Podcast
Questions and Answers
What is one of the reasons why vector DNA cannot re-ligate to itself?
What is one of the reasons why vector DNA cannot re-ligate to itself?
- Blunt ends of the digested vector DNA are incompatible (correct)
- Excessive amount of DNA Ligase used
- The size of the vector DNA is too small
- Presence of cohesive ends on the digested vector DNA
Which components are required for making recombinant DNA molecules?
Which components are required for making recombinant DNA molecules?
- DNA Ligase, E.coli, Antibiotic selection
- Plasmid vector, Insert DNA, Restriction enzyme (correct)
- Insert DNA, DNA Ligase, Transformation
- Insert DNA, E.coli, Restriction enzyme
Why is directional cloning made possible with insert DNA fragments?
Why is directional cloning made possible with insert DNA fragments?
- They possess two different cohesive/sticky ends on either side (correct)
- They lack cohesive ends
- They have blunt ends
- They are very large in size
Directional cloning allows the insert to be cloned in which specific orientation?
Directional cloning allows the insert to be cloned in which specific orientation?
How can you determine if cloning has been successful in transformation?
How can you determine if cloning has been successful in transformation?
What must be done to cells to allow them to take up DNA in the process of transformation?
What must be done to cells to allow them to take up DNA in the process of transformation?
In constructing a cDNA library, what is the starting material used?
In constructing a cDNA library, what is the starting material used?
What is the function of genomic libraries?
What is the function of genomic libraries?
How do you verify that a plasmid is truly recombinant?
How do you verify that a plasmid is truly recombinant?
What method is primarily used for generating transgenic bacteria?
What method is primarily used for generating transgenic bacteria?
To clone mRNA sequences into a vector, what type of cDNA copy must be synthesized first?
To clone mRNA sequences into a vector, what type of cDNA copy must be synthesized first?
What is the main purpose of ligating cDNA into a vector in the construction of cDNA libraries?
What is the main purpose of ligating cDNA into a vector in the construction of cDNA libraries?
What is the purpose of using two different restriction enzymes in directional cloning?
What is the purpose of using two different restriction enzymes in directional cloning?
What are cohesive compatible ends in DNA cloning?
What are cohesive compatible ends in DNA cloning?
How does directional cloning ensure specific orientation of insert DNA in the vector?
How does directional cloning ensure specific orientation of insert DNA in the vector?
What is one advantage of using directional cloning in DNA experiments?
What is one advantage of using directional cloning in DNA experiments?
In DNA cloning, what role do cohesive compatible ends play?
In DNA cloning, what role do cohesive compatible ends play?
What is the significance of using two restriction enzymes with different sequences in directional cloning?
What is the significance of using two restriction enzymes with different sequences in directional cloning?
