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Questions and Answers
What is the primary advantage of using 16S rRNA genes for bacterial classification?
What is the primary advantage of using 16S rRNA genes for bacterial classification?
Which of the following is NOT a component of polyphasic taxonomy?
Which of the following is NOT a component of polyphasic taxonomy?
What distinguishes the classification of a bacterial species from other organisms?
What distinguishes the classification of a bacterial species from other organisms?
Which description accurately defines mutualism?
Which description accurately defines mutualism?
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In the context of bacterial relationships, what term describes an organism living in symbiosis with another dissimilar organism?
In the context of bacterial relationships, what term describes an organism living in symbiosis with another dissimilar organism?
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Which characteristic is associated with the polysaccharide component of bacteria?
Which characteristic is associated with the polysaccharide component of bacteria?
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What is the primary differentiating factor detected by Gram staining?
What is the primary differentiating factor detected by Gram staining?
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What color do Gram-positive bacteria appear after undergoing a Gram-staining procedure?
What color do Gram-positive bacteria appear after undergoing a Gram-staining procedure?
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What is the function of iodine in the Gram-staining procedure?
What is the function of iodine in the Gram-staining procedure?
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What is the role of alcohol in the Gram-staining technique?
What is the role of alcohol in the Gram-staining technique?
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Which component of the mycobacterial cell wall prevents decolorization during acid-fast staining?
Which component of the mycobacterial cell wall prevents decolorization during acid-fast staining?
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Which of the following genera lacks a cell wall?
Which of the following genera lacks a cell wall?
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Which of these processes is a function of the bacterial plasma membrane?
Which of these processes is a function of the bacterial plasma membrane?
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Where is the genetic information to produce endotoxins typically located?
Where is the genetic information to produce endotoxins typically located?
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Which of the following is a characteristic of exotoxins, but not endotoxins?
Which of the following is a characteristic of exotoxins, but not endotoxins?
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Why are exotoxins considered more suitable for vaccine development compared to endotoxins?
Why are exotoxins considered more suitable for vaccine development compared to endotoxins?
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What is the primary mechanism by which superantigens disrupt the host immune system?
What is the primary mechanism by which superantigens disrupt the host immune system?
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Which component of endotoxins is primarily responsible for its virulence?
Which component of endotoxins is primarily responsible for its virulence?
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Why is Listeria monocytogenes described as intracellular?
Why is Listeria monocytogenes described as intracellular?
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Which type of pathogen requires infecting a host to multiply and transmit?
Which type of pathogen requires infecting a host to multiply and transmit?
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What primarily differentiates an opportunistic pathogen from an obligate pathogen?
What primarily differentiates an opportunistic pathogen from an obligate pathogen?
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Which of the following is a key difference between exotoxins and endotoxins regarding their stability??
Which of the following is a key difference between exotoxins and endotoxins regarding their stability??
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Which of the following is true of endotoxins?
Which of the following is true of endotoxins?
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Which of the following environmental factors can influence bacterial pathogenesis?
Which of the following environmental factors can influence bacterial pathogenesis?
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According to the information, what is the definition of pathogenesis?
According to the information, what is the definition of pathogenesis?
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Which factor is most crucial in determining whether an infection results in disease?
Which factor is most crucial in determining whether an infection results in disease?
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Which scenario best describes a facultative pathogen's behavior?
Which scenario best describes a facultative pathogen's behavior?
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What is a disease, in the context of infectious agents?
What is a disease, in the context of infectious agents?
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Which of the following is NOT explicitly listed as influencing bacterial pathogenesis?
Which of the following is NOT explicitly listed as influencing bacterial pathogenesis?
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What mechanism involves the transfer of virulent gene factors via a viral vector?
What mechanism involves the transfer of virulent gene factors via a viral vector?
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Which of the following is NOT considered a virulence factor?
Which of the following is NOT considered a virulence factor?
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What process allows bacteria to temporarily make direct contact to transfer genetic material?
What process allows bacteria to temporarily make direct contact to transfer genetic material?
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In terms of group functioning, what is the role of autoinducers in bacteria?
In terms of group functioning, what is the role of autoinducers in bacteria?
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Which structural component aids in the motility of spirochetes like those causing leptospirosis?
Which structural component aids in the motility of spirochetes like those causing leptospirosis?
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What characteristic best describes quorum sensing in bacteria?
What characteristic best describes quorum sensing in bacteria?
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Which of the following mechanisms does NOT involve transfer of genetic material?
Which of the following mechanisms does NOT involve transfer of genetic material?
