Bacterial Structure and Plasmids Quiz
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Questions and Answers

What is a key characteristic of transmissible plasmids?

  • They are transferred between cells by conjugation. (correct)
  • They contain only a single gene.
  • They are present in many copies per cell.
  • They are small and easily degraded.
  • What is the molecular weight range of non-transmissible plasmids?

  • 3–20 million (correct)
  • 10–30 million
  • 20–40 million
  • 40–100 million
  • What function is NOT associated with the genes carried by plasmids?

  • Resistance to heavy metals
  • Synthesis of sex pilus
  • DNA replication (correct)
  • Production of exotoxins
  • Which enzyme is involved in the resistance to heavy metals mediated by plasmids?

    <p>Reductase (B)</p> Signup and view all the answers

    What ability do transposons possess?

    <p>Movement within host cells (C), Coding for metabolic enzymes (D)</p> Signup and view all the answers

    Which structure is covered by a gelatinous layer in bacteria?

    <p>Capsule (B)</p> Signup and view all the answers

    Which among the following is a function of pili in bacteria?

    <p>Adherence to epithelial cells (D)</p> Signup and view all the answers

    How many copies of transmissible plasmids are usually present per cell?

    <p>1–3 (A)</p> Signup and view all the answers

    What are the three basic shapes into which bacteria are classified?

    <p>Cocci, bacilli, and spirochetes (C)</p> Signup and view all the answers

    Which structure is common to all bacteria except Mycoplasma?

    <p>Cell wall (C)</p> Signup and view all the answers

    What provides structural support and maintains the characteristic shape of bacterial cells?

    <p>Peptidoglycan (D)</p> Signup and view all the answers

    What type of bacteria typically have a thick peptidoglycan layer in their cell wall?

    <p>Gram-positive bacteria (B)</p> Signup and view all the answers

    Which of the following structures is external to the bacterial cell wall?

    <p>Capsule (B)</p> Signup and view all the answers

    What type of bacteria is characterized by having a relaxed coil shape?

    <p>Spirochetes (C)</p> Signup and view all the answers

    What is the primary function of peptidoglycan in bacteria?

    <p>Structural support (B)</p> Signup and view all the answers

    Which of the following does NOT describe features of cocci bacteria?

    <p>Variable in shape like pleomorphic bacteria (D)</p> Signup and view all the answers

    What is the primary role of mesosomes during cell division?

    <p>Serve as the origin of the transverse septum (B)</p> Signup and view all the answers

    Which of the following correctly describes the structure of bacterial ribosomes?

    <p>70S with 50S and 30S subunits (A)</p> Signup and view all the answers

    What is the key difference between bacterial DNA and eukaryotic DNA?

    <p>Bacterial DNA is circular. (C)</p> Signup and view all the answers

    What characterizes the nucleoid region of prokaryotic cells?

    <p>It contains DNA without histones. (D)</p> Signup and view all the answers

    What role do plasmids play in bacteria?

    <p>They replicate independently of the bacterial chromosome. (D)</p> Signup and view all the answers

    Which statement accurately describes the cytoplasm of prokaryotic cells?

    <p>It has two distinct areas including the nucleoid. (D)</p> Signup and view all the answers

    What is a notable characteristic of bacterial granules?

    <p>They serve as storage areas for nutrients. (A)</p> Signup and view all the answers

    How do antibiotics selectively inhibit bacterial protein synthesis?

    <p>They target the smaller size and composition differences of bacterial ribosomes. (A)</p> Signup and view all the answers

    What is the main function of penicillin binding proteins (PBP)?

    <p>To facilitate the binding of transpeptidases during peptidoglycan synthesis (D)</p> Signup and view all the answers

    What effect does penicillin have on the transpeptidase enzyme?

    <p>It irreversibly binds to its active site, inhibiting function (C)</p> Signup and view all the answers

    Which of the following statements is true regarding teichoic acids?

    <p>They can induce septic shock similar to endotoxins (C)</p> Signup and view all the answers

    Which component of the gram-negative cell wall is responsible for its endotoxin properties?

    <p>Lipopolysaccharide (LPS) (C)</p> Signup and view all the answers

    What is the role of lipopolysaccharides in gram-negative bacteria?

