30 Questions
What is the process known as when tissues are digested and broken down by their own enzymes?
Autolysis
Which type of tissues experience a more severe autolysis due to being rich in enzymes?
Liver, brain, and kidney tissues
Why is autolysis retarded by cold?
Cold inhibits enzyme activity
What is the advantage of examining fresh tissues over preserved tissues?
Allows observation of living protoplasmic activities
Why are preserved tissues preferred for a more effective study as opposed to fresh tissues?
Allows long-term storage and re-examination
Why are liver, brain, and kidney tissues more prone to rapid autolysis compared to elastic and collagen tissues?
Rich in enzymes
Which method uses microwave radiation to accelerate the fixation process?
Microwave Fixation
What is the purpose of permeabilization with 0.5% Triton X-100?
Enhancing membrane permeability
Which fixation method involves fixing tissue in a solution containing paraformaldehyde and methanol?
Paraformaldehyde-methanol fixation
What is the purpose of decalcification in tissue processing?
Removing calcium deposits
What should be optimized for consistent results in microwave fixation?
Microwave parameters
What is the primary step after the fixation process with tissues according to the text?
Processing
What is the purpose of decalcification in tissue processing?
To remove calcium or lime salts from tissues
When should decalcification be performed in the tissue processing workflow?
After fixation and before impregnation
What is a consequence of inadequate decalcification in tissue processing?
Poor cutting of hard tissues
Which factor does NOT influence the rate of decalcification?
Time of day
What is the purpose of surface decalcification in tissue processing?
To deal with small unexpected deposits of calcium in paraffin blocks
Which type of decalcifying agents are based on chelating agents?
Those composed of chelating agents themselves
What may be experienced when sectioning a paraffin-embedded block with small foci of calcification on the tissue surface?
Resistance or a 'grating' sensation
What is recommended for unduly hard tissues that may damage microtome knives?
Usage of tissue softeners
Which substance can act as both a decalcifying agent and a tissue softener?
Perenyi's fluid
How long should selected portions of unduly hard tissues be left in Perenyi's fluid to facilitate softening?
12-24 hours
What effect does immersion in 4% aqueous phenol solution have on fixed tissues?
Causes tissue softening and easier sectioning
What impact does submerging the cut surface of a block in Perenyi's fluid have before sectioning?
Facilitates easier cutting of tissues
What is the process by which impregnated tissue is placed into a mold containing a medium that solidifies?
Embedding
Which of the following is NOT an ideal characteristic of an infiltrating and embedding medium?
Colored
What is the general term for the medium used to infiltrate the tissue after impregnation?
Embedding Medium
Which of the following is NOT one of the four types of impregnation and embedding medium mentioned in the text?
Starch
If the embedding medium is too soft for the tissue material, what issue may occur during sectioning?
Sections will be torn or shredded
Why is it important for the embedding medium to match the tissue type in strength and hardness?
To avoid tearing or shredding of sections
Test your knowledge on autolysis, the process where tissues are digested by their own enzymes once removed from the body. Explore methods of tissue examination and understand how the process varies across different types of tissues.
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