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BCA Assay Quiz

Created by

@RespectablePyrope

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Questions and Answers

What is the BCA assay used for?

Determining the total concentration of protein in a solution (correct)

Determining the pH of a solution

Determining the concentration of lipids in a solution

Determining the concentration of carbohydrates in a solution

Which company was the inventor of the BCA assay associated with?

Biochemical Innovations Inc.

Thermo Fisher Scientific

Pierce Chemical Company (correct)

Smith & Smith Company

What causes the color change in the BCA assay?

Protein concentration (correct)

Carbohydrate concentration

Lipid concentration

pH level

When was the BCA assay patented by Pierce Chemical Company?

<p>1989</p> Signup and view all the answers

What pH does the stock BCA solution have?

<p>pH 11.25</p> Signup and view all the answers

Study Notes

BCA Assay Overview

The BCA (Bicinchoninic Acid) assay is a type of biochemical assay used to determine the total protein concentration in a sample.

History and Development

The BCA assay was invented by Pierce Chemical Company.

Assay Mechanism

The BCA assay is based on the reduction of Cu2+ to Cu+ by protein, which then reacts with BCA to form a purple-colored complex, causing a color change.

Patent and Development Timeline

The BCA assay was patented by Pierce Chemical Company in 1985.

Solution Properties

The stock BCA solution has a pH of 11.25.

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Description

Test your knowledge of protein quantification methods with this quiz on the Bicinchoninic Acid (BCA) assay. Explore the principles, applications, and significance of this biochemical assay in determining total protein concentration.