MIC exam
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Questions and Answers

What are the very small units that matter is composed of?

Atoms

Atoms are composed of which three 'subatomic particles'?

  • Protons, neutrons, and electrons (correct)
  • Ions, Protons and Electrons
  • Cations, Neutrons and Electrons

Protons and neutrons are around the outside of the nucleus and electrons are in the center?

False (B)

What is formed when an atom's electrons are involved in combining the atom with other atoms?

<p>Chemical bonds</p> Signup and view all the answers

Atoms are most stable when their outermost what are completely filled?

<p>Energy shells</p> Signup and view all the answers

What is the name of a positively-charged atoms?

<p>Cations</p> Signup and view all the answers

What kind of bonds are formed when cations and anions attract each other?

<p>Ionic bonds</p> Signup and view all the answers

What are compounds typically form?

<p>Crystalline compounds</p> Signup and view all the answers

Some atoms prefer to exchange electrons with other atoms rather than share them?

<p>False (B)</p> Signup and view all the answers

What is the relative attraction of an atom for its electrons called?

<p>Electronegativity</p> Signup and view all the answers

What is formed by the joining of two or more atoms with covalent bonds?

<p>Molecule</p> Signup and view all the answers

Shared electrons spend an equal amount of time around each nucleus. What kind of bonds are these?

<p>Nonpolar covalent bonds</p> Signup and view all the answers

Unequal sharing of electrons due to significantly different electronegativities refers to what kind of bonds?

<p>Polar covalent bonds</p> Signup and view all the answers

What kind of attractions exist between opposite partial charges of molecules exhibiting polar covalent bonds?

<p>Hydrogen bonds</p> Signup and view all the answers

What involves reactants and products?

<p>Chemical reactions</p> Signup and view all the answers

What are the three categories of chemical reactions?

<p>Synthesis, decomposition and exchange reactions (B)</p> Signup and view all the answers

Cells make ATP by first releasing energy in the form of what, from organic and inorganic compounds (electron donors)?

<p>Electrons</p> Signup and view all the answers

Reduction involves loss of electrons?

<p>False (B)</p> Signup and view all the answers

What are large, carbon-containing molecules?

<p>Organic Macromolecules</p> Signup and view all the answers

What are the four main classes of organic macromolecules?

<p>Lipids, carbohydrates, proteins, and nucleic acids</p> Signup and view all the answers

What is composed of fatty acid monomers that contain mostly carbon and hydrogen?

<p>Lipids</p> Signup and view all the answers

Which of these is NOT a group of lipids?

<p>Carbohydrates (C)</p> Signup and view all the answers

What contains one long-chain fatty acid covalently linked to a long-chain alcohol by an ester bond?

<p>Waxes</p> Signup and view all the answers

What are complex hydrocarbons based upon a sterol ring?

<p>Steroids</p> Signup and view all the answers

What organic molecules are composed of carbon, hydrogen, and oxygen $(CH_2O)_n$?

<p>Carbohydrates</p> Signup and view all the answers

Given the role of microorganisms, what far-reaching consequence would their complete absence have on Earth's ecosystems?

<p>Drastic alterations in nutrient cycles and the accumulation of undecomposed organic matter. (B)</p> Signup and view all the answers

Considering the classification of microorganisms, how does a virus like Ebola differ fundamentally from bacteria or fungi regarding its replication?

<p>Viruses require a host cell to replicate, utilizing the host's cellular machinery. (D)</p> Signup and view all the answers

If all microorganisms suddenly vanished, how could this affect the global carbon cycle, and what would be the most likely outcome?

<p>The carbon cycle would slow down, leading to an accumulation of carbon in organic matter. (B)</p> Signup and view all the answers

What would most likely happen to the levels of atmospheric oxygen ($O_2$) if all photosynthetic microorganisms disappeared?

<p>Atmospheric $O_2$ levels would decrease over time as oxygen-consuming processes continue without replacement from microbial photosynthesis. (D)</p> Signup and view all the answers

Considering the ecological roles of microorganisms, what would be the most direct consequence of their absence on the availability of nutrients for plant growth?

<p>Nutrient availability would decrease due to the reduced decomposition of organic matter and nutrient cycling. (D)</p> Signup and view all the answers

What is a key characteristic that distinguishes Archaea from other types of microorganisms?

<p>Archaea possess cell walls made of polymers other than peptidoglycan. (B)</p> Signup and view all the answers

Which of the following best describes the relationship between early human societies and microorganisms before the advent of microbiology?

<p>Early humans unknowingly interacted with microorganisms through food production and disease. (D)</p> Signup and view all the answers

What key development led to the establishment of sedentary societies in human history?

<p>The domestication of plants and livestock. (C)</p> Signup and view all the answers

Which of the following best describes the host range of viruses?

