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12 Questions

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Bacterial Growth and Cell Division

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Questions and Answers

What is the primary reason bacterial cells do not increase in size indefinitely?

Bacterial cells rely on binary fission for propagation

Bacterial cells must divide at the right time and place to remain competitive

Bacterial cells have a limited capacity to support essential cellular components

The cell wall becomes impaired when cells become too large (correct)

What is the primary mechanism by which bacterial populations increase in number?

Binary fission (correct)

Mitosis and meiosis

Increase in cell size

Spectrophometry

Which of the following is a key requirement for successful binary fission in bacteria?

Giving each offspring a complete copy of its essential genetic material

Maintaining a stable cell size

Dividing at the right time and place

All of the above (correct)

What is the primary purpose of the standard plate count method in microbiology?

To determine the total number of viable bacteria in a sample Signup and view all the answers

How does the spread plate method differ from the pour plate method in bacterial enumeration?

The spread plate method allows for better colony separation Signup and view all the answers

Why is it important for bacterial cells to divide at the right time and place?

To remain viable and competitive Signup and view all the answers

What is the purpose of performing serial dilutions?

To obtain a small number of colonies on each plate for accurate counting. Signup and view all the answers

Which statement is true about the standard plate count method?

It only counts bacteria capable of growing on the specific culture medium and conditions used. Signup and view all the answers

What assumption is made in the standard plate count method?

Each colony originates from a single bacterial cell. Signup and view all the answers

What is the formula for calculating the dilution factor?

Dilution factor = Volume of sample / (Volume of sample + Volume of diluent) Signup and view all the answers

What is the advantage of the pour plate method over the spread plate method?

It is faster and easier to perform. Signup and view all the answers

How is the microbial population in the sample computed from the plate count?

Microbial population = Number of colonies / (Amount plated × Dilution factor) Signup and view all the answers