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Questions and Answers
What is binary fission?
What is binary fission?
The division of a bacterial cell.
The steps of binary fission include the parent cell enlarges and duplicates _____
The steps of binary fission include the parent cell enlarges and duplicates _____
DNA
In binary fission, septum formation divides the cell into two _____ chambers.
In binary fission, septum formation divides the cell into two _____ chambers.
separate
What is generation time?
What is generation time?
What phases are included in the growth curve?
What phases are included in the growth curve?
What occurs during the lag phase?
What occurs during the lag phase?
What characterizes the log phase?
What characterizes the log phase?
What happens during the stationary phase?
What happens during the stationary phase?
What is the death phase?
What is the death phase?
What methods are used for the enumeration of bacteria?
What methods are used for the enumeration of bacteria?
What does turbidity refer to?
What does turbidity refer to?
What is direct cell count?
What is direct cell count?
What is an automated device/cell counter?
What is an automated device/cell counter?
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Study Notes
Binary Fission
- A method of asexual reproduction in bacteria involving cell division.
Steps of Binary Fission
- Parent cell enlarges, DNA is duplicated.
- Septum formation occurs, dividing the cell into two chambers.
- Cell division results in two genetically identical daughter cells.
Generation Time
- Refers to the duration needed for one complete division cycle.
- Indicates the growth rate of bacteria, measured exponentially to outline bacterial population increase.
Growth Curve Phases
- Lag Phase: Cells adapt, grow, and synthesize proteins; no maximum growth occurs.
- Log Phase: Rapid exponential growth under optimal nutrient and environmental conditions.
- Stationary Phase: Nutrient depletion and waste accumulation stabilize cell numbers; growth rate equals death rate.
- Death Phase: High cell mortality due to nutrient exhaustion; experiments can use a chemostat to maintain nutrient levels and prevent this phase.
Enumeration of Bacteria
- Methods to quantify bacterial populations:
- Turbidity: Cloudiness visible to the eye, indicating bacterial presence.
- Direct Cell Count: Total of live and dead cells counted using a special slide with a measured grid.
- Automated Devices/Cell Counters: Utilize electronic sensors for accurate cell counting.
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