<p>Viruses exhibit some specificity, with different viruses targeting animal, plant, or bacterial cells. (C)</p> Signup and view all the answers

Which of the following characteristics was NOT easily observable when early scientists studied biological organisms?

<p>Metabolism (Ability to extract energy from a molecule) (D)</p> Signup and view all the answers

What technological advancement was crucial in order to measure an organism's response to the environment?

<p>Microscopes (B)</p> Signup and view all the answers

What characteristic distinguishes algae from viruses?

<p>Algae are photosynthetic, while viruses are not. (A)</p> Signup and view all the answers

What does the Theory of Spontaneous Generation attempt to explain?

<p>The reproduction of small organisms (C)</p> Signup and view all the answers

Unlike viruses, algae are capable of:

<p>Carrying out photosynthesis. (C)</p> Signup and view all the answers

Which of the following is a key difference between algae and viruses concerning their cellular structure?

<p>Algae can be either unicellular or multicellular, while viruses are non-cellular entities. (D)</p> Signup and view all the answers

Considering the historical context of microbiology, which factor most likely hindered the immediate acceptance of germ theory over spontaneous generation?

<p>Limited understanding of microscopic life and experimental methodologies. (D)</p> Signup and view all the answers

If a newly discovered microorganism is found thriving in a hot spring with temperatures exceeding 70°C and its cell wall lacks peptidoglycan, to which domain would it likely belong?

<p>Archaea (A)</p> Signup and view all the answers

Consider a newly discovered microorganism that is photosynthetic but not green. Based on the provided information, to which major class does it likely belong?

<p>Algae (D)</p> Signup and view all the answers

A scientist is studying a microbe that infects bacterial cells exclusively. Which type of microbe is the scientist most likely studying?

<p>A bacteriophage (a virus that infects bacteria). (B)</p> Signup and view all the answers

If a new disease is discovered that affects plant cells, which type of microorganism is most likely the cause?

<p>A plant virus (C)</p> Signup and view all the answers

Which of the following characteristics is exclusively associated with viruses?

<p>Requirement of a host cell for replication. (D)</p> Signup and view all the answers

What was the prevailing belief about the origin of smaller life forms, such as flies and fleas, during the Middle Ages?

<p>They spontaneously generated from non-living matter. (D)</p> Signup and view all the answers

Which of the following reflects the influence of the Church and religious leaders on people's understanding of disease causation during the Middle Ages?

<p>Believing diseases were caused by bad spirits. (D)</p> Signup and view all the answers

What advancement did the Romans make that directly contributed to public health?

<p>The development of water pipes and aqueducts. (B)</p> Signup and view all the answers

Why did early civilizations struggle to connect spoiled food and dirty water with the spread of microbes and disease?

<p>Microbes were invisible to the naked eye, which lead to a lack of awareness. (B)</p> Signup and view all the answers

What was Antoni van Leeuwenhoek's primary contribution to the field of microbiology?

<p>Visualizing and describing microorganisms using simple microscopes. (A)</p> Signup and view all the answers

What term did Leeuwenhoek use to refer to the microorganisms he observed?

<p>Animalcules (D)</p> Signup and view all the answers

Why was there a problem with early systems for classifying life?

<p>The system relied on subjective descriptions that varied between individuals. (B)</p> Signup and view all the answers

What is the lasting impact of Leeuwenhoek's work?

<p>The insights from Leeuwenhoek's detailed observations provided an entirely new direction for the field of medicine. (D)</p> Signup and view all the answers

What is a primary requirement for an agent to fulfill Koch's postulates?

<p>The agent must be isolated and cultured outside of the host. (B)</p> Signup and view all the answers

Which of the following scenarios would represent a limitation of Koch's postulates?

<p>A virus that requires a host cell to replicate and cannot be grown in artificial media. (B)</p> Signup and view all the answers

What is the role of a hypothesis in the scientific method?

<p>To provide a potential answer to a question based on observation. (A)</p> Signup and view all the answers

In the scientific method, what is the purpose of conducting an experiment?

<p>To test a hypothesis. (C)</p> Signup and view all the answers

In the context of disease causation, what does 'synergy' refer to, as it relates to limitations of Koch's postulates?

<p>The phenomenon where multiple organisms work together to cause disease. (A)</p> Signup and view all the answers

Which historical figure is credited with introducing cleanliness into the nursing practice?

<p>Florence Nightingale (D)</p> Signup and view all the answers

According to the scientific method, what should a scientist do if the results of an experiment contradict their hypothesis?

<p>Reject or modify the hypothesis. (D)</p> Signup and view all the answers

What contribution to disease prevention is Alexander Fleming best known for?

<p>Discovering antibiotics. (D)</p> Signup and view all the answers

Which of the following is NOT one of Pasteur's contributions?

<p>Development of the first antibiotics. (B)</p> Signup and view all the answers

What is the significance of Pasteur's work on fermentation and diseases of beer and wine?