    <p>They trigger inflammatory responses leading to fever and shock (A)</p> Signup and view all the answers

    What distinguishes exotoxins from endotoxins in bacteria?

    <p>Exotoxins are usually proteins, whereas endotoxins are lipopolysaccharides (B)</p> Signup and view all the answers

    What is the primary composition of teichoic acids?

    <p>Polymers of glycerol phosphate or ribitol phosphate (D)</p> Signup and view all the answers

    How do penicillin and other beta-lactam antibiotics affect gram-positive bacteria?

    <p>They prevent transpeptidase from binding to D-alanine (C)</p> Signup and view all the answers

    What is the main role of pili in bacteria?

    <p>They mediate the attachment of bacteria to specific receptors on human cells. (A)</p> Signup and view all the answers

    Which of the following bacteria is known to form pili and has nonpathogenic mutants?

    <p>Neisseria gonorrhoeae (A)</p> Signup and view all the answers

    What is the primary function of the glycocalyx in bacteria?

    <p>To enable bacteria to adhere firmly to surfaces. (C)</p> Signup and view all the answers

    Which type of bacterial structure is specifically formed in response to adverse conditions?

    <p>Endospores (B)</p> Signup and view all the answers

    From which genera do medically important gram-positive rods that form spores originate?

    <p>Bacillus and Clostridium (D)</p> Signup and view all the answers

    What does the endospore primarily contain?

    <p>Bacterial DNA, peptidoglycan, and a thick coat (A)</p> Signup and view all the answers

    Which bacteria are primarily responsible for dental plaque formation?

    <p>Streptococcus mutans (B)</p> Signup and view all the answers

    What is unique about the sex pilus?

    <p>It forms the attachment during bacterial conjugation. (B)</p> Signup and view all the answers

    What is the primary composition of the capsule in most bacteria?

    <p>Polysaccharide (A)</p> Signup and view all the answers

    How many different serologic types of Streptococcus pneumoniae are recognized?

    <p>84 (A)</p> Signup and view all the answers

    What effect does the capsule have on phagocyte activity?

    <p>Limits phagocyte engulfment (D)</p> Signup and view all the answers

    What is the purpose of the Quellung reaction?

    <p>To identify bacterial capsules (A)</p> Signup and view all the answers

    What type of structures are flagella classified as?

    <p>Long, whiplike appendages (D)</p> Signup and view all the answers

    Which type of bacteria is more likely to have flagella?

    <p>Rods (C)</p> Signup and view all the answers

    What term describes the movement of bacteria towards nutrients using flagella?

    <p>Chemotaxis (A)</p> Signup and view all the answers

    In what way can flagella contribute to pathogenicity for certain bacteria?

    <p>By propelling bacteria into host tissues (B)</p> Signup and view all the answers

    Flashcards

    Cell Wall

    The outermost layer of bacterial cells that is composed of peptidoglycan and provides structural support, maintaining the characteristic shape of the cell.

    Peptidoglycan

    A unique molecule found only in bacterial cell walls, composed of glycan chains linked by peptide bonds, providing rigidity and shape maintenance.

    Gram Staining

    Gram-positive bacteria have a thick layer of peptidoglycan, making them stain purple in Gram staining. Gram-negative bacteria have a thin layer of peptidoglycan and an outer membrane, making them stain pink.

    Outer Membrane

    The outermost layer of Gram-negative bacteria, composed of phospholipids and lipopolysaccharide, providing an additional barrier and protection.

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    Cell Membrane

    The innermost layer of the bacterial cell that encloses the cytoplasm. It is involved in various cellular processes, including energy production and transport.

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    Cocci

    Round-shaped bacteria.

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    Bacilli

    Rod-shaped bacteria.

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    Spirochetes

    Spiral-shaped bacteria.

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    Transmissible Plasmids

    Large plasmids that contain genes for transferring themselves between bacteria. They are responsible for the synthesis of the sex pilus and enzymes required for transfer.

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    Non-Transmissible Plasmids

    Small plasmids that lack the genes for transfer, meaning they cannot move between cells. They are often present in multiple copies within a single bacterial cell.

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    Antibiotic resistance genes on plasmids

    Specific genes carried by plasmids that provide immunity to specific antibiotics. These genes encode for enzymes that neutralize or inactivate the antibiotic.

