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Aseptic Techniques and Inoculation Method Quiz

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Questions and Answers

What is the purpose of aseptic techniques in microbiology?

To prevent contamination of specimens (correct)
To increase the growth of bacteria
To mix different specimens together
To create a pleasant working environment

Why is flaming advised before closing a vessel in microbiological work?

To ensure the vessel catches fire easily
To increase the risk of contamination
To sterilize the opening of the vessel (correct)
To prevent the entry of filtered air

Which type of cabinet is commonly used in college laboratories for certain microbiological work?

Class II laminar flow BSC (correct)
Class II biosafety cabinet
Class I laminar flow BSC
Class III biosafety cabinet

What action should be taken if the contents of a vessel in microbiological work are likely to catch fire?

Ignore the risk and close it quickly (C) Signup and view all the answers

How can the risk of contamination in a laboratory be further reduced?

Treating the working table with hypochlorite (C) Signup and view all the answers

What is the primary purpose of a Class III biosafety cabinet?

To work with highly pathogenic microorganisms (D) Signup and view all the answers

What is the recommended method for sterilizing an inoculating loop or needle?

Flaming the entire wire portion to red hot (C) Signup and view all the answers

What is the purpose of not shaking the inoculating loop or needle while cooling after flaming?

To avoid aerosol formation (D) Signup and view all the answers

What factor determines the size of the inoculum obtained from a liquid microbial suspension using an inoculating loop?

Both the concentration of cells and the size of the loop (B) Signup and view all the answers

How can aerosol formation during flaming be avoided?

By flaming the wire portion in the inner part of the flame (D) Signup and view all the answers

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