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Questions and Answers
What is the BCA assay used for?
What is the BCA assay used for?
- Determining the total concentration of protein in a solution (correct)
- Determining the pH of a solution
- Determining the concentration of lipids in a solution
- Determining the concentration of carbohydrates in a solution
Which company was the inventor of the BCA assay associated with?
Which company was the inventor of the BCA assay associated with?
- Biochemical Innovations Inc.
- Thermo Fisher Scientific
- Pierce Chemical Company (correct)
- Smith & Smith Company
What causes the color change in the BCA assay?
What causes the color change in the BCA assay?
- Protein concentration (correct)
- Carbohydrate concentration
- Lipid concentration
- pH level
When was the BCA assay patented by Pierce Chemical Company?
When was the BCA assay patented by Pierce Chemical Company?
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What pH does the stock BCA solution have?
What pH does the stock BCA solution have?
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Study Notes
BCA Assay Overview
- The BCA (Bicinchoninic Acid) assay is a type of biochemical assay used to determine the total protein concentration in a sample.
History and Development
- The BCA assay was invented by Pierce Chemical Company.
Assay Mechanism
- The BCA assay is based on the reduction of Cu2+ to Cu+ by protein, which then reacts with BCA to form a purple-colored complex, causing a color change.
Patent and Development Timeline
- The BCA assay was patented by Pierce Chemical Company in 1985.
Solution Properties
- The stock BCA solution has a pH of 11.25.
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Description
Test your knowledge of protein quantification methods with this quiz on the Bicinchoninic Acid (BCA) assay. Explore the principles, applications, and significance of this biochemical assay in determining total protein concentration.