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What can be inferred about the importance of biofilm formation in bacteria?
What can be inferred about the importance of biofilm formation in bacteria?
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Which type of bacteria possesses a thick peptidoglycan layer with (lipo)teichoic acids?
Which type of bacteria possesses a thick peptidoglycan layer with (lipo)teichoic acids?
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What structure is characteristic of Gram-negative bacteria?
What structure is characteristic of Gram-negative bacteria?
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Which statement is true regarding the nucleoid in bacterial cells?
Which statement is true regarding the nucleoid in bacterial cells?
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Which bacterial structure is primarily responsible for evading phagocytosis?
Which bacterial structure is primarily responsible for evading phagocytosis?
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Which type of bacteria utilizes Ziehl-Neelsen (acid-base) staining?
Which type of bacteria utilizes Ziehl-Neelsen (acid-base) staining?
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During which phase do cells experience a balance between death and slow multiplication?
During which phase do cells experience a balance between death and slow multiplication?
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Which bacterial structure would one expect in a cell known for resistance to environmental stress?
Which bacterial structure would one expect in a cell known for resistance to environmental stress?
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Which of the following is NOT true for Gram-negative bacteria?
Which of the following is NOT true for Gram-negative bacteria?
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Which of these characteristics is exclusive to eukaryotic cells compared to prokaryotic cells?
Which of these characteristics is exclusive to eukaryotic cells compared to prokaryotic cells?
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What is the primary function of the bacterial cell wall?
What is the primary function of the bacterial cell wall?
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What is the key structural component of the bacterial cell wall that provides rigidity?
What is the key structural component of the bacterial cell wall that provides rigidity?
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Which of the following is true regarding bacterial reproduction?
Which of the following is true regarding bacterial reproduction?
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Which bacterial genus is an exception to the rule that bacteria have a cell wall?
Which bacterial genus is an exception to the rule that bacteria have a cell wall?
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What component is exclusively found in prokaryotic cell walls, providing rigidity?
What component is exclusively found in prokaryotic cell walls, providing rigidity?
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Which of the following characteristics is associated with Gram-positive bacteria?
Which of the following characteristics is associated with Gram-positive bacteria?
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Which component of Gram-negative bacteria acts as an endotoxin that can trigger an immune response?
Which component of Gram-negative bacteria acts as an endotoxin that can trigger an immune response?
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What role do teichoic and lipoteichoic acids play in Gram-positive bacteria?
What role do teichoic and lipoteichoic acids play in Gram-positive bacteria?
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How does the cell wall structure of Gram-negative bacteria contribute to their properties?
How does the cell wall structure of Gram-negative bacteria contribute to their properties?
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What is the primary basis for classifying organisms using 16S rRNA genes?
What is the primary basis for classifying organisms using 16S rRNA genes?
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Which of the following statements accurately describes the role of beneficial bacteria?
Which of the following statements accurately describes the role of beneficial bacteria?
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What criteria are used to classify bacteria using polyphasic taxonomy?
What criteria are used to classify bacteria using polyphasic taxonomy?
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In a mutualistic symbiotic relationship, which of the following is true?
In a mutualistic symbiotic relationship, which of the following is true?
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What is the significance of the LPSN (List of Prokaryotic names with Standing in Nomenclature) in bacterial classification?
What is the significance of the LPSN (List of Prokaryotic names with Standing in Nomenclature) in bacterial classification?
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Which bacterial shape is characterized by a flexible, spiral form?
Which bacterial shape is characterized by a flexible, spiral form?
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What process describes bacterial reproduction where a cell divides into two identical daughter cells?
What process describes bacterial reproduction where a cell divides into two identical daughter cells?
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Which term defines the time required for a bacterial cell to divide into two daughter cells?
Which term defines the time required for a bacterial cell to divide into two daughter cells?
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During which phase of the bacterial growth curve does cell metabolism remain highly active while cell division is absent?
During which phase of the bacterial growth curve does cell metabolism remain highly active while cell division is absent?
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In which phase of the bacterial growth curve does the rate of multiplication equal the rate of cell death?
In which phase of the bacterial growth curve does the rate of multiplication equal the rate of cell death?
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What is the term for bacteria that thrive in the absence of oxygen?
What is the term for bacteria that thrive in the absence of oxygen?
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Which of the following describes bacteria that require lower than atmospheric levels of oxygen for optimal growth?
Which of the following describes bacteria that require lower than atmospheric levels of oxygen for optimal growth?
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What is the term for bacteria that require carbon dioxide for optimal growth?