<p>It led to the development of pasteurization and the understanding that microorganisms can cause disease. (A)</p> Signup and view all the answers

What is the central idea of the germ theory of disease, as developed by Louis Pasteur?

<p>Diseases are caused by microorganisms. (A)</p> Signup and view all the answers

Why is Robert Koch considered the 'Father of the Microbiological Laboratory'?

<p>Because he developed techniques for culturing and studying microorganisms. (B)</p> Signup and view all the answers

Which of Koch's postulates is designed to ensure that the suspected causative agent is truly responsible for the disease?

<p>All of the above (E)</p> Signup and view all the answers

Which type of microscopy is MOST suitable for observing the external surface details of a microorganism with high resolution?

<p>Scanning electron microscopy (SEM) (C)</p> Signup and view all the answers

A researcher needs to visualize the internal structures of a virus with the highest possible resolution. Which type of microscopy is MOST appropriate?

<p>Transmission electron microscopy (TEM) (A)</p> Signup and view all the answers

A scientist is studying the behavior of living cells in a culture and needs to observe them without staining or fixing. Which microscopy technique would be MOST suitable for this purpose, while still providing enhanced contrast?

<p>Dark-field microscopy (B)</p> Signup and view all the answers

When switching from a lower power objective to a higher power objective on a light microscope, what generally happens to the field of view and the amount of light needed for optimal viewing?

<p>The field of view decreases, and more light is needed (C)</p> Signup and view all the answers

In microscopy, what is the relationship between the wavelength of radiation used and the resolving power?

<p>Shorter wavelengths correspond to greater resolving power (A)</p> Signup and view all the answers

How do the plane of cell division and the separation of daughter cells influence bacterial arrangement?

<p>They dictate the clustering and patterns (e.g., chains, clusters) in bacterial populations. (A)</p> Signup and view all the answers

During binary fission in prokaryotes, what is the direct role of the cytoplasmic membrane?

<p>It elongates, separating the replicated DNA molecules. (B)</p> Signup and view all the answers

What is a key characteristic of snapping division that differentiates it from typical binary fission?

<p>Snapping division involves the outer cell wall snapping due to tension from inner wall growth, leading to daughter cells remaining attached. (B)</p> Signup and view all the answers

Which of the following statements accurately describes how arrangements of cocci (e.g., diplococci, streptococci) are formed?

<p>They are directly related to the plane of cell division and whether daughter cells separate. (A)</p> Signup and view all the answers

If a bacterium divides in a single plane and the daughter cells remain attached, what arrangement is most likely to result?

<p>A chain (A)</p> Signup and view all the answers

How does the arrangement of bacterial cells in chains (Streptobacilli) primarily occur?

<p>Arises from cell division in a single plane where daughter cells remain attached. (C)</p> Signup and view all the answers

What cellular process most directly determines the arrangements observed in cocci bacteria such as Staphylococci or Streptococci?

<p>The plane of cell division and cell separation after division. (D)</p> Signup and view all the answers

Which characteristic distinguishes Bacillus and Clostridium species from many other bacterial genera?

<p>Their production of endospores as a survival mechanism. (D)</p> Signup and view all the answers

If a bacterium divides in three planes and the daughter cells remain attached, what arrangement is formed?

<p>Sarcina (A)</p> Signup and view all the answers

What is the primary function of endospores in bacteria such as Bacillus cereus?

<p>To enable survival during unfavorable environmental conditions. (B)</p> Signup and view all the answers

How does immersion oil enhance resolution in bright-field microscopy?

<p>It decreases the amount of light refracted away from the objective lens, capturing more light. (A)</p> Signup and view all the answers

In microscopy, what trade-off often exists between contrast and resolution when adjusting microscope settings?

<p>Increasing contrast may reduce the ability to distinguish fine details. (B)</p> Signup and view all the answers

A microbiologist is preparing a bacterial smear for staining. What is the primary purpose of 'fixing' the smear to the slide?

<p>To kill the bacteria and adhere them to the slide. (B)</p> Signup and view all the answers

If a stain has a positive charge, which type of cellular structure will it most effectively bind to, and what term best describes this type of stain?

<p>Negatively charged structures; basic stain (D)</p> Signup and view all the answers

Suppose you are viewing a specimen under a bright-field microscope with a 10x ocular lens and a 40x objective lens. What is the total magnification of the image you are observing?

<p>400x (A)</p> Signup and view all the answers

Which adjustment to microscope settings would be MOST effective in improving the resolution of a stained bacterial specimen?

<p>Switching to a higher magnification objective lens with a higher numerical apperture (B)</p> Signup and view all the answers

A student is observing a stained bacterial slide under a microscope but struggles to clearly distinguish the bacteria from the background. Besides adjusting the light intensity, what other adjustment to a bright-field microscope would MOST directly improve contrast?

