Basic Microbiology Practical No. 6

<p>Consider all specimens infectious (B)</p> Signup and view all the answers

<p>Lactose from non-lactose fermenters (C)</p> Signup and view all the answers

<p>To detect the presence of cytochrome c oxidase (C)</p> Signup and view all the answers

<p>Slide coagulase test (A)</p> Signup and view all the answers

<p>To ensure accurate identification of microorganisms (C)</p> Signup and view all the answers

<p>It binds to cells and forms fibrin clots. (A)</p> Signup and view all the answers

<p>Colorless to dark blue-indigo-purple (B)</p> Signup and view all the answers

<p>To split urea into ammonia and CO2. (A)</p> Signup and view all the answers

<p>Enterobacter and non-fastidious Gram-negative rods (A)</p> Signup and view all the answers

<p>Presence of a blue ring on filter paper (B)</p> Signup and view all the answers

<p>Strong urease-positive organisms (B)</p> Signup and view all the answers

<p>It helps to alkalinize the medium. (D)</p> Signup and view all the answers

<p>Cultures must be growing on solid media. (A)</p> Signup and view all the answers

<p>Combining multiple tests simultaneously (D)</p> Signup and view all the answers

<p>Light orange (D)</p> Signup and view all the answers

<p>Pink to red colour (D)</p> Signup and view all the answers

<p>Sample A (D)</p> Signup and view all the answers

<p>Klebsiella (B)</p> Signup and view all the answers

<p>The presence of motility (D)</p> Signup and view all the answers

<p>Escherichia coli (B)</p> Signup and view all the answers

<p>Gelling of the plasma (A)</p> Signup and view all the answers

<p>Pseudomonas aeruginosa (D)</p> Signup and view all the answers

<p>Dark purple color development (C)</p> Signup and view all the answers

<p>37ºC (A)</p> Signup and view all the answers

<p>Gelling indicates S.aureus (B)</p> Signup and view all the answers

<p>Delayed color changes (B)</p> Signup and view all the answers

<p>To identify urease-producing organisms (A)</p> Signup and view all the answers

<p>Absence of color change (C)</p> Signup and view all the answers

<p>Salmonella sp (D)</p> Signup and view all the answers

<p>At various intervals up to 4 hours (A)</p> Signup and view all the answers

<p>Rapid appearance of sustained gas bubbles (A)</p> Signup and view all the answers

<p>Staphylococcus aureus as positive control (C)</p> Signup and view all the answers

<p>No agglutination or clumping (B)</p> Signup and view all the answers

<p>Discard the reagent and use a fresh one (B)</p> Signup and view all the answers

<p>Conversion of fibrinogen to fibrin by thrombin (B)</p> Signup and view all the answers

<p>Streptococcus species (C)</p> Signup and view all the answers

<p>The integrity of the packaging and components (C)</p> Signup and view all the answers

<p>Clot formation in the presence of mammalian plasma (C)</p> Signup and view all the answers

<p>Adding a drop of H2O2 and observing for bubbles (D)</p> Signup and view all the answers

<p>Safety cabinet for handling samples (D)</p> Signup and view all the answers

An enzyme that breaks down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2).

A test to determine the presence of the catalase enzyme in bacteria.

An enzyme produced by Staphylococcus aureus that clots blood plasma.

A test to detect the presence of coagulase in bacteria, aiding in identification.

Growth media that promote the growth of specific types of bacteria.

Media allowing for the differentiation of bacteria based on metabolic properties.

Bacteria able to ferment lactose, often producing acidic byproducts, visible on certain media.

Bacteria unable to ferment lactose.

The bound form of coagulase that cross-links fibrinogen causing bacterial clumping.

A test used to determine if bacteria produce cytochrome c oxidase.

An enzyme that reduces a reagent, causing a color change.

A test to see if bacteria can break down urea into ammonia and carbon dioxide.

A medium used to differentiate enteric bacteria based on their ability to produce urease.

A multi-test system for identifying bacteria, including enteric bacteria

Tests that identify bacteria based on their metabolic activities.

Organisms that turn the entire medium pink within 24 hours in a urease test

A group of bacteria that do not need special growth conditions and are Gram negative with a rod shape

A type of bacteria that stains pink or red with Gram staining, has a thin cell wall, and is rod-shaped.

The ability of bacteria to move independently, often using flagella.

The process where bacteria break down lactose sugar, often producing acidic byproducts.

A selective and differential media used to identify lactose fermenting bacteria based on colony color.

Detects bacteria that produce the enzyme catalase, which breaks down hydrogen peroxide (H₂O₂).

Rapid appearance of sustained gas bubbles when bacterial colony is exposed to H2O2.

No gas bubbles are produced upon exposure to H2O2.

Detects bacteria that produce coagulase, an enzyme that causes blood plasma clotting.

A rapid method to detect coagulase in a bacterial sample by observing the clumping or agglutination of cocci on slide.

Clumping or agglutination of cocci within 5-10 seconds after mixing bacterial sample with plasma.

No clumping or agglutination observed within 5-10 seconds.

Detects the presence of free coagulase by observing whether bacterial sample causes plasma to clot.

Bacterial sample causes clotting (coagulation) of plasma (serum).

Bacterial sample does not cause plasma (serum) to clot.

The plasma in the tube turns into a gel (clot) indicating the presence of coagulase, likely Staphylococcus aureus.

The plasma remains liquid, suggesting the absence of coagulase, hinting at the possibility of Staphylococcus epidermidis.

One method of performing the oxidase test where a drop of oxidase reagent is put on a filter paper and then the organism is touched to it.

Rapid development of dark purple color within 10 seconds on the filter paper, indicating the presence of the oxidase enzyme.

Absence of color change on the filter paper, indicating the absence of the oxidase enzyme.

A specialized media used in the urease test that contains urea and a pH indicator.

The medium turns pink or red due to the production of ammonia by the bacterium, indicating a positive result.