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Bioassay of Digitalis: Injection of Sample Dilutions

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Bioassay of Digitalis: Injection of Sample Dilutions

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Questions and Answers

What is the primary purpose of adding dilute formaldehyde to each tube after incubation in the bioassay of antibiotics?

To promote the diffusion of antibiotics

To prepare the tubes for spectrophotometric analysis

To inhibit the growth of microorganisms (correct)

To enhance the growth of microorganisms

What is the main reason for preparing five test levels of the standard and a single test level of up to 20 samples in the turbidimetric assay?

To minimize the use of antibiotics

To ensure the accuracy and reproducibility of the assay (correct)

To reduce the time required for the assay

To simplify the data analysis process

In the context of bioassay of antibiotics, what is the role of including control tubes containing no antibiotic?

To assess the potential toxicity of the antibiotic

To maintain sterility during the assay

To provide a source of additional inoculum

To serve as a reference for the inhibitory effect of the antibiotic (correct)

What is the purpose of using sterile glass beads or a bent glass rod during the harvesting of organisms in the bioassay of antibiotics?

To facilitate the transfer of organisms into the harvest suspension Signup and view all the answers

Why is it necessary to prepare stock solutions of the standard and each sample in the bioassay of antibiotics?

To simplify the dilution process for the test levels Signup and view all the answers

What is the primary objective of performing a turbidimetric assay in the bioassay of antibiotics?

To determine the potency of the antibiotic Signup and view all the answers

What is the main purpose of using triplicate test levels in the bioassay of antibiotics?

To improve the accuracy of the assay Signup and view all the answers

What is the primary role of the inoculum in the bioassay of antibiotics?

To provide a source of microorganisms for growth Signup and view all the answers

Why is it important to maintain a water bath or incubator at a specific temperature during the bioassay of antibiotics?

To promote the growth of microorganisms Signup and view all the answers

What is the primary reason for using a cylinder-plate assay in the bioassay of antibiotics?