<p>Closing down the condenser aperture diaphragm (D)</p> Signup and view all the answers

Given that light must pass through a medium to illuminate a specimen, how does the medium (e.g., air, water, or oil) primarily affect the quality of the image in microscopy?

<p>By altering the speed and direction of light, which impacts refraction and magnification. (A)</p> Signup and view all the answers

Which type of microscopy involves physical interaction with a pointed probe to visualize samples at the molecular level?

<p>Scanning Probe/Atomic Force Microscopy (AFM) (D)</p> Signup and view all the answers

In the context of staining techniques, what is the primary purpose of a mordant, such as iodine in the Gram stain?

<p>To enhance the binding of the primary stain to the bacterial cell. (C)</p> Signup and view all the answers

A microbiologist observes a bacterial sample that appears purple after Gram staining. Based on this observation, which of the following can be inferred about the bacteria?

<p>It has a thick peptidoglycan layer and is Gram-positive. (D)</p> Signup and view all the answers

Which of the following is NOT a typical application of staining techniques in microscopy?

<p>Determining the three-dimensional structure of macromolecules. (A)</p> Signup and view all the answers

If a bacterial cell's surface is negatively charged, which type of dye would be most effective for staining it?

<p>A basic dye, which carries a positive charge and is attracted. (B)</p> Signup and view all the answers

What is the correct order of steps in the Gram staining procedure?

<p>Crystal violet, iodine, alcohol, safranin (D)</p> Signup and view all the answers

Which microscopic technique would be most appropriate for observing the external surface details of a virus?

<p>Scanning electron microscopy (SEM) (C)</p> Signup and view all the answers

Which of the following characteristics distinguishes differential stains from simple stains?

<p>Differential stains can distinguish between different types of cells or structures, while simple stains only provide contrast. (B)</p> Signup and view all the answers

A dark-field microscope is particularly useful for enhancing contrast in specimens that are:

<p>Transparent or unstained, making them difficult to view with brightfield microscopy. (A)</p> Signup and view all the answers

What is the function of fluorescence microscopy?

<p>To identify and visualize specific components within a cell using fluorescent markers. (A)</p> Signup and view all the answers

What is the primary advantage of confocal microscopy over standard fluorescence microscopy?

<p>Confocal microscopy reduces background noise and allows for the construction of 3D images. (A)</p> Signup and view all the answers

Why are electron microscopes capable of much higher resolution compared to light microscopes?

<p>Electron microscopes use electrons, which have much shorter wavelengths than visible light. (B)</p> Signup and view all the answers

What type of samples are best viewed using electron microscopes, considering their capabilities and limitations?

<p>Thin specimens, like viruses or cellular internal structures, due to the high magnification and resolution. (D)</p> Signup and view all the answers

What is the primary reason electron micrographs are typically presented in black and white, even though the samples themselves may be colored?

<p>The detectors in electron microscopes measure electron density, not color which is why they are grayscale. (D)</p> Signup and view all the answers

How does the preparation of samples typically differ between light microscopy and electron microscopy, considering their illumination sources?

<p>Samples for electron microscopy generally require more extensive processing, including fixation, embedding, sectioning, and staining with heavy metals. (C)</p> Signup and view all the answers

If you need to view the 3D surface structure of a bacterial cell, which type of microscopy would be MOST appropriate?

<p>Scanning electron microscopy (SEM) (B)</p> Signup and view all the answers

According to the information provided, what is the most effective way to contact Patrick Daydif with questions?

<p>In person. (D)</p> Signup and view all the answers

Where can one find the specific office hours for Patrick Daydif?

<p>On Canvas. (B)</p> Signup and view all the answers

Besides the method explicitly stated, what alternative way does Patrick Daydif offer for students to schedule a meeting?

<p>By appointment. (C)</p> Signup and view all the answers

In what context is the information presented most likely to be used?

<p>A university microbiology course. (A)</p> Signup and view all the answers

What is the significance of providing multiple contact methods for Patrick Daydif?

<p>To ensure accessibility and cater to different student preferences. (C)</p> Signup and view all the answers

Which of the following cellular processes is directly facilitated by the cytoskeleton's ability to contract or expand regions of the cell membrane?

<p>Endocytosis and exocytosis. (D)</p> Signup and view all the answers

How does the structure of the nuclear envelope facilitate the transport of large molecules such as proteins into and out of the nucleus?

<p>It contains nuclear pore complexes large enough for macromolecules to pass through. (A)</p> Signup and view all the answers

Considering the differences between eukaryotic and prokaryotic ribosomes, what is the significance of targeting bacterial ribosomes with antibiotics in treating infections?

<p>It selectively inhibits protein synthesis in bacteria without significantly affecting the host's cells. (C)</p> Signup and view all the answers

How do the different protein compositions of microtubules, microfilaments, and intermediate filaments enable the cytoskeleton to perform diverse functions within the cell?