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    Heavy Metal Resistance Genes on plasmids

    Genes found on plasmids that confer resistance to heavy metals like mercury and silver. Usually encode for enzymes that remove or reduce the heavy metals inside the bacteria.

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    UV Light Resistance Genes on plasmids

    Genes carried by plasmids that allow the bacteria to survive exposure to ultraviolet (UV) light. These genes produce DNA repair enzymes.

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    Pili (Fimbriae) Genes on plasmids

    Genes found on plasmids that code for pili (fimbriae), hair-like structures that extend outward from the bacterial cell and allow them to attach to cell surfaces. These pili play a crucial role in bacterial infections.

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    Exotoxin Genes on plasmids

    Genes carried by plasmids that produce exotoxins, including enterotoxins which cause intestinal discomfort and illness.

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    Transposons

    Pieces of DNA that can move freely within and between bacterial genomes, plasmids, and bacteriophages. These 'jumping genes' can carry genes for antibiotic resistance, toxins, and metabolic enzymes.

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    Transpeptidases

    Enzymes that catalyze the formation of peptide cross-links in peptidoglycan, essential for bacterial cell wall synthesis.

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    Endopeptidases

    A class of enzymes that break down peptide bonds within a protein chain.

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    Penicillin-Binding Proteins (PBPs)

    Penicillin-binding proteins (PBPs) are essential bacterial enzymes involved in peptidoglycan synthesis. They are the primary targets for beta-lactam antibiotics like penicillin.

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    Mechanism of Action of Beta-lactam Antibiotics

    Beta-lactam antibiotics like penicillin bind to and inhibit the active site of transpeptidases, blocking the formation of peptide cross-links in peptidoglycan. This disrupts bacterial cell wall synthesis, leading to cell death.

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    Teichoic Acids

    Teichoic acids are polymers found in the cell walls of gram-positive bacteria, contributing to their structural integrity and mediating attachment to host cells. They can also trigger immune responses.

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    Lipopolysaccharide (LPS)

    Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. It acts as an endotoxin, triggering immune responses and contributing to disease pathogenesis.

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    Endotoxins

    Endotoxins are toxins that are an integral part of the bacterial cell wall, unlike exotoxins, which are secreted.

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    Outer Membrane of Gram-Negative Bacteria

    The outer membrane of gram-negative bacteria is composed of a phospholipid bilayer containing lipopolysaccharide (LPS), which contributes to its structural integrity and plays a crucial role in immune responses.

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    Capsule

    The outermost layer of a bacterial cell, composed of polysaccharides, that protects the cell from its environment.

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    Quellung reaction

    A reaction that occurs when a bacterium's capsule swells in the presence of specific antibodies. This reaction is used for identifying bacteria.

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    Flagella

    Long, whip-like structures that allow bacteria to move towards nutrients and other attractive substances.

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    Chemotaxis

    The process by which bacteria move in response to chemical signals, such as nutrients or toxins.

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    Spirochete movement

    The movement of bacteria using a flagellum-like structure called the axial filament, which wraps around the cell causing a wave-like motion.

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    Flagellar (H antigen)

    A type of antigen found on some bacteria that is used to identify them.

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    Virulence

    The ability of a bacterium to cause disease, which is often influenced by the presence of a capsule that helps it evade the immune system.

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    Capsular polysaccharide vaccines

    Capsular polysaccharides can be used to make vaccines against certain bacterial diseases.

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    What is a mesosome?

    An invagination of the cytoplasmic membrane that plays a role in cell division by forming the transverse septum and serving as a binding site for DNA.

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    What is the cytoplasm of a bacterial cell?

    The gel-like substance within the cell, containing ribosomes, nutrients, metabolites, and plasmids. It also houses the nucleoid region, the DNA-containing area.

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    What are ribosomes and how do they differ in bacteria and eukaryotes?

    Small structures responsible for protein synthesis. Bacterial ribosomes are 70S, with 50S and 30S subunits, compared to eukaryotic ribosomes which are 80S.

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    Explain the selective action of antibiotics on bacterial ribosomes.

    The differences in ribosomal RNAs and proteins allow certain antibiotics to specifically inhibit bacterial protein synthesis, leaving human cells unaffected.

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    What are granules in the cytoplasm?

    Storage areas for nutrients within the cytoplasm, often staining differently with specific dyes.