What is the term for bacteria that require carbon dioxide for optimal growth?
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What is the optimal pH range for the growth of most pathogenic bacteria?
What is the optimal pH range for the growth of most pathogenic bacteria?
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What is the term for an organism that can grow under both aerobic and anaerobic conditions?
What is the term for an organism that can grow under both aerobic and anaerobic conditions?
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Which of the following accurately describes the immediate step following a pathogen's entry into a host during infection?
Which of the following accurately describes the immediate step following a pathogen's entry into a host during infection?
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Under what condition would an infection definitively NOT lead to the host becoming a carrier of the disease?
Under what condition would an infection definitively NOT lead to the host becoming a carrier of the disease?
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During which phase of infection is the bacteria actively multiplying within the host, yet the host is not yet capable of transmitting the infection to others?
During which phase of infection is the bacteria actively multiplying within the host, yet the host is not yet capable of transmitting the infection to others?
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At what point during the course of an infectious disease do the initial symptoms experienced by the patient typically manifest?
At what point during the course of an infectious disease do the initial symptoms experienced by the patient typically manifest?
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A patient presents with symptoms including multiple-organ system failure, shock, and a high fever. Which type of virulence factor is most likely responsible for these severe systemic effects?
A patient presents with symptoms including multiple-organ system failure, shock, and a high fever. Which type of virulence factor is most likely responsible for these severe systemic effects?
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A patient presents with multiple-organ system failure, shock, and fever. Which virulence factor is most likely responsible for these symptoms?
A patient presents with multiple-organ system failure, shock, and fever. Which virulence factor is most likely responsible for these symptoms?
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Virulent gene factors are transferred to other bacteria via a viral vector. Which mechanism describes this process?
Virulent gene factors are transferred to other bacteria via a viral vector. Which mechanism describes this process?
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What process is described when a dense bacterial population releases autoinducers, leading to group functioning?
What process is described when a dense bacterial population releases autoinducers, leading to group functioning?
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Which of the following is an accurate statement regarding peptidoglycan?
Which of the following is an accurate statement regarding peptidoglycan?
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In a bacterial population, what condition is required for autoinducers to effectively trigger group functioning?
In a bacterial population, what condition is required for autoinducers to effectively trigger group functioning?
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Flashcards
Polyphasic taxonomy
Polyphasic taxonomy
Classification of bacteria based on genotypic, phylogenetic, and phenotypic properties.
16s rRNA genes
16s rRNA genes
Highly conserved genes used for accurate classification of organisms.
Beneficial bacteria
Beneficial bacteria
Non-harmful bacteria that can aid in various life processes.
Symbiont
Symbiont
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Mutualism
Mutualism
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Cell Disruption
Cell Disruption
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Polysaccharide Antigenicity
Polysaccharide Antigenicity
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Gram-positive Bacteria
Gram-positive Bacteria
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Gram-negative Bacteria
Gram-negative Bacteria
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Mycobacteria Cell Wall
Mycobacteria Cell Wall
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Mycoplasma
Mycoplasma
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Plasma Membrane Composition
Plasma Membrane Composition
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Plasma Membrane Functions
Plasma Membrane Functions
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Pathogen
Pathogen
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Obligate pathogen
Obligate pathogen
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Facultative pathogen
Facultative pathogen
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Opportunistic pathogen
Opportunistic pathogen
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Accidental pathogen
Accidental pathogen
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Infectious disease
Infectious disease
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Pathogenesis
Pathogenesis
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Commensalism
Commensalism
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Listeria
Listeria
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Exotoxin
Exotoxin
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Endotoxin
Endotoxin
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Heat stability
Heat stability
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Antigenicity
Antigenicity
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Vaccine creation
Vaccine creation
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Superantigen
Superantigen
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Plasmid genes
Plasmid genes
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Capsule
Capsule
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Biofilm formation
Biofilm formation
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Quorum sensing
Quorum sensing
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Bacterial conjugation
Bacterial conjugation
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Bacterial transduction
Bacterial transduction
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Virulence factors
Virulence factors
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Nucleoid
Nucleoid
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Zeihl-Neelsen staining
Zeihl-Neelsen staining