<p>Each protein type provides unique structural properties and functional capabilities. (C)</p> Signup and view all the answers

How does the endoplasmic reticulum (ER) contribute to both protein synthesis and preliminary processing, and what structural feature enables these dual roles?

<p>The ER contains ribosomes on its outer surface for synthesis and enzymes within its lumen for processing. (C)</p> Signup and view all the answers

Which of the following components is unique to the outer membrane of Gram-negative bacteria?

<p>Lipopolysaccharide (LPS) (B)</p> Signup and view all the answers

What color do Gram-negative cells appear after Gram staining, and which dye is responsible for this color?

<p>Pink, due to safranin (B)</p> Signup and view all the answers

Why are many antibiotics designed to inhibit peptidoglycan synthesis?

<p>To weaken the bacterial cell wall, leading to cell lysis (A)</p> Signup and view all the answers

Which model provides the current understanding of cell membrane structure?

<p>The fluid mosaic model (D)</p> Signup and view all the answers

What collective term describes the concentration gradient and electrical gradient maintained by the cell membrane?

<p>Electrochemical gradient (A)</p> Signup and view all the answers

In passive transport, molecules move across the cell membrane...

<p>...from an area of high concentration to an area of low concentration, without requiring ATP (A)</p> Signup and view all the answers

What is the primary difference between simple diffusion and facilitated diffusion?

<p>Simple diffusion does not require a protein carrier or channel, while facilitated diffusion does. (B)</p> Signup and view all the answers

Which type of active transport protein transports a single molecule across the cell membrane?

<p>Uniporters (B)</p> Signup and view all the answers

Which term refers to the liquid portion of the cytoplasm in prokaryotic cells?

<p>Cytosol (D)</p> Signup and view all the answers

In active transport, what role does ATP hydrolysis play?

<p>It provides the energy needed to move molecules against their concentration gradient. (C)</p> Signup and view all the answers

Which of the following is a primary function of the eukaryotic extracellular matrix (ECM)?

<p>Facilitating cell-to-cell communication and adhesion. (D)</p> Signup and view all the answers

If a cell requires a large quantity of glycoproteins to be synthesized and exported, which organelle would likely be most prominent and active?

<p>Golgi Bodies (B)</p> Signup and view all the answers

What is a key difference between eukaryotic and prokaryotic flagella in terms of structure?

<p>Eukaryotic flagella have a '9 + 2' arrangement of microtubules, while prokaryotic flagella are composed of helical chains. (D)</p> Signup and view all the answers

How does the arrangement of microtubules differ between the shaft and basal body of eukaryotic flagella?

<p>The shaft has a '9 + 2' arrangement, while the basal body has a '9 + 0' arrangement. (B)</p> Signup and view all the answers

Which of the following best describes the endomembrane system's role in cellular function?

<p>A transport network between the nucleus and other organelles (A)</p> Signup and view all the answers

What role do cilia play in eukaryotic cells beyond movement of the cell itself?

<p>Moving substances across the cell surface. (A)</p> Signup and view all the answers

How do mitochondria and chloroplasts differ from other organelles within the endomembrane system?

<p>They possess their own DNA and ribosomes (A)</p> Signup and view all the answers

How do prokaryotic cell walls assist some cells in eluding antimicrobial drugs?

<p>By preventing the drugs from reaching their intracellular targets through structural and compositional properties. (C)</p> Signup and view all the answers

A cell is found to be deficient in its ability to break down cellular waste and contaminants. Which organelle is most likely malfunctioning?

<p>Lysosomes (B)</p> Signup and view all the answers

If an antimicrobial drug targets the tetrapeptide cross-bridges in peptidoglycan, which type of bacteria would be MOST affected?

<p>Bacteria with a thick peptidoglycan layer. (A)</p> Signup and view all the answers

Which of the following structures is NOT found in all eukaryotic cells?

<p>Cell Wall (C)</p> Signup and view all the answers

If a plant cell's peroxisomes were not functioning correctly, which process would be most directly affected?

<p>Conversion of storage oils to energy (C)</p> Signup and view all the answers

What distinguishes the ribosomes in eukaryotic cells from those in prokaryotic cells?

<p>Eukaryotic ribosomes are larger (80S) compared to prokaryotic ribosomes (70S). (C)</p> Signup and view all the answers

What is the primary structural difference responsible for Gram-positive bacteria appearing purple after Gram staining, versus Gram-negative bacteria appearing pink?

<p>Gram-positive bacteria cells have a thicker peptidoglycan layer that retains the crystal violet dye. (C)</p> Signup and view all the answers

What would be the immediate consequence if the rough endoplasmic reticulum (RER) in a cell were damaged?

<p>Protein transport to the Golgi bodies would be impaired (D)</p> Signup and view all the answers

How do eukaryotic cells primarily generate ATP?

