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Bacterial Genotypes and Gene Expression Quiz

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Bacterial Genotypes and Gene Expression Quiz

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Questions and Answers

What mutation removes both restriction and methylation from the host?

hsdSB(rB- mB-)

Which mutation specifically eliminates restriction at the sequence C mCGG?

mcrA183

What type of plasmid is the F plasmid in E.coli?

Episome

What is the size of the F (fertility) plasmid in E.coli approximately?

100 kb Signup and view all the answers

What is the function of the F plasmid in E.coli?

Codes a series of proteins for conjugative DNA transfer Signup and view all the answers

What is the purpose of placing the T7 gene1 under the control of the lac promotor in the prophage?

To control the expression of the T7 gene1 Signup and view all the answers

What role does the lac operon play in the SHuffle T7 Express strain?

Regulates protein expression Signup and view all the answers

Which strain is used for plasmid propagation with B/W selection and contains the following markers: hsdR514, supE44, supF58, lacY1, galK2, galT22, metB1, trp55?

DH5α Signup and view all the answers

What is the best solvent to use for quantitating nucleic acid by absorbance of light?

Water Signup and view all the answers

Why is the absorbance of light at 260nm used for quantitating nucleic acid?

It corresponds to absorbance by heterocyclic rings in DNA and RNA Signup and view all the answers

What is the main concern when quantitating nucleic acid at 280nm?

Interference from other compounds Signup and view all the answers

What does the genotype of a bacterial strain typically describe?

It tells you what the host can or cannot do. Signup and view all the answers

What is the significance of the notation 'galK2' in a bacterial genotype?

It indicates that the second mutation characterized in the galK gene, which is part of the gal operon involved in galactose usage. Signup and view all the answers

What is the purpose of quantitating DNA in molecular biology?

To determine the amount or concentration of DNA in a sample. Signup and view all the answers

What is the role of restriction enzymes in molecular biology?

They are used to cut DNA at specific sequences, facilitating DNA manipulation and analysis. Signup and view all the answers

How is the purity of DNA typically assessed?

Through the separation and visualization of DNA, often using techniques like agarose gel electrophoresis. Signup and view all the answers

What is the purpose of using bacterial strains like DH5α and SHuffle T7 Express in molecular biology?

They are used as hosts for protein expression and plasmid propagation. Signup and view all the answers