Aseptic Techniques and Inoculation Method Quiz

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10 Questions

What is the purpose of aseptic techniques in microbiology?

To prevent contamination of specimens

Why is flaming advised before closing a vessel in microbiological work?

To sterilize the opening of the vessel

Which type of cabinet is commonly used in college laboratories for certain microbiological work?

Class II laminar flow BSC

What action should be taken if the contents of a vessel in microbiological work are likely to catch fire?

Ignore the risk and close it quickly

How can the risk of contamination in a laboratory be further reduced?

Treating the working table with hypochlorite

What is the primary purpose of a Class III biosafety cabinet?

To work with highly pathogenic microorganisms

What is the recommended method for sterilizing an inoculating loop or needle?

Flaming the entire wire portion to red hot

What is the purpose of not shaking the inoculating loop or needle while cooling after flaming?

To avoid aerosol formation

What factor determines the size of the inoculum obtained from a liquid microbial suspension using an inoculating loop?

Both the concentration of cells and the size of the loop

How can aerosol formation during flaming be avoided?

By flaming the wire portion in the inner part of the flame

This quiz covers aseptic techniques used to prevent contamination of specimens and microorganisms. Learn about handling sterile materials, keeping vessels closed, and containing microorganisms in their appropriate surroundings.

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