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Questions and Answers

Which of the following sequences could represent the DNA after one round of replication in the nonradioactive medium?

  • A A T T G A T C TTAACTAG
  • A* A* T T G A* T C T T A* A* C T A* G
  • A* A* T T G A* T C T T AACT AG
  • A* A T T G A* T C T T A* A* C T A G (correct)

What joins DNA fragments to the lagging strand?

  • Telomere
  • Centromere
  • Helicase
  • DNA Ligase (correct)

What is the function of 3'-5' exonuclease activity in DNA polymerase III?

  • Proof-reading of RNA primers
  • Proof-reading of the nascent DNA (correct)
  • DNA break repair
  • Removal of Okazaki fragments

What molecules are key to passing certain types of chromatin structure to daughter cells during cell reproduction?

<p>Histone molecules (C)</p> Signup and view all the answers

What is the function of telomeres?

<p>A and C only (C)</p> Signup and view all the answers

What regulates the initiation of Eukaryotic DNA replication?

<p>Phosphorylation of ORC and MCM helicase (B)</p> Signup and view all the answers

When does DNA replication occur?

<p>S phase (D)</p> Signup and view all the answers

Which of the following is NOT involved in DNA replication?

<p>DNA polymerase I (C)</p> Signup and view all the answers

What is the function of DNA helicase during DNA replication?

<p>Unwinds the double-stranded DNA (A)</p> Signup and view all the answers

What is the role of DNA ligase in DNA replication?

<p>Joins Okazaki fragments (B)</p> Signup and view all the answers

Flashcards

Proof-reading in DNA replication

DNA polymerase III removes incorrectly paired nucleotides from the newly synthesized strand by digesting it in the 3'-5' direction, increasing accuracy.

Function of DNA polymerase I

DNA polymerase I removes RNA primers and fills in the gaps between Okazaki fragments on the lagging strand.

DNA ligase's role in DNA replication

The enzyme DNA ligase joins the ends of DNA fragments together to create a continuous DNA strand.

Telomeres

A short, repetitive DNA sequence that is found at the ends of chromosomes. Telomeres protect the ends of chromosomes from degradation and prevent fusion with other chromosomes.

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When does DNA replication occur?

Replication of DNA occurs during the S phase of the cell cycle.

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Initiation of Eukaryotic DNA replication

The Origin Recognition Complex (ORC) binds to specific DNA sequences and recruits other proteins to initiate DNA replication.

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Function of DNA helicase

DNA helicase unwinds the double helix of DNA, separating the two strands.

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Origin Recognition Complex (ORC)

A complex of proteins that bind to the origin of replication, recruiting other proteins to initiate replication.

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DNA polymerase III

DNA polymerase III is the primary enzyme responsible for synthesizing new DNA strands during replication.

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Okazaki Fragments

Short, newly synthesized DNA fragments that are joined together by DNA ligase to form the lagging strand.

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