Atomic Structure and Quantum Mechanics
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Questions and Answers

What is the main function of a ddNTP in DNA sequencing?

  • To increase the GC content of the target DNA strand
  • To terminate the DNA chain and mark its end (correct)
  • To facilitate the binding of primers to the template DNA
  • To provide a hydroxyl group for nucleotide addition

    • What is the main characteristic of the stationary phase in liquid chromatography?

  • It is used to separate molecules based on their size
  • It is heat-tolerant and most active around 70°C
  • It is polar and elutes slower (correct)
  • It is nonpolar and elutes faster

    • What is the primary purpose of the primer in PCR?

  • To prevent the formation of secondary structures in the template DNA
  • To facilitate the annealing of the primer to the target DNA region (correct)
  • To provide a binding site for the Taq polymerase
  • To increase the melting temperature of the template DNA

    • What is the purpose of the heating and cooling cycles in PCR?

    <p>To denature the template DNA and anneal the primers</p> Signup and view all the answers

    What is the optimal temperature for the activity of Taq polymerase?

    <p>70°C</p> Signup and view all the answers

    What is the primary function of gel electrophoresis in DNA sequencing?

    <p>To separate DNA strands based on their size</p> Signup and view all the answers

    What is the main characteristic of the mobile phase in liquid chromatography?

    <p>It is nonpolar and elutes faster</p> Signup and view all the answers

    What is the key to designing a primer for PCR?

    <p>High GC content and a G or C at each end</p> Signup and view all the answers

    What is the name of the bacterium from which Taq polymerase is isolated?

    <p>Thermos aquaticus</p> Signup and view all the answers

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