Podcast
Questions and Answers
What mutation removes both restriction and methylation from the host?
What mutation removes both restriction and methylation from the host?
hsdSB(rB- mB-)
Which mutation specifically eliminates restriction at the sequence C mCGG?
Which mutation specifically eliminates restriction at the sequence C mCGG?
mcrA183
What type of plasmid is the F plasmid in E.coli?
What type of plasmid is the F plasmid in E.coli?
Episome
What is the size of the F (fertility) plasmid in E.coli approximately?
What is the size of the F (fertility) plasmid in E.coli approximately?
What is the function of the F plasmid in E.coli?
What is the function of the F plasmid in E.coli?
What is the purpose of placing the T7 gene1 under the control of the lac promotor in the prophage?
What is the purpose of placing the T7 gene1 under the control of the lac promotor in the prophage?
What role does the lac operon play in the SHuffle T7 Express strain?
What role does the lac operon play in the SHuffle T7 Express strain?
Which strain is used for plasmid propagation with B/W selection and contains the following markers: hsdR514, supE44, supF58, lacY1, galK2, galT22, metB1, trp55?
Which strain is used for plasmid propagation with B/W selection and contains the following markers: hsdR514, supE44, supF58, lacY1, galK2, galT22, metB1, trp55?
What is the best solvent to use for quantitating nucleic acid by absorbance of light?
What is the best solvent to use for quantitating nucleic acid by absorbance of light?
Why is the absorbance of light at 260nm used for quantitating nucleic acid?
Why is the absorbance of light at 260nm used for quantitating nucleic acid?
What is the main concern when quantitating nucleic acid at 280nm?
What is the main concern when quantitating nucleic acid at 280nm?
What does the genotype of a bacterial strain typically describe?
What does the genotype of a bacterial strain typically describe?
What is the significance of the notation 'galK2' in a bacterial genotype?
What is the significance of the notation 'galK2' in a bacterial genotype?
What is the purpose of quantitating DNA in molecular biology?
What is the purpose of quantitating DNA in molecular biology?
What is the role of restriction enzymes in molecular biology?
What is the role of restriction enzymes in molecular biology?
How is the purity of DNA typically assessed?
How is the purity of DNA typically assessed?
What is the purpose of using bacterial strains like DH5α and SHuffle T7 Express in molecular biology?
What is the purpose of using bacterial strains like DH5α and SHuffle T7 Express in molecular biology?
