Bacteriology: Escherichia coli and Campylobacter
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Questions and Answers

What is the characteristic of Escherichia coli?

  • Motile and obligate aerobe
  • Gram-positive bacilli
  • Non-motile and obligate anaerobe
  • Gram-negative bacilli (correct)
  • Campylobacter spp. are able to use sugars either oxidatively or fermentatively.

    False

    What is the primary characteristic of Pseudomonas?

    Strictly aerobic

    Acinetobacter are characterized by being ______________.

    <p>non-motile</p> Signup and view all the answers

    Match the following bacteria with their characteristics:

    <p>Escherichia coli = Gram-negative bacillus Campylobacter spp. = Microaerophilic and capnophilic Pseudomonas = Strictly aerobic Acinetobacter = Non-motile and capsulated</p> Signup and view all the answers

    What is the main characteristic of Chryseobacterium?

    <p>Gram-negative rods</p> Signup and view all the answers

    Proteus mirabilis is a Gram-positive bacillus.

    <p>False</p> Signup and view all the answers

    What is the primary habitat of Proteus mirabilis?

    <p>Soil and water and part of the normal human intestinal flora</p> Signup and view all the answers

    What is the characteristic of Haemophilus?

    <p>Gram-negative</p> Signup and view all the answers

    Listeria spp. are obligate anaerobes.

    <p>False</p> Signup and view all the answers

    What is the optimal growth temperature for Listeria monocytogenes?

    <p>35-37°C</p> Signup and view all the answers

    Clostridium species are characterized by forming ______________________.

    <p>endospores</p> Signup and view all the answers

    Match the following bacteria with their characteristics:

    <p>Haemophilus = Non-lactose fermenter and oxidase -ve Listeria = Motile, facultatively anaerobic Clostridium = Spore former, obligate anaerobe</p> Signup and view all the answers

    Which of the following is NOT a characteristic of Haemophilus?

    <p>Motile</p> Signup and view all the answers

    Clostridium species are oxygen tolerant.

    <p>False</p> Signup and view all the answers

    Haemophilus Type B vaccine is abbreviated as ______________________.

    <p>HIB</p> Signup and view all the answers

    What is the characteristic of Escherichia coli in terms of its ability to grow in different environments?

    <p>Facultative anaerobe</p> Signup and view all the answers

    Campylobacter spp. are able to grow in the presence of normal oxygen levels.

    <p>False</p> Signup and view all the answers

    What is the primary source of energy for Campylobacter spp.?

    <p>Amino acids</p> Signup and view all the answers

    Pseudomonas is characterized by being ______________, with one or more polar flagella.

    <p>motile</p> Signup and view all the answers

    Match the following bacteria with their characteristics:

    <p>Acinetobacter = Short bacilli in pairs Chryseobacterium = Yellow pigment on BA Pseudomonas = Motile by 1 or more polar flagella Proteus mirabilis = Highly motile and non-spore-forming</p> Signup and view all the answers

    Chryseobacterium is a Gram-positive rod.

    <p>False</p> Signup and view all the answers

    What is the primary habitat of Proteus mirabilis?

    <p>Soil and water</p> Signup and view all the answers

    What is the primary disease caused by Proteus mirabilis?

    <p>Urinary tract infections (UTIs)</p> Signup and view all the answers

    What is the shape of Haemophilus bacteria?

    <p>Pleomorphic small bacilli</p> Signup and view all the answers

    Listeria spp. are obligate anaerobes.

    <p>False</p> Signup and view all the answers

    What is the optimal growth temperature for Listeria monocytogenes?

    <p>35°C to 37°C</p> Signup and view all the answers

    Clostridium species are characterized by forming _______________.

    <p>Endospores</p> Signup and view all the answers

    Which of the following is a characteristic of Listeria monocytogenes?

    <p>Gram-positive</p> Signup and view all the answers

    Match the following bacteria with their characteristics:

    <p>Haemophilus = Facultative anaerobe Listeria = Gram-positive Clostridium = Spore former</p> Signup and view all the answers

    Clostridium species are oxygen tolerant.

    <p>False</p> Signup and view all the answers

    What is the characteristic of Haemophilus that makes it super virulent?

    <p>Capsulated</p> Signup and view all the answers

    Study Notes

    Escherichia coli (E. coli)

    • Gram-negative bacillus
    • Motile and facultative anaerobe
    • Non-lactose fermenter and urease-negative
    • Oxidase and H2S-negative

    Campylobacter spp.