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Maximal stationary phase
Maximal stationary phase
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Endospore
Endospore
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Prokaryotes
Prokaryotes
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Eukaryotes
Eukaryotes
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Bacterial Cell Wall
Bacterial Cell Wall
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Peptidoglycan
Peptidoglycan
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Gram-positive Cell Wall
Gram-positive Cell Wall
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Teichoic Acids
Teichoic Acids
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Lipopolysaccharides (LPS)
Lipopolysaccharides (LPS)
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16s rRNA gene sequencing
16s rRNA gene sequencing
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Metabolism and physiology
Metabolism and physiology
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Free-living bacteria
Free-living bacteria
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Step after entering host
Step after entering host
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Infection and carriers
Infection and carriers
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Bacterial multiplication phase
Bacterial multiplication phase
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Onset of symptoms phase
Onset of symptoms phase
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Virulence factor indication
Virulence factor indication
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Corkscrew bacteria
Corkscrew bacteria
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Filamentous bacteria
Filamentous bacteria
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Spirochete
Spirochete
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Bacterial growth curve
Bacterial growth curve
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Generation time
Generation time
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Obligate anaerobes
Obligate anaerobes
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Facultative anaerobes
Facultative anaerobes
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Aerotolerant bacteria
Aerotolerant bacteria
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Lag phase
Lag phase
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Exponential phase
Exponential phase
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Study Notes
Introduction to Microbiology
- Instructor: Justin Hedlund
- Semester: Fall 2023
- Course: Vet Prep RUSVM
- Email: [email protected]
Learning Objectives (VPRP 0906 - Bacteriology - Dr. Gutierrez)
-
Overview of basic bacteriology principles, including cell structure, terminology, virulence factors, taxonomy, and pathogenesis.
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Brief overview of emerging and zoonotic diseases, diagnosis, treatment, and prevention.
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Key characteristics of prokaryotes: Differences with eukaryotes, bacterial cell wall, plasma membrane, nucleoid, flagella, pili/fimbriae, capsule, endospores, Gram staining procedure, and bacterial morphologies.
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Factors for bacterial growth & reproduction (4 phases of the bacterial growth curve).
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Bacteria-specific terminology: symbiotic relationships, pathogen types (obligate, facultative, opportunistic, accidental), infection, inflammation, disease, pathogenesis, pathogenicity, virulence, plasmids, bacteriophages, quorum sensing, aerobic/anaerobic classifications, and culture/antimicrobial media.
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Bacterial pathogenesis: Sequence of events, virulence factors, toxin characteristics (exotoxins, endotoxins), biofilm formation, and virulence factor transfer (conjugation, transduction, transformation).
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Diagnosis of bacterial infections: Direct microscopic observation, cultural and biochemical characteristics, serotyping, molecular techniques, detection of host immune response (serology, cell-mediated immunity).
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Treatment of bacterial diseases: Limiting microbial growth methods, sterilization, antimicrobial classifications (chemical structures, mode of action, spectrum of activity, antimicrobial susceptibility testing).
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Pathogenic bacteria: Specific species (Gram-positive cocci, rods, anaerobes, Gram-negative rods, spirochetes, mycoplasma, rickettsiales) and diseases they cause.
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Bacterial Vaccines: mechanism of action, types, composition, features, and potential vaccination failure.
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Prokaryotes vs. Eukaryotes: Key differences in structure, chromosomes, cytoskeleton, division, cell wall composition, reproduction.
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Bacterial Cell Wall: Functions (protection, transport), differences between structures of various species for pathogenicity, staining properties, peptidoglycan composition (NAG, NAM subunits).
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Gram-positive Bacteria Cell Wall: Multilayered, thick peptidoglycan, resistance to drying, susceptible to ionic detergents, teichoic acids (linked with peptidoglycan and plasma membrane), strong antigenicity for immune responses.
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Gram-negative Bacteria Cell Wall: Thin peptidoglycan layer, outer and inner plasma membranes with a periplasmic space, not resistant to drying/physical disruption, less susceptible to ionic detergents, lipopolysaccharides (LPS), known as endotoxin.
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Gram-staining Technique: How it differentiates bacteria by their physical/chemical properties based upon peptidoglycan composition.
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Mycobacteria Cell Wall: Thick waxy hydrophobic cell wall, mycolic acids, acid-fast staining (Zeihl-Neelsen staining) to visualize it under microscopy.
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Exception: Mycoplasma (lacks cell wall, sterols for plasma membrane rigidity).
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Plasma Membrane: Phospholipids and proteins (no sterols), active transport of nutrients, elimination of metabolites, bacterial respiration, biosynthesis of DNA, cell wall polymers, and lipids. Additional transport systems (Facilitated diffusion, Active transport, Group translocation) and mesosomes.
