10 Questions
What is the primary purpose of adding dilute formaldehyde to each tube after incubation in the bioassay of antibiotics?
To inhibit the growth of microorganisms
What is the main reason for preparing five test levels of the standard and a single test level of up to 20 samples in the turbidimetric assay?
To ensure the accuracy and reproducibility of the assay
In the context of bioassay of antibiotics, what is the role of including control tubes containing no antibiotic?
To serve as a reference for the inhibitory effect of the antibiotic
What is the purpose of using sterile glass beads or a bent glass rod during the harvesting of organisms in the bioassay of antibiotics?
To facilitate the transfer of organisms into the harvest suspension
Why is it necessary to prepare stock solutions of the standard and each sample in the bioassay of antibiotics?
To simplify the dilution process for the test levels
What is the primary objective of performing a turbidimetric assay in the bioassay of antibiotics?
To determine the potency of the antibiotic
What is the main purpose of using triplicate test levels in the bioassay of antibiotics?
To improve the accuracy of the assay
What is the primary role of the inoculum in the bioassay of antibiotics?
To provide a source of microorganisms for growth
Why is it important to maintain a water bath or incubator at a specific temperature during the bioassay of antibiotics?
To promote the growth of microorganisms
What is the primary reason for using a cylinder-plate assay in the bioassay of antibiotics?
To determine the potency of the antibiotic
Learn about the injection procedure for sample dilutions in the bioassay of digitalis using the Pigeon Method. Understand the importance of using a small-bore buret calibrated to 0.05 mL and ensuring the absence of air bubbles in the injection apparatus.
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