Biochemistry: ATP and Metabolic Reactions
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Questions and Answers

What is produced when NAD+ undergoes reduction in the metabolic reaction involving ethanol?

  • NADH and H+ (correct)
  • NADH and acetaldehyde
  • FADH and H2O
  • NADH and CO2
  • What type of metabolic reactions requires the coenzyme NAD+?

  • Reactions producing nitrogenous bases
  • Reactions producing carbon-oxygen double bonds (correct)
  • Reactions producing carbon-hydrogen bonds
  • Reactions producing amino acids
  • Which coenzyme is formed when FAD undergoes reduction?

  • FAD
  • FADH
  • FADH2 (correct)
  • FADH3
  • In the oxidation of ethanol to ethanal, which enzyme is responsible for the reaction?

    <p>Alcohol dehydrogenase (B)</p> Signup and view all the answers

    What acts as the electron acceptor in the oxidation of alcohols to aldehydes and ketones?

    <p>NAD+ (B)</p> Signup and view all the answers

    How many ATP molecules can be hydrolyzed in one second during anabolic processes?

    <p>1 to 2 million (B)</p> Signup and view all the answers

    What is the maximum amount of ATP hydrolyzed in one day relative to body mass?

    <p>As much as our body mass (B)</p> Signup and view all the answers

    Which process provides energy to regenerate ATP in cells?

    <p>Catabolic reactions (B)</p> Signup and view all the answers

    How much energy in kcal/mole is used to make ATP from ADP and Pi?

    <p>7.3 kcal/mole (D)</p> Signup and view all the answers

    What type of reaction is used in the hydrolysis of ATP?

    <p>Catabolic reaction (C)</p> Signup and view all the answers

    What is the energy change in kcal/mole when ATP is formed from ADP and Pi?

    <p>7.3 kcal/mole (C)</p> Signup and view all the answers

    What role does ATP play in cellular metabolism?

    <p>It links energy-releasing and energy-requiring reactions (D)</p> Signup and view all the answers

    What happens during anabolic processes in the context of ATP?

    <p>ATP is synthesized using energy (D)</p> Signup and view all the answers

    What happens to FAD during the conversion of succinate to fumarate?

    <p>FAD is reduced to FADH2 (B)</p> Signup and view all the answers

    What type of coenzyme is involved in the oxidation of succinate?

    <p>FAD (D)</p> Signup and view all the answers

    What is the primary function of coenzyme A (CoA) in metabolic reactions?

    <p>To prepare acyl groups for enzyme reactions (B)</p> Signup and view all the answers

    During the formation of acetyl CoA, what is the fate of the thiol group in Coenzyme A?

    <p>It bonds to the acetyl group (B)</p> Signup and view all the answers

    What is produced when succinate is converted to fumarate in the citric acid cycle?

    <p>FADH2 (D)</p> Signup and view all the answers

    Which of the following components makes up coenzyme A?

    <p>Pantothenic acid, ADP, and aminoethanethiol (A)</p> Signup and view all the answers

    In which reaction is coenzyme A not involved?

    <p>Conversion of succinate to fumarate (C)</p> Signup and view all the answers

    What is the outcome of the addition of an acetyl group to coenzyme A?

    <p>CoA forms a thioester bond (B)</p> Signup and view all the answers

    What is the primary role of NAD+ in the oxidation of glyceraldehyde-3-phosphate?

    <p>NAD+ is reduced to NADH during the reaction. (B)</p> Signup and view all the answers

    What is produced when a phosphate group is transferred from 1,3-bisphosphoglycerate to ADP?

    <p>ATP (B)</p> Signup and view all the answers

    What occurs during the isomerization step of glycolysis involving 3-phosphoglycerate?

    <p>The phosphate group is moved from carbon 3 to carbon 2. (B)</p> Signup and view all the answers

    How many molecules of ATP are produced during the transfer of phosphate from phosphoenolpyruvate to ADP?

    <p>Two (C)</p> Signup and view all the answers

    What is the end product of the reaction catalyzed by pyruvate kinase?

    <p>2 Pyruvate (C)</p> Signup and view all the answers

    Which compound is formed through dehydration during glycolysis?

    <p>Phosphoenolpyruvate (C)</p> Signup and view all the answers

    What is the significance of the oxidation and phosphorylation reaction involving glyceraldehyde-3-phosphate?