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Genetic Material Locations: Nucleoid (region where nuclear material is housed, single haploid circular chromosome, double stranded DNA). Plasmids (small DNA that replicate independently, carry virulence factors and resistance genes).
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Flagella: Locomotion structure (filament, hook, basal body), arrangement (monotrichous, amphitrichous, lophotrichous, peritrichous, endoflagella) and function.
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Pili/Fimbriae: Fine hair-like appendages, common in Gram-negative bacteria, composed of pilin protein, host tissue adherence, and antigenicity roles (e.g., E. coli K88 & K99 in neonatal piglets & calves).
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Capsule: Glycocalyx (extracellular polymeric matrix, primarily polysaccharides), nutrient reserve, protection, adherence to host cell surfaces, interferes with phagocytosis.
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Endospores: Survival mechanism (cryptobiotic state of dormancy), durable cell form, ensures survival during adverse conditions.
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Endospores (Sporulation): detailed steps of sporulation from the formation of the forespore with a second membrane, disintegration of the mother chromosomes, and formation of the spore coat to form a mature cell.
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Bacterial Morphology: Details of cell shapes (cocci, streptococci, diplococci, encapsulated diplococci, staphylococci, tetrad, bacillus, diplobacilli, pallisades, streptobacilli, corkscrew, filamentous, spirochete, club rod, helical form, budding/appendaged bacteria). Ability to identify these in microscopy.
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Bacterial Metabolism, Phylogeny, Pathogenesis.
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Factors Required for Bacterial Growth: Gaseous requirements (O2, CO2, aerobic, anaerobic, facultative, microaerophilic, capnophilic), environmental factors (nutrients, pH, ion strength, osmotic pressure, light, temperature).
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Bacterial Reproduction: Binary fission (chromosome replication, cell elongation, and division into two daughter cells). Generation time (time required for bacterial cell division).
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Bacterial Growth Curve: Four phases (Lag, exponential, maximal stationary, decline/death) and associated characteristics (cell size, metabolism, multiplication rate).
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Bacterial Growth Factors: Nutrients (e.g., glucose, nitrates, fatty acids), pH, ionic strength/osmotic pressure, temperature, gaseous requirements (e.g., oxygen, carbon dioxide).
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Methods for Bacterial Counting: Direct smear, counting chamber, spread plate method, pour plate method.
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Phylogeny & Taxonomy: Classification methods (morphology, motility, DNA profiling, metabolism/physiology, biochemical characteristics, 16S rRNA gene sequencing, whole genome sequencing) for polyphasic taxonomy
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We Live in a Microbial World: Types of bacteria (beneficial: Symbionts, commensals, mutualism, free-living), (harmful: obligate, facultative, opportunistic, accidental pathogens).
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Pathogens: Infectious agent, types (obligate, facultative, opportunistic), role in disease.
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Bacterial Pathogenesis: factors impacting disease (host, pathogen, environment) and types of interactions (initial host infection, adherence to epithelial cells, toxin release, invasion/destruction of cells, tissue invasion, systemic infection). Inflammation (local tissue damage, toxins and/or immune response, toxemia, acute/chronic disease).
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Iceberg concept of disease- Majority infections are asymptomatic, others are mild, and a limited number lead to severe illness or death.
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Pathogen Consequences: No colonization, subclinical disease, disease/recovery, disease/persistence, disease/death or disability.
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Time Periods, Symptoms, & Infectiousness: Latent period, incubation period, onset of infectiousness, onset of symptoms, resolution, infectious period.
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Bacterial Virulence Factors: Bacterial traits allowing for pathogenicity (Extracellular, intracellular) including examples of facultative and obligate intracellular pathogens. Discussion of specific virulence factors (toxins, capsules, flagella, fimbriae, etc.).
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Toxin Types: Exotoxins & Endotoxins, properties and how they affect the host. Superantigens- effects of immune system.
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Quorum Sensing: Chemical signals among bacteria for group behavior regulation (e.g., biofilm formation).
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Biofilm Formation: Process from attachment that allows for the spread and resistance to environmental stress.
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Transfer of Virulence Factors: Methods (transformation, transduction, conjugation) and implications.
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Antimicrobial Resistance: Innate and acquired resistance, clinical use of antibiotics.
Additional Information
- Various figures and diagrams accompany the course material(s) to illustrate and explain the concepts mentioned above.
- Quizzes or practice questions were added as part of the slides during the course.
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Description
Test your knowledge on bacterial classification and taxonomy with this quiz. You will explore the characteristics, staining techniques, and symbiotic relationships of bacteria. Perfect for students studying microbiology or related fields.