12 Questions
What is the primary function of recombinant plasmids in genetic modification?
To synthesise specific proteins in bacteria.
What is the process by which bacteria take up recombinant plasmids from the environment?
Bacterial transformation.
What is the significance of plasmid DNA in bacteria?
It replicates independently from the circular chromosome, allowing genetic modification.
What is the purpose of editing a plasmid to incorporate a target gene of interest?
To create a recombinant plasmid that synthesises specific proteins.
What is the result of bacterial transformation in the medical and food industries?
Cheaper and more efficient methods of production.
What is the variable characteristic of plasmids in bacteria?
Length, which can be up to 200 kbp.
What is the purpose of introducing a gene of interest into a plasmid vector, and what happens to the bacteria that take up the recombinant plasmid?
The purpose is to produce a protein encoded by the foreign DNA. The bacteria will express the protein encoded by that foreign DNA.
What is the significance of the genetic code being universal in recombinant DNA technology?
It allows bacteria to use the DNA to synthesise an identical protein, despite the gene of interest coming from another organism.
Why is it important to ensure the gene of interest does not have introns prior to insertion into a plasmid vector?
Because bacteria would not know what to do with intron segments, as prokaryotic gene expression does not involve RNA processing like in eukaryotes.
What is bacterial transformation, and how is it used in recombinant DNA technology?
Bacterial transformation is the process by which bacteria take up foreign DNA from their environment. Scientists use this process to introduce recombinant plasmids into bacteria.
What is the purpose of using antibiotic resistance genes in plasmid vectors?
To make it easy to select and identify bacteria that have taken up the recombinant plasmid.
What is an example of a protein that can be produced using recombinant DNA technology, and what is its use?
Insulin, which is used to manage diabetes.
This quiz covers the details about bacterial plasmids, including their replication process, variations in number and length, and characteristics. It also includes information about plasmid DNA being separate from the chromosome and its typical presence in bacteria.
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