Podcast
Questions and Answers
What is the process known as when tissues are digested and broken down by their own enzymes?
What is the process known as when tissues are digested and broken down by their own enzymes?
Which type of tissues experience a more severe autolysis due to being rich in enzymes?
Which type of tissues experience a more severe autolysis due to being rich in enzymes?
Why is autolysis retarded by cold?
Why is autolysis retarded by cold?
What is the advantage of examining fresh tissues over preserved tissues?
What is the advantage of examining fresh tissues over preserved tissues?
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Why are preserved tissues preferred for a more effective study as opposed to fresh tissues?
Why are preserved tissues preferred for a more effective study as opposed to fresh tissues?
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Why are liver, brain, and kidney tissues more prone to rapid autolysis compared to elastic and collagen tissues?
Why are liver, brain, and kidney tissues more prone to rapid autolysis compared to elastic and collagen tissues?
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Which method uses microwave radiation to accelerate the fixation process?
Which method uses microwave radiation to accelerate the fixation process?
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What is the purpose of permeabilization with 0.5% Triton X-100?
What is the purpose of permeabilization with 0.5% Triton X-100?
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Which fixation method involves fixing tissue in a solution containing paraformaldehyde and methanol?
Which fixation method involves fixing tissue in a solution containing paraformaldehyde and methanol?
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What is the purpose of decalcification in tissue processing?
What is the purpose of decalcification in tissue processing?
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What should be optimized for consistent results in microwave fixation?
What should be optimized for consistent results in microwave fixation?
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What is the primary step after the fixation process with tissues according to the text?
What is the primary step after the fixation process with tissues according to the text?
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What is the purpose of decalcification in tissue processing?
What is the purpose of decalcification in tissue processing?
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When should decalcification be performed in the tissue processing workflow?
When should decalcification be performed in the tissue processing workflow?
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What is a consequence of inadequate decalcification in tissue processing?
What is a consequence of inadequate decalcification in tissue processing?
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Which factor does NOT influence the rate of decalcification?
Which factor does NOT influence the rate of decalcification?
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What is the purpose of surface decalcification in tissue processing?
What is the purpose of surface decalcification in tissue processing?
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Which type of decalcifying agents are based on chelating agents?
Which type of decalcifying agents are based on chelating agents?
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What may be experienced when sectioning a paraffin-embedded block with small foci of calcification on the tissue surface?
What may be experienced when sectioning a paraffin-embedded block with small foci of calcification on the tissue surface?
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What is recommended for unduly hard tissues that may damage microtome knives?
What is recommended for unduly hard tissues that may damage microtome knives?
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Which substance can act as both a decalcifying agent and a tissue softener?
Which substance can act as both a decalcifying agent and a tissue softener?
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How long should selected portions of unduly hard tissues be left in Perenyi's fluid to facilitate softening?
How long should selected portions of unduly hard tissues be left in Perenyi's fluid to facilitate softening?
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What effect does immersion in 4% aqueous phenol solution have on fixed tissues?
What effect does immersion in 4% aqueous phenol solution have on fixed tissues?
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What impact does submerging the cut surface of a block in Perenyi's fluid have before sectioning?
What impact does submerging the cut surface of a block in Perenyi's fluid have before sectioning?
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What is the process by which impregnated tissue is placed into a mold containing a medium that solidifies?
What is the process by which impregnated tissue is placed into a mold containing a medium that solidifies?
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Which of the following is NOT an ideal characteristic of an infiltrating and embedding medium?
Which of the following is NOT an ideal characteristic of an infiltrating and embedding medium?
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What is the general term for the medium used to infiltrate the tissue after impregnation?
What is the general term for the medium used to infiltrate the tissue after impregnation?
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Which of the following is NOT one of the four types of impregnation and embedding medium mentioned in the text?
Which of the following is NOT one of the four types of impregnation and embedding medium mentioned in the text?
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If the embedding medium is too soft for the tissue material, what issue may occur during sectioning?
If the embedding medium is too soft for the tissue material, what issue may occur during sectioning?
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Why is it important for the embedding medium to match the tissue type in strength and hardness?
Why is it important for the embedding medium to match the tissue type in strength and hardness?
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