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Questions and Answers
When cleansing the skin for blood culture collection, what is the correct order of solution application?
When cleansing the skin for blood culture collection, what is the correct order of solution application?
- 2% iodine and then 70% alcohol solution
- 95% alcohol only
- 70% alcohol and then 2% iodine or an iodophor (correct)
- 70% alcohol and then 95% alcohol
How long should the iodine (or iodophor) remain intact on the skin during blood culture collection?
How long should the iodine (or iodophor) remain intact on the skin during blood culture collection?
- 10 sec
- 60 sec
- 5 min
- 30 sec (correct)
What is the purpose of adding 0.025%-0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for blood culture collection?
What is the purpose of adding 0.025%-0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for blood culture collection?
- It inhibits phagocytosis and complement. (correct)
- It promotes formation of a blood clot.
- It enhances growth of anaerobes.
- It functions as a preservative.
Which organism is best recovered from the nasopharynx using a flexible calcium alginate nasopharyngeal swab?
Which organism is best recovered from the nasopharynx using a flexible calcium alginate nasopharyngeal swab?
What is the purpose of semisolid transport media such as Amies, Stuart, or Cary-Blair in blood culture collection?
What is the purpose of semisolid transport media such as Amies, Stuart, or Cary-Blair in blood culture collection?
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Study Notes
Blood Culture Collection
- The correct order of solution application for skin cleansing is: scrub with antimicrobial soap and water, then apply an iodine or iodophor solution, and finally apply 70% isopropyl alcohol.
Iodine Application
- Iodine or iodophor solution should remain intact on the skin for 30 seconds to 1 minute during blood culture collection.
Nutrient Broth Media
- Adding 0.025%-0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for blood culture collection allows for the growth of certain fastidious bacteria.
Nasopharyngeal Swab
- Flexible calcium alginate nasopharyngeal swabs are used to recover Bordetella pertussis from the nasopharynx.
Transport Media
- Semisolid transport media such as Amies, Stuart, or Cary-Blair are used to maintain the viability of microorganisms during transportation, particularly for blood culture collection.
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