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Questions and Answers
Bacterial ______ is necessary to make them readily visible for observation of intracellular structures
staining
Negative staining is good for viewing bacterial ______
capsules
Negative, indirect, or background staining is achieved by mixing bacteria with an acidic ______
stain
Stains like nigrosin, India ink, or eosin produce a deposit around the bacteria or a dark ______
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Negative staining makes bacteria appear as unstained cells with a clear area around them due to repulsion between the negative charge of the stains and the negatively charged bacterial ______
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Negative staining allows determination of overall bacterial morphology without the use of harsh staining or heat-fixing techniques that change the shape of ______
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Study Notes
Bacterial Staining
- Bacterial staining is necessary to make bacteria visible for observation of intracellular structures and overall morphology because living bacteria are generally colorless and invisible.
Types of Bacterial Staining
- Negative stain: used to determine overall bacterial morphology without using harsh staining or heat-fixing techniques.
- Simple stain: no specific information provided.
- Differential stain: no specific information provided.
- Gram stain: no specific information provided.
- Capsule stain: no specific information provided.
- Acid-fast stain: no specific information provided.
- Endospore stain: no specific information provided.
- Flagella stain: no specific information provided.
Negative Staining
- Principles:
- Achieved by mixing bacteria with an acidic stain (e.g., nigrosin, India ink, or eosin).
- The acidic stain does not penetrate and stain the bacterial cells due to repulsion between the negative charge of the stains and the negatively charged bacterial wall.
- Instead, the stain produces a deposit around the bacteria or a dark background, making the bacteria appear as unstained cells with a clear area around them.
- Procedure: as shown in Figure 1.
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Description
Test your knowledge of bacterial staining techniques including negative stain, simple stain, differential stain, Gram stain, capsule stain, acid-fast stain, endospore stain, and flagella stain. Understand the importance of staining in making bacterial structures visible for observation.
