationship between glycolysis and the citric acid cycle?

    <p>Glycolysis converts glucose to pyruvate, feeding into the citric acid cycle (B)</p> Signup and view all the answers

    Flashcards

    Free Energy

    Energy available to do work in a system.

    Entropy

    Measure of disorder or randomness in a system.

    First Law of Thermodynamics

    Energy cannot be created or destroyed, only transformed.

    Second Law of Thermodynamics

    Energy transfers increase the universe's entropy.

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    Role of ATP

    ATP couples energy-releasing and energy-requiring processes.

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    Glycolysis

    Process that breaks down glucose to generate ATP.

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    Krebs Cycle

    Series of reactions that produce electron carriers for the electron transport chain.

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    Electron Transport Chain

    Sequence of proteins that transfer electrons, generating ATP via chemiosmosis.

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    Cellular respiration

    Metabolic process to obtain energy by oxidizing nutrients and releasing waste.

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    Metabolism

    The totality of an organism's chemical reactions, including anabolism and catabolism.

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    ATP energy currency

    Adenosine triphosphate (ATP) acts as the energy reservoir for cellular work.

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    Anabolism

    The process of building complex molecules from simpler ones using energy.

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    Catabolism

    The process of breaking down complex molecules to release energy.

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    Gibbs free energy

    The energy needed for a system to do work, considering entropy and its surroundings.

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    Energy balance

    The equilibrium between energy intake and energy expenditure in an ecosystem or individual.

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    Kinetic energy

    Energy that a body possesses due to its motion.

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    Heat energy

    Energy that comes from the movement of atoms and molecules, often a byproduct of metabolism.

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    Energy loss

    The process where energy is dissipated, often as heat, during metabolic processes.

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    Gibbs Free Energy (G)

    The portion of a system's energy that can perform work.

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    ΔG Equation

    ΔG = ΔH - TΔS; change in free energy calculation.

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    Spontaneity of Reactions

    A process occurs spontaneously if ΔG is negative.

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    Exergonic Reaction

    A reaction that releases energy and has a negative ΔG.

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    Enzymes Function

    Biological catalysts that lower activation energy of reactions.

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    ATP

    Adenosine-triphosphate, an energy currency releasing energy when Pi is cleaved.

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    Substrate-Level Phosphorylation

    Direct transfer of phosphate to ADP to form ATP during glycolysis.

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    Chemiosmosis

    Movement of ions down an electrochemical gradient to generate ATP.

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    Redox potential

    The measure of an atom's ability to gain or lose electrons in a chemical reaction.

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    Electronegativity

    A measure of an atom's ability to attract electrons in a bond.

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    Electron transport

    A series of reactions where electrons are transferred from one molecule to another.

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    NADH

    An electron carrier that stores energy from food breakdown.

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    Redox reaction

    A chemical reaction involving the transfer of electrons between two species.

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    Proton gradient

    A difference in proton concentration across a membrane that stores energy.

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    ATP synthesis

    The process of generating ATP from ADP and inorganic phosphate, usually using energy from chemiosmosis.

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    Fermentation

    Anaerobic process that converts glucose to 2 ATP.

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    Energy yield of fermentation

    Fermentation produces only 2 ATP per glucose compared to 32 ATP from respiration.

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    Overall reaction for cellular respiration

    Chemical equation: C6H12O6 + 6 O2 → 6 CO2 + 6 H2O.

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    Anabolic vs Catabolic

    Anabolic builds molecules; catabolic breaks them down in metabolism.

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    Role of NAD+

    NAD+ captures electrons during redox reactions and forms NADH.

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    ATP synthesis via chemiosmosis

    H+ flows back through ATP synthase to produce ATP from ADP.

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    Energy Harvest

    Process of converting electrons into ATP via REDOX reactions.

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    Glycolysis Steps

    Series of steps that convert glucose into two pyruvate molecules while generating ATP.

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    Kinase Enzyme

    An enzyme that adds phosphate groups to molecules, often using ATP.

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    Isomerase Enzyme

    An enzyme that converts one isomer into another, rearranging the molecule's structure.

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    Dehydrogenase Enzyme

    An enzyme that facilitates oxidation reactions by transferring electrons to NAD+.

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    Pyruvate

    The end product of glycolysis, which can enter the citric acid cycle or fermentation.

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    Citric Acid Cycle

    Also known as TCA or Krebs cycle; processes Acetyl CoA to produce energy carriers.

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    NADH and FADH2

    Electron carriers produced in glycolysis and citric acid cycle for ATP generation.

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    Study Notes

    Cellular Energetics

    • Cellular energetics is the study of how cells produce and use energy.
    • It is based on the laws of thermodynamics.
    • It relates to the capture, storage, and conversion of energy within cells.

    Course Learning Objectives (ILOs)

    • Explain free energy and entropy concepts.
    • Use thermodynamics to explain enzyme effectiveness in metabolic pathways.
    • Detail the function of ATP in coupling energy-releasing and energy-requiring processes.
    • Explain the role of oxidation-reduction reactions in generating energy.
    • Define glycolysis, the Krebs cycle, electron transport chain, chemiosmosis, oxidative phosphorylation and cellular respiration.
    • Identify the location and name the main pathways for ATP production under aerobic and anaerobic conditions.