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    What is the nucleoid of a bacterium?

    The region in the cytoplasm where DNA is located. It lacks a nuclear membrane, a nucleolus, and histones, making it different from a eukaryotic nucleus.

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    What are plasmids and where are they found?

    Extrachromosomal, circular DNA molecules that replicate independently of the bacterial chromosome. They can integrate into the chromosome and exist in both Gram-positive and Gram-negative bacteria.

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    Compare the bacterial DNA to human DNA.

    Bacteria have a single, circular DNA molecule with approximately 2000 genes, whereas human DNA has around 100,000 genes.

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    Pili (Fimbriae)

    Hair-like filaments extending from the bacterial cell surface, shorter and straighter than flagella, mainly found in gram-negative bacteria. Key roles include mediating bacterial attachment to specific receptors on human cells and forming the connection between donor and recipient bacteria during conjugation.

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    Glycocalyx (Slime Layer)

    A polysaccharide coating secreted by many bacteria. It acts like a film, helping bacteria adhere to various surfaces like skin, heart valves, and catheters. It's crucial for some bacteria, like Streptococcus mutans, to stick to teeth, contributing to plaque formation.

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    Spores

    Highly resistant structures formed inside certain bacteria (Bacillus and Clostridium) in response to harsh conditions. They contain bacterial DNA, a small amount of cytoplasm, and a thick, keratin-like coat, allowing survival in extreme environments.

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    Sex Pilus

    A specialized pilus involved in bacterial conjugation, forming the connection between the donor and recipient bacteria during the transfer of genetic material.

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    Flagellar Motility

    A type of bacterial movement that involves the rotation of flagella, allowing the bacteria to move towards or away from stimuli like food or toxins.

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    Adhesive Pili

    A specialized pilus that allows bacteria to adhere to surfaces, crucial for colonization and infection. It's a major factor in bacterial pathogenicity.

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    Sporulation

    The process of spore formation in bacteria, triggered by adverse conditions like nutrient depletion. It involves the encapsulation of genetic material and essential components within a resistant spore.

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    Twitching Motility

    A type of bacterial motility where bacteria move across surfaces by extending and retracting pili. It's different from flagellar motility and often involved in surface colonization and biofilm formation.

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    Study Notes

    Bacterial Structure

    • Bacteria are classified by shape into three basic groups: cocci, bacilli, and spirochetes.
    • Cocci are round, bacilli are rod-shaped, and spirochetes are spiral-shaped.
    • Some bacteria are variable in shape and are said to be pleomorphic.

    Learning Objectives

    • Students should be able to define typical bacteria.
    • Bacterial morphology (appearance)
    • Cell wall structure, peptidoglycans.
    • Cell wall in gram-positive and gram-negative bacteria.
    • Cell membrane, mesosome, ribosome, genetic material.
    • Specialized structures outside the cell wall: capsule, pili, flagella, glycocalyx

    Structure of Bacterial Cells

    • Bacteria are classified by three basic shapes: cocci, bacilli, and spirochetes.
    • Cocci are round; bacilli are rod-shaped; spirochetes are spiral-shaped.
    • Some bacteria are variable in shape and are said to be pleomorphic (many-shaped).

    Bacterial Morphology

    • Cocci: grouped in clusters (Staphylococcus), chains (Streptococcus), pairs (Streptococcus pneumoniae), pairs with pointed ends (Neisseria - kidney bean shape).
    • Rods (bacilli): square ends (Bacillus), rounded ends (Salmonella), club-shaped (Corynebacterium), fusiform (Fusobacterium), comma-shaped (Vibrio).
    • Spirochetes: relaxed coil Borrelia; tightly coiled Treponema.

    Structure

    • Cytoplasm
    • Ribosomes
    • Cell membrane
    • Cell wall
    • Capsule
    • Flagella
    • Attachment pili
    • Plasmid
    • Sex pilus

    Cell Wall

    • The cell wall is the outermost component of bacteria (except Mycoplasma).
    • Some bacteria have surface features (capsule, flagella, pili) external to the cell wall.
    • The cell wall is composed of an inner layer of peptidoglycan and an outer membrane (whose thickness and composition vary between bacteria types).
    • Peptidoglycan provides structural support and maintains the shape of the cell.
    • The cell wall protects from osmotic pressure.

