Basic Laboratory Techniques Quiz

Questions and Answers

What is the correct statement about hand washing?

Hand washing is essential before wearing gloves.

What should be done if there are signs of tear in gloves during laboratory work?

Stop working immediately and replace the torn gloves.

What is the purpose of using Cotton Plugs?

To prevent contamination in a sterile field.

Why is it important to sanitize the working area before and after use?

To reduce the risk of contamination and maintain aseptic conditions. Signup and view all the answers

What is an autoclave used for in a laboratory setting?

To sterilize and disinfect objects for infection control. Signup and view all the answers

When should transport media be used in laboratory settings?

When specimen cannot be cultured soon after collection. Signup and view all the answers

Which statement regarding sterile items and the sterile field is TRUE?

Only sterile items can be placed on the sterile field. Signup and view all the answers

What is the correct procedure regarding gloves during laboratory work?

Gloves should still be used even when there are signs of tear. Signup and view all the answers

What is the appropriate temperature for sterilization in an autoclave?

121°C for 15 minutes Signup and view all the answers

Which type of media is used for storing bacteria for a long period of time?

Storage Media Signup and view all the answers

What is the main purpose of using Cotton Plugs in laboratory equipment?

To prevent contamination during storage Signup and view all the answers

Why is it necessary to sanitize the working area after use in a laboratory setting?

To prevent the spread of infectious diseases and maintain hygiene. Signup and view all the answers

When should transport media typically be used in laboratory settings?

When specimen culture cannot be done soon after collection. Signup and view all the answers

What is the main function of an autoclave in laboratory procedures?

To sterilize and disinfect objects using high-pressure steam. Signup and view all the answers

Which statement accurately describes hand washing requirements in a laboratory setting?

Hand washing should be done even when gloves are worn. Signup and view all the answers

Study Notes

Sterility and Contamination

An item is either sterile or not sterile, with no in-between states.
Only sterile items can be placed on the sterile field.
Hand washing is still necessary even when wearing gloves.
Gloves should be replaced immediately if they show signs of tears or damage.
The working area should be sanitized before and after use to prevent contamination.

Media Types

Indicator (Differential) Media: used to detect specific organisms, causing a change in the indicator (e.g., Blood agar and MacConkey agar).
Transport Media: used when a specimen cannot be cultured soon after collection (e.g., Cary-Blair medium, Stuart medium).
Storage Media: used for storing bacteria for a long period of time (e.g., Egg saline medium, chalk cooked meat broth).

Devices and Equipment

Autoclave: used to sterilize and disinfect objects at 121°C for 15 minutes, with a cooling down temperature of 50°C.
Membrane filtration vacuum pump: used for sterilization and filtration processes.
Refrigerator: used for maintaining and storing stock cultures between subculturing periods and storing media to prevent dehydration.
Biosafety Cabinets (BSC): used for aseptic techniques and handling of infectious materials.
Cultivation Chambers: used for growing and culturing microorganisms.