    <p>It forms 1,3-bisphosphoglycerate, a high-energy compound. (D)</p> Signup and view all the answers

    What type of reaction takes place when 2,3-bisphosphoglycerate is converted into 3-phosphoglycerate?

    <p>Isomerization (D)</p> Signup and view all the answers

    What happens to pyruvate under aerobic conditions?

    <p>It is oxidized and further processed in mitochondria. (A)</p> Signup and view all the answers

    What role does NAD+ play during the oxidation of pyruvate?

    <p>It is reduced to form NADH. (D)</p> Signup and view all the answers

    What is the product formed when pyruvate is oxidized?

    <p>Acetyl CoA. (C)</p> Signup and view all the answers

    What is produced as a byproduct when pyruvate is oxidized?

    <p>NADH. (B)</p> Signup and view all the answers

    What is the significance of acetyl CoA in metabolic pathways?

    <p>It is an important intermediate in metabolic reactions. (D)</p> Signup and view all the answers

    Which molecule is involved in the conversion of pyruvate to acetyl CoA?

    <p>CoA. (D)</p> Signup and view all the answers

    What is the main purpose of the process that converts glucose to pyruvate?

    <p>To generate energy in the form of ATP. (D)</p> Signup and view all the answers

    What byproduct is released during the oxidation of pyruvate?

    <p>Carbon dioxide. (C)</p> Signup and view all the answers

    What is the primary function of electron transport in cellular respiration?

    <p>Pass electrons to oxygen and form water (D)</p> Signup and view all the answers

    How many NADH molecules are produced from the complete oxidation of one glucose molecule?

    <p>10 (B)</p> Signup and view all the answers

    In which part of cellular respiration does oxidative phosphorylation primarily occur?

    <p>Electron transport chain (C)</p> Signup and view all the answers

    What is the yield of GTP molecules during the citric acid cycle from one glucose molecule?

    <p>1 (A)</p> Signup and view all the answers

    What is the yield of ATP molecules from one glucose molecule through glycolysis?

    <p>2 (A)</p> Signup and view all the answers

    Which component of the mitochondria surrounds the matrix?

    <p>Inner membrane (A)</p> Signup and view all the answers

    What role do hydrogen ions play during electron transport?

    <p>They create a proton gradient across the membrane (B)</p> Signup and view all the answers

    Which of the following processes uses the energy from electron transport to synthesize ATP?

    <p>Oxidative phosphorylation (B)</p> Signup and view all the answers

    Flashcards

    ATP Hydrolysis Rate

    In active cells, 1 to 2 million ATP molecules are hydrolyzed per second.

    ATP Regeneration

    Catabolic reactions provide energy to regenerate ATP from ADP and inorganic phosphate (Pi).

    ATP Energy Storage

    ATP stores energy used in anabolic reactions.

    Energy for ATP Synthesis

    Catabolic reactions in food provide the energy for creating ATP.

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    ATP Amount in Cells

    Only about 1 gram of ATP is present in cells at any given time, but a large amount can be hydrolyzed in a day.

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    ATP Production Process

    Energy transformations linking energy-releasing reactions with energy-requiring reactions.

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    ATP Structure

    ATP (Adenosine Triphosphate) is a molecule with 3 phosphate groups. A significant amount of energy is stored in the phosphate bonds.

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    ATP Role in Anabolism

    ATP provides energy needed for anabolic reactions, which build up complex molecules, such as proteins.

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    NAD+ reduction

    NAD+ is reduced to NADH and H+ when it gains electrons (a reducing agent) during metabolic reactions involving oxidation of alcohols like ethanol to aldehydes and ketones

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    NAD+ Coenzyme's Role

    NAD+ plays a key role in metabolic reactions by facilitating the formation of carbon-oxygen double bonds.

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    Ethanol Oxidation

    Ethanol is oxidized to acetaldehyde (ethanal) and NAD+ is reduced to NADH during a metabolic reaction .

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    FAD coenzyme

    FAD is a coenzyme that contains ADP and riboflavin, crucial for redox reactions, and is reduced to FADH2

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    FAD Reduction

    FAD is reduced when it accepts two hydrogen atoms (2H+), changing to FADH2, during a redox reaction

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    FAD's role in oxidation

    FAD accepts hydrogen during a reaction that converts a carbon-carbon single bond to a double bond.

