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Questions and Answers
What is the main function of a ddNTP in DNA sequencing?
What is the main function of a ddNTP in DNA sequencing?
- To increase the GC content of the target DNA strand
- To terminate the DNA chain and mark its end (correct)
- To facilitate the binding of primers to the template DNA
- To provide a hydroxyl group for nucleotide addition
What is the main characteristic of the stationary phase in liquid chromatography?
What is the main characteristic of the stationary phase in liquid chromatography?
- It is used to separate molecules based on their size
- It is heat-tolerant and most active around 70°C
- It is polar and elutes slower (correct)
- It is nonpolar and elutes faster
What is the primary purpose of the primer in PCR?
What is the primary purpose of the primer in PCR?
- To prevent the formation of secondary structures in the template DNA
- To facilitate the annealing of the primer to the target DNA region (correct)
- To provide a binding site for the Taq polymerase
- To increase the melting temperature of the template DNA
What is the purpose of the heating and cooling cycles in PCR?
What is the purpose of the heating and cooling cycles in PCR?
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What is the optimal temperature for the activity of Taq polymerase?
What is the optimal temperature for the activity of Taq polymerase?
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What is the primary function of gel electrophoresis in DNA sequencing?
What is the primary function of gel electrophoresis in DNA sequencing?
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What is the main characteristic of the mobile phase in liquid chromatography?
What is the main characteristic of the mobile phase in liquid chromatography?
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What is the key to designing a primer for PCR?
What is the key to designing a primer for PCR?
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What is the name of the bacterium from which Taq polymerase is isolated?
What is the name of the bacterium from which Taq polymerase is isolated?
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