Podcast
Questions and Answers
What is the main impact of antimicrobial resistance (AMR) on healthcare globally?
What is the main impact of antimicrobial resistance (AMR) on healthcare globally?
AMR negatively impacts healthcare globally due to its deleterious effects on combating infections, necessitating alternate treatments and impacting cost and access to care.
What is the role of Clinical Microbiology Laboratories (CMLs) in combating AMR?
What is the role of Clinical Microbiology Laboratories (CMLs) in combating AMR?
CMLs play a crucial role in combating AMR by providing accurate identification of pathogens and their susceptibility to various antimicrobial drugs, facilitating effective treatment and informed decisions for public health interventions.
What is Proficiency Testing (PT) and why is it important in CMLs?
What is Proficiency Testing (PT) and why is it important in CMLs?
PT is a valuable and important activity for CMLs to engage in. It involves testing a laboratory’s performance in identifying and testing organisms and their susceptibility to drugs, ensuring reliability, accuracy and proficiency in their services.
In the context of CMLs, the PT program in Lebanon’s Ministry of Public Health (MOPH) was made mandatory for all CM services in 2015.
In the context of CMLs, the PT program in Lebanon’s Ministry of Public Health (MOPH) was made mandatory for all CM services in 2015.
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What is the EUCAST? What is its role in antimicrobial susceptibility testing?
What is the EUCAST? What is its role in antimicrobial susceptibility testing?
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What is the importance of the Standard Operating Procedures (SOP) provided during the PT program?
What is the importance of the Standard Operating Procedures (SOP) provided during the PT program?
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In the PT program, how were the bacteria distributed to each laboratory for identification and AMST?
In the PT program, how were the bacteria distributed to each laboratory for identification and AMST?
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Which methods were employed to perform antimicrobial susceptibility testing (AMST) on the distributed isolates?
Which methods were employed to perform antimicrobial susceptibility testing (AMST) on the distributed isolates?
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In the given text, what were the scoring criteria used to evaluate laboratories in terms of their performance in bacterial identification?
In the given text, what were the scoring criteria used to evaluate laboratories in terms of their performance in bacterial identification?
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In the given text, how were the scores for antimicrobial susceptibility testing (AMST) calculated?
In the given text, how were the scores for antimicrobial susceptibility testing (AMST) calculated?
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What were the overall scoring criteria used to evaluate individual laboratories in the PT program?
What were the overall scoring criteria used to evaluate individual laboratories in the PT program?
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What was the overall conclusion drawn from the PT activity in Lebanon regarding the performance of the CMLs?
What was the overall conclusion drawn from the PT activity in Lebanon regarding the performance of the CMLs?
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What were some of the positive aspects observed in the Lebanese PT activity?
What were some of the positive aspects observed in the Lebanese PT activity?
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List some of the concerns raised by labs regarding their ability to participate in the proficiency testing program?
List some of the concerns raised by labs regarding their ability to participate in the proficiency testing program?
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What were the recommendations provided by the authors to address the challenges faced by CMLs in Lebanon and improve their PT participation?
What were the recommendations provided by the authors to address the challenges faced by CMLs in Lebanon and improve their PT participation?
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Why is providing accurate and validated laboratory results essential for effective patient care and public health?
Why is providing accurate and validated laboratory results essential for effective patient care and public health?
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The PT activity in Lebanon revealed a consistent and strong performance among all participating laboratories in bacterial identification, antimicrobial susceptibility testing, and overall scores.
The PT activity in Lebanon revealed a consistent and strong performance among all participating laboratories in bacterial identification, antimicrobial susceptibility testing, and overall scores.
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Study Notes
Nationwide Proficiency Assessment of Bacterial Identification and Antimicrobial Susceptibility Testing
- A study assessed the proficiency of 110 Lebanese laboratories in bacterial identification and antimicrobial susceptibility testing (AMST).
- Four proficiency testing (PT) activities were conducted, involving 110 laboratories across Lebanon.
- 5 different bacterial species (S. aureus, Enterococcus faecalis, Listeria monocytogenes, Streptococcus agalactiae, and Corynebacterium amycolatum; Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi ESBL, Enterobacter aerogenes, and Stenotrophomonas maltophilia) were tested for identification and AMST.
- Significant variation in laboratory performance was observed across various bacterial species.
- The correct identification rate for S. aureus was 100%, while identification rates for other species varied from 7% to 97%.
- Correct AMST results demonstrated similar variation, ranging between 33% and 82%.
Methodology
- Pre-activity workshops were conducted to train laboratory staff in bacterial identification and AMST techniques.
- Standardized quality control procedures (CLSI guidelines) were followed.
- Materials like a summary brochure detailing standard operating procedures (SOPs) for the tests, were utilized.
- Bacterial isolates for testing were prepared.
- AMST was conducted using CLSI disk diffusion guidelines.
- Minimal inhibitory concentrations (MICs) for isolates were also determined using the Vitek 2 system.
Results
- Laboratories showed varying proficiency levels in bacterial identification and AMST.
- Overall performance scores were considerably low.
- A substantial number of laboratories were identified needing further training in bacterial identification and a greater emphasis on antibiotic susceptibility testing procedures.
- Repeat testing on a subset of laboratories indicated that in many cases, repeat results did not improve overall scores.
- The study highlighted the need for comprehensive professional development and continued monitoring of laboratory proficiency levels in Lebanon.
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Description
Test your knowledge on bacterial identification and antimicrobial susceptibility testing with this quiz focused on the proficiency assessment of Lebanese laboratories. Explore various bacterial species and their identification rates while understanding antimicrobial susceptibility. Challenge yourself with questions based on a nationwide study.