12 Questions
What is the primary reason bacterial cells do not increase in size indefinitely?
The cell wall becomes impaired when cells become too large
What is the primary mechanism by which bacterial populations increase in number?
Binary fission
Which of the following is a key requirement for successful binary fission in bacteria?
All of the above
What is the primary purpose of the standard plate count method in microbiology?
To determine the total number of viable bacteria in a sample
How does the spread plate method differ from the pour plate method in bacterial enumeration?
The spread plate method allows for better colony separation
Why is it important for bacterial cells to divide at the right time and place?
To remain viable and competitive
What is the purpose of performing serial dilutions?
To obtain a small number of colonies on each plate for accurate counting.
Which statement is true about the standard plate count method?
It only counts bacteria capable of growing on the specific culture medium and conditions used.
What assumption is made in the standard plate count method?
Each colony originates from a single bacterial cell.
What is the formula for calculating the dilution factor?
Dilution factor = Volume of sample / (Volume of sample + Volume of diluent)
What is the advantage of the pour plate method over the spread plate method?
It is faster and easier to perform.
How is the microbial population in the sample computed from the plate count?
Microbial population = Number of colonies / (Amount plated × Dilution factor)
Learn about how bacterial growth primarily occurs through cell division rather than an increase in cell size. Explore the limitations on cell size and the importance of cell division in maintaining a healthy bacterial population.
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