5 Questions
When cleansing the skin for blood culture collection, what is the correct order of solution application?
70% alcohol and then 2% iodine or an iodophor
How long should the iodine (or iodophor) remain intact on the skin during blood culture collection?
30 sec
What is the purpose of adding 0.025%-0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for blood culture collection?
It inhibits phagocytosis and complement.
Which organism is best recovered from the nasopharynx using a flexible calcium alginate nasopharyngeal swab?
Streptococcus pneumoniae
What is the purpose of semisolid transport media such as Amies, Stuart, or Cary-Blair in blood culture collection?
It functions as a preservative.
Study Notes
Blood Culture Collection
- The correct order of solution application for skin cleansing is: scrub with antimicrobial soap and water, then apply an iodine or iodophor solution, and finally apply 70% isopropyl alcohol.
Iodine Application
- Iodine or iodophor solution should remain intact on the skin for 30 seconds to 1 minute during blood culture collection.
Nutrient Broth Media
- Adding 0.025%-0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for blood culture collection allows for the growth of certain fastidious bacteria.
Nasopharyngeal Swab
- Flexible calcium alginate nasopharyngeal swabs are used to recover Bordetella pertussis from the nasopharynx.
Transport Media
- Semisolid transport media such as Amies, Stuart, or Cary-Blair are used to maintain the viability of microorganisms during transportation, particularly for blood culture collection.
Test your knowledge of aseptic blood culture collection with this quiz. From skin cleansing to the duration of iodine application, challenge yourself with questions on proper procedures for aseptic blood culture collection.
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