29 Questions
What is the definition of charging of tRNA?
Binding of an amino acid with a cognate tRNA
What is the role of aa-tRNA synthetases in charging of tRNA?
They catalyze the binding of an amino acid with a cognate tRNA
How many aa-tRNA synthetases or charging enzymes are there?
Approximately 22
What do aa-tRNA produce?
Amino acyl tRNA
What is the reactivity of aa-tRNAs?
Highly reactive & unstable
What is the energy requirement for charging of tRNA?
All of the above
What is the maximum energy requirement for the proof-reading activity in translation?
ATP
What is the enzyme responsible for charging of tRNA called?
aa-tRNA synthetase
What do aa-tRNAs serve as?
Active precursors for translation
What is the definition of charging of tRNA?
Binding of an amino acid with a cognate tRNA
What is the role of aa-tRNA synthetases in charging of tRNA?
They catalyze the binding of an amino acid with a cognate tRNA
How many aa-tRNA synthetases or charging enzymes are there?
Approximately 22
What do aa-tRNA produce?
Amino acyl tRNA
What is the reactivity of aa-tRNAs?
Highly reactive & unstable
What is the energy requirement for charging of tRNA?
Amino acid + ATP + tRNA ⤑ E⤑ aa-tRNA + AMP + 2Pi
What is the proof-reading activity of the charging enzyme?
Binding of an amino acid with a non-cognate tRNA
What is the maximum energy requirement for the proof-reading activity in translation?
ATP
What is the enzyme responsible for charging of tRNA called?
aa-tRNA synthetase
What do aa-tRNAs serve as?
Active precursors for translation
What is charging of tRNA?
Binding of an amino acid with a cognate tRNA
What is the role of aa-tRNA synthetases in the charging of tRNA?
Catalyze the binding of an amino acid with a cognate tRNA
How many aa-tRNA synthetases or charging enzymes are there?
More than 22
What is produced by the aa-tRNA synthetases or charging enzymes?
Amino acyl tRNA (aa-tRNA)
Why are aa-tRNAs highly reactive and unstable?
Because they serve as active precursors for translation
What is the energy requirement for charging of tRNA?
Amino acid + ATP
What is the proof-reading activity of the charging enzyme?
Ensures proper attachment of amino acid to tRNA
What is the maximum energy requirement for the proof-reading activity of the charging enzyme?
ATP
What is the role of aa-tRNAs in translation?
Serve as active precursors for translation
What is E/Enzyme in the context of the charging enzyme?
aa tRNA synthetase or charging enzyme
Test your knowledge on the process of charging tRNA with this quiz! Explore the mechanisms and enzymes involved in binding amino acids to their cognate tRNA molecules. Learn about the importance of amino acyl tRNAs as active precursors for translation and the energy requirements for tRNA charging. Don't miss this opportunity to deepen your understanding of this fundamental step in protein synthesis!
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