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Questions and Answers
Which staining technique is commonly used to visualize bacteria?
Which staining technique is commonly used to visualize bacteria?
- Gram stain (correct)
- Ziehl-Neelsen stain
- Acid-fast stain
- Wright stain
What color do Gram-positive bacteria appear after staining?
What color do Gram-positive bacteria appear after staining?
- Blue (correct)
- Yellow
- Purple
- Red
What color do Gram-negative bacteria appear after staining?
What color do Gram-negative bacteria appear after staining?
- Purple
- Yellow
- Red (correct)
- Blue
What is the main component of the peptidoglycan layer in bacteria?
What is the main component of the peptidoglycan layer in bacteria?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
What are the two groups that bacteria are classified into based on the Gram stain?
What are the two groups that bacteria are classified into based on the Gram stain?
What is the function of the peptidoglycan layer in bacteria?
What is the function of the peptidoglycan layer in bacteria?
What is the composition of the peptidoglycan layer in bacteria?
What is the composition of the peptidoglycan layer in bacteria?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
Which enzyme is responsible for holding the monomers in the peptidoglycan layer together?
Where is the enzyme transpeptidase located in bacteria?
Where is the enzyme transpeptidase located in bacteria?
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Study Notes
Classification of Microorganisms
- Bacteria are classified according to their immunologic and genetic characteristics
Gram Stain
- Gram stain is the most popular means of visualizing bacteria
- It groups bacteria into 2 categories: Gram-Positive bacteria and Gram-Negative bacteria
- Gram stain helps to visualize the shape of bacteria
- The difference in stains is due to differences in the cell wall of bacteria
Gram-Positive Bacteria
- Appear blue under Gram stain
- Have a thicker peptidoglycan layer
Gram-Negative Bacteria
- Appear red under Gram stain
- Have a thinner peptidoglycan layer
Bacterial Cell Structure
- Bacteria have a cytoplasmic membrane that surrounds the cytoplasm
- They also have a peptidoglycan layer (also known as the cell wall) that surrounds the cytoplasmic membrane
- The peptidoglycan layer is composed of repeating disaccharide units with amino acid side chains
- The monomers are held together by the enzyme transpeptidase, located on the inner surface of the cytoplasmic membrane
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