BIO 184 Recombinant DNA Technology Lab

Questions and Answers

What is the function of a micropipette?

To measure and deliver small volumes of liquid

What volumes can a micropipette measure?

From 1 μL to 1 mL

What are the qualities that make a good vector in recombinant DNA technology?

Contains a selectable marker (correct)

Ability to replicate independently of the host (correct)

Low cloning capacity

High mutation rate

Which of the following represents the volume range of a p20 micropipette?

1-20 μL Signup and view all the answers

Restriction enzymes are used for creating recombinant DNA molecules.

True Signup and view all the answers

What is one practical application for recombinant DNA technology?

All of the above Signup and view all the answers

Why are scientists cloning a portion of a bacterial ribosomal gene?

To study urban outflow waters entering the Sacramento River Signup and view all the answers

What is a microcentrifuge used for?

Rapid separation of small quantities of cells and large molecules Signup and view all the answers

1L (liter) = 103 mL (milliliter) = _____ µL (microliter)

106 Signup and view all the answers

Study Notes

Experiment Overview

Introduces recombinant DNA technology and equipment used in molecular genetics.
Focus on the assembly of recombinant DNA molecules as part of the River Science Project.

Objectives

Master the use of micropipettes for volumes from 1 μL to 1 mL.
Operate microcentrifuges and vortexers safely.
Define "vector" and identify characteristics of effective vectors.
Describe the role of restriction enzymes in creating recombinant DNA.
Explain ligation techniques for incorporating foreign DNA into vectors.
Recognize practical applications of recombinant DNA technology.
Understand the purpose of cloning a bacterial ribosomal gene from urban outflow points in the Sacramento River.

Equipment Essentials

Molecular biology requires specialized equipment due to small volume handling.
Micropipettes are used for precise liquid measurements, with a capability of down to 1 μL.
Microcentrifuge tubes generally hold 0.2-2.0 mL for various reactions.
Vortexers enable quick mixing of small volumes while microcentrifuges facilitate rapid separation of substances.

Volume Conversions

1 Liter (L) equals 1,000 Milliliters (mL) and equals 1,000,000 Microliters (μL).

Micropipette Volume Ranges

p20: measures 1-20 μL
p200: measures 20-200 μL
p1000: measures 200-1000 μL

Overview of Recombinant DNA Technology

Not specified in detail in the provided content. Further information would elaborate on the techniques and methodology in recombinant DNA creation.

Description

This quiz focuses on the introductory principles of recombinant DNA technology outlined in the BIO 184 laboratory manual at California State University, Sacramento. You will explore the basic equipment used in molecular genetics, such as micropipettes and microcentrifuges, as well as participate in the River Science Project. Prepare to engage with fundamental concepts and practices in laboratory settings.