Questions and Answers
What are photons absorbed by?
Pigments in chloroplast of plants
In photosynthesis, light energy is transformed into what?
Chemical energy
The chemical energy is used to synthesize organic compounds (glucose) from carbon dioxide.
True
You can determine photosynthetic activity by testing for the production of starch using I2KI.
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I2KI stains green when in the presence of starch.
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A change from the yellow amber color of iodine solution to a purple solution is a positive test for the presence of starch.
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What is the equation for photosynthesis in plants?
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What is the equation for respiration in plants, animals, and other organisms?
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Visible light cannot be reflected, transmitted, or absorbed.
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In photosynthesis, carbon dioxide and water are used to synthesize glucose and release water.
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The energy in organic molecules is released to form ATP during cellular respiration in plants, animals, and other organisms.
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The plastic filters used in the wavelengths of light for the photosynthesis exercise were designed to reflect and transmit the appropriate wavelengths of light to correspond to the visible light spectrum.
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After staining the leaf, what happened under the black filter?
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After staining the leaf, what happened under the red filter?
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There was no photosynthetic activity going on under the red filter.
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After staining the leaf, what happened under the blue filter?
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After staining the leaf, what happened under the green filter?
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Which treatment allowed the greatest photosynthetic activity in the wavelength experiment?
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When the red filter is placed on a leaf, what wavelengths of light pass through and reach the leaf cells below?
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Was starch present under the black construction paper?
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What is a pigment?
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If a pigment absorbed all wavelengths of visible light, it appears white.
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On the paper chromatography, what was the order of beta carotene, chlorophyll a and b, and xanthophyll?
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Green colors in plants are produced by?
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Yellow, orange, and bright red colors are produced by?
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What does anthocyanins usually produce?
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Why was a blender used in the paper chromatography exercise?
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What is the leading edge of the solvent called in chromatography?
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Polar molecules or substances dissolve (or are attracted to) polar molecules.
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Nonpolar molecules are repelled by nonpolar molecules to varying degrees.
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Chromatography paper (cellulose) is a polar (charged) substance.
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The solvent, made of petroleum ether and acetone, is relatively polar.
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The most nonpolar substance will dissolve in the nonpolar solvent first or last?
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The most polar substance will be attracted to the polar chromatography paper and will move?
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How can you determine polarity?
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How many oxygens are in chlorophyll b?
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How many oxygens are in chlorophyll a?
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How many oxygens are in beta carotene?
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How many oxygens are in xanthophyll?
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What is the absorption spectrum?
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What can be used to determine the absorption spectrum?
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What is a spectrophotometer?
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How does a spectrophotometer work?
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Chlorophyll b and carotenoids are called?
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A green pigment absorbs green wavelengths of visible light.
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A spectrophotometer will be used to separate plant pigments based on differences in polarity.
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Photosynthetic activity will be assessed by testing for the production of?
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The pigments that absorb photons of light are located in which part of the plant cell?
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Chlorophyll A is a green pigment that absorbs green light.
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The white pigments of the coleus leaf were not photosynthetic.
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In the chromatography experiment, the least polar pigment migrated the furthest up the chromatography paper.
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Which filter would you expect to produce the lowest photosynthetic rate in the underlying leaf?
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Which filter would you expect to produce the highest photosynthetic rate in the underlying leaf?
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What is not an input required for photosynthesis?
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What are the inputs for photosynthesis?
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What are the inputs for respiration?
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What is chromatography?
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What is the chromatography mobile phase?
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What is the chromatography stationary phase?
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Chlorophyll A is a main pigment.
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Carotenoids include?
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Study Notes
Photosynthesis Overview
- Photosynthesis involves the absorption of photons by pigments located in the chloroplasts of plants.
- Light energy is converted into chemical energy, specifically to synthesize glucose from carbon dioxide.
- The general equation for photosynthesis is: Energy + 6CO2 + 6H2O → C6H12O6 + 6O2.
Starch Production and Testing
- Photosynthetic activity can be determined by testing for starch using iodine solution (I2KI).
- A purple solution from iodine indicates the presence of starch, while no color change signifies its absence.
Light Filters and Photosynthetic Activity
- Different filters affect photosynthetic activity; red and blue filters promote the highest rates, while green filters minimize activity.
- Under a black filter, all light wavelengths are absorbed, preventing photosynthesis; under a red filter, some activity occurs due to red light transmission.
- Blue light can also stimulate some photosynthesis, but green light does not promote the process as it is not absorbed effectively.
Pigments in Plants
- Pigments include chlorophyll a and b, carotenoids, and anthocyanins, each providing unique coloration and functions.
- Chlorophyll a is the main pigment, while chlorophyll b and carotenoids assist in capturing light energy.
- Carotenoids produce yellow, orange, and red colors, whereas anthocyanins give blues, purples, and reds.
Chromatography Techniques
- Chromatography is utilized to separate plant pigments based on polarity; cellulose paper (polar) and a nonpolar solvent (petroleum ether and acetone) are used.
- The order of pigments separated by chromatography is chlorophyll b (most polar), chlorophyll a, xanthophyll, and beta-carotene (most nonpolar).
Measuring Absorption and Polarity
- A spectrophotometer measures light absorption and transmission by pigments, determining their absorption spectrum.
- The polarity of substances can be assessed by counting the number of polar oxygen atoms; the more polar a pigment, the less it travels on chromatography paper.
Inputs and Outputs
- Inputs for photosynthesis include energy (light), carbon dioxide, and water; outputs are glucose and oxygen.
- In cellular respiration, the process reverses: glucose and oxygen are converted into energy, carbon dioxide, and water.
General Facts
- Chlorophyll a specifically does not absorb green light, instead reflecting it, which is why plants appear green.
- Nonpolar solvents are most effective at dissolving nonpolar substances, with the least polar moving the furthest in chromatography.
Key Definitions
- Accessory pigments: chlorophyll b and carotenoids that aid in photosynthesis.
- Absorption spectrum: the pattern of light absorption for specific pigments at varying wavelengths.
- Chromatography: a laboratory technique to separate mixtures based on varying solubilities and polarities.
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Description
Test your knowledge on the process of photosynthesis with this engaging quiz. Learn about the role of photons, chloroplasts, and the conversion of light energy into chemical energy. Perfect for biology students and enthusiasts alike!