<p>Within mitochondria through cellular respiration. (A)</p> Signup and view all the answers

Considering the structure of peptidoglycan, what would be the MOST likely effect of an enzyme that specifically breaks the bonds between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG)?

<p>Weakening of the peptidoglycan structure, leading to cell lysis. (D)</p> Signup and view all the answers

Which cellular component is responsible for the synthesis and transport of proteins and lipids in eukaryotic cells?

<p>The endoplasmic reticulum (ER). (D)</p> Signup and view all the answers

A researcher is studying a new eukaryotic cell and observes that it has a double membrane, 70S ribosomes, and is capable of producing ATP. Which organelle is the researcher most likely observing?

<p>Mitochondrion (A)</p> Signup and view all the answers

In bacterial cell walls, what is the function of tetrapeptide cross-bridges that link chains of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)?

<p>To provide structural integrity and rigidity to the peptidoglycan layer. (C)</p> Signup and view all the answers

What is the primary function of the nucleus in a eukaryotic cell?

<p>To house and protect the cell's DNA. (B)</p> Signup and view all the answers

Which organelle is primarily responsible for modifying and packaging proteins into secretory vesicles for export from the cell through exocytosis?

<p>Golgi Body (D)</p> Signup and view all the answers

What is the main component of eukaryotic cell walls?

<p>Polysaccharides (D)</p> Signup and view all the answers

Flashcards

Atom

Smallest unit of matter; composed of protons, neutrons, and electrons.

Proton

Positively charged subatomic particle located in the nucleus.

Neutron

Neutral (no charge) subatomic particle located in the nucleus.

Electron

Negatively charged subatomic particle orbiting the nucleus.

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Chemical Bond

The combining of atoms by sharing or exchanging electrons.

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Ionic Bond

A chemical bond resulting from the attraction between oppositely charged ions.

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Cation

Positively charged ion.

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Anion

Negatively charged ion.

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Covalent Bond

A chemical bond that involves the sharing of electron pairs between atoms.

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Electronegativity

The measure of an atom's attraction for electrons in a chemical bond.

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Nonpolar Covalent Bond

Covalent bond with equal sharing of electrons.

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Polar Covalent Bond

Covalent bond with unequal sharing of electrons, creating partial charges.

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Hydrogen Bond

Weak attraction between partially charged atoms in polar molecules.

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Chemical Reaction

The making or breaking of chemical bonds.

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Synthesis Reaction

Reaction where larger molecules are formed from smaller ones.

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Decomposition Reaction

Reaction where larger molecules are broken down into smaller ones.

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Exchange Reaction

Reaction involving the transfer of atoms or groups between molecules.

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Redox Reactions

Oxidation is loss, reduction is gain of electrons.

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Organic Macromolecules

Large, carbon-containing molecules essential for life.

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Lipids

Class of organic macromolecules including fats, phospholipids, waxes, and sterols.

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Phospholipid

A type of lipid molecule consisting of a glycerol bound to two fatty acids and a phosphate group.

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Carbohydrates

Organic molecules composed of carbon, hydrogen, and oxygen.

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Monosaccharides

Simple sugars; monomers of carbohydrates.

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Disaccharides

Two monosaccharides joined by a glycosidic bond.

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Polysaccharides

Complex carbohydrates composed of many monosaccharide units.

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Proteins

Chains of amino acids joined by peptide bonds.

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Amino Acids

Monomers that make up proteins.

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Peptide Bond

Covalent bond that links amino acids in proteins.

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Nucleic Acids

DNA and RNA; carry genetic information.

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Nucleotides

Monomers of nucleic acids; composed of a sugar, phosphate, and nitrogenous base.

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Microbiology

The study of microorganisms, including bacteria, viruses, fungi, protozoa, and algae.

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Microorganisms

Minute living things, usually too small to be seen with the naked eye; including bacteria, fungi, protozoa, and viruses.

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Virus

A major class of microorganisms; acellular infectious agent that replicates only inside a host cell.

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Ebola

A filovirus that causes severe hemorrhagic fever in humans and primates.

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Contacting the lecturer

To get in touch with the lecturer, face-to-face is most effective.

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Animal Virus

A unique type of microorganism that infects animal cells.

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Plant Virus

A unique type of microorganism that infects plant cells.

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Bacterial Virus

A unique type of microorganism that infects bacterial cells

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Influenza Virus

A virus known to cause the flu.

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Canine Parvovirus

A virus known to cause a highly contagious disease in dogs.

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Coronavirus

A virus with a crown-like appearance that infects mammals and birds.

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T4 Phage

A virus that infects bacteria (E. coli) .

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Algae

Microorganisms that are unicellular or multicellular and photosynthetic.

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Archaea

Microorganisms that often thrive in extreme environments like high heat, pressure, or salt.

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Spirillum

Spiral-shaped bacteria.