    Bioenergetics

    • How cells produce and use energy
    • Based on the laws of thermodynamics
    • Relates to energy capture (respiration, photosynthesis), storage (ATP, NADH, proton gradient) and conversion (light, chemical, kinetic).

    Energy Conservation and Conversion

    • First Law of Thermodynamics: Energy cannot be created or destroyed, only converted from one form to another.
    • Second Law of Thermodynamics: Every energy transfer increases the entropy (disorder) of the universe. Energy transfer involves loss to the environment via heat.

    Energy Conservation & Conversion in Biological Systems

    • Living organisms undergo constant energy input to perform work (e.g., movement, growth).
    • Organisms lose some energy to the environment as heat
    • Some energy becomes irreversibly fixed, like in secondary plant metabolites

    Energy Balance (Ecosystem & Individual)

    • Energy is continually converted between forms in all individuals, and ecosystems.
    • Energy is converted into various forms like heat energy, loss and storage (e.g., ATP).
    • The process of energy's metabolic processes are shown in the food chain diagram

    Metabolism & ATP

    • Metabolism: the sum of all chemical reactions in an organism.
      • Anabolism (building up): Energy used to build complex molecules (e.g., protein synthesis from amino acids).
      • Catabolism (breaking down): Energy released via the breakdown of complex molecules (e.g., glucose breakdown in glycolysis).
    • ATP acts as an energy reservoir in cells, driving cellular work (chemical, transport, movement).

    Gibbs Free Energy (G)

    • Gibbs free energy (G) is the portion of a system's energy that can perform work.
    • ΔG = ΔH - TΔS (where ΔH is enthalpy, T is temperature, and ΔS is entropy).
      • A negative ΔG indicates a spontaneous process.

    Chemical Reactions and Energy

    • Exergonic reactions: Release energy (ΔG < 0).
    • Endergonic reactions: Require energy input (ΔG > 0).
      • Energy released from exergonic reactions can be utilized to drive endergonic reactions.
    • Enzymes accelerate chemical reactions by lowering activation energy.

    Enzymes

    • Enzymes, often pH-dependent, regulate metabolic pathways by lowering activation energies, and are regulated by gene expression and modification.
      • The end-product often inhibits further reaction.

    ATP as Energy Currency

    • ATP (adenosine triphosphate) releases energy when an inorganic phosphate (Pi) is cleaved off, yielding ADP (adenosine diphosphate).
    • The stored chemical energy comes from the negative charges repelling each other.

    How ATP is Formed

    • Substrate-level phosphorylation: Transfer of phosphate group directly from a substrate molecule to ADP (e.g., glycolysis).
    • Oxidative phosphorylation (chemiosmosis): ATP synthase utilizes the H+ gradient across a membrane.

    Redox Potential & Electron Transport Chain

    • Redox potential reflects the relative tendency of substances to gain or lose electrons.
    • Relocating electrons from a substance with low electronegativity (e.g., a sugar) to a substance with high electronegativity (e.g., oxygen) releases energy.

    NADH

    • NADH (nicotinamide adenine dinucleotide) acts as an electron carrier.
    • Many NADH molecules are produced during food breakdown.

    Energy Harvest (Chemiosmosis)

    • Energy is stored in a transmembrane proton (H+) gradient.
    • This gradient is used to produce ATP.
    • Movement of H+ ions across a membrane is associated with electron transport.

    Energy Harvest: From Electrons to ATP

    • NADH is used in redox reactions to transfer protons with electrons during the electron transport chain.
    • The proton gradient powers ATP production.

    Cellular Respiration

    • Cellular respiration is a metabolic process to obtain energy by oxidizing nutrients and releasing waste.
    • It involves pathways concerned with energy metabolism and proceeds across cellular compartments.
    • It involves three main stages: glycolysis, the citric acid cycle, and oxidative phosphorylation.

    Glycolysis

    • Glycolysis is the breakdown of glucose into pyruvate.
    • It occurs in the cytoplasm and yields two ATP.
    • It produces NADH, an electron carrier.

    Preparing Pyruvate for Citric Acid Cycle

    • Occurs in the mitochondrion.
    • It produces Acetyl CoA, releasing CO2 and generating NADH.

    Citric Acid Cycle

    • Also called the TCA (tricarboxylic acid) cycle, or the Krebs cycle.
    • It is a cyclical set of reactions in the mitochondrion.
    • It uses Acetyl CoA and produces NADH, FADH2, and ATP (or GTP).

    Oxidative Phosphorylation

    • Occurs in the inner mitochondrial membrane and generates a substantial number of ATP using an electron transport chain.
    • Chemiosmosis occurs to establish the H+ proton gradient

    Electron Transport Chain & ATP Synthase

    • The electron transport chain utilizes electrons carried by NADH and FADH2 to generate an electrochemical proton (H+) gradient across a membrane.
    • ATP synthase is the enzyme that produces ATP using the potential energy stored from the H+ gradient.

    Fermentation

    • A metabolic process to produce ATP in the absence of oxygen.
    • Types include alcohol and lactic acid fermentation and yield only two ATP per glucose molecule.

    Overall Cellular Respiration

    • Energy balance involves oxidative phosphorylation producing substantially more ATP per glucose molecule than the overall energy gained from fermentation in the lack of oxygen.

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