Bacterial Plasmids Theory
12 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary function of recombinant plasmids in genetic modification?

To synthesise specific proteins in bacteria.

What is the process by which bacteria take up recombinant plasmids from the environment?

Bacterial transformation.

What is the significance of plasmid DNA in bacteria?

It replicates independently from the circular chromosome, allowing genetic modification.

What is the purpose of editing a plasmid to incorporate a target gene of interest?

<p>To create a recombinant plasmid that synthesises specific proteins.</p> Signup and view all the answers

What is the result of bacterial transformation in the medical and food industries?

<p>Cheaper and more efficient methods of production.</p> Signup and view all the answers

What is the variable characteristic of plasmids in bacteria?

<p>Length, which can be up to 200 kbp.</p> Signup and view all the answers

What is the purpose of introducing a gene of interest into a plasmid vector, and what happens to the bacteria that take up the recombinant plasmid?

<p>The purpose is to produce a protein encoded by the foreign DNA. The bacteria will express the protein encoded by that foreign DNA.</p> Signup and view all the answers

What is the significance of the genetic code being universal in recombinant DNA technology?

<p>It allows bacteria to use the DNA to synthesise an identical protein, despite the gene of interest coming from another organism.</p> Signup and view all the answers

Why is it important to ensure the gene of interest does not have introns prior to insertion into a plasmid vector?

<p>Because bacteria would not know what to do with intron segments, as prokaryotic gene expression does not involve RNA processing like in eukaryotes.</p> Signup and view all the answers

What is bacterial transformation, and how is it used in recombinant DNA technology?

<p>Bacterial transformation is the process by which bacteria take up foreign DNA from their environment. Scientists use this process to introduce recombinant plasmids into bacteria.</p> Signup and view all the answers

What is the purpose of using antibiotic resistance genes in plasmid vectors?

<p>To make it easy to select and identify bacteria that have taken up the recombinant plasmid.</p> Signup and view all the answers

What is an example of a protein that can be produced using recombinant DNA technology, and what is its use?

<p>Insulin, which is used to manage diabetes.</p> Signup and view all the answers

More Like This

Bacterial Nucleoid and Plasmids
30 questions
Bacterial Genetics and DNA Replication
18 questions
Use Quizgecko on...
Browser
Browser