Biochemical Techniques Quiz
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Questions and Answers

What is the primary purpose of biochemical techniques?

  • To separate mixtures of substances based on their affinities
  • To analyze the functions of biomolecules like proteins and nucleic acids (correct)
  • To study the history of biochemistry
  • To explore the latest research developments in the field of genetics

Which technique is commonly used for separating mixtures based on their affinities?

  • PCR
  • Spectrophotometry
  • Centrifugation
  • Chromatography (correct)

In centrifugation, particles are separated from a solution based on their:

  • Color
  • Pressure
  • Temperature
  • Size, shape, density, viscosity, and rotor speed (correct)

What is one common application of biochemical techniques in modern research?

<p>Studying the genetic material and its functions (C)</p> Signup and view all the answers

Which technique is used to amplify a segment of DNA in molecular biology?

<p>Polymerase chain reaction (PCR) (A)</p> Signup and view all the answers

What is the major difference between differential centrifugation and density-gradient centrifugation?

<p>Separation based on density vs. separation based on size (A)</p> Signup and view all the answers

Which type of electrophoresis involves the movement of charged particles under an electric field?

<p>Moving boundary electrophoresis (A)</p> Signup and view all the answers

What does spectrophotometry measure in a solution?

<p>Concentration of solutes by light absorption (C)</p> Signup and view all the answers

Who was the first to identify DNA as a distinct molecule?

<p>Oswald Avery (A)</p> Signup and view all the answers

Which scientist demonstrated that the enzyme urease was a pure protein and crystallized it?

<p>James B. Sumner (A)</p> Signup and view all the answers

In what year did James Watson and Francis Crick derive the three-dimensional and double-helical model of DNA?

<p>1953 (C)</p> Signup and view all the answers

What event marked the starting point for the birth of Biochemistry according to the text?

<p>The demonstration that fermentation results from enzymes in yeast (B)</p> Signup and view all the answers

Flashcards

What is the primary purpose of biochemical techniques?

To analyze the functions of biomolecules like proteins and nucleic acids.

What is chromatography used for?

A technique used to separate mixtures based on their affinities for a stationary phase.

How does centrifugation work?

In centrifugation, particles are separated based on their size, shape, density, viscosity, and rotor speed.

What is one common application of biochemical techniques in modern research?

Studying the genetic material and its functions.

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What is PCR (Polymerase Chain Reaction)?

A technique used to amplify a segment of DNA in molecular biology. It involves multiple cycles of DNA replication.

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What is the difference between differential and density-gradient centrifugation?

Differential centrifugation separates particles based on size, while density-gradient centrifugation separates based on density.

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What is moving boundary electrophoresis?

A type of electrophoresis involving the movement of charged particles under an electric field.

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What does spectrophotometry measure?

Spectrophotometry measures the concentration of solutes in a solution by measuring the amount of light absorbed by the solution.

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Who was the first to identify DNA as a distinct molecule?

Oswald Avery was the first to identify Deoxyribonucleic acid or DNA as a distinct molecule.

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Who was the first to crystallize an enzyme?

James B. Sumner demonstrated that the enzyme urease was a pure protein and crystallized it.

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When did Watson and Crick discover the double-helix structure of DNA?

In 1953, James Watson and Francis Crick derived the three-dimensional and double-helical model of DNA.

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What event marked the starting point for the birth of Biochemistry?

The demonstration that fermentation results from enzymes in yeast.

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