Podcast
Questions and Answers
What is the first step in preparing a smear for staining?
What is the first step in preparing a smear for staining?
- Adding a loopful of water to the slide
- Heat-fixing the slide (correct)
- Adding the inoculum to the drop of water
- Applying the primary stain
What is the purpose of adding water to the slide when preparing a smear?
What is the purpose of adding water to the slide when preparing a smear?
- To create a suspension and spread the bacteria
- To fix the bacteria to the slide
- To kill the bacteria (correct)
- To dilute the stain
After air drying a smear, what is the next step?
After air drying a smear, what is the next step?
- Rinsing with water
- Adding the stain
- Cover slipping (correct)
- Heat fixing
How many times should you pass the slide through the flame during heat fixing?
How many times should you pass the slide through the flame during heat fixing?
What is the primary purpose of heat fixing a smear?
What is the primary purpose of heat fixing a smear?
In Gram staining, what color will Gram-positive cells appear?
In Gram staining, what color will Gram-positive cells appear?
Which step in Gram staining involves using alcohol?
Which step in Gram staining involves using alcohol?
What is the function of safranin in Gram staining?
What is the function of safranin in Gram staining?
What is the purpose of the mordant in Gram staining?
What is the purpose of the mordant in Gram staining?
What color do Gram-negative cells appear after Gram staining?
What color do Gram-negative cells appear after Gram staining?
What is the primary stain used in Gram staining?
What is the primary stain used in Gram staining?
Which of the following is a differential stain?
Which of the following is a differential stain?
If a cell retains the primary stain after being rinsed with acid-alcohol, it is called:
If a cell retains the primary stain after being rinsed with acid-alcohol, it is called:
What happens to non-acid-fast cells when rinsed with acid-alcohol?
What happens to non-acid-fast cells when rinsed with acid-alcohol?
Why is mycolic acid significant in acid-fast bacteria?
Why is mycolic acid significant in acid-fast bacteria?
The Ziehl-Neelsen method uses which of the following?
The Ziehl-Neelsen method uses which of the following?
What is the counterstain used in the Ziehl-Neelsen acid-fast staining method?
What is the counterstain used in the Ziehl-Neelsen acid-fast staining method?
In the Kinyoun method, what is the primary stain?
In the Kinyoun method, what is the primary stain?
What reagent is used to decolorize in both the Ziehl-Neelsen and Kinyoun methods?
What reagent is used to decolorize in both the Ziehl-Neelsen and Kinyoun methods?
Which of the following is a key difference between the Ziehl-Neelsen and Kinyoun methods?
Which of the following is a key difference between the Ziehl-Neelsen and Kinyoun methods?
What color are acid-fast cells after staining with the Ziehl-Neelsen method?
What color are acid-fast cells after staining with the Ziehl-Neelsen method?
What is the counterstain used in the Kinyoun method?
What is the counterstain used in the Kinyoun method?
What color do acid-fast cells appear after staining with the Kinyoun method?
What color do acid-fast cells appear after staining with the Kinyoun method?
What is a key characteristic of acid-fast positive bacteria's cell walls?
What is a key characteristic of acid-fast positive bacteria's cell walls?
Which of the following is an example of an acid-fast positive bacteria?
Which of the following is an example of an acid-fast positive bacteria?
Mycobacterium tuberculosis causes which disease?
Mycobacterium tuberculosis causes which disease?
Which component of Mycobacterium is a significant determinant of virulence?
Which component of Mycobacterium is a significant determinant of virulence?
Mycobacterium leprae is the causative agent of which disease?
Mycobacterium leprae is the causative agent of which disease?
Which of the following is true regarding acid-fast staining?
Which of the following is true regarding acid-fast staining?
Which of the following is a non-pathogenic Mycobacterium species?
Which of the following is a non-pathogenic Mycobacterium species?
What is the first step in the Ziehl-Neelsen staining procedure?
What is the first step in the Ziehl-Neelsen staining procedure?
During Ziehl-Neelsen staining, how long should you maintain steaming after adding carbol fuchsin?
During Ziehl-Neelsen staining, how long should you maintain steaming after adding carbol fuchsin?
What is the function of acid-alcohol in acid-fast staining?
What is the function of acid-alcohol in acid-fast staining?
Which of the following best describes acid-fast bacteria?
Which of the following best describes acid-fast bacteria?
Which stain is used to identify bacteria with waxy cell walls?
Which stain is used to identify bacteria with waxy cell walls?
What is added to the slide to create a suspension?
What is added to the slide to create a suspension?
Flashcards
Smear Preparation
Smear Preparation
A technique used to create a suspension to spread bacteria on a slide for staining and observation.
Water's role in Smear Prep
Water's role in Smear Prep
Adding a small amount of water to the slide to create a medium for the bacteria to spread.
Inoculum
Inoculum
The material containing microorganisms that is added to the water on the slide.
Heat Fixation
Heat Fixation
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Differential Stain
Differential Stain
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Acid-Fast Stain
Acid-Fast Stain
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Mycolic Acid
Mycolic Acid
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Carbol Fuchsin
Carbol Fuchsin
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Ziehl-Neelsen Method
Ziehl-Neelsen Method
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Methylene Blue
Methylene Blue
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Kinyoun Method
Kinyoun Method
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Acid-Fast Positive Result
Acid-Fast Positive Result
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M. tuberculosis
M. tuberculosis
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M. leprae
M. leprae
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Mycolic Acids
Mycolic Acids
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Gram Staining
Gram Staining
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Study Notes
- To prepare a bacterial smear, a loopful of water added to a slide creates a suspension that helps spread bacteria.
- Inoculum is added to the water on the slide to form a suspension.
- The slide is then allowed to air dry.
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Description
Creating a bacterial smear involves suspending bacteria in water on a slide. This suspension aids in evenly spreading the inoculum. The prepared slide is then allowed to air dry completely before further processing.
