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Questions and Answers
What is the BCA assay used for?
What is the BCA assay used for?
- Determining the total concentration of protein in a solution (correct)
- Determining the pH of a solution
- Determining the concentration of lipids in a solution
- Determining the concentration of carbohydrates in a solution
Which company was the inventor of the BCA assay associated with?
Which company was the inventor of the BCA assay associated with?
- Biochemical Innovations Inc.
- Thermo Fisher Scientific
- Pierce Chemical Company (correct)
- Smith & Smith Company
What causes the color change in the BCA assay?
What causes the color change in the BCA assay?
- Protein concentration (correct)
- Carbohydrate concentration
- Lipid concentration
- pH level
When was the BCA assay patented by Pierce Chemical Company?
When was the BCA assay patented by Pierce Chemical Company?
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What pH does the stock BCA solution have?
What pH does the stock BCA solution have?
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Study Notes
BCA Assay Overview
- The BCA (Bicinchoninic Acid) assay is a type of biochemical assay used to determine the total protein concentration in a sample.
History and Development
- The BCA assay was invented by Pierce Chemical Company.
Assay Mechanism
- The BCA assay is based on the reduction of Cu2+ to Cu+ by protein, which then reacts with BCA to form a purple-colored complex, causing a color change.
Patent and Development Timeline
- The BCA assay was patented by Pierce Chemical Company in 1985.
Solution Properties
- The stock BCA solution has a pH of 11.25.
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