Podcast
Questions and Answers
What is the correct statement about hand washing?
What is the correct statement about hand washing?
- Hand washing is not necessary if gloves are worn.
- Hand washing is only required if there are visible signs of dirt on hands.
- Hand washing is essential before wearing gloves. (correct)
- Hand washing is important after using gloves.
What should be done if there are signs of tear in gloves during laboratory work?
What should be done if there are signs of tear in gloves during laboratory work?
- Still use gloves even when there are signs of tear.
- Take off the gloves and continue working without them.
- Continue working without replacing the gloves.
- Stop working immediately and replace the torn gloves. (correct)
What is the purpose of using Cotton Plugs?
What is the purpose of using Cotton Plugs?
- To indicate the level of sterility in the lab.
- To seal containers for long-term storage of samples.
- To clean laboratory equipment.
- To prevent contamination in a sterile field. (correct)
Why is it important to sanitize the working area before and after use?
Why is it important to sanitize the working area before and after use?
What is an autoclave used for in a laboratory setting?
What is an autoclave used for in a laboratory setting?
When should transport media be used in laboratory settings?
When should transport media be used in laboratory settings?
Which statement regarding sterile items and the sterile field is TRUE?
Which statement regarding sterile items and the sterile field is TRUE?
What is the correct procedure regarding gloves during laboratory work?
What is the correct procedure regarding gloves during laboratory work?
What is the appropriate temperature for sterilization in an autoclave?
What is the appropriate temperature for sterilization in an autoclave?
Which type of media is used for storing bacteria for a long period of time?
Which type of media is used for storing bacteria for a long period of time?
What is the main purpose of using Cotton Plugs in laboratory equipment?
What is the main purpose of using Cotton Plugs in laboratory equipment?
Why is it necessary to sanitize the working area after use in a laboratory setting?
Why is it necessary to sanitize the working area after use in a laboratory setting?
When should transport media typically be used in laboratory settings?
When should transport media typically be used in laboratory settings?
What is the main function of an autoclave in laboratory procedures?
What is the main function of an autoclave in laboratory procedures?
Which statement accurately describes hand washing requirements in a laboratory setting?
Which statement accurately describes hand washing requirements in a laboratory setting?
Study Notes
Sterility and Contamination
- An item is either sterile or not sterile, with no in-between states.
- Only sterile items can be placed on the sterile field.
- Hand washing is still necessary even when wearing gloves.
- Gloves should be replaced immediately if they show signs of tears or damage.
- The working area should be sanitized before and after use to prevent contamination.
Media Types
- Indicator (Differential) Media: used to detect specific organisms, causing a change in the indicator (e.g., Blood agar and MacConkey agar).
- Transport Media: used when a specimen cannot be cultured soon after collection (e.g., Cary-Blair medium, Stuart medium).
- Storage Media: used for storing bacteria for a long period of time (e.g., Egg saline medium, chalk cooked meat broth).
Devices and Equipment
- Autoclave: used to sterilize and disinfect objects at 121°C for 15 minutes, with a cooling down temperature of 50°C.
- Membrane filtration vacuum pump: used for sterilization and filtration processes.
- Refrigerator: used for maintaining and storing stock cultures between subculturing periods and storing media to prevent dehydration.
- Biosafety Cabinets (BSC): used for aseptic techniques and handling of infectious materials.
- Cultivation Chambers: used for growing and culturing microorganisms.
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Description
Test your knowledge on basic laboratory techniques with this quiz. Includes questions about sterile items, hand washing, use of gloves, and sanitization of working areas.