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    Succinate to Fumarate

    The conversion of succinate to fumarate in the citric acid cycle, involving FAD as a coenzyme.

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    Coenzyme A components

    Coenzyme A is composed of pantothenic acid, phosphorylated ADP, and aminoethanethiol.

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    Coenzyme A function

    Coenzyme A prepares acyl groups (like acetyl) for enzyme reactions, especially linking acetyl group to enzymes.

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    Coenzyme A reactive group

    The reactive part of coenzyme A is the thiol (¬SH) group.

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    Acetyl CoA formation

    A two-carbon acetyl group bonds to the thiol group in CoA, forming the energy-rich acetyl CoA.

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    Acetyl group addition

    The addition of an acetyl group to coenzyme A results in a thioester bond formation.

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    Oxidation or reduction with acetyl group addition

    Coenzyme A gets reduced (gains electrons) when an acetyl group is added.

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    Electron Transport

    The process where electrons from NADH and FADH2 are passed along a chain of carriers, releasing energy used to make ATP.

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    Oxidative Phosphorylation

    The process where ATP is synthesized from ADP and Pi using the energy released during electron transport.

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    Mitochondrion

    The organelle where electron transport and oxidative phosphorylation take place.

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    Inner Membrane

    The membrane within a mitochondrion where electron carriers are embedded.

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    Outer Membrane

    The outer layer of a mitochondrion.

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    Intermembrane Space

    The space between the inner and outer membranes of a mitochondrion.

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    Matrix

    The innermost compartment of a mitochondrion where the citric acid cycle takes place.

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    ATP Yield

    The total amount of ATP produced from the complete oxidation of one glucose molecule.

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    Glyceraldehyde-3-phosphate Oxidation

    The aldehyde group of glyceraldehyde-3-phosphate is oxidized to a carboxyl group, while NAD+ is reduced to NADH. A phosphate group (Pi) is added to the carboxyl group to form 1,3-bisphosphoglycerate.

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    What is the role of NAD+ in glycolysis?

    NAD+ is a coenzyme that accepts electrons during the oxidation of glyceraldehyde-3-phosphate, becoming reduced to NADH.

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    1,3-Bisphosphoglycerate

    A high-energy compound formed by the oxidation of glyceraldehyde-3-phosphate, containing two phosphate groups.

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    Phosphate Transfer in Glycolysis

    A phosphate group from 1,3-bisphosphoglycerate is transferred to ADP, producing ATP and 3-phosphoglycerate.

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    Isomerization in Glycolysis

    3-phosphoglycerate is converted into 2-phosphoglycerate by moving the phosphate group from carbon 3 to carbon 2.

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    Dehydration in Glycolysis

    2-phosphoglycerate loses a water molecule to form phosphoenolpyruvate, gaining a high-energy phosphate bond.

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    Phosphoenolpyruvate

    A high-energy compound formed through dehydration, containing a phosphate group with a high potential energy.

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    Final Phosphate Transfer

    A phosphate group from phosphoenolpyruvate is transferred to ADP, generating ATP and pyruvate.

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    Pyruvate's Fate in Aerobic Conditions

    When oxygen is plentiful, pyruvate, a product of glycolysis, moves into the mitochondria to undergo further oxidation.

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    Pyruvate Oxidation

    Within the mitochondria, pyruvate is oxidized, losing a carbon atom as carbon dioxide (CO2).

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    Acetyl CoA: Important Intermediate

    Acetyl CoA is a crucial intermediate in many metabolic pathways, including the citric acid cycle (Krebs cycle).

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    Glucose Degradation in Glycolysis

    Glucose, obtained from the digestion of polysaccharides, is broken down in glycolysis to pyruvate.

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    Role of Oxygen in Cell Respiration

    Oxygen plays a crucial role in aerobic respiration, enabling the complete breakdown of glucose and yielding a greater energy output (ATP).

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    Aerobic vs. Anaerobic Respiration

    Aerobic respiration occurs in the presence of oxygen and produces far more ATP than anaerobic respiration, which happens in the absence of oxygen.

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    Study Notes

    Introduction to General, Organic, and Biological Chemistry

    • This book introduces general, organic, and biological chemistry.

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    Description

    Test your knowledge on the role of ATP and NAD+ in metabolic reactions. This quiz covers various biochemical processes including the oxidation of ethanol and ATP hydrolysis. Explore the significance of coenzymes and energy transformations in cellular metabolism.

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