Questions and Answers
What is one of the main applications of spectrophotometry in various fields?
Quantitative analysis
Name two specific fields where spectrophotometry finds significant application.
Chemistry and biochemistry
What is one of the primary uses of spectrophotometry in the food industry?
Quality control
Give an example of a biochemical sample that can be analyzed using spectrophotometry.
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What is the primary goal of spectrophotometric analysis in clinical applications?
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List two other examples of biochemical samples that can be analyzed using spectrophotometry.
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Name one specific type of biochemical analysis that spectrophotometry can be used for.
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What is the main advantage of using spectrophotometry for quantitative analysis?
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What are some of the fields where spectrophotometry is used in addition to the ones mentioned?
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What is the principle behind spectrophotometry?
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Study Notes
Absorbance and Sample Preparation
- Absorbance of a sample is directly proportional to the sample's thickness and concentration.
- A sample (test) is prepared from the biological fluid being analyzed and undergoes all steps of analysis to determine the concentration of an analyte.
Role of Solutions in Spectrophotometry
- Known concentration solutions are used to compare with the unknown concentration of the same substance in the sample.
Applications of Spectrophotometry
- Estimation of biochemical samples, such as:
- Plasma
- Serum
- Urine
- Used in the food industry
- Quantitative analysis in various fields, including:
- Chemistry
- Physics
- Biochemistry
- Forensic science
- Agriculture
- Material and chemical engineering
- Clinical applications
- Specific clinical applications include:
- Determining glucose levels in blood
- Measuring cholesterol levels in blood
- Analyzing blood proteins
- Determining creatinine levels in blood
- Measuring urea levels in blood
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Description
Understanding the relationship between absorbance, sample thickness, and concentration, as described by Beer's Law. Important concept in medical laboratory technology.