    Peptidoglycan

    • Also called murein or mucopeptide.
    • Found only in bacterial walls.
    • Provides rigid support to the cell.
    • Important in maintaining the characteristic shape of the cell.
    • Composed of glycan chains connected by peptide bonds.
    • Repeating sugars: N-acetyl-muramic acid (NAM) and N-acetyl-glucosamine (NAG) connected by beta 1,4-glycoside bonds.
    • Target region of lysozyme.

    Gram-positive Cell Wall

    • Thick peptidoglycan layer.
    • Glycan strings are tightened with teichoic acid strings.

    Gram-negative Cell Wall

    • Thin peptidoglycan layer.
    • Outer membrane (with lipopolysaccharide (LPS)).
    • Cytoplasmic membrane.
    • Periplasmic space.

    Teichoic Acid

    • Present only in gram-positive bacteria.
    • Activates the same pathways as lipopolysaccharide (LPS) in gram-negative bacteria.
    • Phage receptors in gram-negative bacteria.
    • Composed of polymers of glycerol phosphate or ribitol phosphate.

    Medical Importance of Teichoic Acid

    • Ability to induce septic shock.
    • Gram-positive bacteria activate the same pathways as does endotoxin (LPS) in gram-negative bacteria.
    • Mediates attachment of staphylococci to mucosal cells.

    Gram-negative Cell Wall

    • Composed of an outer membrane.
    • Thin peptidoglycan layer is between the cytoplasmic membrane and outer membrane.
    • Contains lipopolysaccharide (LPS).
    • Contains porin proteins (OMPs).

    Outer Membrane

    • Inner and outer layers form a bilayered membrane that are different from each other.
    • Outermost phospholipid layer contains a lot of lipopolysaccharide molecules.

    Exotoxin and Endotoxin

    • Lipopolysaccharide (LPS) of gram-negative bacteria is endotoxin.
    • Responsible for disease features like fever and shock.
    • Endotoxin is part of the cell wall.
    • Exotoxin is released from bacteria.
    • Pathologic effects of endotoxin are similar regardless of the organism.

    Composition of LPS

    • Composed of three molecules: lipid A (toxin), core polysaccharide, an outer polysaccharide layer.
    • Core polysaccharide is 5 sugars linked to lipid A.
    • Outer polysaccharide is 25 repeating units of 3-5 sugars; this outer polymer is the somatic (O antigen).

    Porin Proteins

    • Outer membrane contains protein structures (porins).
    • Porins are channels for substances.
    • OMPs (outer membrane porins).
    • Phage receptors in gram-negative bacteria.

    Acid-Fast and Associated Cell Wall

    • Cell walls of mycobacteria and Nocardia are thick and rich in lipids (mycolic acid).
    • These bacteria resist decolorization with acid-alcohol after being stained with carbolfuchsin.
    • Resistance is due to high concentration of mycolic acids in the cell wall.

    L-form Bacteria

    • Cell wall-deficient (CWD) bacteria.
    • Strains of bacteria lack cell walls.
    • Bacteria may survive if cell wall structures are lost when exposed to lysozyme or antibiotics.

    Transpeptidation

    • Transpeptidase, endopeptidase, and carboxypeptidase create cell walls.
    • Penicillin binding proteins (PBPs) are targets for beta-lactam antibiotics.

    Effect on Cell Wall

    • Beta-lactam antibiotics (penicillin) show molecular similarity and bind with PBP.
    • Irreversibly binds to transpeptidase enzyme active site.
    • Penicillin prevents enzyme from binding with D-ala.
    • Peptidoglycan synthesis is inhibited.

    Cytoplasmic Membrane

    • Just inside the peptidoglycan layer.
    • Phospholipid bilayer.
    • Contains sterols in eukaryotic cells, but prokaryotes generally do not (except for Mycoplasma).
    • Active transport of molecules.
    • Energy generation by oxidative phosphorylation.
    • Synthesis of precursors of the cell wall.
    • Secretion of enzymes and toxins.

    Bacterial Cytoplasmic Membrane

    • Glycolipid and hopanoid chains.
    • Integral and peripheral proteins.
    • Only on one side.

    Mesosome

    • Invagination of the cytoplasmic membrane.
    • Important during cell division.
    • Origin of transverse septum that divides the cell in half.
    • Binding site for DNA, forms genetic material for daughter cells.

