Bacterial Culture Plate Count Quiz

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10 Questions

How many colonies will plate E have?

34

What is the volume of the original culture?

1 ml

What is the volume of the culture in plate A?

1 ml

What is the volume of the culture in plate B?

1 ml

What is the volume of the culture in plate F?

0.1 ml

What is the total volume of diluted culture used for inoculating plates A, B, D, and F?

11 ml

What is the dilution factor for the culture used to inoculate plate E?

1:1000

If the original culture had a concentration of $10^6$ cells/ml, what is the approximate concentration of cells in the culture used to inoculate plate E?

$10^3$ cells/ml

If the original culture had a concentration of $10^6$ cells/ml, what is the approximate number of cells inoculated onto plate E?

3400

Which of the following plates is expected to have the highest number of colonies?

Plate F

Study Notes

Plate E Colony Count

  • Original culture: 1ml
  • Volume transferred to Plate E: 0.1ml
  • Total volume of Plate E: 100ml
  • To find the number of colonies on Plate E, calculate the dilution factor

Test your knowledge on determining the number of colonies in a bacterial culture plate. Given the initial culture volumes and dilutions, calculate the final colony count on plate E.

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