Are you a tRNA charging expert?
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Questions and Answers

What is the definition of charging of tRNA?

  • Binding of an amino acid with a cognate tRNA (correct)
  • Binding of a lipid with a cognate tRNA
  • Binding of a protein with a cognate tRNA
  • Binding of a nucleotide with a cognate tRNA

What is the role of aa-tRNA synthetases in charging of tRNA?

  • They catalyze the binding of a nucleotide with a cognate tRNA
  • They catalyze the binding of an amino acid with a cognate tRNA (correct)
  • They catalyze the binding of a protein with a cognate tRNA
  • They catalyze the binding of a lipid with a cognate tRNA

How many aa-tRNA synthetases or charging enzymes are there?

  • Exactly 100
  • Less than 10
  • More than 50
  • Approximately 22 (correct)

What do aa-tRNA produce?

<p>Amino acyl tRNA (B)</p> Signup and view all the answers

What is the reactivity of aa-tRNAs?

<p>Highly reactive &amp; unstable (B)</p> Signup and view all the answers

What is the energy requirement for charging of tRNA?

<p>All of the above (D)</p> Signup and view all the answers

What is the maximum energy requirement for the proof-reading activity in translation?

<p>ATP (C)</p> Signup and view all the answers

What is the enzyme responsible for charging of tRNA called?

<p>aa-tRNA synthetase (C)</p> Signup and view all the answers

What do aa-tRNAs serve as?

<p>Active precursors for translation (D)</p> Signup and view all the answers

What is the definition of charging of tRNA?

<p>Binding of an amino acid with a cognate tRNA (C)</p> Signup and view all the answers

What is the role of aa-tRNA synthetases in charging of tRNA?

<p>They catalyze the binding of an amino acid with a cognate tRNA (C)</p> Signup and view all the answers

How many aa-tRNA synthetases or charging enzymes are there?

<p>Approximately 22 (C)</p> Signup and view all the answers

What do aa-tRNA produce?

<p>Amino acyl tRNA (B)</p> Signup and view all the answers

What is the reactivity of aa-tRNAs?

<p>Highly reactive &amp; unstable (C)</p> Signup and view all the answers

What is the energy requirement for charging of tRNA?

<p>Amino acid + ATP + tRNA ⤑ E⤑ aa-tRNA + AMP + 2Pi (D)</p> Signup and view all the answers

What is the proof-reading activity of the charging enzyme?

<p>Binding of an amino acid with a non-cognate tRNA (B)</p> Signup and view all the answers

What is the maximum energy requirement for the proof-reading activity in translation?

<p>ATP (C)</p> Signup and view all the answers

What is the enzyme responsible for charging of tRNA called?

<p>aa-tRNA synthetase (B)</p> Signup and view all the answers

What do aa-tRNAs serve as?

<p>Active precursors for translation (D)</p> Signup and view all the answers

What is charging of tRNA?

<p>Binding of an amino acid with a cognate tRNA (C)</p> Signup and view all the answers

What is the role of aa-tRNA synthetases in the charging of tRNA?

<p>Catalyze the binding of an amino acid with a cognate tRNA (C)</p> Signup and view all the answers

How many aa-tRNA synthetases or charging enzymes are there?

<p>More than 22 (D)</p> Signup and view all the answers

What is produced by the aa-tRNA synthetases or charging enzymes?

<p>Amino acyl tRNA (aa-tRNA) (D)</p> Signup and view all the answers

Why are aa-tRNAs highly reactive and unstable?

<p>Because they serve as active precursors for translation (C)</p> Signup and view all the answers

What is the energy requirement for charging of tRNA?

<p>Amino acid + ATP (D)</p> Signup and view all the answers

What is the proof-reading activity of the charging enzyme?

<p>Ensures proper attachment of amino acid to tRNA (C)</p> Signup and view all the answers

What is the maximum energy requirement for the proof-reading activity of the charging enzyme?

<p>ATP (D)</p> Signup and view all the answers

What is the role of aa-tRNAs in translation?

<p>Serve as active precursors for translation (B)</p> Signup and view all the answers

What is E/Enzyme in the context of the charging enzyme?

<p>aa tRNA synthetase or charging enzyme (A)</p> Signup and view all the answers

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