    Cytoplasm

    • Two areas when viewed using electron microscope:
    • Amorphous matrix containing ribosomes, nutrient granules, metabolites, and plasmids.
    • Inner, nucleoid region composed of DNA.

    Ribosomes

    • Site of protein synthesis.
    • Differ from eukaryotic ribosomes in size and composition.
    • Bacterial ribosomes are 70S (50S + 30S subunits).
    • Eukaryotic ribosomes are 80S (60S + 40S subunits).

    Important Note

    • Differences in ribosomal RNAs and proteins are the basis of selective antibiotic action (in bacteria, not humans).

    Granules

    • Cytoplasm contains various granules for nutrient storage.
    • Granules stain characteristically with certain dyes.

    Nucleoid

    • Area of cytoplasm containing DNA.
    • DNA of prokaryotes is single, circular molecule.
    • Its molecular weight is approximately 2 x 10⁹ and contains approximately 2000 genes.
    • No nuclear membrane, nucleolus, mitotic spindle, or histones.
    • No resemblance to eukaryotic nucleus.
    • Bacterial (prokaryotic) DNA has no introns.

    Plasmids

    • Extrachromosomal, double-stranded, circular DNA molecules.
    • Replicate independently from the bacterial chromosome.
    • Can be integrated into the bacterial chromosome.
    • Occur in both gram-positive and gram-negative bacteria.
    • Several types can exist in one cell.

    Transmissible Plasmids

    • Can be transferred from one cell to another via conjugation.
    • Large (MW 40-100 million), containing many genes for sex pilus and transfer enzymes.
    • Typically present in one to three copies per cell.

    Non-transmissible Plasmids

    • Small (MW 3–20 million).
    • Do not contain transfer genes.
    • Present in many copies (10–60) per cell.

    Important Plasmid Genes

    • Carry genes for antibiotic resistance (mediated by various enzymes).
    • Resistance to heavy metals (e.g., mercury).
    • Resistance to ultraviolet light (mediated by DNA repair enzymes).
    • Pilus formation (mediates adherence to epithelial cells).
    • Production of exotoxins (e.g., enterotoxins).

    Transposons

    • Pieces of DNA that move readily within or between bacterial DNA, plasmids, and bacteriophages.
    • May code for drug-resistance enzymes, toxins, or metabolic enzymes.
    • Not capable of independent replication

    Specialized Structures

    • Capsule
    • Flagella
    • Pili
    • Glycocalyx

    Capsule

    • Gelatinous layer surrounding entire bacteria, except anthrax bacillus, which has a polymerized D-glutamic acid capsule.
    • Sugar components vary between species and help determine the serologic type within a species.
    • Important determinant of virulence: limits phagocyte engulfment.
    • Used in microbial identification (e.g., quellung reaction).
    • Component of some vaccines (e.g., Streptococcus pneumoniae).
    • Aids in bacterial adherence to human tissues (initial step in infection).

    Flagella

    • Long, whip-like appendages.
    • Move bacteria toward nutrients and attractants (chemotaxis).
    • Varying numbers and locations (monotrichous, lophotrichous, amphitrichous, peritrichous).
    • Important in pathogenesis (e.g., urinary tract infections; propelling bacteria up the urethra into the bladder).
    • Identification of bacteria in laboratory using H antigens.

    Pili (Fimbriae)

    • Hair-like filaments extending from the cell surface.
    • Shorter and straighter than flagella.
    • Commonly found on gram-negative bacteria.
    • Mediate attachment of bacteria to human cell surfaces (e.g., in Neisseria gonorrhoeae).
    • Pilus formation important for bacterial pathogenicity.

    Glycocalyx (Slime Layer)

    • Polysaccharide coating secreted by bacteria.
    • Allows bacteria to adhere to surfaces (e.g., skin, heart valves, catheters).
    • Plays a role in formation of plaque (precursor to dental caries), especially in Streptococcus mutans.

    Spores

    • Highly resistant structures formed in response to adverse conditions (nutrient depletion and chemicals).
    • Present in two medically important genera of gram-positive rods: Bacillus and Clostridium.
    • Contain bacterial DNA, cytoplasm, cell membrane, and peptidoglycan.
    • Contain very little water and a thick, keratin-like layer.

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