    • Gram-negative, slender, curved, or spiral-shaped rods
    • Motile with single polar unsheathed flagellum
    • Microaerophilic and capnophilic
    • Grow at 42°C, adapted to chickens with higher body temperature
    • Unable to use sugars, derive energy from amino acids
    • Virulence factors: motility and heat-labile enterotoxin

    Pseudomonas spp.

    • Gram-negative rods
    • Motile with polar flagella
    • Non-spore former
    • Strictly aerobic, oxidase-positive, and catalase-positive

    Acinetobacter spp.

    • Gram-negative short bacilli in pairs
    • Non-motile, capsulated, and strictly aerobic
    • Oxidase-negative and catalase-positive

    Chryseobacterium spp.

    • Gram-negative rods
    • Non-motile, yellow pigment on blood agar (BA)
    • Strictly aerobic, oxidase-positive, and catalase-positive

    Proteus mirabilis

    • Gram-negative bacillus, highly motile
    • Facultative anaerobic, non-lactose fermenter, and oxidase-negative
    • Urease-positive, causes urinary tract infections (UTIs)

    Haemophilus spp.

    • Gram-negative, pleomorphic small bacilli
    • Non-motile, non-spore former, and encapsulated
    • Aerobic, facultatively anaerobic, oxidase-positive, and catalase-variable
    • Capsulated forms are Type B, highly virulent, and prevented by HIB vaccine

    Listeria spp.

    • Gram-positive coccobacilli, motile at room temperature
    • Non-spore former, facultatively anaerobic, and intra-cellular
    • Oxidase-negative, catalase-positive, and β-haemolytic
    • Grow optimally at 35-37°C, but also at 4°C
    • Associated with unwashed lettuce, deli meats, and vulnerable individuals (e.g., pregnant women)

    Clostridium spp.

    • Gram-positive, straight or curved rods, forming chains
    • Spore former, motile with peritrichous flagella
    • Obligate anaerobes, saccharolytic, and proteolytic
    • Catalase-negative, with some species oxygen-tolerant or dying in oxygen

    Escherichia coli (E. coli)

    • Gram-negative bacillus
    • Motile and facultative anaerobe
    • Non-lactose fermenter and urease-negative
    • Oxidase and H2S-negative

    Campylobacter spp.

    • Gram-negative, slender, curved, or spiral-shaped rods
    • Motile with single polar unsheathed flagellum
    • Microaerophilic and capnophilic
    • Grow at 42°C, adapted to chickens with higher body temperature
    • Unable to use sugars, derive energy from amino acids
    • Virulence factors: motility and heat-labile enterotoxin

    Pseudomonas spp.

    • Gram-negative rods
    • Motile with polar flagella
    • Non-spore former
    • Strictly aerobic, oxidase-positive, and catalase-positive

    Acinetobacter spp.

    • Gram-negative short bacilli in pairs
    • Non-motile, capsulated, and strictly aerobic
    • Oxidase-negative and catalase-positive

    Chryseobacterium spp.

    • Gram-negative rods
    • Non-motile, yellow pigment on blood agar (BA)
    • Strictly aerobic, oxidase-positive, and catalase-positive

    Proteus mirabilis

    • Gram-negative bacillus, highly motile
    • Facultative anaerobic, non-lactose fermenter, and oxidase-negative
    • Urease-positive, causes urinary tract infections (UTIs)

    Haemophilus spp.

    • Gram-negative, pleomorphic small bacilli
    • Non-motile, non-spore former, and encapsulated
    • Aerobic, facultatively anaerobic, oxidase-positive, and catalase-variable
    • Capsulated forms are Type B, highly virulent, and prevented by HIB vaccine

    Listeria spp.

    • Gram-positive coccobacilli, motile at room temperature
    • Non-spore former, facultatively anaerobic, and intra-cellular
    • Oxidase-negative, catalase-positive, and β-haemolytic
    • Grow optimally at 35-37°C, but also at 4°C
    • Associated with unwashed lettuce, deli meats, and vulnerable individuals (e.g., pregnant women)

    Clostridium spp.

    • Gram-positive, straight or curved rods, forming chains
    • Spore former, motile with peritrichous flagella
    • Obligate anaerobes, saccharolytic, and proteolytic
    • Catalase-negative, with some species oxygen-tolerant or dying in oxygen

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