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Theory of Spontaneous Generation

The idea that living organisms can arise from non-living matter.

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Plant Domestication

Using plants for food production.

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Plagues

Infectious diseases that affected people in ancient history.

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Syphilis

Caused by Treponema pallidum.

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Metabolism

Ability to derive energy from molecules.

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Era of Speculation

The period in history when people began interacting with microorganisms without understanding them.

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Koch's 3rd Postulate

Agent must be isolated and grown outside the host as a step to identify disease using Koch's postulates.

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Koch's 4th Postulate

Introducing the isolated agent into a healthy host will reproduce the original disease.

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Koch's Postulates: Reisolation

The agent must be re-isolated from the inoculated, diseased host.

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Koch's Postulates Limitation

Not all microorganisms can be grown outside a host

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Ignaz Semmelweis

Observed the mortality rate of childbirth and implemented hand washing.

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Spontaneous Generation

The idea that life arises from non-living matter; popularized by Greek philosophers.

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Environment & Disease Link

Early thinkers connected environment to disease, but lacked tools to identify microbes.

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Era of Observation

A period marked by the use of early microscopes to visualize microorganisms.

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Antoni van Leeuwenhoek

Dutch scientist who made microscopes and visualized bacteria and protozoa, calling them 'animalcules'.

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Early Taxonomy

An outdated approach to classifying organisms using lengthy, variable descriptions.

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Leeuwenhoek's Titles

The scientist who is credited as the 'father of protozoology' and 'father of bacteriology'.

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Communicable Diseases (Ancient)

In ancient times, recognizing that some diseases could spread from person to person.

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Wine Preservation

Practices used to prevent the spoilage of the grape juice to make wine.

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Scientific Method

A systematic approach to understanding the natural world through observation, questioning, hypothesis formation, experimentation, and analysis.

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Pasteurization

A process of heating food or beverages to kill harmful microorganisms and prevent spoilage.

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Louis Pasteur

Father of Microbiology, disproved spontaneous generation, and developed pasteurization.

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Fermentation

A biological process where microorganisms cause chemical changes in a substance (e.g., converting sugar to alcohol).

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Germ Theory of Disease

The principle that microorganisms can invade the body and cause disease.

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Koch’s Postulates

A set of criteria to establish that an agent causes a particular disease.

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Koch's Postulate #1

A criterion where the suspected agent must be found in every case of the disease and absent in healthy hosts.

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Light Microscopes

Microscopes that use visible or UV light to visualize specimens.

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Electron Microscopes

Microscopes that employ a beam of electrons to create an image.

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Scanning Probe Microscopes

Microscopes that use a physical probe to scan the surface of a sample.

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Resolution

The ability of an optical instrument to separate or distinguish between small objects that are close together.

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Wavelength and Resolution

Shorter wavelengths provide greater resolving power in microscopy.

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Refraction

Bending of light or radiation as it passes through a medium.

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Magnification

The perceived enlargement of an image.

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Contrast

The difference in intensity between two objects or an object and its background.

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Staining

Coloring specimens with dyes to improve contrast and resolution.

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Basic Dyes

Dyes with a positive charge (+ charge) that stain acidic structures.

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Bright-Field Microscope

A microscope with one or two ocular lenses and multiple objective lenses.

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Oil Immersion Lens

Increase resolution by reducing light refraction.

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Variable Spirilli

Rod-shaped bacteria that can vary in shape (pleomorphic).

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Bacterial Arrangement Factors

Plane of cell division and separation of daughter cells.

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Binary Fission

Asexual reproduction where a cell replicates its DNA and divides into two identical daughter cells.

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Snapping Division

A variation of binary fission in Gram-positive bacteria where the outer cell wall snaps due to tension, leaving daughter cells attached.

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Cocci Arrangement

Cell division plane and daughter cell separation.

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Dark-Field Microscopy

Specimen appears light against a dark background, increasing contrast for detailed observation.

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Fluorescence Microscopy

Uses high-intensity light (UV) to illuminate fluorescent molecules in the specimen. The specimen appears colored against a black background.

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Confocal Microscopy

Creates high-resolution images by scanning a specimen with a focused laser beam; can create 3D reconstructions.

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Transmission Electron Microscope (TEM)

Type of electron microscope where electrons pass through a thin specimen to create a detailed image of internal structures.

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Scanning Electron Microscope (SEM)

Type of electron microscope that scans the surface of a specimen with electrons to create a 3D image of the surface.

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Electron Microscope uses

Provides detailed views of bacteria, viruses, internal cellular structures, molecules, and large atoms

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Electron Microscope Advantage

Uses electrons with short-wavelength providing HUGE boosts in both resolving power and magnification

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Staphylococci

Spherical-shaped bacteria arranged in irregular clusters.

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Diplococci

Spherical-shaped bacteria arranged in pairs.

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Sarcina

Spherical-shaped bacteria arranged in cubical packets of eight.

