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Bacteriology: Escherichia coli and Campylobacter

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32 Questions

What is the characteristic of Escherichia coli?

Gram-negative bacilli

Campylobacter spp. are able to use sugars either oxidatively or fermentatively.

False

What is the primary characteristic of Pseudomonas?

Strictly aerobic

Acinetobacter are characterized by being ______________.

non-motile

Match the following bacteria with their characteristics:

Escherichia coli = Gram-negative bacillus Campylobacter spp. = Microaerophilic and capnophilic Pseudomonas = Strictly aerobic Acinetobacter = Non-motile and capsulated

What is the main characteristic of Chryseobacterium?

Gram-negative rods

Proteus mirabilis is a Gram-positive bacillus.

False

What is the primary habitat of Proteus mirabilis?

Soil and water and part of the normal human intestinal flora

What is the characteristic of Haemophilus?

Gram-negative

Listeria spp. are obligate anaerobes.

False

What is the optimal growth temperature for Listeria monocytogenes?

35-37°C

Clostridium species are characterized by forming ______________________.

endospores

Match the following bacteria with their characteristics:

Haemophilus = Non-lactose fermenter and oxidase -ve Listeria = Motile, facultatively anaerobic Clostridium = Spore former, obligate anaerobe

Which of the following is NOT a characteristic of Haemophilus?

Motile

Clostridium species are oxygen tolerant.

False

Haemophilus Type B vaccine is abbreviated as ______________________.

HIB

What is the characteristic of Escherichia coli in terms of its ability to grow in different environments?

Facultative anaerobe

Campylobacter spp. are able to grow in the presence of normal oxygen levels.

False

What is the primary source of energy for Campylobacter spp.?

Amino acids

Pseudomonas is characterized by being ______________, with one or more polar flagella.

motile

Match the following bacteria with their characteristics:

Acinetobacter = Short bacilli in pairs Chryseobacterium = Yellow pigment on BA Pseudomonas = Motile by 1 or more polar flagella Proteus mirabilis = Highly motile and non-spore-forming

Chryseobacterium is a Gram-positive rod.

False

What is the primary habitat of Proteus mirabilis?

Soil and water

What is the primary disease caused by Proteus mirabilis?

Urinary tract infections (UTIs)

What is the shape of Haemophilus bacteria?

Pleomorphic small bacilli

Listeria spp. are obligate anaerobes.

False

What is the optimal growth temperature for Listeria monocytogenes?

35°C to 37°C

Clostridium species are characterized by forming _______________.

Endospores

Which of the following is a characteristic of Listeria monocytogenes?

Gram-positive

Match the following bacteria with their characteristics:

Haemophilus = Facultative anaerobe Listeria = Gram-positive Clostridium = Spore former

Clostridium species are oxygen tolerant.

False

What is the characteristic of Haemophilus that makes it super virulent?

Capsulated

Study Notes

Escherichia coli (E. coli)

  • Gram-negative bacillus
  • Motile and facultative anaerobe
  • Non-lactose fermenter and urease-negative
  • Oxidase and H2S-negative

Campylobacter spp.

  • Gram-negative, slender, curved, or spiral-shaped rods
  • Motile with single polar unsheathed flagellum
  • Microaerophilic and capnophilic
  • Grow at 42°C, adapted to chickens with higher body temperature
  • Unable to use sugars, derive energy from amino acids
  • Virulence factors: motility and heat-labile enterotoxin

Pseudomonas spp.

  • Gram-negative rods
  • Motile with polar flagella
  • Non-spore former
  • Strictly aerobic, oxidase-positive, and catalase-positive

Acinetobacter spp.

  • Gram-negative short bacilli in pairs
  • Non-motile, capsulated, and strictly aerobic
  • Oxidase-negative and catalase-positive

Chryseobacterium spp.

  • Gram-negative rods
  • Non-motile, yellow pigment on blood agar (BA)
  • Strictly aerobic, oxidase-positive, and catalase-positive

Proteus mirabilis

  • Gram-negative bacillus, highly motile
  • Facultative anaerobic, non-lactose fermenter, and oxidase-negative
  • Urease-positive, causes urinary tract infections (UTIs)

Haemophilus spp.

  • Gram-negative, pleomorphic small bacilli
  • Non-motile, non-spore former, and encapsulated
  • Aerobic, facultatively anaerobic, oxidase-positive, and catalase-variable
  • Capsulated forms are Type B, highly virulent, and prevented by HIB vaccine

Listeria spp.

  • Gram-positive coccobacilli, motile at room temperature
  • Non-spore former, facultatively anaerobic, and intra-cellular
  • Oxidase-negative, catalase-positive, and β-haemolytic
  • Grow optimally at 35-37°C, but also at 4°C
  • Associated with unwashed lettuce, deli meats, and vulnerable individuals (e.g., pregnant women)

Clostridium spp.

  • Gram-positive, straight or curved rods, forming chains
  • Spore former, motile with peritrichous flagella
  • Obligate anaerobes, saccharolytic, and proteolytic
  • Catalase-negative, with some species oxygen-tolerant or dying in oxygen

Escherichia coli (E. coli)

  • Gram-negative bacillus
  • Motile and facultative anaerobe
  • Non-lactose fermenter and urease-negative
  • Oxidase and H2S-negative

Campylobacter spp.

  • Gram-negative, slender, curved, or spiral-shaped rods
  • Motile with single polar unsheathed flagellum
  • Microaerophilic and capnophilic
  • Grow at 42°C, adapted to chickens with higher body temperature
  • Unable to use sugars, derive energy from amino acids
  • Virulence factors: motility and heat-labile enterotoxin

Pseudomonas spp.

  • Gram-negative rods
  • Motile with polar flagella
  • Non-spore former
  • Strictly aerobic, oxidase-positive, and catalase-positive

Acinetobacter spp.

  • Gram-negative short bacilli in pairs
  • Non-motile, capsulated, and strictly aerobic
  • Oxidase-negative and catalase-positive

Chryseobacterium spp.

  • Gram-negative rods
  • Non-motile, yellow pigment on blood agar (BA)
  • Strictly aerobic, oxidase-positive, and catalase-positive

Proteus mirabilis

  • Gram-negative bacillus, highly motile
  • Facultative anaerobic, non-lactose fermenter, and oxidase-negative
  • Urease-positive, causes urinary tract infections (UTIs)

Haemophilus spp.

  • Gram-negative, pleomorphic small bacilli
  • Non-motile, non-spore former, and encapsulated
  • Aerobic, facultatively anaerobic, oxidase-positive, and catalase-variable
  • Capsulated forms are Type B, highly virulent, and prevented by HIB vaccine

Listeria spp.

  • Gram-positive coccobacilli, motile at room temperature
  • Non-spore former, facultatively anaerobic, and intra-cellular
  • Oxidase-negative, catalase-positive, and β-haemolytic
  • Grow optimally at 35-37°C, but also at 4°C
  • Associated with unwashed lettuce, deli meats, and vulnerable individuals (e.g., pregnant women)

Clostridium spp.

  • Gram-positive, straight or curved rods, forming chains
  • Spore former, motile with peritrichous flagella
  • Obligate anaerobes, saccharolytic, and proteolytic
  • Catalase-negative, with some species oxygen-tolerant or dying in oxygen

Test your knowledge of the characteristics and properties of Escherichia coli and Campylobacter bacteria. Learn about their gram stain results, motility, and other key features.

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