Are you a tRNA charging expert?
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Questions and Answers

What is the definition of charging of tRNA?

  • Binding of an amino acid with a cognate tRNA (correct)
  • Binding of a lipid with a cognate tRNA
  • Binding of a protein with a cognate tRNA
  • Binding of a nucleotide with a cognate tRNA

    • What is the role of aa-tRNA synthetases in charging of tRNA?

  • They catalyze the binding of a nucleotide with a cognate tRNA
  • They catalyze the binding of an amino acid with a cognate tRNA (correct)
  • They catalyze the binding of a protein with a cognate tRNA
  • They catalyze the binding of a lipid with a cognate tRNA

    • How many aa-tRNA synthetases or charging enzymes are there?

  • Exactly 100
  • Less than 10
  • More than 50
  • Approximately 22 (correct)

    • What do aa-tRNA produce?

    <p>Amino acyl tRNA</p> Signup and view all the answers

    What is the reactivity of aa-tRNAs?

    <p>Highly reactive &amp; unstable</p> Signup and view all the answers

    What is the energy requirement for charging of tRNA?

    <p>All of the above</p> Signup and view all the answers

    What is the maximum energy requirement for the proof-reading activity in translation?

    <p>ATP</p> Signup and view all the answers

    What is the enzyme responsible for charging of tRNA called?

    <p>aa-tRNA synthetase</p> Signup and view all the answers

    What do aa-tRNAs serve as?

    <p>Active precursors for translation</p> Signup and view all the answers

    What is the definition of charging of tRNA?

    <p>Binding of an amino acid with a cognate tRNA</p> Signup and view all the answers

    What is the role of aa-tRNA synthetases in charging of tRNA?

    <p>They catalyze the binding of an amino acid with a cognate tRNA</p> Signup and view all the answers

    How many aa-tRNA synthetases or charging enzymes are there?

    <p>Approximately 22</p> Signup and view all the answers

    What do aa-tRNA produce?

    <p>Amino acyl tRNA</p> Signup and view all the answers

    What is the reactivity of aa-tRNAs?

    <p>Highly reactive &amp; unstable</p> Signup and view all the answers

    What is the energy requirement for charging of tRNA?

    <p>Amino acid + ATP + tRNA ⤑ E⤑ aa-tRNA + AMP + 2Pi</p> Signup and view all the answers

    What is the proof-reading activity of the charging enzyme?

    <p>Binding of an amino acid with a non-cognate tRNA</p> Signup and view all the answers

    What is the maximum energy requirement for the proof-reading activity in translation?

    <p>ATP</p> Signup and view all the answers

    What is the enzyme responsible for charging of tRNA called?

    <p>aa-tRNA synthetase</p> Signup and view all the answers

    What do aa-tRNAs serve as?

    <p>Active precursors for translation</p> Signup and view all the answers

    What is charging of tRNA?

    <p>Binding of an amino acid with a cognate tRNA</p> Signup and view all the answers

    What is the role of aa-tRNA synthetases in the charging of tRNA?

    <p>Catalyze the binding of an amino acid with a cognate tRNA</p> Signup and view all the answers

    How many aa-tRNA synthetases or charging enzymes are there?

    <p>More than 22</p> Signup and view all the answers

    What is produced by the aa-tRNA synthetases or charging enzymes?

    <p>Amino acyl tRNA (aa-tRNA)</p> Signup and view all the answers

    Why are aa-tRNAs highly reactive and unstable?

    <p>Because they serve as active precursors for translation</p> Signup and view all the answers

    What is the energy requirement for charging of tRNA?

    <p>Amino acid + ATP</p> Signup and view all the answers

    What is the proof-reading activity of the charging enzyme?

    <p>Ensures proper attachment of amino acid to tRNA</p> Signup and view all the answers

    What is the maximum energy requirement for the proof-reading activity of the charging enzyme?

    <p>ATP</p> Signup and view all the answers

    What is the role of aa-tRNAs in translation?

    <p>Serve as active precursors for translation</p> Signup and view all the answers

    What is E/Enzyme in the context of the charging enzyme?

    <p>aa tRNA synthetase or charging enzyme</p> Signup and view all the answers

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