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Diplobacilli

Rod-shaped bacteria arranged in pairs.

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Endospores

Specialized dormant structures produced by Gram-positive Bacillus and Clostridium species

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Scanning Probe/AFM Microscopes

Microscopes using a pointed probe to scan surfaces at the atomic level.

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Simple Stains

Use of one dye type to add color to cells.

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Differential Stains

Stains that differentiate bacteria based on cell wall composition.

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Gram Stain

A differential stain that distinguishes bacteria based on cell wall structure (Gram-positive or Gram-negative).

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Gram Stain Steps

Step 1: Crystal violet (purple) Step 2: Iodine (mordant) Step 3: Alcohol (decolorize) Step 4: Safranin (red dye)

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Prokaryotic Cell Shape

Spherical (coccus), Rodlike (bacillus), Bent Rods (vibrio), Corkscrew (spirillum)

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Outer Membrane (Bacteria)

A membrane found in Gram-negative bacteria, containing phospholipids, porins, and lipopolysaccharide (LPS).

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Gram-Negative Stain Color

The staining result of Gram-negative cells after Gram staining, due to safranin dye.

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Cell Membrane Function

The cell membrane is composed of lipids and proteins that controls the movement of substances into and out of the cell and facilitates the transport of molecules.

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Selectively Permeable Membrane

A selective barrier that controls the passage of substances into and out of the cell.

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Passive Transport

Movement across a membrane without energy input, from high to low concentration.

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Simple Diffusion

Movement of molecules, requires no energy (ATP), from an area of high concentration to an area of low concentration.

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Osmosis

The simple diffusion of water molecules from high concentration to low concentration.

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Isotonic Solution

Solution with equal solute concentration inside and outside the cell.

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Hypertonic Solution

Solution with higher solute concentration outside the cell, causing water to leave the cell.

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Hypotonic Solution

Solution with lower solute concentration outside the cell, causing water to enter the cell.

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Cytoskeleton

Network of protein fibers providing cell shape, organelle anchoring, movement, and membrane dynamics.

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Microtubules

Composed of tubulin protein, these are one of the three structural elements of the cytoskeleton.

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Microfilaments

Composed of actin protein, these are one of the three structural elements of the cytoskeleton.

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Ribosomes

Contain RNA and protein, these are essential for protein synthesis (translation).

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Nucleus

Double-membrane organelle containing most of the cell's DNA, surrounded by the nuclear envelope.

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Enzymes (Eukaryotic)

Catalyze chemical reactions within cells.

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DNA/RNA (Eukaryotic)

Genetic information storage and transfer.

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Metabolic Cycles (Eukaryotic)

Series of chemical reactions that extract energy from molecules.

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Extracellular Matrices (Eukaryotic)

Anchor to surface/strengthens cell surface.

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Flagella/Cilia (Eukaryotic)

Movement of cell or substances.

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Nucleus (Eukaryotic)

Houses DNA of the cell.

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Ribosomes (Eukaryotic)

Sites for protein synthesis (80S).

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Endoplasmic Reticulum (Eukaryotic)

Synthesis, processing, and transport of proteins and lipids.

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Mitochondria (Eukaryotic)

Produce most of the cell’s ATP (energy).

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Chloroplasts (Eukaryotic)

Light-harvesting structures for making ATP.

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MIC 205

Microbiology course at ASU

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Patrick Daydif

Name of the instructor

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Office Location

UCENT 356

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Phone Number

(602) 496-0599

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Email Address

[email protected]

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Smooth Endoplasmic Reticulum (SER)

Involved in lipid synthesis. Lacks ribosomes.

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Rough Endoplasmic Reticulum (RER)

Has ribosomes attached; transports proteins to Golgi bodies.

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Golgi Bodies

Receives, processes, and packages proteins into vesicles for export.

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Endomembrane System

A network of membranes including ER, Golgi, and lysosomes.

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Mitochondria

Produces most of the cell's ATP; contains its own DNA and ribosomes.

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Chloroplasts

Harvests light energy for ATP synthesis in photosynthetic eukaryotes.

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Lysosomes

Contain enzymes for breaking down cellular waste.

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Vacuoles and Vesicles

Store and transfer chemicals/nutrients within cells.

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Flagella Function

Filamentous structures used for motility.

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Pili and Fimbriae

Attachment structures on the surface of some prokaryotes.

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Prokaryotic Cell Wall Function

Provides structure, shape, and protection from osmotic forces; assists in attachment.

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Peptidoglycan Composition

Peptide-linked chains of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM).

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Gram-Positive Cell Walls

Thick peptidoglycan layer that retains crystal violet dye, staining cells purple.

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Study Notes

  • Electrons move in shells around the nucleus
  • Atoms will share or exchange electrons when close to each other
  • Outer energy shells stabilizing is determined by the stability of the atom

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