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Questions and Answers
Which staining technique is commonly used to visualize bacteria?
Which staining technique is commonly used to visualize bacteria?
What color do Gram-positive bacteria appear after staining?
What color do Gram-positive bacteria appear after staining?
What color do Gram-negative bacteria appear after staining?
What color do Gram-negative bacteria appear after staining?
What is the main component of the peptidoglycan layer in bacteria?
What is the main component of the peptidoglycan layer in bacteria?
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Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
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What are the two groups that bacteria are classified into based on the Gram stain?
What are the two groups that bacteria are classified into based on the Gram stain?
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What is the function of the peptidoglycan layer in bacteria?
What is the function of the peptidoglycan layer in bacteria?
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What is the composition of the peptidoglycan layer in bacteria?
What is the composition of the peptidoglycan layer in bacteria?
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Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
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Where is the enzyme transpeptidase located in bacteria?
Where is the enzyme transpeptidase located in bacteria?
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Study Notes
Classification of Microorganisms
- Bacteria are classified according to their immunologic and genetic characteristics
Gram Stain
- Gram stain is the most popular means of visualizing bacteria
- It groups bacteria into 2 categories: Gram-Positive bacteria and Gram-Negative bacteria
- Gram stain helps to visualize the shape of bacteria
- The difference in stains is due to differences in the cell wall of bacteria
Gram-Positive Bacteria
- Appear blue under Gram stain
- Have a thicker peptidoglycan layer
Gram-Negative Bacteria
- Appear red under Gram stain
- Have a thinner peptidoglycan layer
Bacterial Cell Structure
- Bacteria have a cytoplasmic membrane that surrounds the cytoplasm
- They also have a peptidoglycan layer (also known as the cell wall) that surrounds the cytoplasmic membrane
- The peptidoglycan layer is composed of repeating disaccharide units with amino acid side chains
- The monomers are held together by the enzyme transpeptidase, located on the inner surface of the cytoplasmic membrane
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Description
Test your knowledge of bacterial classification with this quiz! Learn about the Gram stain and how it helps classify bacteria into Gram-positive and Gram-negative groups based on immunologic and genetic characteristics.
